LOCUS       MTK3_027                3924 bp ds-DNA     circular     21-FEB-2019
DEFINITION  .
FEATURES             Location/Qualifiers
     feature         3071..3089
                     /label="FLAG"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    complement(3105..3213)
                     /label="CamR Terminator"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     feature         1747..2162
                     /label="Gal4_DBD"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     feature         3066..3070
                     /label="FLAG"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     feature         856..857
                     /label="NLS"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    834..1658
                     /label="ABA1"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    2163..3065
                     /label="ABIcs"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     rep_origin      complement(55..818)
                     /label="ColE1"
                     /ApEinfo_revcolor=#7f7f7f
                     /ApEinfo_fwdcolor=#7f7f7f
     feature         837..855
                     /label="SV40 NLS"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_signal     1662..1724
                     /label="T2A"
                     /ApEinfo_revcolor=#0080ff
                     /ApEinfo_fwdcolor=#0080ff
     feature         856..857
                     /label="SV40 NLS"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     primer          2126..2144
                     /label="Gal4_N_term_primer"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     feature         1725..1746
                     /label="Gal4_DBD"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     feature         1632..1635
                     /label="HA"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     CDS             complement(3214..3873)
                     /label="CamR"
                     /ApEinfo_revcolor=#0000ff
                     /ApEinfo_fwdcolor=#0000ff
     promoter        complement(join(3874..3924,1..54))
                     /label="CamR Promoter"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     feature         843..855
                     /label="NLS"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
ORIGIN
        1 tagtgatctt atttcattat ggtgaaagtt ggaacctctt acgtgcccga tcaatcatga
       61 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
      121 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
      181 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
      241 taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag
      301 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
      361 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
      421 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
      481 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
      541 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
      601 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
      661 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
      721 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
      781 tctttcctgc gttatcccct gattctgtgg ataaccgtag tcggTCTCAt atgGGCCCTA
      841 AAAAGAAGCG TAAAGtcgcc cccccgaccg atgtcagcct gggggacgag ctccacttag
      901 acggcgagga cgtggcgatg gcgcatgccg acgcgctaga cgatttcgat ctggacatgt
      961 tgggggacgg ggattccccg ggtccgggat ttacccccca cgactccgcc ccctacggcg
     1021 ctctggatat ggccgacttc gagtttgagc agatgtttac cgatgccctt ggaattgacg
     1081 agtacggtgg gggcgcgcca actcaagacg aattcaccca actctcccaa tcaatcgccg
     1141 agttccacac gtaccaactc ggtaacggcc gttgctcatc tctcctagct cagcgaatcc
     1201 acgcgccgcc ggaaacagta tggtccgtgg tcagacgttt cgataggcca cagatttaca
     1261 aacacttcat caaaagctgt aacgtgagtg aagatttcga gatgcgagtg ggatgcacgc
     1321 gcgacgtgaa cgtgataagt ggattaccgg cgaatacgtc Acgagagaga ttagatctgt
     1381 tggacgatga tcggagagtg actgggttta gtataaccgg tggtgaacat aggctgagga
     1441 attataaatc ggttacgacg gttcatagat ttgagaaaga agaagaagaa gaaaggatct
     1501 ggaccgttgt tttggaatct tatgttgttg atgtaccgga aggtaattcg gaggaagata
     1561 cgagattgtt tgctgatacg gttattagat tgaatcttca gaaacttgct tcgatcactg
     1621 aagctatgaa ctacccatac gatgttccag attacgcTAG CGGAAGCGGA GAGGGCAGAG
     1681 GAAGTCTGCT AACATGCGGT GACGTCGAGG AGAATCCTGG ACCTaagcta ctgtcttcta
     1741 tcgaacaagc atgcgatatt tgccgactta aaaagctcaa gtgctccaaa gaaaaaccga
     1801 agtgcgccaa gtgtctgaag aacaactggg agtgtcgcta ctctcccaaa accaaaaggt
     1861 caccgctgac tagggcacat ctgacagaag tggaatcaag gctagaaaga ctggaacagc
     1921 tatttctact gatttttcct cgagaagacc ttgacatgat tttgaaaatg gattctttac
     1981 aggatataaa agcattgtta acaggattat ttgtacaaga taatgtgaat aaagatgccg
     2041 tcacagatag attggcttca gtggagactg atatgcctct aacattgaga cagcatagaa
     2101 taagtgcgac atcatcatcg gaagagagta gtaacaaagg tcaaagacag ttgactgtat
     2161 cgACGCGTat gGTGCCTTTG TATGGTTTTA CTTCGATTTG TGGAAGAAGA CCTGAGATGG
     2221 AAGcTGCTGT TTCGACTATA CCAAGATTCC TTCAATCTTC CTCTGGTTCG ATGTTAGATG
     2281 GTCGGTTTGA TCCTCAATCC GCCGCTCATT TCTTCGGTGT TTACGACGGC CATGGCGGTT
     2341 CTCAGGTAGC GAACTATTGT AGAGAGAGGA TGCATTTGGC TTTGGCGGAG GAGATAGCTA
     2401 AGGAGAAACC GATGCTCTGC GATGGTGATA CGTGGCTGGA GAAGTGGAAG AAAGCTCTTT
     2461 TCAACTCGTT CCTGAGAGTT GACTCGGAGA TTGAGTCAGT TGCGCCGGAG ACaGTTGGGT
     2521 CAACGTCGGT GGTTGCCGTT GTTTTCCCGT CACACATCTT CGTCGCTAAC TGCGGTGACT
     2581 CTAGAGCCGT TCTTTGCCGC GGCAAAACTG CACTTCCATT ATCCGTTGAC CATAAACCGG
     2641 ATAGAGAAGA TGAAGCTGCG AGGATTGAAG CCGCAGGAGG GAAAGTGATT CAGTGGAATG
     2701 GAGCTCGTGT TTTCGGTGTT CTCGCCATGT CGAGATCCAT TGGCGATAGA TACTTGAAAC
     2761 CATCCATCAT TCCTGATCCG GAAGTGACGG CTGTGAAGAG AGTAAAAGAA GATGATTGTC
     2821 TGATTTTGGC GAGTGACGGG GTTTGGGATG TAATGACGGA TGAAGAAGCG TGTGAGATGG
     2881 CAAGGAAGCG GATTCTCTTG TGGCACAAGA AAAACGCGGT GGCTGGGGAT GCATCGTTGC
     2941 TCGCGGATGA GCGGAGAAAG GAAGGGAAAG ATCCTGCGGC GATGTCCGCG GCTGAGTATT
     3001 TGTCAAAGCT GGCGATACAG AGAGGAAGCA AAGACAACAT AAGTGTGGTG GTGGTTGATT
     3061 TGAAGGATTA CAAGGACGAT GACGATAAGg gatcctgaga ccagaccaat aaaaaacgcc
     3121 cggcggcaac cgagcgttct gaacaaatcc agatggagtt ctgaggtcat tactggatct
     3181 atcaacagga gtccaagcga gctcgatatc aaattacgcc ccgccctgcc actcatcgca
     3241 gtactgttgt aattcattaa gcattctgcc gacatggaag ccatcacaaa cggcatgatg
     3301 aacctgaatc gccagcggca tcagcacctt gtcgccttgc gtataatatt tgcccatggt
     3361 gaaaacgggg gcgaagaagt tgtccatatt ggccacgttt aaatcaaaac tggtgaaact
     3421 cacccaggga ttggctgaaa cgaaaaacat attctcaata aaccctttag ggaaataggc
     3481 caggttttca ccgtaacacg ccacatcttg cgaatatatg tgtagaaact gccggaaatc
     3541 gtcgtggtat tcactccaga gcgatgaaaa cgtttcagtt tgctcatgga aaacggtgta
     3601 acaagggtga acactatccc atatcaccag ctcaccgtct ttcattgcca tacgaaattc
     3661 cggatgagca ttcatcaggc gggcaagaat gtgaataaag gccggataaa acttgtgctt
     3721 atttttcttt acggtcttta aaaaggccgt aatatccagc tgaacggtct ggttataggt
     3781 acattgagca actgactgaa atgcctcaaa atgttcttta cgatgccatt gggatatatc
     3841 aacggtggta tatccagtga tttttttctc cattttagct tccttagctc ctgaaaatct
     3901 cgataactca aaaaatacgc ccgg
//