LOCUS       MTK3_029                3360 bp ds-DNA     circular     21-FEB-2019
DEFINITION  .
ACCESSION   <unknown id>                                                       
VERSION     <unknown id>                                                       
FEATURES             Location/Qualifiers
     source          2167..2172
                     /label="source:pcDNA3.1(+)"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    667..1383
                     /label="FL GFP"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    1483..1572
                     /label="3CC-Hex2"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     CDS             complement(2396..3055)
                     /label="CamR"
                     /ApEinfo_revcolor=#0000ff
                     /ApEinfo_fwdcolor=#0000ff
     misc_feature    2167..2172
                     /label="XbaI"
                     /ApEinfo_revcolor=#ff0000
                     /ApEinfo_fwdcolor=#ff0000
     protein_bind    complement(2279..2284)
                     /label="BsaI"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    631..660
                     /label="linker"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    619..624
                     /label="GGGGS linker 2"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    595..618
                     /label="FHA binding phos-peptide (AKAR2)"
                     /ApEinfo_revcolor=#587bff
                     /ApEinfo_fwdcolor=#587bff
     misc_feature    2173..2271
                     /label="6_AntiTetramer2-WD"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    complement(2287..2395)
                     /label="CamR Terminator"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    1645..2054
                     /label="FHA1"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    580..594
                     /label="GGGGS linker"
                     /ApEinfo_revcolor=#8000ff
                     /ApEinfo_fwdcolor=#8000ff
     misc_feature    1384..1389
                     /label="BamHI"
                     /ApEinfo_revcolor=#ff0000
                     /ApEinfo_fwdcolor=#ff0000
     misc_feature    2056..2073
                     /label="FHA1"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    1633..1638
                     /label="HindIII"
                     /ApEinfo_revcolor=#ff0000
                     /ApEinfo_fwdcolor=#ff0000
     source          1477..1482
                     /label="source:pcDNA3.1(+)"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     rep_origin      complement(join(3161..3360,1..564))
                     /label="ColE1"
                     /ApEinfo_revcolor=#7f7f7f
                     /ApEinfo_fwdcolor=#7f7f7f
     source          1633..1638
                     /label="source:pcDNA3.1(+)"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    1579..1632
                     /label="T2A"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    1477..1482
                     /label="XbaI"
                     /ApEinfo_revcolor=#ff0000
                     /ApEinfo_fwdcolor=#ff0000
     CDS             667..1383
                     /label="GFP"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     protein_bind    569..574
                     /label="BsaI"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     promoter        complement(3056..3160)
                     /label="CamR Promoter"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    2074..2079
                     /label="BamHI"
                     /ApEinfo_revcolor=#ff0000
                     /ApEinfo_fwdcolor=#ff0000
     misc_feature    661..666
                     /label="ECORV"
                     /ApEinfo_revcolor=#ff0000
                     /ApEinfo_fwdcolor=#ff0000
ORIGIN
        1 agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag
       61 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
      121 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
      181 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
      241 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
      301 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
      361 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
      421 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
      481 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
      541 tcccctgatt ctgtggataa ccgtagtcgg tctcatatgg gcggtggagg gagcCTGCGT
      601 CGCGCCACCC TGGTGGACgg cggaCTCGAG TCCGGACTCA GATCTGGCAG CGGTGGAGGC
      661 GATATCatgg tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag
      721 ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc
      781 acctacggca agctgaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg
      841 cccaccctcg tgaccaccct gacctacggc gtgcagtgct tcagccgcta ccccgaccac
      901 atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc
      961 atcttcttca aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac
     1021 accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg
     1081 gggcacaagc tggagtacaa ctacaacagc cacaacgtct atatcatggc cgacaagcag
     1141 aagaacggca tcaaggccaa cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag
     1201 ctcgccgacc actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac
     1261 aaccactacc tgagcaccca gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac
     1321 atggtcctgc tggagttcgt gaccgccgcc gggatcactc tcggcatgga cgagctgtac
     1381 aagGGATCCG GCAGTGCTGG TGGTAGTGCT GGTGGTAGTG CTGGTGGTAG TGCTGGTGGC
     1441 AGTGCTGGTG GTAGTGCTGG TGGTAGTGCT GGTGGCTCTA GAGGAGAAAT CGCAAAGAGT
     1501 CTGAAAGAAA TTGCAAAATC CTTGAAGGAA ATAGCGTGGT CACTGAAAGA AATTGCCAAG
     1561 TCCCTCAAAG GGGGTACCGA GGGCAGAGGA AGTCTGCTAA CATGCGGTGA CGTCGAGGAG
     1621 AATCCTGGCC CAAAGCTTGC CACCATGCAT AAGTTTTCTC AAGAACAGAT CGGCGAAAAC
     1681 ATTGTGTGCA GGGTCATTTG TACCACGGGT CAAATTCCCA TCCGAGATTT GTCAGCTGAT
     1741 ATTTCACAAG TGCTTAAGGA AAAACGATCC ATAAAGAAAG TTTGGACATT TGGTAGAAAC
     1801 CCAGCCTGTG ACTATCATTT AGGAAACATT TCAAGACTGT CAAATAAGCA TTTCCAAATA
     1861 CTACTAGGAG AAGACGGTAA CCTTTTATTG AATGACATTT CCACTAATGG GACCTGGTTA
     1921 AATGGGCAAA AAGTCGAGAA GAACAGCAAT CAGTTACTGT CTCAAGGTGA TGAAATAACC
     1981 GTTGGTGTAG GCGTGGAATC AGATATTTTA TCTCTGGTCA TTTTCATAAA CGACAAATTT
     2041 AAGCAGTGCC TCGAACAGAA CAAAGTTGAT CGCGGATCCG GCAGTGCTGG TGGTAGTGCT
     2101 GGTGGTAGTG CTGGTGGTAG TGCTGGTGGC AGTGCTGGTG GTAGTGCTGG TGGTAGTGCT
     2161 GGTGGCTCTA GAACCTTGAG AGAAATCGAG GAATTGTTGA GGAAAATCAT TGAAGACAGC
     2221 GTGCGCAGTG TGGCCGAGCT GGAGGACATT GAGAAGTGGC TGAAGAAAAT CGGATCCtga
     2281 gaccagacca ataaaaaacg cccggcggca accgagcgtt ctgaacaaat ccagatggag
     2341 ttctgaggtc attactggat ctatcaacag gagtccaagc gagctcgata tcaaattacg
     2401 ccccgccctg ccactcatcg cagtactgtt gtaattcatt aagcattctg ccgacatgga
     2461 agccatcaca aacggcatga tgaacctgaa tcgccagcgg catcagcacc ttgtcgcctt
     2521 gcgtataata tttgcccatg gtgaaaacgg gggcgaagaa gttgtccata ttggccacgt
     2581 ttaaatcaaa actggtgaaa ctcacccagg gattggctga aacgaaaaac atattctcaa
     2641 taaacccttt agggaaatag gccaggtttt caccgtaaca cgccacatct tgcgaatata
     2701 tgtgtagaaa ctgccggaaa tcgtcgtggt attcactcca gagcgatgaa aacgtttcag
     2761 tttgctcatg gaaaacggtg taacaagggt gaacactatc ccatatcacc agctcaccgt
     2821 ctttcattgc catacgaaat tccggatgag cattcatcag gcgggcaaga atgtgaataa
     2881 aggccggata aaacttgtgc ttatttttct ttacggtctt taaaaaggcc gtaatatcca
     2941 gctgaacggt ctggttatag gtacattgag caactgactg aaatgcctca aaatgttctt
     3001 tacgatgcca ttgggatata tcaacggtgg tatatccagt gatttttttc tccattttag
     3061 cttccttagc tcctgaaaat ctcgataact caaaaaatac gcccggtagt gatcttattt
     3121 cattatggtg aaagttggaa cctcttacgt gcccgatcaa tcatgaccaa aatcccttaa
     3181 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     3241 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     3301 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
//