LOCUS       MTK3a_015               3234 bp ds-DNA     circular     21-FEB-2019
DEFINITION  .
ACCESSION   <unknown id>                                                       
VERSION     <unknown id>                                                       
FEATURES             Location/Qualifiers
     misc_feature    complement(2340..2448)
                     /label="CamR Terminator"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     rep_origin      complement(join(3214..3234,1..743))
                     /label="ColE1"
                     /ApEinfo_revcolor=#7f7f7f
                     /ApEinfo_fwdcolor=#7f7f7f
     CDS             759..908
                     /label="VP64"
                     /ApEinfo_revcolor=#993366
                     /ApEinfo_fwdcolor=#993366
     CDS             915..935
                     /label="SV40 NLS"
                     /ApEinfo_revcolor=#993366
                     /ApEinfo_fwdcolor=#993366
     CDS             1755..2324
                     /label="Rta AD"
                     /ApEinfo_revcolor=#993366
                     /ApEinfo_fwdcolor=#993366
     protein_bind    complement(2332..2337)
                     /label="BsaI"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     protein_bind    748..753
                     /label="BsaI"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     promoter        complement(3109..3213)
                     /label="CamR Promoter"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     CDS             1365..1736
                     /label="RelA (p65) AD"
                     /ApEinfo_revcolor=#993366
                     /ApEinfo_fwdcolor=#993366
     CDS             complement(2449..3108)
                     /label="CamR"
                     /ApEinfo_revcolor=#0000ff
                     /ApEinfo_fwdcolor=#0000ff
ORIGIN
        1 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
       61 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
      121 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
      181 gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga
      241 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
      301 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
      361 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
      421 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
      481 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
      541 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
      601 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
      661 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
      721 cccctgattc tgtggataac cgtagtcggt ctcaTATGga tgctttagac gattttgact
      781 tagatatgct tggttcagac gcgttagacg acttcgacct agacatgtta ggctcagatg
      841 cattggacga cttcgattta gatatgttgg gctccgatgc cctagatgac tttgatctag
      901 atatgctagg tagtcccaaa aagaagagga aagtgggatc ccagtatctg cccgacacag
      961 atgatagaca ccgaatcgaa gagaaacgca agcgaacgta tgaaaccttc aaatcgatca
     1021 tgaagaaatc gcccttctcg ggtccgaccg atcccaggcc cccaccgaga aggattgcgg
     1081 tcccgtcccg ctcgtcggcc agcgtgccga agcctgcgcc gcagccctac cccttcacgt
     1141 cgagcctgag cacaatcaat tatgacgagt tcccgacgat ggtgttcccc tcgggacaaa
     1201 tctcacaagc ctcggcgctc gcaccagcgc ctccccaagt ccttccgcaa gcgcctgccc
     1261 cagcgcctgc accggcaatg gtgtccgccc tcgcacaggc ccctgcgccc gtccccgtgc
     1321 tcgcgcctgg accgccccag gcggtcgctc caccggctcc gaagccgacg caggccggag
     1381 agggaacact ctccgaagca cttcttcaac tccagtttga tgacgaggat cttggagcac
     1441 tccttggaaa ctcgacagac cctgcggtgt ttaccgacct cgcgtcagta gataactccg
     1501 aatttcagca gcttttgaac cagggtatcc cggtcgcgcc acatacaacg gagcccatgt
     1561 tgatggaata ccccgaagca atcacgagac ttgtgacggg agcgcagcgg cctcccgatc
     1621 ccgcacccgc acctttgggg gcacctggcc tccctaacgg acttttgagc ggcgacgagg
     1681 atttctcctc catcgccgat atggatttct cagccttgct gtcacagatt tccagcggct
     1741 ctggcagcgg cagccgggat tccagggaag ggatgttttt gccgaagcct gaggccggct
     1801 ccgctattag tgacgtgttt gagggccgcg aggtgtgcca gccaaaacga atccggccat
     1861 ttcatcctcc aggaagtcca tgggccaacc gcccactccc cgccagcctc gcaccaacac
     1921 caaccggtcc agtacatgag ccagtcgggt cactgacccc ggcaccagtc cctcagccac
     1981 tggatccagc gcccgcagtg actcccgagg ccagtcacct gttggaggat cccgatgaag
     2041 aaacgagcca ggctgtcaaa gcccttcggg agatggccga tactgtgatt ccccagaagg
     2101 aagaggctgc aatctgtggc caaatggacc tttcccatcc gcccccaagg ggccatctgg
     2161 atgagctgac aaccacactt gagtccatga ccgaggatct gaacctggac tcacccctga
     2221 ccccggaatt gaacgagatt ctggatacct tcctgaacga cgagtgcctc ttgcatgcca
     2281 tgcatatcag cacaggactg tccatcttcg acacatctct gtttGGTTCT tgagaccaga
     2341 ccaataaaaa acgcccggcg gcaaccgagc gttctgaaca aatccagatg gagttctgag
     2401 gtcattactg gatctatcaa caggagtcca agcgagctcg atatcaaatt acgccccgcc
     2461 ctgccactca tcgcagtact gttgtaattc attaagcatt ctgccgacat ggaagccatc
     2521 acaaacggca tgatgaacct gaatcgccag cggcatcagc accttgtcgc cttgcgtata
     2581 atatttgccc atggtgaaaa cgggggcgaa gaagttgtcc atattggcca cgtttaaatc
     2641 aaaactggtg aaactcaccc agggattggc tgaaacgaaa aacatattct caataaaccc
     2701 tttagggaaa taggccaggt tttcaccgta acacgccaca tcttgcgaat atatgtgtag
     2761 aaactgccgg aaatcgtcgt ggtattcact ccagagcgat gaaaacgttt cagtttgctc
     2821 atggaaaacg gtgtaacaag ggtgaacact atcccatatc accagctcac cgtctttcat
     2881 tgccatacga aattccggat gagcattcat caggcgggca agaatgtgaa taaaggccgg
     2941 ataaaacttg tgcttatttt tctttacggt ctttaaaaag gccgtaatat ccagctgaac
     3001 ggtctggtta taggtacatt gagcaactga ctgaaatgcc tcaaaatgtt ctttacgatg
     3061 ccattgggat atatcaacgg tggtatatcc agtgattttt ttctccattt tagcttcctt
     3121 agctcctgaa aatctcgata actcaaaaaa tacgcccggt agtgatctta tttcattatg
     3181 gtgaaagttg gaacctctta cgtgcccgat caatcatgac caaaatccct taac
//