LOCUS       MTK3b_003               1731 bp ds-DNA     circular     22-FEB-2019
DEFINITION  .
ACCESSION   <unknown id>                                                       
VERSION     <unknown id>                                                       
KEYWORDS    "mRuby2:CDS-3b"                                                    
FEATURES             Location/Qualifiers
     protein_bind    9..12
                     /label="BsaI"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     promoter        complement(860..964)
                     /label="CamR Promoter"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     terminator      complement(91..199)
                     /label="CamR Terminator"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     CDS             complement(200..859)
                     /label="CamR"
                     /ApEinfo_revcolor=#0000ff
                     /ApEinfo_fwdcolor=#0000ff
     protein_bind    complement(83..88)
                     /label="BsaI"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     rep_origin      complement(965..1728)
                     /label="ColE1"
                     /ApEinfo_revcolor=#7f7f7f
                     /ApEinfo_fwdcolor=#7f7f7f
     misc_signal     13..75
                     /label="T2A"
                     /ApEinfo_revcolor=#0080ff
                     /ApEinfo_fwdcolor=#0080ff
     protein_bind    complement(78..81)
                     /label="BsaI"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     protein_bind    2..7
                     /label="BsaI"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
ORIGIN
        1 cggtctcatt ctGGAAGCGG AGAGGGCAGA GGAAGTCTGC TAACATGCGG TGACGTCGAG
       61 GAGAATCCTG GACCTggatc ctgagaccag accaataaaa aacgcccggc ggcaaccgag
      121 cgttctgaac aaatccagat ggagttctga ggtcattact ggatctatca acaggagtcc
      181 aagcgagctc gatatcaaat tacgccccgc cctgccactc atcgcagtac tgttgtaatt
      241 cattaagcat tctgccgaca tggaagccat cacaaacggc atgatgaacc tgaatcgcca
      301 gcggcatcag caccttgtcg ccttgcgtat aatatttgcc catggtgaaa acgggggcga
      361 agaagttgtc catattggcc acgtttaaat caaaactggt gaaactcacc cagggattgg
      421 ctgaaacgaa aaacatattc tcaataaacc ctttagggaa ataggccagg ttttcaccgt
      481 aacacgccac atcttgcgaa tatatgtgta gaaactgccg gaaatcgtcg tggtattcac
      541 tccagagcga tgaaaacgtt tcagtttgct catggaaaac ggtgtaacaa gggtgaacac
      601 tatcccatat caccagctca ccgtctttca ttgccatacg aaattccgga tgagcattca
      661 tcaggcgggc aagaatgtga ataaaggccg gataaaactt gtgcttattt ttctttacgg
      721 tctttaaaaa ggccgtaata tccagctgaa cggtctggtt ataggtacat tgagcaactg
      781 actgaaatgc ctcaaaatgt tctttacgat gccattggga tatatcaacg gtggtatatc
      841 cagtgatttt tttctccatt ttagcttcct tagctcctga aaatctcgat aactcaaaaa
      901 atacgcccgg tagtgatctt atttcattat ggtgaaagtt ggaacctctt acgtgcccga
      961 tcaatcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
     1021 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
     1081 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
     1141 tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag
     1201 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
     1261 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
     1321 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     1381 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
     1441 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     1501 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
     1561 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
     1621 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
     1681 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtag t
//