LOCUS       MTK3b_010               2439 bp ds-DNA     circular     22-FEB-2019
DEFINITION  .
ACCESSION   <unknown id>                                                       
VERSION     <unknown id>                                                       
FEATURES             Location/Qualifiers
     misc_feature    10..13
                     /label="BsaI"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    complement(1569..1673)
                     /label="CamR Promoter"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    complement(792..797)
                     /label="BsaI"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    complement(241..260)
                     /label="mScarlet sgRNA1"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     misc_feature    complement(909..1568)
                     /label="CamR"
                     /ApEinfo_revcolor=#0000ff
                     /ApEinfo_fwdcolor=#0000ff
     misc_feature    3..8
                     /label="BsaI"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    complement(787..790)
                     /label="BsaI"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    complement(1674..2437)
                     /label="ColE1"
                     /ApEinfo_revcolor=#7f7f7f
                     /ApEinfo_fwdcolor=#7f7f7f
     misc_feature    complement(800..908)
                     /label="CamR Terminator"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
ORIGIN
        1 tcggtctcat tctgtatcaa aaggcgaggc ggtcattaaa gagttcatga ggtttaaagt
       61 ccatatggaa ggaagcatga atggtcacga gtttgagata gagggagagg gtgagggaag
      121 gccgtacgaa gggacgcaaa ctgcgaagtt gaaggtaacg aaaggcggcc ctctgccatt
      181 ctcttgggac atcctttcac cgcaatttat gtacggctct agggccttca caaaacaccc
      241 cgccgatatt ccggattact ataaacaaag ttttcctgag ggcttcaagt gggaaagagt
      301 aatgaacttc gaggatgggg gagctgtgac ggttactcaa gacactagcc tggaggacgg
      361 tactcttata tacaaggtaa aactaagagg gacgaatttt ccgccagacg gccccgtgat
      421 gcaaaaaaaa actatggggt gggaagcatc caccgagagg ctgtaccccg aagacggggt
      481 cctgaagggt gatattaaaa tggcattaag attaaaagat ggcgggagat acttagctga
      541 cttcaagact acatataaag ccaaaaaacc tgtacagatg cctggagctt acaatgtgga
      601 tcgtaagctg gatattacat cccacaacga agattatacc gtggtggagc agtacgagcg
      661 ttcagagggg cgtcactcca ccgggggtat ggatgaatta tacaagagcg gtcttagaag
      721 cagggcgcaa gcgtcaaatt cagcggtaga tggaacagca ggtccaggta gtactggcag
      781 cagaggatcc tgagaccaga ccaataaaaa acgcccggcg gcaaccgagc gttctgaaca
      841 aatccagatg gagttctgag gtcattactg gatctatcaa caggagtcca agcgagctcg
      901 atatcaaatt acgccccgcc ctgccactca tcgcagtact gttgtaattc attaagcatt
      961 ctgccgacat ggaagccatc acaaacggca tgatgaacct gaatcgccag cggcatcagc
     1021 accttgtcgc cttgcgtata atatttgccc atggtgaaaa cgggggcgaa gaagttgtcc
     1081 atattggcca cgtttaaatc aaaactggtg aaactcaccc agggattggc tgaaacgaaa
     1141 aacatattct caataaaccc tttagggaaa taggccaggt tttcaccgta acacgccaca
     1201 tcttgcgaat atatgtgtag aaactgccgg aaatcgtcgt ggtattcact ccagagcgat
     1261 gaaaacgttt cagtttgctc atggaaaacg gtgtaacaag ggtgaacact atcccatatc
     1321 accagctcac cgtctttcat tgccatacga aattccggat gagcattcat caggcgggca
     1381 agaatgtgaa taaaggccgg ataaaacttg tgcttatttt tctttacggt ctttaaaaag
     1441 gccgtaatat ccagctgaac ggtctggtta taggtacatt gagcaactga ctgaaatgcc
     1501 tcaaaatgtt ctttacgatg ccattgggat atatcaacgg tggtatatcc agtgattttt
     1561 ttctccattt tagcttcctt agctcctgaa aatctcgata actcaaaaaa tacgcccggt
     1621 agtgatctta tttcattatg gtgaaagttg gaacctctta cgtgcccgat caatcatgac
     1681 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     1741 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     1801 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     1861 aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg
     1921 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     1981 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     2041 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     2101 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
     2161 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     2221 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     2281 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     2341 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
     2401 ctttcctgcg ttatcccctg attctgtgga taaccgtag
//