LOCUS       MTK3b_012               2691 bp ds-DNA     circular     22-FEB-2019
DEFINITION  .
ACCESSION   <unknown id>                                                       
VERSION     <unknown id>                                                       
FEATURES             Location/Qualifiers
     misc_feature    complement(1797..1905)
                     /label="CamR Terminator"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     CDS             759..1781
                     /label="HPH (Hygromycin Phosphotransferase)"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     promoter        complement(2566..2670)
                     /label="CamR Promoter"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     rep_origin      complement(join(2671..2691,1..743))
                     /label="ColE1"
                     /ApEinfo_revcolor=#7f7f7f
                     /ApEinfo_fwdcolor=#7f7f7f
     CDS             complement(1906..2565)
                     /label="CamR"
                     /ApEinfo_revcolor=#0000ff
                     /ApEinfo_fwdcolor=#0000ff
     protein_bind    748..753
                     /label="BsaI"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     protein_bind    complement(1789..1794)
                     /label="BsaI"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
ORIGIN
        1 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
       61 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
      121 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
      181 gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga
      241 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
      301 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
      361 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
      421 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
      481 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
      541 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
      601 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
      661 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
      721 cccctgattc tgtggataac cgtagtcggt ctcaTTCTgg taaaaagcct gaactcaccg
      781 cgacgtctgt cgagaagttt ctgatcgaaa agttcgacag cgtgtccgac ctgatgcagc
      841 tctcggaggg cgaagaatct cgtgctttca gcttcgatgt aggagggcgt ggatatgtcc
      901 tgcgggtaaa tagctgcgcc gatggtttct acaaagatcg ttatgtttat cggcactttg
      961 catcggccgc gctcccgatt ccggaagtgc ttgacattgg ggaatttagc gagagcctga
     1021 cctattgcat ctcccgccgt gcacagggtg tcacgttgca agacctgcct gaaaccgaac
     1081 tgcccgctgt tctgcaaccg gtcgcggagg ccatggatgc gatcgctgcg gccgatctta
     1141 gccagacgag cgggttcggc ccattcggac cgcaaggaat cggtcaatac actacatggc
     1201 gtgatttcat atgcgcgatt gctgatcccc atgtgtatca ctggcaaact gtgatggacg
     1261 acaccgtcag tgcgtccgtc gcgcaggctc tcgatgagct gatgctttgg gccgaggact
     1321 gccccgaagt ccggcacctc gtgcacgcgg atttcggctc caacaatgtc ctgacggaca
     1381 atggccgcat aacagcggtc attgactgga gcgaggcgat gttcggggat tcccaatacg
     1441 aggtcgccaa catcttcttc tggaggccgt ggttggcttg tatggagcag cagacgcgct
     1501 acttcgagcg gaggcatccg gagcttgcag gatcgccgcg gctccgggcg tatatgctcc
     1561 gcattggtct tgaccaactc tatcagagct tggttgacgg caatttcgat gatgcagctt
     1621 gggcgcaggg tcgatgcgac gcaatcgtcc gatccggagc cgggactgtc gggcgtacac
     1681 aaatcgcccg cagaagcgcg gccgtctgga ccgatggctg tgtagaagta ctcgccgata
     1741 gtggaaaccg acgccccagc actcgtccga gggcaaagga aGGATCCtga gaccagacca
     1801 ataaaaaacg cccggcggca accgagcgtt ctgaacaaat ccagatggag ttctgaggtc
     1861 attactggat ctatcaacag gagtccaagc gagctcgata tcaaattacg ccccgccctg
     1921 ccactcatcg cagtactgtt gtaattcatt aagcattctg ccgacatgga agccatcaca
     1981 aacggcatga tgaacctgaa tcgccagcgg catcagcacc ttgtcgcctt gcgtataata
     2041 tttgcccatg gtgaaaacgg gggcgaagaa gttgtccata ttggccacgt ttaaatcaaa
     2101 actggtgaaa ctcacccagg gattggctga aacgaaaaac atattctcaa taaacccttt
     2161 agggaaatag gccaggtttt caccgtaaca cgccacatct tgcgaatata tgtgtagaaa
     2221 ctgccggaaa tcgtcgtggt attcactcca gagcgatgaa aacgtttcag tttgctcatg
     2281 gaaaacggtg taacaagggt gaacactatc ccatatcacc agctcaccgt ctttcattgc
     2341 catacgaaat tccggatgag cattcatcag gcgggcaaga atgtgaataa aggccggata
     2401 aaacttgtgc ttatttttct ttacggtctt taaaaaggcc gtaatatcca gctgaacggt
     2461 ctggttatag gtacattgag caactgactg aaatgcctca aaatgttctt tacgatgcca
     2521 ttgggatata tcaacggtgg tatatccagt gatttttttc tccattttag cttccttagc
     2581 tcctgaaaat ctcgataact caaaaaatac gcccggtagt gatcttattt cattatggtg
     2641 aaagttggaa cctcttacgt gcccgatcaa tcatgaccaa aatcccttaa c
//