LOCUS       MTK3b_016               2493 bp ds-DNA     circular     22-FEB-2019
DEFINITION  .
ACCESSION   <unknown id>                                                       
VERSION     <unknown id>                                                       
KEYWORDS    "mRuby2:CDS-3b"                                                    
FEATURES             Location/Qualifiers
     rep_origin      complement(4..767)
                     /label="ColE1"
                     /ApEinfo_revcolor=#7f7f7f
                     /ApEinfo_fwdcolor=#7f7f7f
     protein_bind    779..782
                     /label="BsaI"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     CDS             complement(1732..2391)
                     /label="CamR"
                     /ApEinfo_revcolor=#0000ff
                     /ApEinfo_fwdcolor=#0000ff
     protein_bind    complement(1615..1620)
                     /label="BsaI"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     protein_bind    complement(1610..1613)
                     /label="BsaI"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     promoter        complement(join(2392..2493,1..3))
                     /label="CamR Promoter"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     protein_bind    772..777
                     /label="BsaI"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    783..803
                     /label="linker"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     terminator      complement(1623..1731)
                     /label="CamR Terminator"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    804..1583
                     /label="Cx30 (human)"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    1584..1607
                     /label="linker"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
ORIGIN
        1 caatcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
       61 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
      121 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
      181 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
      241 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
      301 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
      361 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
      421 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
      481 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
      541 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
      601 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
      661 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
      721 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtagt cggtctcatt
      781 ctggactccg ctctcgtgct cagGATTGGG GGACGCTGCA CACTTTCATC GGGGGTGTCA
      841 ACAAACACTC CACCAGCATC GGGAAGGTGT GGATCACAGT CATCTTTATT TTCCGAGTCA
      901 TGATCCTCGT GGTGGCTGCC CAGGAAGTGT GGGGTGACGA GCAAGAGGAC TTCGTCTGCA
      961 ACACACTGCA ACCGGGATGC AAAAATGTGT GCTATGACCA CTTTTTCCCG GTGTCCCACA
     1021 TCCGGCTGTG GGCCCTCCAG CTGATCTTtG TCTCCACCCC AGCGCTGCTG GTGGCCATGC
     1081 ATGTGGCCTA CTACAGGCAC GAAACCACTC GCAAGTTCAG GCGAGGAGAG AAGAGGAATG
     1141 ATTTCAAAGA CATAGAGGAC ATTAAAAAGC AGAAGGTTCG GATAGAGGGG TCGCTGTGGT
     1201 GGACGTACAC CAGCAGCATC TTTTTCCGAA TCATCTTTGA AGCAGCCTTT ATGTATGTGT
     1261 TTTACTTCCT TTACAATGGG TACCACCTGC CCTGGGTGTT GAAATGTGGG ATTGACCCCT
     1321 GCCCCAACCT TGTTGACTGC TTTATTTCTA GGCCAACAGA GAAGACCGTG TTTACCATTT
     1381 TTATGATTTC TGCGTCTGTG ATTTGCATGC TGCTTAACGT GGCAGAGTTG TGCTACCTGC
     1441 TGCTGAAAGT GTGTTTTAGG AGATCAAAGA GAGCACAGAC GCAAAAAAAT CACCCCAATC
     1501 ATGCCCTAAA GGAGAGTAAG CAGAATGAAA TGAATGAGCT GATTTCAGAT AGTGGTCAAA
     1561 ATGCAATCAC AGGTTTCCCA AGCTACCCGG ATCCACCGGT CGCCACCgga tcctgagacc
     1621 agaccaataa aaaacgcccg gcggcaaccg agcgttctga acaaatccag atggagttct
     1681 gaggtcatta ctggatctat caacaggagt ccaagcgagc tcgatatcaa attacgcccc
     1741 gccctgccac tcatcgcagt actgttgtaa ttcattaagc attctgccga catggaagcc
     1801 atcacaaacg gcatgatgaa cctgaatcgc cagcggcatc agcaccttgt cgccttgcgt
     1861 ataatatttg cccatggtga aaacgggggc gaagaagttg tccatattgg ccacgtttaa
     1921 atcaaaactg gtgaaactca cccagggatt ggctgaaacg aaaaacatat tctcaataaa
     1981 ccctttaggg aaataggcca ggttttcacc gtaacacgcc acatcttgcg aatatatgtg
     2041 tagaaactgc cggaaatcgt cgtggtattc actccagagc gatgaaaacg tttcagtttg
     2101 ctcatggaaa acggtgtaac aagggtgaac actatcccat atcaccagct caccgtcttt
     2161 cattgccata cgaaattccg gatgagcatt catcaggcgg gcaagaatgt gaataaaggc
     2221 cggataaaac ttgtgcttat ttttctttac ggtctttaaa aaggccgtaa tatccagctg
     2281 aacggtctgg ttataggtac attgagcaac tgactgaaat gcctcaaaat gttctttacg
     2341 atgccattgg gatatatcaa cggtggtata tccagtgatt tttttctcca ttttagcttc
     2401 cttagctcct gaaaatctcg ataactcaaa aaatacgccc ggtagtgatc ttatttcatt
     2461 atggtgaaag ttggaacctc ttacgtgccc gat
//