LOCUS       MTK3b_022               1905 bp ds-DNA     circular     22-FEB-2019
DEFINITION  .
ACCESSION   <unknown id>                                                       
VERSION     <unknown id>                                                       
KEYWORDS    "mRuby2:CDS-3b"                                                    
FEATURES             Location/Qualifiers
     protein_bind    complement(1078..1083)
                     /label="BsaI"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     promoter        complement(join(1855..1905,1..54))
                     /label="CamR Promoter"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    1047..1070
                     /label="krab"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     protein_bind    823..828
                     /label="BsaI"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     protein_bind    complement(1073..1076)
                     /label="BsaI"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    834..1046
                     /label="krab"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     terminator      complement(1086..1194)
                     /label="CamR Terminator"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     protein_bind    830..833
                     /label="BsaI"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     CDS             complement(1195..1854)
                     /label="CamR"
                     /ApEinfo_revcolor=#0000ff
                     /ApEinfo_fwdcolor=#0000ff
     rep_origin      complement(55..818)
                     /label="ColE1"
                     /ApEinfo_revcolor=#7f7f7f
                     /ApEinfo_fwdcolor=#7f7f7f
ORIGIN
        1 tagtgatctt atttcattat ggtgaaagtt ggaacctctt acgtgcccga tcaatcatga
       61 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
      121 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
      181 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
      241 taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag
      301 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
      361 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
      421 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
      481 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
      541 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
      601 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
      661 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
      721 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
      781 tctttcctgc gttatcccct gattctgtgg ataaccgtag tcggtctcat tctGATGCTA
      841 AGTCACTAAC TGCCTGGTCC CGGACACTGG TGACCTTCAA GGATGTATTT GTGGACTTCA
      901 CCAGGGAGGA GTGGAAGCTG CTGGACACTG CTCAGCAGAT CGTGTACAGA AATGTGATGC
      961 TGGAGAACTA TAAGAACCTG GTTTCCTTGG GTTATCAGCT TACTAAGCCA GATGTGATCC
     1021 TCCGGTTGGA GAAGGGAGAA GAGCCCGGCG GTTCCGGCGG AGGGTCGATG GGatcctgag
     1081 accagaccaa taaaaaacgc ccggcggcaa ccgagcgttc tgaacaaatc cagatggagt
     1141 tctgaggtca ttactggatc tatcaacagg agtccaagcg agctcgatat caaattacgc
     1201 cccgccctgc cactcatcgc agtactgttg taattcatta agcattctgc cgacatggaa
     1261 gccatcacaa acggcatgat gaacctgaat cgccagcggc atcagcacct tgtcgccttg
     1321 cgtataatat ttgcccatgg tgaaaacggg ggcgaagaag ttgtccatat tggccacgtt
     1381 taaatcaaaa ctggtgaaac tcacccaggg attggctgaa acgaaaaaca tattctcaat
     1441 aaacccttta gggaaatagg ccaggttttc accgtaacac gccacatctt gcgaatatat
     1501 gtgtagaaac tgccggaaat cgtcgtggta ttcactccag agcgatgaaa acgtttcagt
     1561 ttgctcatgg aaaacggtgt aacaagggtg aacactatcc catatcacca gctcaccgtc
     1621 tttcattgcc atacgaaatt ccggatgagc attcatcagg cgggcaagaa tgtgaataaa
     1681 ggccggataa aacttgtgct tatttttctt tacggtcttt aaaaaggccg taatatccag
     1741 ctgaacggtc tggttatagg tacattgagc aactgactga aatgcctcaa aatgttcttt
     1801 acgatgccat tgggatatat caacggtggt atatccagtg atttttttct ccattttagc
     1861 ttccttagct cctgaaaatc tcgataactc aaaaaatacg cccgg
//