LOCUS       MTK3b_027               2844 bp ds-DNA     circular     22-FEB-2019
DEFINITION  .
ACCESSION   <unknown id>                                                       
VERSION     <unknown id>                                                       
KEYWORDS    "mRuby2:CDS-3b"                                                    
FEATURES             Location/Qualifiers
     protein_bind    823..828
                     /label="BsaI"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     protein_bind    complement(2012..2015)
                     /label="BsaI"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     promoter        complement(join(2794..2844,1..54))
                     /label="CamR Promoter"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     exon            1613..1749
                     /label="TP53-001 Exon 8"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     exon            1750..1823
                     /label="TP53-001 Exon 9"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     terminator      complement(2025..2133)
                     /label="CamR Terminator"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     exon            1503..1612
                     /label="TP53-001 Exon 7"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     exon            1824..1930
                     /label="TP53-001 Exon 10"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     exon            905..926
                     /label="TP53-001 Exon 3"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     exon            1390..1502
                     /label="TP53-001 Exon 6"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     exon            927..1205
                     /label="TP53-001 Exon 4"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     protein_bind    830..833
                     /label="BsaI"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     exon            1206..1389
                     /label="TP53-001 Exon 5"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     protein_bind    complement(2017..2022)
                     /label="BsaI"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     rep_origin      complement(55..818)
                     /label="ColE1"
                     /ApEinfo_revcolor=#7f7f7f
                     /ApEinfo_fwdcolor=#7f7f7f
     CDS             complement(2134..2793)
                     /label="CamR"
                     /ApEinfo_revcolor=#0000ff
                     /ApEinfo_fwdcolor=#0000ff
ORIGIN
        1 tagtgatctt atttcattat ggtgaaagtt ggaacctctt acgtgcccga tcaatcatga
       61 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
      121 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
      181 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
      241 taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag
      301 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
      361 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
      421 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
      481 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
      541 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
      601 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
      661 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
      721 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
      781 tctttcctgc gttatcccct gattctgtgg ataaccgtag tcggtctcat tctGAGGAGC
      841 CGCAGTCAGA TCCTAGCGTC GAGCCCCCTC TGAGTCAGGA AACATTTTCA GACCTATGGA
      901 AACTACTTCC TGAAAACAAC GTTCTGTCCC CCTTGCCGTC CCAAGCAATG GATGATTTGA
      961 TGCTGTCCCC GGACGATATT GAACAATGGT TCACTGAAGA CCCAGGTCCA GATGAAGCTC
     1021 CCAGAATGCC AGAGGCTGCT CCCCCCGTgg cccctgcacc agcagctcct acaccggcgg
     1081 cccctgcacc agcCCCCTCC TGGCCCCTGT CATCTTCTGT CCCTTCCCAG AAAACCTACC
     1141 AGGGCAGCTA CGGTTTCCGT CTGGGCTTCT TGCATTCTGG GACAGCCAAG TCTGTGACTT
     1201 GCACGTACTC CCCTGCCCTC AACAAGATGT TTTGCCAACT GGCCAAGACC TGCCCTGTGC
     1261 AGCTGTGGGT TGATTCCACA CCCCCGCCCG GCACCCGCGT CCGCGCCATG GCCATCTACA
     1321 AGCAGTCACA GCACATGACG GAGGTTGTGA GGCGCTGCCC CCACCATGAG CGCTGCTCAG
     1381 ATAGCGATGG TCTGGCCCCT CCTCAGCATC TTATCCGAGT GGAAGGAAAT TTGCGTGTGG
     1441 AGTATTTGGA TGACAGAAAC ACTTTTCGAC ATAGTGTGGT GGTGCCCTAT GAGCCGCCTG
     1501 AGGTTGGCTC TGACTGTACC ACCATCCACT ACAACTACAT GTGTAACAGT TCCTGCATGG
     1561 GCGGCATGAA CCGGAGGCCC ATCCTCACCA TCATCACACT GGAAGACTCC AGTGGTAATC
     1621 TACTGGGACG GAACAGCTTT GAGGTGCGTG TTTGTGCCTG TCCTGGGAGA GATCGGCGCA
     1681 CAGAGGAAGA GAATCTCCGC AAGAAAGGGG AGCCTCACCA CGAGCTGCCC CCAGGGAGCA
     1741 CTAAGCGAGC ACTGCCCAAC AACACCAGCT CCTCTCCCCA GCCAAAGAAG AAACCACTGG
     1801 ATGGAGAATA TTTCACCCTT CAGATCCGTG GGCGTGAGCG CTTCGAGATG TTCCGAGAGC
     1861 TGAATGAGGC CTTGGAACTC AAGGATGCCC AGGCTGGGAA GGAGCCAGGG GGGAGCAGGG
     1921 CTCACTCCAG CCACCTGAAG TCCAAAAAGG GTCAGTCTAC CTCCCGCCAT AAAAAACTCA
     1981 TGTTCAAGAC AGAAGGGCCT GACTCAGACG Gatcctgaga ccagaccaat aaaaaacgcc
     2041 cggcggcaac cgagcgttct gaacaaatcc agatggagtt ctgaggtcat tactggatct
     2101 atcaacagga gtccaagcga gctcgatatc aaattacgcc ccgccctgcc actcatcgca
     2161 gtactgttgt aattcattaa gcattctgcc gacatggaag ccatcacaaa cggcatgatg
     2221 aacctgaatc gccagcggca tcagcacctt gtcgccttgc gtataatatt tgcccatggt
     2281 gaaaacgggg gcgaagaagt tgtccatatt ggccacgttt aaatcaaaac tggtgaaact
     2341 cacccaggga ttggctgaaa cgaaaaacat attctcaata aaccctttag ggaaataggc
     2401 caggttttca ccgtaacacg ccacatcttg cgaatatatg tgtagaaact gccggaaatc
     2461 gtcgtggtat tcactccaga gcgatgaaaa cgtttcagtt tgctcatgga aaacggtgta
     2521 acaagggtga acactatccc atatcaccag ctcaccgtct ttcattgcca tacgaaattc
     2581 cggatgagca ttcatcaggc gggcaagaat gtgaataaag gccggataaa acttgtgctt
     2641 atttttcttt acggtcttta aaaaggccgt aatatccagc tgaacggtct ggttataggt
     2701 acattgagca actgactgaa atgcctcaaa atgttcttta cgatgccatt gggatatatc
     2761 aacggtggta tatccagtga tttttttctc cattttagct tccttagctc ctgaaaatct
     2821 cgataactca aaaaatacgc ccgg
//