LOCUS       MTK3b_028               2379 bp ds-DNA     circular     22-FEB-2019
DEFINITION  .
ACCESSION   <unknown id>                                                       
VERSION     <unknown id>                                                       
FEATURES             Location/Qualifiers
     protein_bind    complement(732..737)
                     /label="BsaI"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     promoter        complement(1509..1613)
                     /label="CamR Promoter"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     protein_bind    10..13
                     /label="BsaI"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     CDS             14..724
                     /label="Venus"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     protein_bind    3..8
                     /label="BsaI"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     terminator      complement(740..848)
                     /label="CamR Terminator"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     CDS             complement(849..1508)
                     /label="CamR"
                     /ApEinfo_revcolor=#0000ff
                     /ApEinfo_fwdcolor=#0000ff
     rep_origin      complement(1614..2377)
                     /label="ColE1"
                     /ApEinfo_revcolor=#7f7f7f
                     /ApEinfo_fwdcolor=#7f7f7f
     protein_bind    complement(727..730)
                     /label="BsaI"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
ORIGIN
        1 tcggtctcat tcttctaaag gtgaagaatt attcactggt gttgtcccaa ttttggttga
       61 attagatggt gatgttaatg gtcacaaatt ttctgtctcc ggtgaaggtg aaggtgatgc
      121 tacttacggt aaattgacct taaaattgat ttgtactact ggtaaattgc cagttccatg
      181 gccaacctta gtcactactt taggttatgg tttgcaatgt tttgctagat acccagatca
      241 tatgaaacaa catgactttt tcaagtctgc catgccagaa ggttatgttc aagaaagaac
      301 tatttttttc aaagatgacg gtaactacaa gaccagagct gaagtcaagt ttgaaggtga
      361 taccttagtt aatagaatcg aattaaaagg tattgatttt aaagaaggtg gtaacatttt
      421 aggtcacaaa ttggaataca actataactc tcacaatgtt tacatcactg ctgacaaaca
      481 aaagaatggt atcaaagcta acttcaaaat tagacacaac attgaagatg gtggtgttca
      541 attagctgac cattatcaac aaaatactcc aattggtgat ggtccagtct tgttaccaga
      601 caaccattac ttatcctatc aatctgcctt atccaaagat ccaaacgaaa agagagatca
      661 catggtcttg ttagaatttg ttactgctgc tggtattacc catggtatgg atgaattgta
      721 caaaggatcc tgagaccaga ccaataaaaa acgcccggcg gcaaccgagc gttctgaaca
      781 aatccagatg gagttctgag gtcattactg gatctatcaa caggagtcca agcgagctcg
      841 atatcaaatt acgccccgcc ctgccactca tcgcagtact gttgtaattc attaagcatt
      901 ctgccgacat ggaagccatc acaaacggca tgatgaacct gaatcgccag cggcatcagc
      961 accttgtcgc cttgcgtata atatttgccc atggtgaaaa cgggggcgaa gaagttgtcc
     1021 atattggcca cgtttaaatc aaaactggtg aaactcaccc agggattggc tgaaacgaaa
     1081 aacatattct caataaaccc tttagggaaa taggccaggt tttcaccgta acacgccaca
     1141 tcttgcgaat atatgtgtag aaactgccgg aaatcgtcgt ggtattcact ccagagcgat
     1201 gaaaacgttt cagtttgctc atggaaaacg gtgtaacaag ggtgaacact atcccatatc
     1261 accagctcac cgtctttcat tgccatacga aattccggat gagcattcat caggcgggca
     1321 agaatgtgaa taaaggccgg ataaaacttg tgcttatttt tctttacggt ctttaaaaag
     1381 gccgtaatat ccagctgaac ggtctggtta taggtacatt gagcaactga ctgaaatgcc
     1441 tcaaaatgtt ctttacgatg ccattgggat atatcaacgg tggtatatcc agtgattttt
     1501 ttctccattt tagcttcctt agctcctgaa aatctcgata actcaaaaaa tacgcccggt
     1561 agtgatctta tttcattatg gtgaaagttg gaacctctta cgtgcccgat caatcatgac
     1621 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     1681 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     1741 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     1801 aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg
     1861 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     1921 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     1981 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     2041 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
     2101 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     2161 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     2221 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     2281 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
     2341 ctttcctgcg ttatcccctg attctgtgga taaccgtag
//