LOCUS       MTK4_004                1706 bp ds-DNA     circular     22-FEB-2019
DEFINITION  .
ACCESSION   <unknown id>                                                       
VERSION     <unknown id>                                                       
FEATURES             Location/Qualifiers
     rep_origin      complement(join(1352..1706,1..409))
                     /label="ColE1"
                     /ApEinfo_revcolor=#7f7f7f
                     /ApEinfo_fwdcolor=#7f7f7f
     protein_bind    414..419
                     /label="BsaI"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    428..464
                     /label="Spacer"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    complement(478..586)
                     /label="CamR Terminator"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     CDS             complement(587..1246)
                     /label="CamR"
                     /ApEinfo_revcolor=#0000ff
                     /ApEinfo_fwdcolor=#0000ff
     promoter        complement(1247..1351)
                     /label="CamR Promoter"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     protein_bind    complement(470..475)
                     /label="BsaI"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
ORIGIN
        1 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
       61 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
      121 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
      181 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
      241 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
      301 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
      361 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta gtcggtctca
      421 ATCCTAAact agagatctat gtgaggatcc taactcgaga tcgaGCTGtg agaccagacc
      481 aataaaaaac gcccggcggc aaccgagcgt tctgaacaaa tccagatgga gttctgaggt
      541 cattactgga tctatcaaca ggagtccaag cgagctcgat atcaaattac gccccgccct
      601 gccactcatc gcagtactgt tgtaattcat taagcattct gccgacatgg aagccatcac
      661 aaacggcatg atgaacctga atcgccagcg gcatcagcac cttgtcgcct tgcgtataat
      721 atttgcccat ggtgaaaacg ggggcgaaga agttgtccat attggccacg tttaaatcaa
      781 aactggtgaa actcacccag ggattggctg aaacgaaaaa catattctca ataaaccctt
      841 tagggaaata ggccaggttt tcaccgtaac acgccacatc ttgcgaatat atgtgtagaa
      901 actgccggaa atcgtcgtgg tattcactcc agagcgatga aaacgtttca gtttgctcat
      961 ggaaaacggt gtaacaaggg tgaacactat cccatatcac cagctcaccg tctttcattg
     1021 ccatacgaaa ttccggatga gcattcatca ggcgggcaag aatgtgaata aaggccggat
     1081 aaaacttgtg cttatttttc tttacggtct ttaaaaaggc cgtaatatcc agctgaacgg
     1141 tctggttata ggtacattga gcaactgact gaaatgcctc aaaatgttct ttacgatgcc
     1201 attgggatat atcaacggtg gtatatccag tgattttttt ctccatttta gcttccttag
     1261 ctcctgaaaa tctcgataac tcaaaaaata cgcccggtag tgatcttatt tcattatggt
     1321 gaaagttgga acctcttacg tgcccgatca atcatgacca aaatccctta acgtgagttt
     1381 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
     1441 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
     1501 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
     1561 ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta
     1621 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
     1681 aagtcgtgtc ttaccgggtt ggactc
//