LOCUS       MTK4a_010               2488 bp ds-DNA     circular     22-FEB-2019
DEFINITION  .
ACCESSION   <unknown id>                                                       
VERSION     <unknown id>                                                       
FEATURES             Location/Qualifiers
     rep_origin      complement(join(2468..2488,1..743))
                     /label="ColE1"
                     /ApEinfo_revcolor=#7f7f7f
                     /ApEinfo_fwdcolor=#7f7f7f
     misc_signal     759..821
                     /label="T2A"
                     /ApEinfo_revcolor=#0080ff
                     /ApEinfo_fwdcolor=#0080ff
     protein_bind    complement(1586..1591)
                     /label="BsaI"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     sig_peptide     828..857
                     /label="NLS_(zhang)"
                     /ApEinfo_revcolor=#ff0f00
                     /ApEinfo_fwdcolor=#ff0f00
     misc_feature    complement(1594..1702)
                     /label="CamR Terminator"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     protein_bind    748..753
                     /label="BsaI"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     CDS             complement(1703..2362)
                     /label="CamR"
                     /ApEinfo_revcolor=#0000ff
                     /ApEinfo_fwdcolor=#0000ff
     CDS             864..1571
                     /label="mRuby2"
                     /ApEinfo_revcolor=#ff454e
                     /ApEinfo_fwdcolor=#ff454e
     promoter        complement(2363..2467)
                     /label="CamR Promoter"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
ORIGIN
        1 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
       61 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
      121 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
      181 gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga
      241 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
      301 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
      361 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
      421 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
      481 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
      541 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
      601 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
      661 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
      721 cccctgattc tgtggataac cgtagtcggt ctcaATCCGG AAGCGGAGAG GGCAGAGGAA
      781 GTCTGCTAAC ATGCGGTGAC GTCGAGGAGA ATCCTGGACC TGGATCCCCC AAGAAGAAGA
      841 GGAAGGTGGA GGACGCCGGA TCCgtgtcca aaggagagga gttaatcaag gaaaacatga
      901 gaatgaaagt tgtcatggag ggctccgtta atggtcacca attcaagtgt acaggggaag
      961 gtgaaggtaa tccttacatg ggtacacaaa ctatgagaat taaagtaatt gaaggcggac
     1021 cactaccatt tgcatttgac attctggcaa cgtcattcat gtacggatca cgaactttca
     1081 tcaagtaccc taaaggtata ccagactttt tcaagcaatc ttttccagag ggttttacat
     1141 gggaaagggt tacaagatac gaagatgggg gtgtcgtcac agttatgcaa gatacttcat
     1201 tagaagatgg ctgccttgtc tatcatgtgc aagtaagagg ggtgaatttt ccttctaacg
     1261 gacctgtgat gcagaaaaag accaaaggtt gggaaccaaa tactgaaatg atgtacccag
     1321 ctgatggagg tttgagaggc tacacacaca tggcgcttaa agttgatggt ggaggtcatt
     1381 tgtcttgtag ttttgttacc acttatcgtt ctaaaaagac tgttggcaat atcaaaatgc
     1441 caggaataca tgctgtagac cacagactag aaagactcga agagagcgat aacgaaatgt
     1501 tcgttgtaca gagagagcat gccgtagcca aatttgctgg cttaggcggt ggtatggatg
     1561 aattgtataa gTAACTCGAG TGGCtgagac cagaccaata aaaaacgccc ggcggcaacc
     1621 gagcgttctg aacaaatcca gatggagttc tgaggtcatt actggatcta tcaacaggag
     1681 tccaagcgag ctcgatatca aattacgccc cgccctgcca ctcatcgcag tactgttgta
     1741 attcattaag cattctgccg acatggaagc catcacaaac ggcatgatga acctgaatcg
     1801 ccagcggcat cagcaccttg tcgccttgcg tataatattt gcccatggtg aaaacggggg
     1861 cgaagaagtt gtccatattg gccacgttta aatcaaaact ggtgaaactc acccagggat
     1921 tggctgaaac gaaaaacata ttctcaataa accctttagg gaaataggcc aggttttcac
     1981 cgtaacacgc cacatcttgc gaatatatgt gtagaaactg ccggaaatcg tcgtggtatt
     2041 cactccagag cgatgaaaac gtttcagttt gctcatggaa aacggtgtaa caagggtgaa
     2101 cactatccca tatcaccagc tcaccgtctt tcattgccat acgaaattcc ggatgagcat
     2161 tcatcaggcg ggcaagaatg tgaataaagg ccggataaaa cttgtgctta tttttcttta
     2221 cggtctttaa aaaggccgta atatccagct gaacggtctg gttataggta cattgagcaa
     2281 ctgactgaaa tgcctcaaaa tgttctttac gatgccattg ggatatatca acggtggtat
     2341 atccagtgat ttttttctcc attttagctt ccttagctcc tgaaaatctc gataactcaa
     2401 aaaatacgcc cggtagtgat cttatttcat tatggtgaaa gttggaacct cttacgtgcc
     2461 cgatcaatca tgaccaaaat cccttaac
//