LOCUS       MTK4a_017               2491 bp ds-DNA     circular     22-FEB-2019
DEFINITION  .
FEATURES             Location/Qualifiers
     primer_bind     1351..1370
                     /label="P0001_EBFP2-C"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     promoter        complement(2366..2470)
                     /label="CamR Promoter"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     primer_bind     complement(903..924)
                     /label="EGFP-N (Elim)"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     protein_bind    complement(1589..1594)
                     /label="BsaI"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     rep_origin      complement(2471..2491)
                     /label="ColE1"
                     /ApEinfo_revcolor=#7f7f7f
                     /ApEinfo_fwdcolor=#7f7f7f
     misc_feature    768..824
                     /label="P2A"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     protein_bind    748..753
                     /label="BsaI"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    complement(1597..1705)
                     /label="CamR Terminator"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     CDS             complement(1706..2365)
                     /label="CamR"
                     /ApEinfo_revcolor=#0000ff
                     /ApEinfo_fwdcolor=#0000ff
     CDS             861..1574
                     /label="EBFP2"
                     /ApEinfo_revcolor=#0816ff
                     /ApEinfo_fwdcolor=#0816ff
     rep_origin      complement(1..743)
                     /label="ColE1"
                     /ApEinfo_revcolor=#7f7f7f
                     /ApEinfo_fwdcolor=#7f7f7f
     sig_peptide     825..854
                     /label="NLS_(zhang)"
                     /ApEinfo_revcolor=#ff0f00
                     /ApEinfo_fwdcolor=#ff0f00
ORIGIN
        1 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
       61 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
      121 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
      181 gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga
      241 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
      301 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
      361 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
      421 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
      481 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
      541 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
      601 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
      661 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
      721 cccctgattc tgtggataac cgtagtcggt ctcaATCCGG AAGCGGAGCT ACTAACTTCA
      781 GCCTGCTGAA GCAGGCTGGt GACGTGGAGG AGAACCCTGG ACCTCCCAAG AAGAAGAGGA
      841 AGGTGGAGGA CGCCGGATCC GTGAGCAAGG GCGAGGAGCT GTTCACCGGG GTGGTGCCCA
      901 TCCTGGTCGA GCTGGACGGC GACGTAAACG GCCACAAGTT CAGCGTGAGG GGCGAGGGCG
      961 AGGGCGATGC CACCAACGGC AAGCTGACCC TGAAGTTCAT CTGCACCACC GGCAAGCTGC
     1021 CCGTGCCCTG GCCCACCCTC GTGACCACCC TGAGCCACGG CGTGCAGTGC TTCGCCCGCT
     1081 ACCCCGACCA CATGAAGCAG CACGACTTCT TCAAGTCCGC CATGCCCGAA GGCTACGTCC
     1141 AGGAGCGCAC CATCTTCTTC AAGGACGACG GCACCTACAA GACCCGCGCC GAGGTGAAGT
     1201 TCGAGGGCGA CACCCTGGTG AACCGCATCG AGCTGAAGGG CGTCGACTTC AAGGAGGACG
     1261 GCAACATCCT GGGGCACAAG CTGGAGTACA ACTTCAACAG CCACAACATC TATATCATGG
     1321 CCGTCAAGCA GAAGAACGGC ATCAAGGTGA ACTTCAAGAT CCGCCACAAC GTGGAGGACG
     1381 GCAGCGTGCA GCTCGCCGAC CACTACCAGC AGAACACCCC CATCGGCGAC GGCCCCGTGC
     1441 TGCTGCCCGA CAGCCACTAC CTGAGCACCC AGTCCGTGCT GAGCAAAGAC CCCAACGAGA
     1501 AGCGCGATCA CATGGTCCTG CTGGAGTTCC GCACCGCCGC CGGGATCACT CTCGGCATGG
     1561 ACGAGCTGTA CAAGTAACTC GAGTGGCtga gaccagacca ataaaaaacg cccggcggca
     1621 accgagcgtt ctgaacaaat ccagatggag ttctgaggtc attactggat ctatcaacag
     1681 gagtccaagc gagctcgata tcaaattacg ccccgccctg ccactcatcg cagtactgtt
     1741 gtaattcatt aagcattctg ccgacatgga agccatcaca aacggcatga tgaacctgaa
     1801 tcgccagcgg catcagcacc ttgtcgcctt gcgtataata tttgcccatg gtgaaaacgg
     1861 gggcgaagaa gttgtccata ttggccacgt ttaaatcaaa actggtgaaa ctcacccagg
     1921 gattggctga aacgaaaaac atattctcaa taaacccttt agggaaatag gccaggtttt
     1981 caccgtaaca cgccacatct tgcgaatata tgtgtagaaa ctgccggaaa tcgtcgtggt
     2041 attcactcca gagcgatgaa aacgtttcag tttgctcatg gaaaacggtg taacaagggt
     2101 gaacactatc ccatatcacc agctcaccgt ctttcattgc catacgaaat tccggatgag
     2161 cattcatcag gcgggcaaga atgtgaataa aggccggata aaacttgtgc ttatttttct
     2221 ttacggtctt taaaaaggcc gtaatatcca gctgaacggt ctggttatag gtacattgag
     2281 caactgactg aaatgcctca aaatgttctt tacgatgcca ttgggatata tcaacggtgg
     2341 tatatccagt gatttttttc tccattttag cttccttagc tcctgaaaat ctcgataact
     2401 caaaaaatac gcccggtagt gatcttattt cattatggtg aaagttggaa cctcttacgt
     2461 gcccgatcaa tcatgaccaa aatcccttaa c
//