LOCUS       MTK4a_021               2611 bp ds-DNA     circular     22-FEB-2019
DEFINITION  .
ACCESSION   <unknown id>                                                       
VERSION     <unknown id>                                                       
FEATURES             Location/Qualifiers
     rep_origin      complement(join(2591..2611,1..743))
                     /label="ColE1"
                     /ApEinfo_revcolor=#7f7f7f
                     /ApEinfo_fwdcolor=#7f7f7f
     misc_feature    1578..1694
                     /label="d4PEST (MODC)"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     protein_bind    748..753
                     /label="BsaI"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     CDS             complement(1826..2485)
                     /label="CamR"
                     /ApEinfo_revcolor=#0000ff
                     /ApEinfo_fwdcolor=#0000ff
     CDS             864..1571
                     /label="mRuby2"
                     /ApEinfo_revcolor=#ff454e
                     /ApEinfo_fwdcolor=#ff454e
     protein_bind    complement(1709..1714)
                     /label="BsaI"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     promoter        complement(2486..2590)
                     /label="CamR Promoter"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_signal     759..821
                     /label="T2A"
                     /ApEinfo_revcolor=#0080ff
                     /ApEinfo_fwdcolor=#0080ff
     misc_feature    complement(1717..1825)
                     /label="CamR Terminator"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     sig_peptide     828..857
                     /label="NLS_(zhang)"
                     /ApEinfo_revcolor=#ff0f00
                     /ApEinfo_fwdcolor=#ff0f00
ORIGIN
        1 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
       61 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
      121 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
      181 gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga
      241 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
      301 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
      361 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
      421 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
      481 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
      541 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
      601 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
      661 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
      721 cccctgattc tgtggataac cgtagtcggt ctcaATCCGG AAGCGGAGAG GGCAGAGGAA
      781 GTCTGCTAAC ATGCGGTGAC GTCGAGGAGA ATCCTGGACC TGGATCCCCC AAGAAGAAGA
      841 GGAAGGTGGA GGACGCCGGA TCCgtgtcca aaggagagga gttaatcaag gaaaacatga
      901 gaatgaaagt tgtcatggag ggctccgtta atggtcacca attcaagtgt acaggggaag
      961 gtgaaggtaa tccttacatg ggtacacaaa ctatgagaat taaagtaatt gaaggcggac
     1021 cactaccatt tgcatttgac attctggcaa cgtcattcat gtacggatca cgaactttca
     1081 tcaagtaccc taaaggtata ccagactttt tcaagcaatc ttttccagag ggttttacat
     1141 gggaaagggt tacaagatac gaagatgggg gtgtcgtcac agttatgcaa gatacttcat
     1201 tagaagatgg ctgccttgtc tatcatgtgc aagtaagagg ggtgaatttt ccttctaacg
     1261 gacctgtgat gcagaaaaag accaaaggtt gggaaccaaa tactgaaatg atgtacccag
     1321 ctgatggagg tttgagaggc tacacacaca tggcgcttaa agttgatggt ggaggtcatt
     1381 tgtcttgtag ttttgttacc acttatcgtt ctaaaaagac tgttggcaat atcaaaatgc
     1441 caggaataca tgctgtagac cacagactag aaagactcga agagagcgat aacgaaatgt
     1501 tcgttgtaca gagagagcat gccgtagcca aatttgctgg cttaggcggt ggtatggatg
     1561 aattgtataa gggaTCCCAT GGCTTCCCGC CGGAGGTGGA GGAGCAGGAT GATGGCGCGC
     1621 TGCCCATGTC TTGTGCCCAG GAGAGCGGGA TGGACCGTCA CCCTGCAGCC TGTGCTTCTG
     1681 CTAGGATCAA TGTGTAACTC GAGTGGCtga gaccagacca ataaaaaacg cccggcggca
     1741 accgagcgtt ctgaacaaat ccagatggag ttctgaggtc attactggat ctatcaacag
     1801 gagtccaagc gagctcgata tcaaattacg ccccgccctg ccactcatcg cagtactgtt
     1861 gtaattcatt aagcattctg ccgacatgga agccatcaca aacggcatga tgaacctgaa
     1921 tcgccagcgg catcagcacc ttgtcgcctt gcgtataata tttgcccatg gtgaaaacgg
     1981 gggcgaagaa gttgtccata ttggccacgt ttaaatcaaa actggtgaaa ctcacccagg
     2041 gattggctga aacgaaaaac atattctcaa taaacccttt agggaaatag gccaggtttt
     2101 caccgtaaca cgccacatct tgcgaatata tgtgtagaaa ctgccggaaa tcgtcgtggt
     2161 attcactcca gagcgatgaa aacgtttcag tttgctcatg gaaaacggtg taacaagggt
     2221 gaacactatc ccatatcacc agctcaccgt ctttcattgc catacgaaat tccggatgag
     2281 cattcatcag gcgggcaaga atgtgaataa aggccggata aaacttgtgc ttatttttct
     2341 ttacggtctt taaaaaggcc gtaatatcca gctgaacggt ctggttatag gtacattgag
     2401 caactgactg aaatgcctca aaatgttctt tacgatgcca ttgggatata tcaacggtgg
     2461 tatatccagt gatttttttc tccattttag cttccttagc tcctgaaaat ctcgataact
     2521 caaaaaatac gcccggtagt gatcttattt cattatggtg aaagttggaa cctcttacgt
     2581 gcccgatcaa tcatgaccaa aatcccttaa c
//