LOCUS       MTK4b_002               1796 bp ds-DNA     circular     22-FEB-2019
DEFINITION  .
ACCESSION   <unknown id>                                                       
VERSION     <unknown id>                                                       
KEYWORDS    "Name:SV40 Polyadenylation signal" "Part:Part4b - Terminator"      
            "Location:1" "Resistance:Cam"
FEATURES             Location/Qualifiers
     CDS             complement(266..925)
                     /label="CamR"
                     /ApEinfo_revcolor=#0000ff
                     /ApEinfo_fwdcolor=#0000ff
     protein_bind    complement(149..154)
                     /label="BsaI"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     Terminator      14..143
                     /label="SV40-PA"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     protein_bind    3..8
                     /label="BsaI"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    complement(157..265)
                     /label="CamR Terminator"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     promoter        complement(926..1030)
                     /label="CamR Promoter"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     rep_origin      complement(1031..1794)
                     /label="ColE1"
                     /ApEinfo_revcolor=#7f7f7f
                     /ApEinfo_fwdcolor=#7f7f7f
ORIGIN
        1 tcggtctcaT GGCgatccag acatgataag atacattgat gagtttggac aaaccacaac
       61 tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt gatgctattg ctttatttgt
      121 aaccattata agctgcaata aacGCTGtga gaccagacca ataaaaaacg cccggcggca
      181 accgagcgtt ctgaacaaat ccagatggag ttctgaggtc attactggat ctatcaacag
      241 gagtccaagc gagctcgata tcaaattacg ccccgccctg ccactcatcg cagtactgtt
      301 gtaattcatt aagcattctg ccgacatgga agccatcaca aacggcatga tgaacctgaa
      361 tcgccagcgg catcagcacc ttgtcgcctt gcgtataata tttgcccatg gtgaaaacgg
      421 gggcgaagaa gttgtccata ttggccacgt ttaaatcaaa actggtgaaa ctcacccagg
      481 gattggctga aacgaaaaac atattctcaa taaacccttt agggaaatag gccaggtttt
      541 caccgtaaca cgccacatct tgcgaatata tgtgtagaaa ctgccggaaa tcgtcgtggt
      601 attcactcca gagcgatgaa aacgtttcag tttgctcatg gaaaacggtg taacaagggt
      661 gaacactatc ccatatcacc agctcaccgt ctttcattgc catacgaaat tccggatgag
      721 cattcatcag gcgggcaaga atgtgaataa aggccggata aaacttgtgc ttatttttct
      781 ttacggtctt taaaaaggcc gtaatatcca gctgaacggt ctggttatag gtacattgag
      841 caactgactg aaatgcctca aaatgttctt tacgatgcca ttgggatata tcaacggtgg
      901 tatatccagt gatttttttc tccattttag cttccttagc tcctgaaaat ctcgataact
      961 caaaaaatac gcccggtagt gatcttattt cattatggtg aaagttggaa cctcttacgt
     1021 gcccgatcaa tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
     1081 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
     1141 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     1201 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta
     1261 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     1321 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     1381 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     1441 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
     1501 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     1561 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     1621 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
     1681 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
     1741 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtag
//