LOCUS       MTK4b_004               1703 bp ds-DNA     circular     22-FEB-2019
DEFINITION  .
ACCESSION   <unknown id>                                                       
VERSION     <unknown id>                                                       
FEATURES             Location/Qualifiers
     misc_feature    complement(467..575)
                     /label="CamR Terminator"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     protein_bind    complement(459..464)
                     /label="BsaI"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     protein_bind    406..411
                     /label="BsaI"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     promoter        complement(1236..1340)
                     /label="CamR Promoter"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     rep_origin      complement(join(1341..1703,1..401))
                     /label="ColE1"
                     /ApEinfo_revcolor=#7f7f7f
                     /ApEinfo_fwdcolor=#7f7f7f
     CDS             complement(576..1235)
                     /label="CamR"
                     /ApEinfo_revcolor=#0000ff
                     /ApEinfo_fwdcolor=#0000ff
     misc_feature    417..453
                     /label="Spacer"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
ORIGIN
        1 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
       61 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
      121 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
      181 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
      241 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
      301 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
      361 tgttctttcc tgcgttatcc cctgattctg tggataaccg tagtcggtct caTGGCacta
      421 gagatctatg tgaggatcct aactcgagat cgaGCTGtga gaccagacca ataaaaaacg
      481 cccggcggca accgagcgtt ctgaacaaat ccagatggag ttctgaggtc attactggat
      541 ctatcaacag gagtccaagc gagctcgata tcaaattacg ccccgccctg ccactcatcg
      601 cagtactgtt gtaattcatt aagcattctg ccgacatgga agccatcaca aacggcatga
      661 tgaacctgaa tcgccagcgg catcagcacc ttgtcgcctt gcgtataata tttgcccatg
      721 gtgaaaacgg gggcgaagaa gttgtccata ttggccacgt ttaaatcaaa actggtgaaa
      781 ctcacccagg gattggctga aacgaaaaac atattctcaa taaacccttt agggaaatag
      841 gccaggtttt caccgtaaca cgccacatct tgcgaatata tgtgtagaaa ctgccggaaa
      901 tcgtcgtggt attcactcca gagcgatgaa aacgtttcag tttgctcatg gaaaacggtg
      961 taacaagggt gaacactatc ccatatcacc agctcaccgt ctttcattgc catacgaaat
     1021 tccggatgag cattcatcag gcgggcaaga atgtgaataa aggccggata aaacttgtgc
     1081 ttatttttct ttacggtctt taaaaaggcc gtaatatcca gctgaacggt ctggttatag
     1141 gtacattgag caactgactg aaatgcctca aaatgttctt tacgatgcca ttgggatata
     1201 tcaacggtgg tatatccagt gatttttttc tccattttag cttccttagc tcctgaaaat
     1261 ctcgataact caaaaaatac gcccggtagt gatcttattt cattatggtg aaagttggaa
     1321 cctcttacgt gcccgatcaa tcatgaccaa aatcccttaa cgtgagtttt cgttccactg
     1381 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
     1441 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
     1501 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
     1561 tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
     1621 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
     1681 taccgggttg gactcaagac gat
//