LOCUS       MTK5_008                2448 bp ds-DNA     circular     22-FEB-2019
DEFINITION  .
ACCESSION   <unknown id>                                                       
VERSION     <unknown id>                                                       
KEYWORDS    "ConRE':Connector"                                                 
FEATURES             Location/Qualifiers
     protein_bind    complement(1659..1662)
                     /label="BsaI"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     protein_bind    complement(1664..1669)
                     /label="BsaI"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     protein_bind    1020..1025
                     /label="BsmBI"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     CDS             complement(1781..2440)
                     /label="CamR"
                     /ApEinfo_revcolor=#0000ff
                     /ApEinfo_fwdcolor=#0000ff
     terminator      complement(1672..1780)
                     /label="CamR Terminator"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     promoter        complement(join(2441..2448,1..97))
                     /label="CamR Promoter"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     rep_origin      complement(98..861)
                     /label="ColE1"
                     /ApEinfo_revcolor=#7f7f7f
                     /ApEinfo_fwdcolor=#7f7f7f
     protein_bind    866..871
                     /label="BsaI"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    877..1019
                     /label="ConE'"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    1364..1643
                     /label="HS4"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     protein_bind    873..876
                     /label="BsaI"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    1081..1360
                     /label="HS4"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    1027..1051
                     /label="ConE scar"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     protein_bind    1027..1030
                     /label="BsmBI"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
ORIGIN
        1 ccttagctcc tgaaaatctc gataactcaa aaaatacgcc cggtagtgat cttatttcat
       61 tatggtgaaa gttggaacct cttacgtgcc cgatcaatca tgaccaaaat cccttaacgt
      121 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
      181 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
      241 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
      301 gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac
      361 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
      421 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
      481 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
      541 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
      601 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
      661 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
      721 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
      781 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
      841 cctgattctg tggataaccg tagtcggtct cagctggaaa tctgctcgtc agtggtgctc
      901 acactgacga atcatgtaca gatcataccg atgactgcct ggcgactcac aactaagcaa
      961 gacagccgga accagcgccg gcgaacacca ctgcatatat ggcatatcac aacagtccac
     1021 gtctcaagca gttacagaga tgttacgaac cactagtgca ctgcagATCG AATTCGAGTT
     1081 GGCGCGCCTG TCATTCTAAA TCTCTCTTTC AGCCTAAAGC TTTTTCCCCG TATCCCCCCA
     1141 GGTGTCTGCA GGCTCAAAGA GCAGCGAGAA GCGTTCAGAG GAAAGCGATC CCGTGCCACC
     1201 TTCCCCGTGC CCGGGCTGTC CCCGCACGCT GCCGGCTCGG GGATGCGGGG GGAGCGCCGG
     1261 ACCGGAGCGG AGCCCCGGGC GGCTCGCTGC TGCCCCCTAG CGGGGGAGGG ACGTAATTAC
     1321 ATCCCTGGGG GCTTTGGGAG GGGGCTGTCC CCGTGAGCTC CCAGGCGCGC CTGTCATTCT
     1381 AAATCTCTCT TTCAGCCTAA AGCTTTTTCC CCGTATCCCC CCAGGTGTCT GCAGGCTCAA
     1441 AGAGCAGCGA GAAGCGTTCA GAGGAAAGCG ATCCCGTGCC ACCTTCCCCG TGCCCGGGCT
     1501 GTCCCCGCAC GCTGCCGGCT CGGGGATGCG GGGGGAGCGC CGGACCGGAG CGGAGCCCCG
     1561 GGCGGCTCGC TGCTGCCCCC TAGCGGGGGA GGGACGTAAT TACATCCCTG GGGGCTTTGG
     1621 GGGGGGGCTG TCCCCGTGAG CTCAATAAAA GAGCCCACta catgagacca gaccaataaa
     1681 aaacgcccgg cggcaaccga gcgttctgaa caaatccaga tggagttctg aggtcattac
     1741 tggatctatc aacaggagtc caagcgagct cgatatcaaa ttacgccccg ccctgccact
     1801 catcgcagta ctgttgtaat tcattaagca ttctgccgac atggaagcca tcacaaacgg
     1861 catgatgaac ctgaatcgcc agcggcatca gcaccttgtc gccttgcgta taatatttgc
     1921 ccatggtgaa aacgggggcg aagaagttgt ccatattggc cacgtttaaa tcaaaactgg
     1981 tgaaactcac ccagggattg gctgaaacga aaaacatatt ctcaataaac cctttaggga
     2041 aataggccag gttttcaccg taacacgcca catcttgcga atatatgtgt agaaactgcc
     2101 ggaaatcgtc gtggtattca ctccagagcg atgaaaacgt ttcagtttgc tcatggaaaa
     2161 cggtgtaaca agggtgaaca ctatcccata tcaccagctc accgtctttc attgccatac
     2221 gaaattccgg atgagcattc atcaggcggg caagaatgtg aataaaggcc ggataaaact
     2281 tgtgcttatt tttctttacg gtctttaaaa aggccgtaat atccagctga acggtctggt
     2341 tataggtaca ttgagcaact gactgaaatg cctcaaaatg ttctttacga tgccattggg
     2401 atatatcaac ggtggtatat ccagtgattt ttttctccat tttagctt
//