LOCUS       MTK6_004                3671 bp ds-DNA     circular     22-FEB-2019
DEFINITION  .
FEATURES             Location/Qualifiers
     sig_peptide     2086..2115
                     /label="NLS_(zhang)"
                     /ApEinfo_revcolor=#ff0f00
                     /ApEinfo_fwdcolor=#ff0f00
     Terminator      2602..2837
                     /label="BGH-PA"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     primer_bind     1316..1336
                     /label="CMV-F (Elim)"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     rep_origin      complement(52..815)
                     /label="ColE1"
                     /ApEinfo_revcolor=#7f7f7f
                     /ApEinfo_fwdcolor=#7f7f7f
     CDS             complement(2958..3617)
                     /label="CamR"
                     /ApEinfo_revcolor=#0000ff
                     /ApEinfo_fwdcolor=#0000ff
     misc_feature    1387..2085
                     /label="TagBFP"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     promoter        complement(join(3618..3671,1..51))
                     /label="CamR Promoter"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     CDS             2200..2593
                     /label="Blast"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     protein_bind    2843..2846
                     /label="BsmBI"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_signal     2128..2190
                     /label="T2A"
                     /ApEinfo_revcolor=#0080ff
                     /ApEinfo_fwdcolor=#0080ff
     misc_feature    complement(2849..2957)
                     /label="CamR Terminator"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     promoter        859..1384
                     /label="CMV promoter"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
ORIGIN
        1 tgatcttatt tcattatggt gaaagttgga acctcttacg tgcccgatca atcatgacca
       61 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
      121 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
      181 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
      241 ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc
      301 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
      361 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
      421 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
      481 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
      541 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
      601 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
      661 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
      721 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
      781 ttcctgcgtt atcccctgat tctgtggata accgtagTCG GTCTCATACA GCGGTGGGCT
      841 CTATGGggta cctacgtagg tacataactt acggtaaatg gcccgcctgg ctgaccgccc
      901 aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg
      961 actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat
     1021 caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc
     1081 tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta catctacgta
     1141 ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag
     1201 cggtttgact cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt
     1261 tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa
     1321 atgggcggta ggcgtgtacg gtgggaggtc tatataagca gagctcgttt agtgaaccgt
     1381 cagataATGA GCGAGCTGAT TAAGGAGAAC ATGCACATGA AGCTGTACAT GGAGGGCACC
     1441 GTGGACAACC ATCACTTCAA GTGCACATCC GAGGGCGAAG GCAAGCCCTA CGAGGGCACC
     1501 CAGACCATGA GAATCAAGGT GGTCGAGGGC GGCCCTCTCC CCTTCGCCTT CGACATCCTG
     1561 GCTACTAGCT TCCTCTACGG CAGCAAGACC TTCATCAACC ACACCCAGGG CATCCCCGAC
     1621 TTCTTCAAGC AGTCCTTCCC TGAGGGCTTC ACATGGGAGA GAGTCACCAC ATACGAAGAC
     1681 GGGGGCGTGC TGACCGCTAC CCAGGACACC AGCCTCCAGG ACGGCTGCCT CATCTACAAC
     1741 GTCAAGATCA GAGGGGTGAA CTTCACATCC AACGGCCCTG TGATGCAGAA GAAAACACTC
     1801 GGCTGGGAGG CCTTCACCGA aACGCTGTAC CCCGCTGACG GCGGCCTGGA AGGCAGAAAC
     1861 GACATGGCCC TGAAGCTCGT GGGCGGGAGC CATCTGATCG CAAACATCAA GACCACATAT
     1921 AGATCCAAGA AACCCGCTAA GAACCTCAAG ATGCCTGGCG TCTACTATGT GGACTACAGA
     1981 CTGGAAAGAA TCAAGGAGGC CAACAACGAa ACCTACGTCG AGCAGCACGA GGTGGCAGTG
     2041 GCCAGATACT GCGACCTCCC TAGCAAACTG GGGCACAAGC TTAATCCCAA GAAGAAGAGG
     2101 AAGGTGGAGG ACGCCCGTAC GAGTACTGGA AGCGGAGAGG GCAGAGGAAG TCTGCTAACA
     2161 TGCGGTGACG TCGAGGAGAA TCCTGGACCT GGATCCATGG CCAAGCCTTT GTCTCAAGAA
     2221 GAATCCACCC TCATTGAAAG AGCAACGGCT ACAATCAACA GCATCCCCAT CTCTGAAGAC
     2281 TACAGCGTCG CCAGCGCAGC TCTCTCTAGC GACGGCCGCA TCTTCACTGG TGTCAATGTA
     2341 TATCATTTTA CTGGGGGACC TTGTGCAGAA CTCGTGGTGC TGGGCACTGC TGCTGCTGCG
     2401 GCAGCTGGCA ACCTGACTTG TATCGTCGCG ATCGGAAATG AGAACAGGGG CATCTTGAGC
     2461 CCCTGCGGAC GGTGCCGACA GGTGCTTCTC GATCTGCATC CTGGGATCAA AGCCATAGTG
     2521 AAGGACAGTG ATGGACAGCC GACGGCAGTT GGGATTCGTG AATTGCTGCC CTCTGGTTAT
     2581 GTGTGGGAGG GCTAACTCGA GCGACGCCTC GACTGTGCCT TCTAGTTGCC AGCCATCTGT
     2641 TGTTTGCCCC TCCCCCGTGC CTTCCTTGAC CCTGGAAGGT GCCACTCCCA CTGTCCTTTC
     2701 CTAATAAAAT GAGGAAATTG CATCGCATTG TCTGAGTAGG TGTCATTCTA TTCTGGGGGG
     2761 TGGGGTGGGG CAGGACAGCA AGGGGGAGGA TTGGGAAGAC AATAGCAGGC ATGCTGGGGA
     2821 TGCGGTGGGC TCTATGAGTt gagaccagac caataaaaaa cgcccggcgg caaccgagcg
     2881 ttctgaacaa atccagatgg agttctgagg tcattactgg atctatcaac aggagtccaa
     2941 gcgagctcga tatcaaatta cgccccgccc tgccactcat cgcagtactg ttgtaattca
     3001 ttaagcattc tgccgacatg gaagccatca caaacggcat gatgaacctg aatcgccagc
     3061 ggcatcagca ccttgtcgcc ttgcgtataa tatttgccca tggtgaaaac gggggcgaag
     3121 aagttgtcca tattggccac gtttaaatca aaactggtga aactcaccca gggattggct
     3181 gaaacgaaaa acatattctc aataaaccct ttagggaaat aggccaggtt ttcaccgtaa
     3241 cacgccacat cttgcgaata tatgtgtaga aactgccgga aatcgtcgtg gtattcactc
     3301 cagagcgatg aaaacgtttc agtttgctca tggaaaacgg tgtaacaagg gtgaacacta
     3361 tcccatatca ccagctcacc gtctttcatt gccatacgaa attccggatg agcattcatc
     3421 aggcgggcaa gaatgtgaat aaaggccgga taaaacttgt gcttattttt ctttacggtc
     3481 tttaaaaagg ccgtaatatc cagctgaacg gtctggttat aggtacattg agcaactgac
     3541 tgaaatgcct caaaatgttc tttacgatgc cattgggata tatcaacggt ggtatatcca
     3601 gtgatttttt tctccatttt agcttcctta gctcctgaaa atctcgataa ctcaaaaaat
     3661 acgcccggta g
//