LOCUS       MTK6_009                3497 bp ds-DNA     circular     22-FEB-2019
DEFINITION  .
FEATURES             Location/Qualifiers
     rep_origin      complement(22..785)
                     /label="ColE1"
                     /ApEinfo_revcolor=#7f7f7f
                     /ApEinfo_fwdcolor=#7f7f7f
     promoter        complement(join(3414..3497,1..21))
                     /label="CamR Promoter"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     CDS             complement(2754..3413)
                     /label="CamR"
                     /ApEinfo_revcolor=#0000ff
                     /ApEinfo_fwdcolor=#0000ff
     misc_feature    1357..1365
                     /label="kozac"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     CDS             1366..2388
                     /label="HPH (Hygromycin Phosphotransferase)"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     promoter        829..1354
                     /label="CMV promoter"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    complement(2645..2753)
                     /label="CamR Terminator"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     Terminator      2398..2631
                     /label="BGH-PA"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     protein_bind    2639..2642
                     /label="BsmBI"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
ORIGIN
        1 acctcttacg tgcccgatca atcatgacca aaatccctta acgtgagttt tcgttccact
       61 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
      121 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
      181 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
      241 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
      301 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
      361 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
      421 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
      481 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
      541 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
      601 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
      661 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
      721 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
      781 accgtagTCG GTCTCATACA GCGGTGGGCT CTATGGGGTA CCTACGTAgg tacataactt
      841 acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg
      901 acgtatgttc ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat
      961 ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct
     1021 attgacgtca atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg
     1081 gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg
     1141 ttttggcagt acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc
     1201 caccccattg acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa
     1261 tgtcgtaaca actccgcccc attgaCGCAA ATGGGCGGTA GGCGTGtacg gtgggaggtc
     1321 tatataagca gagctcgttt agtgaaccgt cagataGCCA CCATGggtaa aaagcctgaa
     1381 ctcaccgcga cgtctgtcga gaagtttctg atcgaaaagt tcgacagcgt gtccgacctg
     1441 atgcagctct cggagggcga agaatctcgt gctttcagct tcgatgtagg agggcgtgga
     1501 tatgtcctgc gggtaaatag ctgcgccgat ggtttctaca aagatcgtta tgtttatcgg
     1561 cactttgcat cggccgcgct cccgattccg gaagtgcttg acattgggga atttagcgag
     1621 agcctgacct attgcatctc ccgccgtgca cagggtgtca cgttgcaaga cctgcctgaa
     1681 accgaactgc ccgctgttct gcaaccggtc gcggaggcca tggatgcgat cgctgcggcc
     1741 gatcttagcc agacgagcgg gttcggccca ttcggaccgc aaggaatcgg tcaatacact
     1801 acatggcgtg atttcatatg cgcgattgct gatccccatg tgtatcactg gcaaactgtg
     1861 atggacgaca ccgtcagtgc gtccgtcgcg caggctctcg atgagctgat gctttgggcc
     1921 gaggactgcc ccgaagtccg gcacctcgtg cacgcggatt tcggctccaa caatgtcctg
     1981 acggacaatg gccgcataac agcggtcatt gactggagcg aggcgatgtt cggggattcc
     2041 caatacgagg tcgccaacat cttcttctgg aggccgtggt tggcttgtat ggagcagcag
     2101 acgcgctact tcgagcggag gcatccggag cttgcaggat cgccgcggct ccgggcgtat
     2161 atgctccgca ttggtcttga ccaactctat cagagcttgg ttgacggcaa tttcgatgat
     2221 gcagcttggg cgcagggtcg atgcgacgca atcgtccgat ccggagccgg gactgtcggg
     2281 cgtacacaaa tcgcccgcag aagcgcggcc gtctggaccg atggctgtgt agaagtactc
     2341 gccgatagtg gaaaccgacg ccccagcact cgtccgaggg caaaggaatA ACTCGAGCGA
     2401 CGCCTCGACT GTGCCTTCTA GTTGCCAGCC ATCTGTTGTT TGCCCCTCCC CCGTGCCTTC
     2461 CTTGACCCTG GAAGGTGCCA CTCCCACTGT CCTTTCCTAA TAAAATGAGG AAATTGCATC
     2521 GCATTGTCTG AGTAGGTGTC ATTCTATTCT GGGGGGTGGG GTGGGGCAGG ACAGCAAGGG
     2581 GGAGGATTGG GAAGACAATA GCAGGCATGC TGGGGATGCG GTGGGCTCTA TGAGTtgaga
     2641 ccagaccaat aaaaaacgcc cggcggcaac cgagcgttct gaacaaatcc agatggagtt
     2701 ctgaggtcat tactggatct atcaacagga gtccaagcga gctcgatatc aaattacgcc
     2761 ccgccctgcc actcatcgca gtactgttgt aattcattaa gcattctgcc gacatggaag
     2821 ccatcacaaa cggcatgatg aacctgaatc gccagcggca tcagcacctt gtcgccttgc
     2881 gtataatatt tgcccatggt gaaaacgggg gcgaagaagt tgtccatatt ggccacgttt
     2941 aaatcaaaac tggtgaaact cacccaggga ttggctgaaa cgaaaaacat attctcaata
     3001 aaccctttag ggaaataggc caggttttca ccgtaacacg ccacatcttg cgaatatatg
     3061 tgtagaaact gccggaaatc gtcgtggtat tcactccaga gcgatgaaaa cgtttcagtt
     3121 tgctcatgga aaacggtgta acaagggtga acactatccc atatcaccag ctcaccgtct
     3181 ttcattgcca tacgaaattc cggatgagca ttcatcaggc gggcaagaat gtgaataaag
     3241 gccggataaa acttgtgctt atttttcttt acggtcttta aaaaggccgt aatatccagc
     3301 tgaacggtct ggttataggt acattgagca actgactgaa atgcctcaaa atgttcttta
     3361 cgatgccatt gggatatatc aacggtggta tatccagtga tttttttctc cattttagct
     3421 tccttagctc ctgaaaatct cgataactca aaaaatacgc ccggtagtga tcttatttca
     3481 ttatggtgaa agttgga
//