LOCUS       MTK7_010                3115 bp ds-DNA     circular     22-FEB-2019
DEFINITION  .
ACCESSION   <unknown id>                                                       
VERSION     <unknown id>                                                       
FEATURES             Location/Qualifiers
     promoter        complement(2945..3049)
                     /label="CamR Promoter"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    complement(2176..2284)
                     /label="CamR Terminator"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    2170..2173
                     /label="BsmBI"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    714..1412
                     /label="TagBFP"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     sig_peptide     1413..1442
                     /label="NLS_(zhang)"
                     /ApEinfo_revcolor=#ff0f00
                     /ApEinfo_fwdcolor=#ff0f00
     rep_origin      complement(join(3050..3115,1..698))
                     /label="ColE1"
                     /ApEinfo_revcolor=#7f7f7f
                     /ApEinfo_fwdcolor=#7f7f7f
     CDS             1527..1920
                     /label="Blast"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_signal     1455..1517
                     /label="T2A"
                     /ApEinfo_revcolor=#0080ff
                     /ApEinfo_fwdcolor=#0080ff
     protein_bind    complement(701..704)
                     /label="BsmBI"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     CDS             complement(2285..2944)
                     /label="CamR"
                     /ApEinfo_revcolor=#0000ff
                     /ApEinfo_fwdcolor=#0000ff
ORIGIN
        1 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
       61 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
      121 taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag
      181 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
      241 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
      301 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
      361 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
      421 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
      481 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
      541 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
      601 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
      661 tctttcctgc gttatcccct gattctgtgg ataaccgtag tcggTCTCAG AGTATGAGCG
      721 AGCTGATTAA GGAGAACATG CACATGAAGC TGTACATGGA GGGCACCGTG GACAACCATC
      781 ACTTCAAGTG CACATCCGAG GGCGAAGGCA AGCCCTACGA GGGCACCCAG ACCATGAGAA
      841 TCAAGGTGGT CGAGGGCGGC CCTCTCCCCT TCGCCTTCGA CATCCTGGCT ACTAGCTTCC
      901 TCTACGGCAG CAAGACCTTC ATCAACCACA CCCAGGGCAT CCCCGACTTC TTCAAGCAGT
      961 CCTTCCCTGA GGGCTTCACA TGGGAGAGAG TCACCACATA CGAAGACGGG GGCGTGCTGA
     1021 CCGCTACCCA GGACACCAGC CTCCAGGACG GCTGCCTCAT CTACAACGTC AAGATCAGAG
     1081 GGGTGAACTT CACATCCAAC GGCCCTGTGA TGCAGAAGAA AACACTCGGC TGGGAGGCCT
     1141 TCACCGAaAC GCTGTACCCC GCTGACGGCG GCCTGGAAGG CAGAAACGAC ATGGCCCTGA
     1201 AGCTCGTGGG CGGGAGCCAT CTGATCGCAA ACATCAAGAC CACATATAGA TCCAAGAAAC
     1261 CCGCTAAGAA CCTCAAGATG CCTGGCGTCT ACTATGTGGA CTACAGACTG GAAAGAATCA
     1321 AGGAGGCCAA CAACGAaACC TACGTCGAGC AGCACGAGGT GGCAGTGGCC AGATACTGCG
     1381 ACCTCCCTAG CAAACTGGGG CACAAGCTTA ATCCCAAGAA GAAGAGGAAG GTGGAGGACG
     1441 CCCGTACGAG TACTGGAAGC GGAGAGGGCA GAGGAAGTCT GCTAACATGC GGTGACGTCG
     1501 AGGAGAATCC TGGACCTGGA TCCATGGCCA AGCCTTTGTC TCAAGAAGAA TCCACCCTCA
     1561 TTGAAAGAGC AACGGCTACA ATCAACAGCA TCCCCATCTC TGAAGACTAC AGCGTCGCCA
     1621 GCGCAGCTCT CTCTAGCGAC GGCCGCATCT TCACTGGTGT CAATGTATAT CATTTTACTG
     1681 GGGGACCTTG TGCAGAACTC GTGGTGCTGG GCACTGCTGC TGCTGCGGCA GCTGGCAACC
     1741 TGACTTGTAT CGTCGCGATC GGAAATGAGA ACAGGGGCAT CTTGAGCCCC TGCGGACGGT
     1801 GCCGACAGGT GCTTCTCGAT CTGCATCCTG GGATCAAAGC CATAGTGAAG GACAGTGATG
     1861 GACAGCCGAC GGCAGTTGGG ATTCGTGAAT TGCTGCCCTC TGGTTATGTG TGGGAGGGCT
     1921 AACTCGAGCG ACGCCTCGAC TGTGCCTTCT AGTTGCCAGC CATCTGTTGT TTGCCCCTCC
     1981 CCCGTGCCTT CCTTGACCCT GGAAGGTGCC ACTCCCACTG TCCTTTCCTA ATAAAATGAG
     2041 GAAATTGCAT CGCATTGTCT GAGTAGGTGT CATTCTATTC TGGGGGGTGG GGTGGGGCAG
     2101 GACAGCAAGG GGGAGGATTG GGAAGACAAT AGCAGGCATG CTGGGGATGC GGTGGGCTCT
     2161 ATccgatgag accagaccaa taaaaaacgc ccggcggcaa ccgagcgttc tgaacaaatc
     2221 cagatggagt tctgaggtca ttactggatc tatcaacagg agtccaagcg agctcgatat
     2281 caaattacgc cccgccctgc cactcatcgc agtactgttg taattcatta agcattctgc
     2341 cgacatggaa gccatcacaa acggcatgat gaacctgaat cgccagcggc atcagcacct
     2401 tgtcgccttg cgtataatat ttgcccatgg tgaaaacggg ggcgaagaag ttgtccatat
     2461 tggccacgtt taaatcaaaa ctggtgaaac tcacccaggg attggctgaa acgaaaaaca
     2521 tattctcaat aaacccttta gggaaatagg ccaggttttc accgtaacac gccacatctt
     2581 gcgaatatat gtgtagaaac tgccggaaat cgtcgtggta ttcactccag agcgatgaaa
     2641 acgtttcagt ttgctcatgg aaaacggtgt aacaagggtg aacactatcc catatcacca
     2701 gctcaccgtc tttcattgcc atacgaaatt ccggatgagc attcatcagg cgggcaagaa
     2761 tgtgaataaa ggccggataa aacttgtgct tatttttctt tacggtcttt aaaaaggccg
     2821 taatatccag ctgaacggtc tggttatagg tacattgagc aactgactga aatgcctcaa
     2881 aatgttcttt acgatgccat tgggatatat caacggtggt atatccagtg atttttttct
     2941 ccattttagc ttccttagct cctgaaaatc tcgataactc aaaaaatacg cccggtagtg
     3001 atcttatttc attatggtga aagttggaac ctcttacgtg cccgatcaat catgaccaaa
     3061 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatca
//