LOCUS       MTK8a_005               3145 bp ds-DNA     circular     22-FEB-2019
DEFINITION  .
ACCESSION   <unknown id>                                                       
VERSION     <unknown id>                                                       
KEYWORDS    "KanR-ColE1:ECMarkerOri-8a"                                        
FEATURES             Location/Qualifiers
     CDS             1485..2162
                     /label="mRFP1"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     protein_bind    2312..2315
                     /label="BsaI"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    2329..2769
                     /label="F1 ori"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     terminator      2172..2300
                     /label="BBa_B0015 Terminator"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     protein_bind    2305..2310
                     /label="BsaI"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     rep_origin      2341..2647
                     /label="F1 ori"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     rep_origin      complement(621..1384)
                     /label="ColE1"
                     /ApEinfo_revcolor=#7f7f7f
                     /ApEinfo_fwdcolor=#7f7f7f
     terminator      complement(2775..2801)
                     /label="KanR Terminator"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     RBS             1460..1484
                     /label="mRFP1 Ribsosome Binding Site"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     protein_bind    complement(1393..1396)
                     /label="BsaI"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     CDS             complement(join(2802..3145,1..472))
                     /label="KanR"
                     /ApEinfo_revcolor=#008000
                     /ApEinfo_fwdcolor=#008000
     promoter        1406..1459
                     /label="Bba_R0040 TetR Promoter"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     promoter        complement(473..620)
                     /label="KanR Promoter"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     protein_bind    complement(1398..1403)
                     /label="BsaI"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
ORIGIN
        1 tattcattcg tgattgcgcc tgagcgaggc gaaatacgcg atcgctgtta aaaggacaat
       61 tacaaacagg aatcgaatgc aaccggcgca ggaacactgc cagcgcatca acaatatttt
      121 cacctgaatc aggatattct tctaatacct ggaatgctgt tttcccgggg atcgcagtgg
      181 tgagtaacca tgcatcatca ggagtacgga taaaatgctt gatggtcgga agaggcataa
      241 attccgtcag ccagtttagt ctgaccatct catctgtaac atcattggca acgctacctt
      301 tgccatgttt cagaaacaac tctggcgcat cgggcttccc atacaatcga tagattgtcg
      361 cacctgattg cccgacatta tcgcgagccc atttataccc atataaatca gcatccatgt
      421 tggaatttaa tcgcggcctg gagcaagacg tttcccgttg aatatggctc ataacacccc
      481 ttgtattact gtttatgtaa gcagacagtt ttattgttca tgatgatata tttttatctt
      541 gtgcaatgta acatcagaga ttttgagaca caacgtggct ttgttgaata aatcgaactt
      601 ttgctgagtt gaaggatcag tcatgaccaa aatcccttaa cgtgagtttt cgttccactg
      661 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
      721 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
      781 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
      841 tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
      901 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
      961 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
     1021 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
     1081 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
     1141 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
     1201 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
     1261 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
     1321 cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa
     1381 ccgtgcggcc gccaattgag accagtccct atcagtgata gagattgaca tccctatcag
     1441 tgatagagat actgagcacg gatctgaaag aggagaaagg atctatggcg agtagcgaag
     1501 acgttatcaa agagttcatg cgtttcaaag ttcgtatgga aggttccgtt aacggtcacg
     1561 agttcgaaat cgaaggtgaa ggtgaaggtc gtccgtacga aggtactcag accgctaaac
     1621 tgaaagttac caaaggtggt ccgctgccgt tcgcttggga catcctgtcc ccgcagttcc
     1681 agtacggttc caaagcttac gttaaacacc cggctgacat cccggactac ctgaaactgt
     1741 ccttcccgga aggtttcaaa tgggaacgtg ttatgaactt cgaagacggt ggtgttgtta
     1801 ccgttaccca ggactcctcc ctgcaagacg gtgagttcat ctacaaagtt aaactgcgtg
     1861 gtactaactt cccgtccgac ggtccggtta tgcagaaaaa aaccatgggt tgggaagctt
     1921 ccaccgaacg tatgtacccg gaagacggtg ctctgaaagg tgaaatcaaa atgcgtctga
     1981 aactgaaaga cggtggtcac tacgacgctg aagttaaaac cacctacatg gctaaaaaac
     2041 cggttcagct gccgggtgct tacaaaaccg acatcaaact ggacatcacc tcccacaacg
     2101 aagactacac catcgttgaa cagtacgaac gtgctgaagg tcgtcactcc accggtgctt
     2161 aataaggatc tccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg
     2221 ttttatctgt tgtttgtcgg tgaacgctct ctactagagt cacactggct caccttcggg
     2281 tgggcctttc tgcgtttata agtcggtctc accgagcggc cgcACGCGcc ctgtagcggc
     2341 gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc
     2401 ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc
     2461 cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc
     2521 gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg
     2581 gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact
     2641 ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt
     2701 tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa
     2761 atattaacgc TTACgtgtta caaccaatta accaattctg attagaaaaa ctcatcgagc
     2821 atcaaatgaa actgcaattt attcatatca ggattatcaa taccatattt ttgaaaaagc
     2881 cgtttctgta atgaaggaga aaactcaccg aggcagttcc ataggatggc aagatcctgg
     2941 tatcggtctg cgattccgac tcgtccaaca tcaatacaac ctattaattt cccctcgtca
     3001 aaaataaggt tatcaagtga gaaatcacca tgagtgacga ctgaatccgg tgagaatggc
     3061 aaaagcttat gcatttcttt ccagacttgt tcaacaggcc agccattacg ctcgtcatca
     3121 aaatcactcg catcaaccaa accgt
//