LOCUS       MTK8b_004               3646 bp ds-DNA     circular     22-FEB-2019
DEFINITION  .
ACCESSION   <unknown id>                                                       
VERSION     <unknown id>                                                       
FEATURES             Location/Qualifiers
     CDS             complement(2882..3541)
                     /label="CamR"
                     /ApEinfo_revcolor=#0000ff
                     /ApEinfo_fwdcolor=#0000ff
     promoter        1034..1214
                     /label="trunc_HIV-1_3LTR"
                     /ApEinfo_revcolor=#0080ff
                     /ApEinfo_fwdcolor=#0080ff
     misc_feature    769..775
                     /label="BsaI"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     misc_feature    complement(2773..2881)
                     /label="CamR Terminator"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     rep_origin      complement(1..764)
                     /label="ColE1"
                     /ApEinfo_revcolor=#7f7f7f
                     /ApEinfo_fwdcolor=#7f7f7f
     misc_signal     2612..2627
                     /label="cPPT"
                     /ApEinfo_revcolor=#ff0000
                     /ApEinfo_fwdcolor=#ff0000
     misc_feature    2765..2770
                     /label="BsaI"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     promoter        complement(3542..3646)
                     /label="CamR Promoter"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_signal     1325..1369
                     /label="HIV-1_psi_pack"
                     /ApEinfo_revcolor=#ff0000
                     /ApEinfo_fwdcolor=#ff0000
     promoter        1034..1214
                     /label="HIV-1_5LTR"
                     /ApEinfo_revcolor=#0080ff
                     /ApEinfo_fwdcolor=#0080ff
     CDS             1879..2112
                     /label="RRE_(HIV)"
                     /ApEinfo_revcolor=#0000a0
                     /ApEinfo_fwdcolor=#0000a0
ORIGIN
        1 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
       61 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
      121 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
      181 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
      241 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
      301 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
      361 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
      421 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
      481 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
      541 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
      601 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
      661 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
      721 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtagtcGG TCTCaCAATa
      781 acaaaagctg gagctgcaag cttaatgtag tcttatgcaa tactcttgta gtcttgcaac
      841 atggtaacga tgagttagca acatgcctta caaggagaga aaaagcaccg tgcatgccga
      901 ttggtggaag taaggtggta cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca
      961 tggattggac gaaccactga attgccgcat tgcagagata ttgtatttaa gtgcctagct
     1021 cgatacaata aacggAtctc tctggttaga ccagatctga gcctgggagc tctctggcta
     1081 actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg
     1141 tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg
     1201 gaaaatctct agcagtggcg cccgaacagg gacttgaaag cgaaagggaa accagaggag
     1261 ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga
     1321 ctggtgagta cgccaaaaat tttgactagc ggaggctaga aggagagaga tgggtgcgag
     1381 agcgtcagta ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg gttaaggcca
     1441 gggggaaaga aaaaatataa attaaaacat atagtatggg caagcaggga gctagaacga
     1501 ttcgcagtta atcctggcct gttagaaaca tcagaaggct gtagacaaat actgggacag
     1561 ctacaaccat cccttcagac aggatcagaa gaacttagat cattatataa tacagtagca
     1621 accctctatt gtgtgcatca aaggatagag ataaaagaca ccaaggaagc tttagacaag
     1681 atagaggaag agcaaaacaa aagtaagacc accgcacagc aagcggccgc tgatcttcag
     1741 acctggagga ggagatatga gggacaattg gagaagtgaa ttatataaat ataaagtagt
     1801 aaaaattgaa ccattaggag tagcacccac caaggcaaag agaagagtgg tgcagagaga
     1861 aaaaagagca gtgggaatag gagctttgtt ccttgggttc ttgggagcag caggaagcac
     1921 tatgggcgca gcctcaatga cgctgacggt acaggccaga caattattgt ctggtatagt
     1981 gcagcagcag aacaatttgc tgagggctat tgaggcgcaa cagcatctgt tgcaactcac
     2041 agtctggggc atcaagcagc tccaggcaag aatcctggct gtggaaagat acctaaagga
     2101 tcaacagctc ctggggattt ggggttgctc tggaaaactc atttgcacca ctgctgtgcc
     2161 ttggaatgct agttggagta ataaatctct ggaacagatt tggaatcaca cgacctggat
     2221 ggagtgggac agagaaatta acaattacac aagcttaata cactccttaa ttgaagaatc
     2281 gcaaaaccag caagaaaaga atgaacaaga attattggaa ttagataaat gggcaagttt
     2341 gtggaattgg tttaacataa caaattggct gtggtatata aaattattca taatgatagt
     2401 aggaggcttg gtaggtttaa gaatagtttt tgctgtactt tctatagtga atagagttag
     2461 gcagggatat tcaccattat cgtttcagac ccacctccca accccgaggg gacccgacag
     2521 gcccgaagga atagaagaag aaggtggaga gagagacaga gacagatcca ttcgattagt
     2581 gaacggatct cgacggtatc ggttaacttt taaaagaaaa ggggggattg gggggtacag
     2641 tgcaggggaa agaatagtag acataatagc aacagacata caaactaaag aattacaaaa
     2701 acaaattaca aaaattcaaa attttatcga tcacgagact agcctcgaga agcttgataC
     2761 CCTtGAGACC agaccaataa aaaacgcccg gcggcaaccg agcgttctga acaaatccag
     2821 atggagttct gaggtcatta ctggatctat caacaggagt ccaagcgagc tcgatatcaa
     2881 attacgcccc gccctgccac tcatcgcagt actgttgtaa ttcattaagc attctgccga
     2941 catggaagcc atcacaaacg gcatgatgaa cctgaatcgc cagcggcatc agcaccttgt
     3001 cgccttgcgt ataatatttg cccatggtga aaacgggggc gaagaagttg tccatattgg
     3061 ccacgtttaa atcaaaactg gtgaaactca cccagggatt ggctgaaacg aaaaacatat
     3121 tctcaataaa ccctttaggg aaataggcca ggttttcacc gtaacacgcc acatcttgcg
     3181 aatatatgtg tagaaactgc cggaaatcgt cgtggtattc actccagagc gatgaaaacg
     3241 tttcagtttg ctcatggaaa acggtgtaac aagggtgaac actatcccat atcaccagct
     3301 caccgtcttt cattgccata cgaaattccg gatgagcatt catcaggcgg gcaagaatgt
     3361 gaataaaggc cggataaaac ttgtgcttat ttttctttac ggtctttaaa aaggccgtaa
     3421 tatccagctg aacggtctgg ttataggtac attgagcaac tgactgaaat gcctcaaaat
     3481 gttctttacg atgccattgg gatatatcaa cggtggtata tccagtgatt tttttctcca
     3541 ttttagcttc cttagctcct gaaaatctcg ataactcaaa aaatacgccc ggtagtgatc
     3601 ttatttcatt atggtgaaag ttggaacctc ttacgtgccc gatcaa
//