LOCUS       MTK234_026              2094 bp ds-DNA     circular     21-FEB-2019
DEFINITION  .
ACCESSION   <unknown id>                                                       
VERSION     <unknown id>                                                       
KEYWORDS    "sgRNA Dropout"                                                    
FEATURES             Location/Qualifiers
     promoter        complement(1947..2051)
                     /label="CamR Promoter"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     CDS             complement(1287..1946)
                     /label="CamR"
                     /ApEinfo_revcolor=#0000ff
                     /ApEinfo_fwdcolor=#0000ff
     misc_feature    737..1045
                     /label="mU6 promoter (modified)"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    1098..1164
                     /label="modified stem loop"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     rep_origin      complement(join(2052..2094,1..721))
                     /label="ColE1"
                     /ApEinfo_revcolor=#7f7f7f
                     /ApEinfo_fwdcolor=#7f7f7f
     misc_feature    1052..1071
                     /label="GeCKOv2 rg_0111"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    1073..1083
                     /label="modified stem loop"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     protein_bind    complement(1170..1175)
                     /label="BsaI"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     protein_bind    complement(1165..1168)
                     /label="BsaI"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     protein_bind    733..736
                     /label="BsaI"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    1085..1096
                     /label="modified stem loop"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     protein_bind    726..731
                     /label="BsaI"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_recomb     931..964
                     /label="LoxP recombination site"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     terminator      complement(1178..1286)
                     /label="CamR Terminator"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
ORIGIN
        1 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
       61 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
      121 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
      181 tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
      241 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
      301 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
      361 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
      421 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
      481 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
      541 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
      601 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt
      661 ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg
      721 tagtcggtct cAaacggatc cgacgcgcca tctctaggcc cgcgccggcc ccctcgcacg
      781 gacttgtggg agaagctcgg ctactcccct gccccggtta atttgcatat aatatttcct
      841 agtaactata gaggcttaat gtgcgataaa agacagataa tctgttcttt ttaatactag
      901 ctacatttta catgataggc ttggatttct ataacttcgt atagcataca ttatacgaag
      961 ttataaacag cacaaaagga aactcaccct aactgtaaag taattgtgtg ttttgagact
     1021 ataaGtatcc cttggagaaC CAcctTGTTT GACGGAGGCT AAGCGTCGCA AGTTTAAGAG
     1081 CTAAGCTGGA AACAGCATAG CAAGTTTAAA TAAGGCTAGT CCGTTATCAA CTTGAAAAAG
     1141 TGGCACCGAG TCGGTGCTTT TTTTgctgtg agaccagacc aataaaaaac gcccggcggc
     1201 aaccgagcgt tctgaacaaa tccagatgga gttctgaggt cattactgga tctatcaaca
     1261 ggagtccaag cgagctcgat atcaaattac gccccgccct gccactcatc gcagtactgt
     1321 tgtaattcat taagcattct gccgacatgg aagccatcac aaacggcatg atgaacctga
     1381 atcgccagcg gcatcagcac cttgtcgcct tgcgtataat atttgcccat ggtgaaaacg
     1441 ggggcgaaga agttgtccat attggccacg tttaaatcaa aactggtgaa actcacccag
     1501 ggattggctg aaacgaaaaa catattctca ataaaccctt tagggaaata ggccaggttt
     1561 tcaccgtaac acgccacatc ttgcgaatat atgtgtagaa actgccggaa atcgtcgtgg
     1621 tattcactcc agagcgatga aaacgtttca gtttgctcat ggaaaacggt gtaacaaggg
     1681 tgaacactat cccatatcac cagctcaccg tctttcattg ccatacgaaa ttccggatga
     1741 gcattcatca ggcgggcaag aatgtgaata aaggccggat aaaacttgtg cttatttttc
     1801 tttacggtct ttaaaaaggc cgtaatatcc agctgaacgg tctggttata ggtacattga
     1861 gcaactgact gaaatgcctc aaaatgttct ttacgatgcc attgggatat atcaacggtg
     1921 gtatatccag tgattttttt ctccatttta gcttccttag ctcctgaaaa tctcgataac
     1981 tcaaaaaata cgcccggtag tgatcttatt tcattatggt gaaagttgga acctcttacg
     2041 tgcccgatca atcatgacca aaatccctta acgtgagttt tcgttccact gagc
//