LOCUS       MTK234_056-addgene      3034 bp ds-DNA     circular     07-NOV-2019
DEFINITION  .
ACCESSION   <unknown id>                                                       
VERSION     <unknown id>                                                       
KEYWORDS    "sgRNA Dropout"                                                    
FEATURES             Location/Qualifiers
     misc_feature    629..869
                     /label="hu6 Promoter"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     CDS             complement(2119..2778)
                     /label="CamR"
                     /ApEinfo_revcolor=#0000ff
                     /ApEinfo_fwdcolor=#0000ff
     terminator      complement(2010..2118)
                     /label="CamR Terminator"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    1902..1996
                     /label="cr3"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     primer_bind     800..819
                     /label="LKO.1 5'"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     terminator      1767..1895
                     /label="BBa_B0015 Terminator"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     protein_bind    complement(878..883)
                     /label="BsmBI"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     primer_bind     629..649
                     /label="hU6-F"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     protein_bind    1896..1901
                     /label="BsmBI"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    870..877
                     /label="pr"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     protein_bind    625..628
                     /label="BsaI"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     CDS             1050..1766
                     /label="sfGFP"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     rep_origin      complement(join(2884..3034,1..613))
                     /label="ColE1"
                     /ApEinfo_revcolor=#7f7f7f
                     /ApEinfo_fwdcolor=#7f7f7f
     RBS             1014..1049
                     /label="sfGFP Ribosome Binding Site"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     promoter        884..1013
                     /label="BBa_J72163 GlpT Promoter"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     protein_bind    complement(1997..2000)
                     /label="BsaI"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     promoter        complement(2779..2883)
                     /label="CamR Promoter"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     protein_bind    complement(2002..2007)
                     /label="BsaI"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     protein_bind    618..623
                     /label="BsaI"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
ORIGIN
        1 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
       61 accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
      121 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
      181 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
      241 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
      301 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
      361 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
      421 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
      481 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
      541 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc
      601 tgtggataac cgtagtcggt ctcAaacgga gggcctattt cccatgattc cttcatattt
      661 gcatatacga tacaaggctg ttagagagat aattggaatt aatttgactg taaacacaaa
      721 gatattagta caaaatacgt gacgtagaaa gtaataattt cttgggtagt ttgcagtttt
      781 aaaattatgt tttaaaatgg actatcatat gcttaccgta acttgaaagt atttcgattt
      841 cttggcttta tatatcttgt ggaaagccag aaacatggag acggaaagtg aaacgtgatt
      901 tcatgcgtca ttttgaacat tttgtaaatc ttatttaata atgtgtgcgg caattcacat
      961 ttaatttatg aatgttttct taacatcgcg gcaactcaag aaacggcggg ttcggatctt
     1021 agctactaga gaaagaggag aaatactaga tgcgtaaagg cgaagagctg ttcactggtg
     1081 tcgtccctat tctggtggaa ctggatggtg atgtcaacgg tcataagttt tccgtgcgtg
     1141 gcgagggtga aggtgacgca actaatggta aactgacgct gaagttcatc tgtactactg
     1201 gtaaactgcc ggttccttgg ccgactctgg taacgacgct gacttatggt gttcagtgct
     1261 ttgctcgtta tccggaccat atgaagcagc atgacttctt caagtccgcc atgccggaag
     1321 gctatgtgca ggaacgcacg atttccttta aggatgacgg cacgtacaaa acgcgtgcgg
     1381 aagtgaaatt taaaggcgat accctggtaa accgcattga gctgaaaggc attgacttta
     1441 aagaggacgg caatatcctg ggccataagc tggaatacaa ttttaacagc cacaatgttt
     1501 acatcaccgc cgataaacaa aaaaatggca ttaaagcgaa ttttaaaatt cgccacaacg
     1561 tggaggatgg cagcgtgcag ctggctgatc actaccagca aaacactcca atcggtgatg
     1621 gtcctgttct gctgccagac aatcactatc tgagcacgca aagcgttctg tctaaagatc
     1681 cgaacgagaa acgcgatcat atggttctgc tggagttcgt aaccgcagcg ggcatcacgc
     1741 atggtatgga tgaactgtac aaatgaccag gcatcaaata aaacgaaagg ctcagtcgaa
     1801 agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctctact agagtcacac
     1861 tggctcacct tcgggtgggc ctttctgcgt ttatacgtct cgtttcagag ctaagcacaa
     1921 gagtgcatag caagttgaaa taaggctagt ccgtttacaa cttgaaaaag tggcacccga
     1981 gtcgggtgct ttttttgctg tgagaccaga ccaataaaaa acgcccggcg gcaaccgagc
     2041 gttctgaaca aatccagatg gagttctgag gtcattactg gatctatcaa caggagtcca
     2101 agcgagctcg atatcaaatt acgccccgcc ctgccactca tcgcagtact gttgtaattc
     2161 attaagcatt ctgccgacat ggaagccatc acaaacggca tgatgaacct gaatcgccag
     2221 cggcatcagc accttgtcgc cttgcgtata atatttgccc atggtgaaaa cgggggcgaa
     2281 gaagttgtcc atattggcca cgtttaaatc aaaactggtg aaactcaccc agggattggc
     2341 tgaaacgaaa aacatattct caataaaccc tttagggaaa taggccaggt tttcaccgta
     2401 acacgccaca tcttgcgaat atatgtgtag aaactgccgg aaatcgtcgt ggtattcact
     2461 ccagagcgat gaaaacgttt cagtttgctc atggaaaacg gtgtaacaag ggtgaacact
     2521 atcccatatc accagctcac cgtctttcat tgccatacga aattccggat gagcattcat
     2581 caggcgggca agaatgtgaa taaaggccgg ataaaacttg tgcttatttt tctttacggt
     2641 ctttaaaaag gccgtaatat ccagctgaac ggtctggtta taggtacatt gagcaactga
     2701 ctgaaatgcc tcaaaatgtt ctttacgatg ccattgggat atatcaacgg tggtatatcc
     2761 agtgattttt ttctccattt tagcttcctt agctcctgaa aatctcgata actcaaaaaa
     2821 tacgcccggt agtgatctta tttcattatg gtgaaagttg gaacctctta cgtgcccgat
     2881 caatcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     2941 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     3001 caaaaaaacc accgctacca gcggtggttt gttt
//