LOCUS       MTK0_001                3672 bp ds-DNA     circular     20-FEB-2019
DEFINITION  .
FEATURES             Location/Qualifiers
     protein_bind    1358..1363
                     /label="BsmBI"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    1365..1389
                     /label="Con1 scar"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    1215..1357
                     /label="Con1"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     rep_origin      complement(424..1187)
                     /label="ColE1"
                     /ApEinfo_revcolor=#7f7f7f
                     /ApEinfo_fwdcolor=#7f7f7f
     promoter        1714..1843
                     /label="BBa_J72163 GlpT Promoter"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     terminator      complement(3010..3106)
                     /label="AmpR Terminator"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     RBS             1844..1879
                     /label="sfGFP Ribosome Binding Site"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     promoter        complement(293..423)
                     /label="AmpR Promoter"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     protein_bind    complement(2834..2839)
                     /label="BsmBI"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    2758..2824
                     /label="modified stem loop"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    1394..1707
                     /label="mU6 promoter (modified)"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_recomb     1588..1621
                     /label="LoxP recombination site"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    2732..2743
                     /label="modified stem loop"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     CDS             1880..2596
                     /label="sfGFP"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    2840..2982
                     /label="ConE"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     CDS             complement(join(3107..3672,1..292))
                     /label="AmpR"
                     /ApEinfo_revcolor=#9f4240
                     /ApEinfo_fwdcolor=#9f4240
     protein_bind    1365..1368
                     /label="BsmBI"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     protein_bind    complement(1708..1713)
                     /label="BsmBI"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     protein_bind    2726..2731
                     /label="BsmBI"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     terminator      2597..2725
                     /label="BBa_B0015 Terminator"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     protein_bind    complement(2829..2832)
                     /label="BsmBI"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    2745..2756
                     /label="modified stem loop"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
ORIGIN
        1 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata
       61 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
      121 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
      181 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
      241 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
      301 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
      361 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
      421 ctgtcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
      481 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
      541 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
      601 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
      661 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
      721 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
      781 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
      841 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
      901 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
      961 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     1021 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     1081 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     1141 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtgcg gccgcccctg
     1201 aattcgcatc tagaacgggg tcatcacggc tcatcatgcg ccaaacaaat gtgtgcaata
     1261 cacgctcgga tgactgcatg atgaccgcac tgactgggga cagcagatcc acctaagcct
     1321 gtgagagaag cagacacccg acagatcaag gcagttacgt ctcaccaaac cagatgtcaa
     1381 cacagctaca acggatccga cgcgccatct ctaggcccgc gccggccccc tcgcacggac
     1441 ttgtgggaga agctcggcta ctcccctgcc ccggttaatt tgcatataat atttcctagt
     1501 aactatagag gcttaatgtg cgataaaaga cagataatct gttcttttta atactagcta
     1561 cattttacat gataggcttg gatttctata acttcgtata gcatacatta tacgaagtta
     1621 taaacagcac aaaaggaaac tcaccctaac tgtaaagtaa ttgtgtgttt tgagactata
     1681 aGtatccctt ggagaaCCAc ctTGTTGgag acggaaagtg aaacgtgatt tcatgcgtca
     1741 ttttgaacat tttgtaaatc ttatttaata atgtgtgcgg caattcacat ttaatttatg
     1801 aatgttttct taacatcgcg gcaactcaag aaacggcagg ttcggatctt agctactaga
     1861 gaaagaggag aaatactaga tgcgtaaagg cgaagagctg ttcactggtg tcgtccctat
     1921 tctggtggaa ctggatggtg atgtcaacgg tcataagttt tccgtgcgtg gcgagggtga
     1981 aggtgacgca actaatggta aactgacgct gaagttcatc tgtactactg gtaaactgcc
     2041 ggttccttgg ccgactctgg taacgacgct gacttatggt gttcagtgct ttgctcgtta
     2101 tccggaccat atgaagcagc atgacttctt caagtccgcc atgccggaag gctatgtgca
     2161 ggaacgcacg atttccttta aggatgacgg cacgtacaaa acgcgtgcgg aagtgaaatt
     2221 tgaaggcgat accctggtaa accgcattga gctgaaaggc attgacttta aagaggacgg
     2281 caatatcctg ggccataagc tggaatacaa ttttaacagc cacaatgttt acatcaccgc
     2341 cgataaacaa aaaaatggca ttaaagcgaa ttttaaaatt cgccacaacg tggaggatgg
     2401 cagcgtgcag ctggctgatc actaccagca aaacactcca atcggtgatg gtcctgttct
     2461 gctgccagac aatcactatc tgagcacgca aagcgttctg tctaaagatc cgaacgagaa
     2521 acgcgatcat atggttctgc tggagttcgt aaccgcagcg ggcatcacgc atggtatgga
     2581 tgaactgtac aaatgaccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc
     2641 tttcgtttta tctgttgttt gtcggtgaac gctctctact agagtcacac tggctcacct
     2701 tcgggtgggc ctttctgcgt ttatacgtct cGTTTAAGAG CTAAGCTGGA AACAGCATAG
     2761 CAAGTTTAAA TAAGGCTAGT CCGTTATCAA CTTGAAAAAG TGGCACCGAG TCGGTGCTTT
     2821 TTTTgctgag catgagacgg aaatctgctc gtcagtggtg ctcacactga cgaatcatgt
     2881 acagatcata ccgatgactg cctggcgact cacaactaag caagacagcc ggaaccagcg
     2941 ccggcgaaca ccactgcata tatggcatat cacaacagtc caactagtgc actgcagtac
     3001 agcggccgcg attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
     3061 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
     3121 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
     3181 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
     3241 cgcgggaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
     3301 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
     3361 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta
     3421 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
     3481 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
     3541 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
     3601 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
     3661 caaccaagtc at
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