LOCUS       MTK0_014                4481 bp ds-DNA     circular     21-FEB-2019
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    2143..2422
                     /label="HS4"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     terminator      complement(3143..3169)
                     /label="KanR Terminator"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    1806..1830
                     /label="ConE scar"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    2442..2487
                     /label="attB (BxBI)"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     protein_bind    1799..1804
                     /label="BsmBI"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     rep_origin      complement(join(4134..4481,1..416))
                     /label="ColE1"
                     /ApEinfo_revcolor=#7f7f7f
                     /ApEinfo_fwdcolor=#7f7f7f
     misc_feature    1656..1798
                     /label="ConE'"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     promoter        complement(3986..4133)
                     /label="KanR Promoter"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     protein_bind    complement(482..485)
                     /label="BsmBI"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     protein_bind    complement(487..492)
                     /label="BsmBI"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    429..462
                     /label="LoxP"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     CDS             complement(3170..3985)
                     /label="KanR"
                     /ApEinfo_revcolor=#008000
                     /ApEinfo_fwdcolor=#008000
     Terminator      2897..3130
                     /label="BGH-PA"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     protein_bind    1806..1809
                     /label="BsmBI"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     CDS             806..1522
                     /label="sfGFP"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     RBS             770..805
                     /label="sfGFP Ribosome Binding Site"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    493..635
                     /label="ConS'"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    1860..2139
                     /label="HS4"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     terminator      1523..1651
                     /label="BBa_B0015 Terminator"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     CDS             2495..2888
                     /label="Blast"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     promoter        640..769
                     /label="BBa_J72163 GlpT Promoter"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
ORIGIN
        1 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
       61 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
      121 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
      181 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
      241 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
      301 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
      361 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtgcgg
      421 ccgccaatat aacttcgtat aATGTATGCt atacgaagtt atCCCTgaat tcgcatctag
      481 actgatgaga cgtggtagag ccacaaacag ccggtacaag caacgatctc caggaccatc
      541 tgaatcatgc gcggatgaca cgaactcacg acggcgatca cagacattaa cccacagtac
      601 agacactgcg acaacgtggc aattcgtcgc aatacaacgg aaagtgaaac gtgatttcat
      661 gcgtcatttt gaacattttg taaatcttat ttaataatgt gtgcggcaat tcacatttaa
      721 tttatgaatg ttttcttaac atcgcggcaa ctcaagaaac ggcaggttcg gatcttagct
      781 actagagaaa gaggagaaat actagatgcg taaaggcgaa gagctgttca ctggtgtcgt
      841 ccctattctg gtggaactgg atggtgatgt caacggtcat aagttttccg tgcgtggcga
      901 gggtgaaggt gacgcaacta atggtaaact gacgctgaag ttcatctgta ctactggtaa
      961 actgccggtt ccttggccga ctctggtaac gacgctgact tatggtgttc agtgctttgc
     1021 tcgttatccg gaccatatga agcagcatga cttcttcaag tccgccatgc cggaaggcta
     1081 tgtgcaggaa cgcacgattt cctttaagga tgacggcacg tacaaaacgc gtgcggaagt
     1141 gaaatttgaa ggcgataccc tggtaaaccg cattgagctg aaaggcattg actttaaaga
     1201 ggacggcaat atcctgggcc ataagctgga atacaatttt aacagccaca atgtttacat
     1261 caccgccgat aaacaaaaaa atggcattaa agcgaatttt aaaattcgcc acaacgtgga
     1321 ggatggcagc gtgcagctgg ctgatcacta ccagcaaaac actccaatcg gtgatggtcc
     1381 tgttctgctg ccagacaatc actatctgag cacgcaaagc gttctgtcta aagatccgaa
     1441 cgagaaacgc gatcatatgg ttctgctgga gttcgtaacc gcagcgggca tcacgcatgg
     1501 tatggatgaa ctgtacaaat gaccaggcat caaataaaac gaaaggctca gtcgaaagac
     1561 tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tctactagag tcacactggc
     1621 tcaccttcgg gtgggccttt ctgcgtttat agctggaaat ctgctcgtca gtggtgctca
     1681 cactgacgaa tcatgtacag atcataccga tgactgcctg gcgactcaca actaagcaag
     1741 acagccggaa ccagcgccgg cgaacaccac tgcatatatg gcatatcaca acagtccacg
     1801 tctcaagcag ttacagagat gttacgaacc actagtgcac tgcagATCGA ATTCGAGTTG
     1861 GCGCGCCTGT CATTCTAAAT CTCTCTTTCA GCCTAAAGCT TTTTCCCCGT ATCCCCCCAG
     1921 GTGTCTGCAG GCTCAAAGAG CAGCGAGAAG CGTTCAGAGG AAAGCGATCC CGTGCCACCT
     1981 TCCCCGTGCC CGGGCTGTCC CCGCACGCTG CCGGCTCGGG GATGCGGGGG GAGCGCCGGA
     2041 CCGGAGCGGA GCCCCGGGCG GCTCGCTGCT GCCCCCTAGC GGGGGAGGGA CGTAATTACA
     2101 TCCCTGGGGG CTTTGGGAGG GGGCTGTCCC CGTGAGCTCC CAGGCGCGCC TGTCATTCTA
     2161 AATCTCTCTT TCAGCCTAAA GCTTTTTCCC CGTATCCCCC CAGGTGTCTG CAGGCTCAAA
     2221 GAGCAGCGAG AAGCGTTCAG AGGAAAGCGA TCCCGTGCCA CCTTCCCCGT GCCCGGGCTG
     2281 TCCCCGCACG CTGCCGGCTC GGGGATGCGG GGGGAGCGCC GGACCGGAGC GGAGCCCCGG
     2341 GCGGCTCGCT GCTGCCCCCT AGCGGGGGAG GGACGTAATT ACATCCCTGG GGGCTTTGGG
     2401 GGGGGGCTGT CCCCGTGAGC TCAATAAAAG AGCCCACtac aggccggctt gtcgacgacg
     2461 gcggtctccg tcgtcaggat catccggGAG TATGGCCAAG CCTTTGTCTC AAGAAGAATC
     2521 CACCCTCATT GAAAGAGCAA CGGCTACAAT CAACAGCATC CCCATCTCTG AAGACTACAG
     2581 CGTCGCCAGC GCAGCTCTCT CTAGCGACGG CCGCATCTTC ACTGGTGTCA ATGTATATCA
     2641 TTTTACTGGG GGACCTTGTG CAGAACTCGT GGTGCTGGGC ACTGCTGCTG CTGCGGCAGC
     2701 TGGCAACCTG ACTTGTATCG TCGCGATCGG AAATGAGAAC AGGGGCATCT TGAGCCCCTG
     2761 CGGACGGTGC CGACAGGTGC TTCTCGATCT GCATCCTGGG ATCAAAGCCA TAGTGAAGGA
     2821 CAGTGATGGA CAGCCGACGG CAGTTGGGAT TCGTGAATTG CTGCCCTCTG GTTATGTGTG
     2881 GGAGGGCTAA CTCGAGCGAC GCCTCGACTG TGCCTTCTAG TTGCCAGCCA TCTGTTGTTT
     2941 GCCCCTCCCC CGTGCCTTCC TTGACCCTGG AAGGTGCCAC TCCCACTGTC CTTTCCTAAT
     3001 AAAATGAGGA AATTGCATCG CATTGTCTGA GTAGGTGTCA TTCTATTCTG GGGGGTGGGG
     3061 TGGGGCAGGA CAGCAAGGGG GAGGATTGGG AAGACAATAG CAGGCATGCT GGGGATGCGG
     3121 TGGGCTCTAT CCGAgcggcc gcgtgttaca accaattaac caattctgat tagaaaaact
     3181 catcgagcat caaatgaaac tgcaatttat tcatatcagg attatcaata ccatattttt
     3241 gaaaaagccg tttctgtaat gaaggagaaa actcaccgag gcagttccat aggatggcaa
     3301 gatcctggta tcggtctgcg attccgactc gtccaacatc aatacaacct attaatttcc
     3361 cctcgtcaaa aataaggtta tcaagtgaga aatcaccatg agtgacgact gaatccggtg
     3421 agaatggcaa aagcttatgc atttctttcc agacttgttc aacaggccag ccattacgct
     3481 cgtcatcaaa atcactcgca tcaaccaaac cgttattcat tcgtgattgc gcctgagcga
     3541 ggcgaaatac gcgatcgctg ttaaaaggac aattacaaac aggaatcgaa tgcaaccggc
     3601 gcaggaacac tgccagcgca tcaacaatat tttcacctga atcaggatat tcttctaata
     3661 cctggaatgc tgttttcccg gggatcgcag tggtgagtaa ccatgcatca tcaggagtac
     3721 ggataaaatg cttgatggtc ggaagaggca taaattccgt cagccagttt agtctgacca
     3781 tctcatctgt aacatcattg gcaacgctac ctttgccatg tttcagaaac aactctggcg
     3841 catcgggctt cccatacaat cgatagattg tcgcacctga ttgcccgaca ttatcgcgag
     3901 cccatttata cccatataaa tcagcatcca tgttggaatt taatcgcggc ctggagcaag
     3961 acgtttcccg ttgaatatgg ctcataacac cccttgtatt actgtttatg taagcagaca
     4021 gttttattgt tcatgatgat atatttttat cttgtgcaat gtaacatcag agattttgag
     4081 acacaacgtg gctttgttga ataaatcgaa cttttgctga gttgaaggat cagtcatgac
     4141 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     4201 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     4261 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     4321 aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg
     4381 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     4441 agtggctgct gccagtggcg ataagtcgtg tcttaccggg t
//