LOCUS       MTK234_009              2094 bp ds-DNA     circular     22-FEB-2019
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_recomb     1025..1058
                     /label="LoxP recombination site"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     rep_origin      complement(52..815)
                     /label="ColE1"
                     /ApEinfo_revcolor=#7f7f7f
                     /ApEinfo_fwdcolor=#7f7f7f
     protein_bind    820..825
                     /label="BsaI"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    1179..1190
                     /label="modified stem loop"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    1146..1165
                     /label="C120 Guide"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     protein_bind    827..830
                     /label="BsaI"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     promoter        complement(join(2041..2094,1..51))
                     /label="CamR Promoter"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     protein_bind    complement(1264..1269)
                     /label="BsaI"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     protein_bind    complement(1259..1262)
                     /label="BsaI"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     CDS             complement(1381..2040)
                     /label="CamR"
                     /ApEinfo_revcolor=#0000ff
                     /ApEinfo_fwdcolor=#0000ff
     misc_feature    1192..1258
                     /label="modified stem loop"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     terminator      complement(1272..1380)
                     /label="CamR Terminator"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
ORIGIN
        1 tgatcttatt tcattatggt gaaagttgga acctcttacg tgcccgatca atcatgacca
       61 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
      121 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
      181 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
      241 ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc
      301 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
      361 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
      421 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
      481 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
      541 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
      601 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
      661 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
      721 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
      781 ttcctgcgtt atcccctgat tctgtggata accgtagtcg gtctcAaacg gatccgacgc
      841 gccatctcta ggcccgcgcc ggccccctcg cacggacttg tgggagaagc tcggctactc
      901 ccctgccccg gttaatttgc atataatatt tcctagtaac tatagaggct taatgtgcga
      961 taaaagacag ataatctgtt ctttttaata ctagctacat tttacatgat aggcttggat
     1021 ttctataact tcgtatagca tacattatac gaagttataa acagcacaaa aggaaactca
     1081 ccctaactgt aaagtaattg tgtgttttga gactataaGt atcccttgga gaaCCAcctT
     1141 GTTtGGCCTT TAGTCCATGC GTTATGTTTA AGAGCTAAGC TGGAAACAGC ATAGCAAGTT
     1201 TAAATAAGGC TAGTCCGTTA TCAACTTGAA AAAGTGGCAC CGAGTCGGTG CTTTTTTTgc
     1261 tgtgagacca gaccaataaa aaacgcccgg cggcaaccga gcgttctgaa caaatccaga
     1321 tggagttctg aggtcattac tggatctatc aacaggagtc caagcgagct cgatatcaaa
     1381 ttacgccccg ccctgccact catcgcagta ctgttgtaat tcattaagca ttctgccgac
     1441 atggaagcca tcacaaacgg catgatgaac ctgaatcgcc agcggcatca gcaccttgtc
     1501 gccttgcgta taatatttgc ccatggtgaa aacgggggcg aagaagttgt ccatattggc
     1561 cacgtttaaa tcaaaactgg tgaaactcac ccagggattg gctgaaacga aaaacatatt
     1621 ctcaataaac cctttaggga aataggccag gttttcaccg taacacgcca catcttgcga
     1681 atatatgtgt agaaactgcc ggaaatcgtc gtggtattca ctccagagcg atgaaaacgt
     1741 ttcagtttgc tcatggaaaa cggtgtaaca agggtgaaca ctatcccata tcaccagctc
     1801 accgtctttc attgccatac gaaattccgg atgagcattc atcaggcggg caagaatgtg
     1861 aataaaggcc ggataaaact tgtgcttatt tttctttacg gtctttaaaa aggccgtaat
     1921 atccagctga acggtctggt tataggtaca ttgagcaact gactgaaatg cctcaaaatg
     1981 ttctttacga tgccattggg atatatcaac ggtggtatat ccagtgattt ttttctccat
     2041 tttagcttcc ttagctcctg aaaatctcga taactcaaaa aatacgcccg gtag
//