LOCUS       MTK0_031                6706 bp ds-DNA     circular     22-FEB-2019
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    1886..2028
                     /label="ConS"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     primer          4309..4328
                     /label="pCAG_F_primer"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     CDS             5556..6263
                     /label="mRuby2"
                     /ApEinfo_revcolor=#ff454e
                     /ApEinfo_fwdcolor=#ff454e
     protein_bind    complement(6534..6537)
                     /label="BsmBI"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     CDS             complement(106..963)
                     /label="AmpR"
                     /ApEinfo_revcolor=#9f4240
                     /ApEinfo_fwdcolor=#9f4240
     misc_feature    6545..6687
                     /label="ConE"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     terminator      complement(9..105)
                     /label="AmpR Terminator"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     Terminator      6400..6529
                     /label="SV40-PA"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    2075..2354
                     /label="HS4"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     CDS             4422..5444
                     /label="HPH (Hygromycin Phosphotransferase)"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     misc_feature    2358..2637
                     /label="HS4"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     protein_bind    complement(6539..6544)
                     /label="BsmBI"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     protein_bind    2029..2034
                     /label="BsmBI"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     rep_origin      complement(1095..1858)
                     /label="ColE1"
                     /ApEinfo_revcolor=#7f7f7f
                     /ApEinfo_fwdcolor=#7f7f7f
     misc_feature    6270..6386
                     /label="d4PEST (MODC)"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     regulatory      2712..2999
                     /label="CAG_enhancer"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    2653..2686
                     /label="LoxP"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     sig_peptide     5520..5549
                     /label="NLS_(zhang)"
                     /ApEinfo_revcolor=#ff0f00
                     /ApEinfo_fwdcolor=#ff0f00
     misc_feature    2036..2060
                     /label="ConS scar"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    2691..4355
                     /label="CAG promotor"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     promoter        complement(964..1094)
                     /label="AmpR Promoter"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     protein_bind    2036..2039
                     /label="BsmBI"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_signal     5451..5513
                     /label="T2A"
                     /ApEinfo_revcolor=#0080ff
                     /ApEinfo_fwdcolor=#0080ff
ORIGIN
        1 gcggccgcga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
       61 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
      121 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
      181 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
      241 gcgggaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
      301 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
      361 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
      421 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
      481 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
      541 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
      601 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
      661 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
      721 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
      781 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
      841 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
      901 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
      961 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
     1021 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
     1081 aaaagtgcca cctgtcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
     1141 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     1201 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     1261 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct
     1321 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     1381 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     1441 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     1501 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     1561 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
     1621 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     1681 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     1741 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
     1801 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtgc
     1861 ggccgcccct gaattcgcat ctagatggta gagccacaaa cagccggtac aagcaacgat
     1921 ctccaggacc atctgaatca tgcgcggatg acacgaactc acgacggcga tcacagacat
     1981 taacccacag tacagacact gcgacaacgt ggcaattcgt cgcaataccg tctcactgaa
     2041 ctggccgata attgcagacg ATCGAATTCG AGTTGGCGCG CCTGTCATTC TAAATCTCTC
     2101 TTTCAGCCTA AAGCTTTTTC CCCGTATCCC CCCAGGTGTC TGCAGGCTCA AAGAGCAGCG
     2161 AGAAGCGTTC AGAGGAAAGC GATCCCGTGC CACCTTCCCC GTGCCCGGGC TGTCCCCGCA
     2221 CGCTGCCGGC TCGGGGATGC GGGGGGAGCG CCGGACCGGA GCGGAGCCCC GGGCGGCTCG
     2281 CTGCTGCCCC CTAGCGGGGG AGGGACGTAA TTACATCCCT GGGGGCTTTG GGAGGGGGCT
     2341 GTCCCCGTGA GCTCCCAGGC GCGCCTGTCA TTCTAAATCT CTCTTTCAGC CTAAAGCTTT
     2401 TTCCCCGTAT CCCCCCAGGT GTCTGCAGGC TCAAAGAGCA GCGAGAAGCG TTCAGAGGAA
     2461 AGCGATCCCG TGCCACCTTC CCCGTGCCCG GGCTGTCCCC GCACGCTGCC GGCTCGGGGA
     2521 TGCGGGGGGA GCGCCGGACC GGAGCGGAGC CCCGGGCGGC TCGCTGCTGC CCCCTAGCGG
     2581 GGGAGGGACG TAATTACATC CCTGGGGGCT TTGGGGGGGG GCTGTCCCCG TGAGCTCAAT
     2641 AAAAGAGCCC ACataacttc gtataATGTA TGCtatacga agttataacg CCATATATGG
     2701 AGTTCCGCGT TACATAACTT ACGGTAAATG GCCCGCCTGG CTGACCGCCC AACGACCCCC
     2761 GCCCATTGAC GTCAATAATG ACGTATGTTC CCATAGTAAC GCCAATAGGG ACTTTCCATT
     2821 GACGTCAATG GGTGGAGTAT TTACGGTAAA CTGCCCACTT GGCAGTACAT CAAGTGTATC
     2881 ATATGCCAAG TACGCCCCCT ATTGACGTCA ATGACGGTAA ATGGCCCGCC TGGCATTATG
     2941 CCCAGTACAT GACCTTATGG GACTTTCCTA CTTGGCAGTA CATCTACGTA TTAGTCATCG
     3001 CTATTACCAT GGTCGAGGTG AGCCCCACGT TCTGCTTCAC TCTCCCCATC TCCCCCCCCT
     3061 CCCCACCCCC AATTTTGTAT TTATTTATTT TTTAATTATT TTGTGCAGCG ATGGGGGCGG
     3121 GGGGGGGGGG GGCGCGCGCC AGGCGGGGCG GGGCGGGGCG AGGGGCGGGG CGGGGCGAGG
     3181 CGGAGAGGTG CGGCGGCAGC CAATCAGAGC GGCGCGCTCC GAAAGTTTCC TTTTATGGCG
     3241 AGGCGGCGGC GGCGGCGGCC CTATAAAAAG CGAAGCGCGC GGCGGGCGGG AGTCGCTGCG
     3301 TTGCCTTCGC CCCGTGCCCC GCTCCGCGCC GCCTCGCGCC GCCCGCCCCG GCTCTGACTG
     3361 ACCGCGTTAC TCCCACAGGT GAGCGGGCGG GACGGCCCTT CTCCTCCGGG CTGTAATTAG
     3421 CGCTTGGTTT AATGACGGCT CGTTTCTTTT CTGTGGCTGC GTGAAAGCCT TAAAGGGCTC
     3481 CGGGAGGGCC CTTTGTGCGG GGGGGAGCGG CTCGGGGGGT GCGTGCGTGT GTGTGTGCGT
     3541 GGGGAGCGCC GCGTGCGGCC CGCGCTGCCC GGCGGCTGTG AGCGCTGCGG GCGCGGCGCG
     3601 GGGCTTTGTG CGCTCCGCGT GTGCGCGAGG GGAGCGCGGC CGGGGGCGGT GCCCCGCGGT
     3661 GCGGGGGGGC TGCGAGGGGA ACAAAGGCTG CGTGCGGGGT GTGTGCGTGG GGGGGTGAGC
     3721 AGGGGGTGTG GGCGCGGCGG TCGGGCTGTA ACCCCCCCCT GCACCCCCCT CCCCGAGTTG
     3781 CTGAGCACGG CCCGGCTTCG GGTGCGGGGC TCCGTaCGGG GCGTGGCGCG GGGCTCGCCG
     3841 TGCCGGGCGG GGGGTGGCGG CAGGTGGGGG TGCCGGGCGG GGCGGGGCCG CCTCGGGCCG
     3901 GGGAGGGCTC GGGGGAGGGG CGCGGCGGcC CCCGGAGtGC CGGCGGCTGT CGAGGCGCGG
     3961 CGAGCCGCAG CCATTGCCTT TTATGGTAAT CGTGCGAGAG GGCGCAGGGA CTTCCTTTGT
     4021 CCCAAATCTG TGCGGAGCCG AAATCTGGGA GGCGCCGCCG CACCCCCTCT AGCGGGCGCg
     4081 GGGCGAAGCG GTGCGGCGCC GGCAGGAAGG AAATGGGCGG GGAGGGCCTT CGTGCGTCGC
     4141 CGCGCCGCCG TCCCCTTCTC CcTCTCCAGC CTCGGGGCTG TCCGCgGGGG GACGGCTGCC
     4201 TTCGGGGGGG ACGGGGCAGG GCGGGGTTCG GCTTCTGGCG TGTGACCGGC GGCTCTAGAG
     4261 CCTCTGCTAA CCATGTTCAT GCCTTCTTCT TTTTCCTACA GCTCCTGGGC AACGTGCTGG
     4321 TTATTGTGCT GTCTCATCAT TTTGGCAAAG aattcgccac TATGGTGGTT TGTCTGGTCA
     4381 ACCACCgcgg tctcAGTGGT GTACGGTACA AACCAGGTTC Tggtaaaaag cctgaactca
     4441 ccgcgacgtc tgtcgagaag tttctgatcg aaaagttcga cagcgtgtcc gacctgatgc
     4501 agctctcgga gggcgaagaa tctcgtgctt tcagcttcga tgtaggaggg cgtggatatg
     4561 tcctgcgggt aaatagctgc gccgatggtt tctacaaaga tcgttatgtt tatcggcact
     4621 ttgcatcggc cgcgctcccg attccggaag tgcttgacat tggggaattt agcgagagcc
     4681 tgacctattg catctcccgc cgtgcacagg gtgtcacgtt gcaagacctg cctgaaaccg
     4741 aactgcccgc tgttctgcaa ccggtcgcgg aggccatgga tgcgatcgct gcggccgatc
     4801 ttagccagac gagcgggttc ggcccattcg gaccgcaagg aatcggtcaa tacactacat
     4861 ggcgtgattt catatgcgcg attgctgatc cccatgtgta tcactggcaa actgtgatgg
     4921 acgacaccgt cagtgcgtcc gtcgcgcagg ctctcgatga gctgatgctt tgggccgagg
     4981 actgccccga agtccggcac ctcgtgcacg cggatttcgg ctccaacaat gtcctgacgg
     5041 acaatggccg cataacagcg gtcattgact ggagcgaggc gatgttcggg gattcccaat
     5101 acgaggtcgc caacatcttc ttctggaggc cgtggttggc ttgtatggag cagcagacgc
     5161 gctacttcga gcggaggcat ccggagcttg caggatcgcc gcggctccgg gcgtatatgc
     5221 tccgcattgg tcttgaccaa ctctatcaga gcttggttga cggcaatttc gatgatgcag
     5281 cttgggcgca gggtcgatgc gacgcaatcg tccgatccgg agccgggact gtcgggcgta
     5341 cacaaatcgc ccgcagaagc gcggccgtct ggaccgatgg ctgtgtagaa gtactcgccg
     5401 atagtggaaa ccgacgcccc agcactcgtc cgagggcaaa ggaaGGATCC GGAAGCGGAG
     5461 AGGGCAGAGG AAGTCTGCTA ACATGCGGTG ACGTCGAGGA GAATCCTGGA CCTGGATCCC
     5521 CCAAGAAGAA GAGGAAGGTG GAGGACGCCG GATCCgtgtc caaaggagag gagttaatca
     5581 aggaaaacat gagaatgaaa gttgtcatgg agggctccgt taatggtcac caattcaagt
     5641 gtacagggga aggtgaaggt aatccttaca tgggtacaca aactatgaga attaaagtaa
     5701 ttgaaggcgg accactacca tttgcatttg acattctggc aacgtcattc atgtacggat
     5761 cacgaacttt catcaagtac cctaaaggta taccagactt tttcaagcaa tcttttccag
     5821 agggttttac atgggaaagg gttacaagat acgaagatgg gggtgtcgtc acagttatgc
     5881 aagatacttc attagaagat ggctgccttg tctatcatgt gcaagtaaga ggggtgaatt
     5941 ttccttctaa cggacctgtg atgcagaaaa agaccaaagg ttgggaacca aatactgaaa
     6001 tgatgtaccc agctgatgga ggtttgagag gctacacaca catggcgctt aaagttgatg
     6061 gtggaggtca tttgtcttgt agttttgtta ccacttatcg ttctaaaaag actgttggca
     6121 atatcaaaat gccaggaata catgctgtag accacagact agaaagactc gaagagagcg
     6181 ataacgaaat gttcgttgta cagagagagc atgccgtagc caaatttgct ggcttaggcg
     6241 gtggtatgga tgaattgtat aagggaTCCC ATGGCTTCCC GCCGGAGGTG GAGGAGCAGG
     6301 ATGATGGCGC GCTGCCCATG TCTTGTGCCC AGGAGAGCGG GATGGACCGT CACCCTGCAG
     6361 CCTGTGCTTC TGCTAGGATC AATGTGTAAC TCGAGTGGCg atccagacat gataagatac
     6421 attgatgagt ttggacaaac cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa
     6481 atttgtgatg ctattgcttt atttgtaacc attataagct gcaataaacG CTGagcatga
     6541 gacggaaatc tgctcgtcag tggtgctcac actgacgaat catgtacaga tcataccgat
     6601 gactgcctgg cgactcacaa ctaagcaaga cagccggaac cagcgccggc gaacaccact
     6661 gcatatatgg catatcacaa cagtccaact agtgcactgc agtaca
//