LOCUS       MTK0_034                7885 bp ds-DNA     circular     22-FEB-2019
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    2198..2231
                     /label="FRT"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     promoter        complement(816..963)
                     /label="KanR Promoter"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     sig_peptide     5704..5733
                     /label="NLS_(zhang)"
                     /ApEinfo_revcolor=#ff0f00
                     /ApEinfo_fwdcolor=#ff0f00
     misc_feature    6772..7051
                     /label="HS4"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     primer          4505..4524
                     /label="pCAG_F_primer"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    2271..2550
                     /label="HS4"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     regulatory      2908..3195
                     /label="CAG_enhancer"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    2887..4551
                     /label="CAG promotor"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     misc_signal     5635..5697
                     /label="T2A"
                     /ApEinfo_revcolor=#0080ff
                     /ApEinfo_fwdcolor=#0080ff
     terminator      complement(7858..7884)
                     /label="KanR Terminator"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     Terminator      6584..6713
                     /label="SV40-PA"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     CDS             5740..6447
                     /label="mRuby2"
                     /ApEinfo_revcolor=#ff454e
                     /ApEinfo_fwdcolor=#ff454e
     misc_feature    6454..6570
                     /label="d4PEST (MODC)"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     CDS             4606..5628
                     /label="HPH (Hygromycin Phosphotransferase)"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     CDS             complement(join(7885..7885,1..815))
                     /label="KanR"
                     /ApEinfo_revcolor=#008000
                     /ApEinfo_fwdcolor=#008000
     misc_feature    7354..7387
                     /label="FRT"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    4561..4600
                     /label="attP (phiC31)"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     misc_feature    2554..2833
                     /label="HS4"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     rep_origin      complement(964..1727)
                     /label="ColE1"
                     /ApEinfo_revcolor=#7f7f7f
                     /ApEinfo_fwdcolor=#7f7f7f
     misc_feature    2849..2882
                     /label="LoxP"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    7055..7334
                     /label="HS4"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
ORIGIN
        1 tagaaaaact catcgagcat caaatgaaac tgcaatttat tcatatcagg attatcaata
       61 ccatattttt gaaaaagccg tttctgtaat gaaggagaaa actcaccgag gcagttccat
      121 aggatggcaa gatcctggta tcggtctgcg attccgactc gtccaacatc aatacaacct
      181 attaatttcc cctcgtcaaa aataaggtta tcaagtgaga aatcaccatg agtgacgact
      241 gaatccggtg agaatggcaa aagcttatgc atttctttcc agacttgttc aacaggccag
      301 ccattacgct cgtcatcaaa atcactcgca tcaaccaaac cgttattcat tcgtgattgc
      361 gcctgagcga ggcgaaatac gcgatcgctg ttaaaaggac aattacaaac aggaatcgaa
      421 tgcaaccggc gcaggaacac tgccagcgca tcaacaatat tttcacctga atcaggatat
      481 tcttctaata cctggaatgc tgttttcccg gggatcgcag tggtgagtaa ccatgcatca
      541 tcaggagtac ggataaaatg cttgatggtc ggaagaggca taaattccgt cagccagttt
      601 agtctgacca tctcatctgt aacatcattg gcaacgctac ctttgccatg tttcagaaac
      661 aactctggcg catcgggctt cccatacaat cgatagattg tcgcacctga ttgcccgaca
      721 ttatcgcgag cccatttata cccatataaa tcagcatcca tgttggaatt taatcgcggc
      781 ctggagcaag acgtttcccg ttgaatatgg ctcataacac cccttgtatt actgtttatg
      841 taagcagaca gttttattgt tcatgatgat atatttttat cttgtgcaat gtaacatcag
      901 agattttgag acacaacgtg gctttgttga ataaatcgaa cttttgctga gttgaaggat
      961 cagtcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     1021 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     1081 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     1141 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
     1201 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     1261 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     1321 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     1381 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     1441 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     1501 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     1561 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     1621 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     1681 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtgcg gccgccaatC
     1741 ATGGTCATAG CTGTTTCCGG GGAGAGAGGG TGAGGGAGGG AAGCACTGGT TTCTCAAGCA
     1801 AAAGCTAAAA TTTTTCTATT AAGATTTAAC CTGATGCTAC ACTTTGGTGG TGCAGCAAGG
     1861 GTCTAAAATG GTATAAAACT CAGGTGATCA TGCTTTATGT CTGTCTCTAG AAAAATGCTC
     1921 CAAAAATGAT AAGTAGTGAT AATCCGCAGT CTCGTTGCAT AAAATCAGCG CCAGGTGAAT
     1981 GACTAAGCTC CATTTCCCTA CCCCACCCTT ATTACAATAA CCTCGACACC AACTCTAGTC
     2041 CGTGCGAAGA TAAACTAATC GGAGTCGCCC CTCAAATCTT ACAGCTGCTC ACTCCCCTGC
     2101 AGGGCAACGC CCAGGGACCA AGTTAGCCCC TTAAGCCTAG GCAAAAGAAT CCCGCCCATA
     2161 ATCGAGAAGC GACTCGACAT GGAGGCGATG ACGCCCTGAA GTTCCTATTC tctagaaaGt
     2221 ATAGGAACTT Cctgaactgg ccgataattg cagacgATCG AATTCGAGTT GGCGCGCCTG
     2281 TCATTCTAAA TCTCTCTTTC AGCCTAAAGC TTTTTCCCCG TATCCCCCCA GGTGTCTGCA
     2341 GGCTCAAAGA GCAGCGAGAA GCGTTCAGAG GAAAGCGATC CCGTGCCACC TTCCCCGTGC
     2401 CCGGGCTGTC CCCGCACGCT GCCGGCTCGG GGATGCGGGG GGAGCGCCGG ACCGGAGCGG
     2461 AGCCCCGGGC GGCTCGCTGC TGCCCCCTAG CGGGGGAGGG ACGTAATTAC ATCCCTGGGG
     2521 GCTTTGGGAG GGGGCTGTCC CCGTGAGCTC CCAGGCGCGC CTGTCATTCT AAATCTCTCT
     2581 TTCAGCCTAA AGCTTTTTCC CCGTATCCCC CCAGGTGTCT GCAGGCTCAA AGAGCAGCGA
     2641 GAAGCGTTCA GAGGAAAGCG ATCCCGTGCC ACCTTCCCCG TGCCCGGGCT GTCCCCGCAC
     2701 GCTGCCGGCT CGGGGATGCG GGGGGAGCGC CGGACCGGAG CGGAGCCCCG GGCGGCTCGC
     2761 TGCTGCCCCC TAGCGGGGGA GGGACGTAAT TACATCCCTG GGGGCTTTGG GGGGGGGCTG
     2821 TCCCCGTGAG CTCAATAAAA GAGCCCACat aacttcgtat aATGTATGCt atacgaagtt
     2881 ataacgCCAT ATATGGAGTT CCGCGTTACA TAACTTACGG TAAATGGCCC GCCTGGCTGA
     2941 CCGCCCAACG ACCCCCGCCC ATTGACGTCA ATAATGACGT ATGTTCCCAT AGTAACGCCA
     3001 ATAGGGACTT TCCATTGACG TCAATGGGTG GAGTATTTAC GGTAAACTGC CCACTTGGCA
     3061 GTACATCAAG TGTATCATAT GCCAAGTACG CCCCCTATTG ACGTCAATGA CGGTAAATGG
     3121 CCCGCCTGGC ATTATGCCCA GTACATGACC TTATGGGACT TTCCTACTTG GCAGTACATC
     3181 TACGTATTAG TCATCGCTAT TACCATGGTC GAGGTGAGCC CCACGTTCTG CTTCACTCTC
     3241 CCCATCTCCC CCCCCTCCCC ACCCCCAATT TTGTATTTAT TTATTTTTTA ATTATTTTGT
     3301 GCAGCGATGG GGGCGGGGGG GGGGGGGGCG CGCGCCAGGC GGGGCGGGGC GGGGCGAGGG
     3361 GCGGGGCGGG GCGAGGCGGA GAGGTGCGGC GGCAGCCAAT CAGAGCGGCG CGCTCCGAAA
     3421 GTTTCCTTTT ATGGCGAGGC GGCGGCGGCG GCGGCCCTAT AAAAAGCGAA GCGCGCGGCG
     3481 GGCGGGAGTC GCTGCGTTGC CTTCGCCCCG TGCCCCGCTC CGCGCCGCCT CGCGCCGCCC
     3541 GCCCCGGCTC TGACTGACCG CGTTACTCCC ACAGGTGAGC GGGCGGGACG GCCCTTCTCC
     3601 TCCGGGCTGT AATTAGCGCT TGGTTTAATG ACGGCTCGTT TCTTTTCTGT GGCTGCGTGA
     3661 AAGCCTTAAA GGGCTCCGGG AGGGCCCTTT GTGCGGGGGG GAGCGGCTCG GGGGGTGCGT
     3721 GCGTGTGTGT GTGCGTGGGG AGCGCCGCGT GCGGCCCGCG CTGCCCGGCG GCTGTGAGCG
     3781 CTGCGGGCGC GGCGCGGGGC TTTGTGCGCT CCGCGTGTGC GCGAGGGGAG CGCGGCCGGG
     3841 GGCGGTGCCC CGCGGTGCGG GGGGGCTGCG AGGGGAACAA AGGCTGCGTG CGGGGTGTGT
     3901 GCGTGGGGGG GTGAGCAGGG GGTGTGGGCG CGGCGGTCGG GCTGTAACCC CCCCCTGCAC
     3961 CCCCCTCCCC GAGTTGCTGA GCACGGCCCG GCTTCGGGTG CGGGGCTCCG TaCGGGGCGT
     4021 GGCGCGGGGC TCGCCGTGCC GGGCGGGGGG TGGCGGCAGG TGGGGGTGCC GGGCGGGGCG
     4081 GGGCCGCCTC GGGCCGGGGA GGGCTCGGGG GAGGGGCGCG GCGGcCCCCG GAGtGCCGGC
     4141 GGCTGTCGAG GCGCGGCGAG CCGCAGCCAT TGCCTTTTAT GGTAATCGTG CGAGAGGGCG
     4201 CAGGGACTTC CTTTGTCCCA AATCTGTGCG GAGCCGAAAT CTGGGAGGCG CCGCCGCACC
     4261 CCCTCTAGCG GGCGCgGGGC GAAGCGGTGC GGCGCCGGCA GGAAGGAAAT GGGCGGGGAG
     4321 GGCCTTCGTG CGTCGCCGCG CCGCCGTCCC CTTCTCCcTC TCCAGCCTCG GGGCTGTCCG
     4381 CgGGGGGACG GCTGCCTTCG GGGGGGACGG GGCAGGGCGG GGTTCGGCTT CTGGCGTGTG
     4441 ACCGGCGGCT CTAGAGCCTC TGCTAACCAT GTTCATGCCT TCTTCTTTTT CCTACAGCTC
     4501 CTGGGCAACG TGCTGGTTAT TGTGCTGTCT CATCATTTTG GCAAAGaatt cgccacTATG
     4561 gccccAACTG gggtaACCTT TGAGTtctct CAGTTggggg GTTCTggtaa aaagcctgaa
     4621 ctcaccgcga cgtctgtcga gaagtttctg atcgaaaagt tcgacagcgt gtccgacctg
     4681 atgcagctct cggagggcga agaatctcgt gctttcagct tcgatgtagg agggcgtgga
     4741 tatgtcctgc gggtaaatag ctgcgccgat ggtttctaca aagatcgtta tgtttatcgg
     4801 cactttgcat cggccgcgct cccgattccg gaagtgcttg acattgggga atttagcgag
     4861 agcctgacct attgcatctc ccgccgtgca cagggtgtca cgttgcaaga cctgcctgaa
     4921 accgaactgc ccgctgttct gcaaccggtc gcggaggcca tggatgcgat cgctgcggcc
     4981 gatcttagcc agacgagcgg gttcggccca ttcggaccgc aaggaatcgg tcaatacact
     5041 acatggcgtg atttcatatg cgcgattgct gatccccatg tgtatcactg gcaaactgtg
     5101 atggacgaca ccgtcagtgc gtccgtcgcg caggctctcg atgagctgat gctttgggcc
     5161 gaggactgcc ccgaagtccg gcacctcgtg cacgcggatt tcggctccaa caatgtcctg
     5221 acggacaatg gccgcataac agcggtcatt gactggagcg aggcgatgtt cggggattcc
     5281 caatacgagg tcgccaacat cttcttctgg aggccgtggt tggcttgtat ggagcagcag
     5341 acgcgctact tcgagcggag gcatccggag cttgcaggat cgccgcggct ccgggcgtat
     5401 atgctccgca ttggtcttga ccaactctat cagagcttgg ttgacggcaa tttcgatgat
     5461 gcagcttggg cgcagggtcg atgcgacgca atcgtccgat ccggagccgg gactgtcggg
     5521 cgtacacaaa tcgcccgcag aagcgcggcc gtctggaccg atggctgtgt agaagtactc
     5581 gccgatagtg gaaaccgacg ccccagcact cgtccgaggg caaaggaaGG ATCCGGAAGC
     5641 GGAGAGGGCA GAGGAAGTCT GCTAACATGC GGTGACGTCG AGGAGAATCC TGGACCTGGA
     5701 TCCCCCAAGA AGAAGAGGAA GGTGGAGGAC GCCGGATCCg tgtccaaagg agaggagtta
     5761 atcaaggaaa acatgagaat gaaagttgtc atggagggct ccgttaatgg tcaccaattc
     5821 aagtgtacag gggaaggtga aggtaatcct tacatgggta cacaaactat gagaattaaa
     5881 gtaattgaag gcggaccact accatttgca tttgacattc tggcaacgtc attcatgtac
     5941 ggatcacgaa ctttcatcaa gtaccctaaa ggtataccag actttttcaa gcaatctttt
     6001 ccagagggtt ttacatggga aagggttaca agatacgaag atgggggtgt cgtcacagtt
     6061 atgcaagata cttcattaga agatggctgc cttgtctatc atgtgcaagt aagaggggtg
     6121 aattttcctt ctaacggacc tgtgatgcag aaaaagacca aaggttggga accaaatact
     6181 gaaatgatgt acccagctga tggaggtttg agaggctaca cacacatggc gcttaaagtt
     6241 gatggtggag gtcatttgtc ttgtagtttt gttaccactt atcgttctaa aaagactgtt
     6301 ggcaatatca aaatgccagg aatacatgct gtagaccaca gactagaaag actcgaagag
     6361 agcgataacg aaatgttcgt tgtacagaga gagcatgccg tagccaaatt tgctggctta
     6421 ggcggtggta tggatgaatt gtataaggga TCCCATGGCT TCCCGCCGGA GGTGGAGGAG
     6481 CAGGATGATG GCGCGCTGCC CATGTCTTGT GCCCAGGAGA GCGGGATGGA CCGTCACCCT
     6541 GCAGCCTGTG CTTCTGCTAG GATCAATGTG TAACTCGAGT GGCgatccag acatgataag
     6601 atacattgat gagtttggac aaaccacaac tagaatgcag tgaaaaaaat gctttatttg
     6661 tgaaatttgt gatgctattg ctttatttgt aaccattata agctgcaata aacGCTGagc
     6721 agttacagag atgttacgaa ccactagtgc actgcagATC GAATTCGAGT TGGCGCGCCT
     6781 GTCATTCTAA ATCTCTCTTT CAGCCTAAAG CTTTTTCCCC GTATCCCCCC AGGTGTCTGC
     6841 AGGCTCAAAG AGCAGCGAGA AGCGTTCAGA GGAAAGCGAT CCCGTGCCAC CTTCCCCGTG
     6901 CCCGGGCTGT CCCCGCACGC TGCCGGCTCG GGGATGCGGG GGGAGCGCCG GACCGGAGCG
     6961 GAGCCCCGGG CGGCTCGCTG CTGCCCCCTA GCGGGGGAGG GACGTAATTA CATCCCTGGG
     7021 GGCTTTGGGA GGGGGCTGTC CCCGTGAGCT CCCAGGCGCG CCTGTCATTC TAAATCTCTC
     7081 TTTCAGCCTA AAGCTTTTTC CCCGTATCCC CCCAGGTGTC TGCAGGCTCA AAGAGCAGCG
     7141 AGAAGCGTTC AGAGGAAAGC GATCCCGTGC CACCTTCCCC GTGCCCGGGC TGTCCCCGCA
     7201 CGCTGCCGGC TCGGGGATGC GGGGGGAGCG CCGGACCGGA GCGGAGCCCC GGGCGGCTCG
     7261 CTGCTGCCCC CTAGCGGGGG AGGGACGTAA TTACATCCCT GGGGGCTTTG GGGGGGGGCT
     7321 GTCCCCGTGA GCTCAATAAA AGAGCCCACt acaGAAGTTC CTATTCtcta gaaaGtATAG
     7381 GAACTTCGAG Tggaggcagc agagaactcc cataaaggta ttgcggcact cccctccccc
     7441 tgcccagaag ggtgcggcct tctctccacc tcctccaccg cagctccctc aggattgcag
     7501 ctcgcgccgg tttttggaga acaagcgcct cccacccaca aaccagccgg accgaccccc
     7561 gctcctcccc cacccccacg agtgcctgta gcaggtcggg cttgtctcgc ccttcaggcg
     7621 gtgggaaccc Agggcggagc cTcggccgcc gccatccaga agtctcggcc ggcagcccgA
     7681 ccccTcctcc agcgcgcgct tcAtgccacg ttgcgcaggg gcgcggggAc agacactgcg
     7741 gcgctcggcG tcggggagga ccgtaccaac gcccgcctcc ccTccacccc cgcgccccgc
     7801 gcagtggttt cgctcatgtg agactcgaGT AAAACGACGG CCAGTCCGAg cggccgcgtg
     7861 ttacaaccaa ttaaccaatt ctgat
//