LOCUS       MTK234_057              2092 bp ds-DNA     circular     22-FEB-2019
DEFINITION  .
ACCESSION   <unknown id>                                                       
VERSION     <unknown id>                                                       
KEYWORDS    "sgRNA Dropout"                                                    
FEATURES             Location/Qualifiers
     promoter             complement(1945..2049)
                          /label="CamR Promoter"
                          /ApEinfo_revcolor=#84b0dc
                          /ApEinfo_fwdcolor=#84b0dc
     terminator           complement(1176..1284)
                          /label="CamR Terminator"
                          /ApEinfo_revcolor=#84b0dc
                          /ApEinfo_fwdcolor=#84b0dc
     misc_feature         1071..1081
                          /label="modified stem loop"
                          /ApEinfo_revcolor=#85dae9
                          /ApEinfo_fwdcolor=#85dae9
     misc_recomb          931..964
                          /label="LoxP recombination site"
                          /ApEinfo_revcolor=#c7b0e3
                          /ApEinfo_fwdcolor=#c7b0e3
     misc_feature         1083..1094
                          /label="modified stem loop"
                          /ApEinfo_revcolor=#85dae9
                          /ApEinfo_fwdcolor=#85dae9
     misc_feature         1096..1162
                          /label="modified stem loop"
                          /ApEinfo_revcolor=#85dae9
                          /ApEinfo_fwdcolor=#85dae9
     rep_origin           complement(join(2050..2092,1..721))
                          /label="ColE1"
                          /ApEinfo_revcolor=#7f7f7f
                          /ApEinfo_fwdcolor=#7f7f7f
     protein_bind         complement(1168..1173)
                          /label="BsaI"
                          /ApEinfo_revcolor=#b1ff67
                          /ApEinfo_fwdcolor=#b1ff67
     CDS                  complement(1285..1944)
                          /label="CamR"
                          /ApEinfo_revcolor=#0000ff
                          /ApEinfo_fwdcolor=#0000ff
     sgSafeTargetingChr20 1052..1069
                          /label="sgSafeTargetingChr20"
                          /ApEinfo_revcolor=#f8d3a9
                          /ApEinfo_fwdcolor=#f8d3a9
     protein_bind         complement(1163..1166)
                          /label="BsaI"
                          /ApEinfo_revcolor=#b1ff67
                          /ApEinfo_fwdcolor=#b1ff67
     protein_bind         726..731
                          /label="BsaI"
                          /ApEinfo_revcolor=#b1ff67
                          /ApEinfo_fwdcolor=#b1ff67
     protein_bind         733..736
                          /label="BsaI"
                          /ApEinfo_revcolor=#b1ff67
                          /ApEinfo_fwdcolor=#b1ff67
     misc_feature         737..1045
                          /label="mU6 promoter (modified)"
                          /ApEinfo_revcolor=#ffef86
                          /ApEinfo_fwdcolor=#ffef86
ORIGIN
        1 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
       61 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
      121 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
      181 tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
      241 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
      301 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
      361 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
      421 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
      481 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
      541 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
      601 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt
      661 ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg
      721 tagtcggtct cAaacggatc cgacgcgcca tctctaggcc cgcgccggcc ccctcgcacg
      781 gacttgtggg agaagctcgg ctactcccct gccccggtta atttgcatat aatatttcct
      841 agtaactata gaggcttaat gtgcgataaa agacagataa tctgttcttt ttaatactag
      901 ctacatttta catgataggc ttggatttct ataacttcgt atagcataca ttatacgaag
      961 ttataaacag cacaaaagga aactcaccct aactgtaaag taattgtgtg ttttgagact
     1021 ataaGtatcc cttggagaaC CAcctTGTTT GGGCAAGCAC AATGTACTCG TTTAAGAGCT
     1081 AAGCTGGAAA CAGCATAGCA AGTTTAAATA AGGCTAGTCC GTTATCAACT TGAAAAAGTG
     1141 GCACCGAGTC GGTGCTTTTT TTgctgtgag accagaccaa taaaaaacgc ccggcggcaa
     1201 ccgagcgttc tgaacaaatc cagatggagt tctgaggtca ttactggatc tatcaacagg
     1261 agtccaagcg agctcgatat caaattacgc cccgccctgc cactcatcgc agtactgttg
     1321 taattcatta agcattctgc cgacatggaa gccatcacaa acggcatgat gaacctgaat
     1381 cgccagcggc atcagcacct tgtcgccttg cgtataatat ttgcccatgg tgaaaacggg
     1441 ggcgaagaag ttgtccatat tggccacgtt taaatcaaaa ctggtgaaac tcacccaggg
     1501 attggctgaa acgaaaaaca tattctcaat aaacccttta gggaaatagg ccaggttttc
     1561 accgtaacac gccacatctt gcgaatatat gtgtagaaac tgccggaaat cgtcgtggta
     1621 ttcactccag agcgatgaaa acgtttcagt ttgctcatgg aaaacggtgt aacaagggtg
     1681 aacactatcc catatcacca gctcaccgtc tttcattgcc atacgaaatt ccggatgagc
     1741 attcatcagg cgggcaagaa tgtgaataaa ggccggataa aacttgtgct tatttttctt
     1801 tacggtcttt aaaaaggccg taatatccag ctgaacggtc tggttatagg tacattgagc
     1861 aactgactga aatgcctcaa aatgttcttt acgatgccat tgggatatat caacggtggt
     1921 atatccagtg atttttttct ccattttagc ttccttagct cctgaaaatc tcgataactc
     1981 aaaaaatacg cccggtagtg atcttatttc attatggtga aagttggaac ctcttacgtg
     2041 cccgatcaat catgaccaaa atcccttaac gtgagttttc gttccactga gc
//