LOCUS       Exported                2094 bp ds-DNA     circular SYN 22-FEB-2019
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2094)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Jul 30, 2019 from SnapGene 4.3.8.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..2094
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     rep_origin      complement(1..613)
                     /direction=LEFT
                     /label=ColE1
                     /note="color: #7f7f7f"
     protein_bind    618..623
                     /label=BsaI
                     /note="color: #b1ff67"
     protein_bind    625..628
                     /label=BsaI
                     /note="color: #b1ff67"
     misc_feature    629..943
                     /label=mU6 promoter (modified)
                     /note="color: #ffef86"
     misc_recomb     823..856
                     /label=LoxP recombination site
                     /note="color: #c7b0e3"
     misc_feature    964..975
                     /label=modified stem loop
                     /note="color: #85dae9"
     misc_feature    977..988
                     /label=modified stem loop
                     /note="color: #85dae9"
     misc_feature    990..1056
                     /label=modified stem loop
                     /note="color: #85dae9"
     protein_bind    complement(1057..1060)
                     /label=BsaI
                     /note="color: #b1ff67; direction: LEFT"
     protein_bind    complement(1062..1067)
                     /label=BsaI
                     /note="color: #b1ff67; direction: LEFT"
     terminator      complement(1070..1178)
                     /label=CamR Terminator
                     /note="color: #84b0dc; direction: LEFT"
     CDS             complement(1179..1838)
                     /label=CamR
                     /note="color: #0000ff; direction: LEFT"
     promoter        complement(1839..1943)
                     /label=CamR Promoter
                     /note="color: #84b0dc; direction: LEFT"
     rep_origin      complement(1944..2094)
                     /direction=LEFT
                     /label=ColE1
                     /note="color: #7f7f7f"
ORIGIN
        1 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
       61 accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
      121 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
      181 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
      241 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
      301 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
      361 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
      421 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
      481 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
      541 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc
      601 tgtggataac cgtagtcggt ctccaacgga tccgacgcgc catctctagg cccgcgccgg
      661 ccccctcgca cggacttgtg ggagaagctc ggctactccc ctgccccggt taatttgcat
      721 ataatatttc ctagtaacta tagaggctta atgtgcgata aaagacagat aatctgttct
      781 ttttaatact agctacattt tacatgatag gcttggattt ctataacttc gtatagcata
      841 cattatacga agttataaac agcacaaaag gaaactcacc ctaactgtaa agtaattgtg
      901 tgttttgaga ctataagtat cccttggaga accaccttgt ttgcgacacc aactctagtc
      961 cgtgtttaag agctaagctg gaaacagcat agcaagttta aataaggcta gtccgttatc
     1021 aacttgaaaa agtggcaccg agtcggtgct ttttttgctg tgagaccaga ccaataaaaa
     1081 acgcccggcg gcaaccgagc gttctgaaca aatccagatg gagttctgag gtcattactg
     1141 gatctatcaa caggagtcca agcgagctcg atatcaaatt acgccccgcc ctgccactca
     1201 tcgcagtact gttgtaattc attaagcatt ctgccgacat ggaagccatc acaaacggca
     1261 tgatgaacct gaatcgccag cggcatcagc accttgtcgc cttgcgtata atatttgccc
     1321 atggtgaaaa cgggggcgaa gaagttgtcc atattggcca cgtttaaatc aaaactggtg
     1381 aaactcaccc agggattggc tgaaacgaaa aacatattct caataaaccc tttagggaaa
     1441 taggccaggt tttcaccgta acacgccaca tcttgcgaat atatgtgtag aaactgccgg
     1501 aaatcgtcgt ggtattcact ccagagcgat gaaaacgttt cagtttgctc atggaaaacg
     1561 gtgtaacaag ggtgaacact atcccatatc accagctcac cgtctttcat tgccatacga
     1621 aattccggat gagcattcat caggcgggca agaatgtgaa taaaggccgg ataaaacttg
     1681 tgcttatttt tctttacggt ctttaaaaag gccgtaatat ccagctgaac ggtctggtta
     1741 taggtacatt gagcaactga ctgaaatgcc tcaaaatgtt ctttacgatg ccattgggat
     1801 atatcaacgg tggtatatcc agtgattttt ttctccattt tagcttcctt agctcctgaa
     1861 aatctcgata actcaaaaaa tacgcccggt agtgatctta tttcattatg gtgaaagttg
     1921 gaacctctta cgtgcccgat caatcatgac caaaatccct taacgtgagt tttcgttcca
     1981 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     2041 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gttt
//
