LOCUS       ARB346                  4334 bp ds-DNA     circular     22-FEB-2019
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature         2833..2833
                          /label="C in MTK2-2"
                          /ApEinfo_revcolor=#ff9ccd
                          /ApEinfo_fwdcolor=#ff9ccd
     misc_feature         2323..2729
                          /label="CMV Enhancer"
                          /ApEinfo_revcolor=#84b0dc
                          /ApEinfo_fwdcolor=#84b0dc
     protein_bind         complement(4162..4165)
                          /label="BsmBI"
                          /ApEinfo_revcolor=#b1ff67
                          /ApEinfo_fwdcolor=#b1ff67
     rep_origin           complement(1095..1858)
                          /label="ColE1"
                          /ApEinfo_revcolor=#7f7f7f
                          /ApEinfo_fwdcolor=#7f7f7f
     CDS                  complement(106..963)
                          /label="AmpR"
                          /ApEinfo_revcolor=#9f4240
                          /ApEinfo_fwdcolor=#9f4240
     misc_feature         1886..2028
                          /label="Con1"
                          /ApEinfo_revcolor=#ffef86
                          /ApEinfo_fwdcolor=#ffef86
     misc_feature         2854..2875
                          /label="kozac+MCS"
                          /ApEinfo_revcolor=#d59687
                          /ApEinfo_fwdcolor=#d59687
     misc_feature         2110..2123
                          /label="CTCF"
                          /ApEinfo_revcolor=#faac61
                          /ApEinfo_fwdcolor=#faac61
     misc_feature         4173..4315
                          /label="Con2"
                          /ApEinfo_revcolor=#ffef86
                          /ApEinfo_fwdcolor=#ffef86
     misc_feature         2327..2327
                          /label="G in MTK2-2"
                          /ApEinfo_revcolor=#d6b295
                          /ApEinfo_fwdcolor=#d6b295
     promoter             complement(964..1094)
                          /label="AmpR Promoter"
                          /ApEinfo_revcolor=#f58a5e
                          /ApEinfo_fwdcolor=#f58a5e
     misc_feature         complement(3088..3107)
                          /label="sgrna"
                          /ApEinfo_revcolor=#ffef86
                          /ApEinfo_fwdcolor=#ffef86
     misc_feature         2265..2853
                          /label="CMV-unmod"
                          /ApEinfo_revcolor=#c7b0e3
                          /ApEinfo_fwdcolor=#c7b0e3
     sig_peptide          2880..2906
                          /label="NES_(zhang)"
                          /ApEinfo_revcolor=#ff0f00
                          /ApEinfo_fwdcolor=#ff0f00
     Mitochondrial marker 2913..3584
                          /label="MTS-AzamiGreen"
                          /ApEinfo_revcolor=#b1ff67
                          /ApEinfo_fwdcolor=#b1ff67
     misc_feature         2036..2060
                          /label="Con1 scar"
                          /ApEinfo_revcolor=#ffef86
                          /ApEinfo_fwdcolor=#ffef86
     terminator           3601..4116
                          /label="rb_glob_PA_terminator"
                          /ApEinfo_revcolor=#b1ff67
                          /ApEinfo_fwdcolor=#b1ff67
     protein_bind         2029..2034
                          /label="BsmBI"
                          /ApEinfo_revcolor=#b1ff67
                          /ApEinfo_fwdcolor=#b1ff67
     protein_bind         complement(4167..4172)
                          /label="BsmBI"
                          /ApEinfo_revcolor=#b1ff67
                          /ApEinfo_fwdcolor=#b1ff67
     misc_feature         2818..2824
                          /label="TATA BOX"
                          /ApEinfo_revcolor=#9eafd2
                          /ApEinfo_fwdcolor=#9eafd2
     protein_bind         2036..2039
                          /label="BsmBI"
                          /ApEinfo_revcolor=#b1ff67
                          /ApEinfo_fwdcolor=#b1ff67
     misc_feature         2061..2260
                          /label="B1 insulator"
                          /ApEinfo_revcolor=#c7b0e3
                          /ApEinfo_fwdcolor=#c7b0e3
     terminator           complement(9..105)
                          /label="AmpR Terminator"
                          /ApEinfo_revcolor=#f58a5e
                          /ApEinfo_fwdcolor=#f58a5e
ORIGIN
        1 gcggccgcga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
       61 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
      121 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
      181 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
      241 gcgggaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
      301 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
      361 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
      421 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
      481 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
      541 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
      601 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
      661 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
      721 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
      781 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
      841 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
      901 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
      961 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
     1021 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
     1081 aaaagtgcca cctgtcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
     1141 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     1201 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     1261 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct
     1321 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     1381 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     1441 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     1501 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     1561 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
     1621 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     1681 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     1741 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
     1801 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtgc
     1861 ggccgcccct gaattcgcat ctagaacggg gtcatcacgg ctcatcatgc gccaaacaaa
     1921 tgtgtgcaat acacgctcgg atgactgcat gatgaccgca ctgactgggg acagcagatc
     1981 cacctaagcc tgtgagagaa gcagacaccc gacagatcaa ggcagttacg tctcaccaaa
     2041 ccagatgtca acacagctac ggtctgccgc ttcaggtcca tttacggaag ccgcaatcct
     2101 gcactatgac caccaggggg agcgctaggc ccatccctgc acaggtagct atttttagag
     2161 gttgcttcta gcctggagga agaaacgaaa gaaataaaag actatgatac tttttttttt
     2221 taaccggacc aacttataaa ttatttgtaa gtccaataaa aacgtagtta ttaatagtaa
     2281 tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg
     2341 gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg
     2401 tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta
     2461 cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtac gccccctatt
     2521 gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac cttatgggac
     2581 tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt
     2641 tggcagtaca tcaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac
     2701 cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt
     2761 cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat
     2821 ataagcagag ctggtttagt gaaccgtcag atcCGCTAGC GCTACCGGTC GCCACTATGC
     2881 TGCCCCCCCT GGAGAGGCTG ACCCTGGGTT CTgtgagcgt gatcaagccc gagatgaaga
     2941 tcaagctgtg catgaggggc accgtgaacg gccacaactt cgtgatcgag ggcgagggca
     3001 agggcaaccc ctacgagggc acccagatcc tggacctgaa cgtgaccgag ggcgcccccc
     3061 tgcccttcgc ctacgacatc ctgaccaccg tgttccagta cggcaacagg gccttcacca
     3121 agtaccccgc cgacatccag gactacttca agcagacctt ccccgagggc taccactggg
     3181 agaggagcat gacctacgag gaccagggca tctgcaccgc caccagcaac atcagcatga
     3241 ggggcgactg cttcttctac gacatcaggt tcgacggcac caacttcccc cccaacggcc
     3301 ccgtgatgca gaagaagacc ctgaagtggg agcccagcac cgagaagatg tacgtggagg
     3361 acggcgtgct gaagggcgac gtgaacatga ggctgctgct ggagggcggc ggccactaca
     3421 ggtgcgactt caagaccacc tacaaggcca agaaggaggt gaggctgccc gacgcccaca
     3481 agatcgacca caggatcgag atcctgaagc acgacaagga ctacaacaag gtgaagctgt
     3541 acgagaacgc cgtggccagg tactccatgc tgcccagcca ggccggatcc TAACTCGAGA
     3601 TTCACTCCTC AGGTGCAGGC TGCCTATCAG AAGGTGGTGG CTGGTGTGGC CAATGCCCTG
     3661 GCTCACAAAT ACCACTGAGA TCTTTTTCCC TCTGCCAAAA ATTATGGGGA CATCATGAAG
     3721 CCCCTTGAGC ATCTGACTTC TGGCTAATAA AGGAAATTTA TTTTCATTGC AATAGTGTGT
     3781 TGGAATTTTT TGTGTCTCTC ACTCGGAAGG ACATATGGGA GGGCAAATCA TTTAAAACAT
     3841 CAGAATGAGT ATTTGGTTTA GAGTTTGGCA ACATATGcCC ATATGCTGGC TGCCATGAAC
     3901 AAAGGtTGGC TATAAAGAGG TCATCAGTAT ATGAAACAGC CCCCTGCTGT CCATTCCTTA
     3961 TTCCATAGAA AAGCCTTGAC TTGAGGTTAG ATTTTTTTTA TATTTTGTTT TGTGTTATTT
     4021 TTTTCTTTAA CATCCCTAAA ATTTTCCTTA CATGTTTTAC TAGCCAGATT TTTCCTCCTC
     4081 TCCTGACTAC TCCCAGTCAT AGCTGTCCCT CTTCTCTTAT GgAGATCCCT CGACCTGCAG
     4141 CCCAAGCTTG GCGTAATGCT Ggatgtgaga cgataaatcg cagccaagtg agtgaataga
     4201 tgacgcacca cggtcagaca cggccacatc gtatctcaca ggagcaagcg cgataggagc
     4261 actcacacat agtacggtga tccgctgact cctttgccca aataagacgt gagccactag
     4321 tgcactgcag taca
//