LOCUS       ARB351                  4997 bp ds-DNA     circular     22-FEB-2019
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    1886..2028
                     /label="Con2"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    2040..2060
                     /label="Con2 scar"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     CDS             complement(106..963)
                     /label="AmpR"
                     /ApEinfo_revcolor=#9f4240
                     /ApEinfo_fwdcolor=#9f4240
     misc_feature    2824..2843
                     /label="sgRNA"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     misc_feature    2112..2125
                     /label="ctcf"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    4836..4978
                     /label="ConE"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     Terminator      4691..4820
                     /label="SV40-PA"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     regulatory      2273..2560
                     /label="CAG_enhancer"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     protein_bind    complement(4830..4835)
                     /label="BsmBI"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     protein_bind    2036..2039
                     /label="BsmBI"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    2061..2247
                     /label="C3 Insulator"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     Membrane marker 3926..3961
                     /label="Lyn"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     protein_bind    2029..2034
                     /label="BsmBI"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     protein_bind    complement(4825..4828)
                     /label="BsmBI"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     rep_origin      complement(1095..1858)
                     /label="ColE1"
                     /ApEinfo_revcolor=#7f7f7f
                     /ApEinfo_fwdcolor=#7f7f7f
     primer          3870..3889
                     /label="pCAG_F_primer"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     CDS             3968..4675
                     /label="mRuby2"
                     /ApEinfo_revcolor=#ff454e
                     /ApEinfo_fwdcolor=#ff454e
     promoter        complement(964..1094)
                     /label="AmpR Promoter"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    2252..3916
                     /label="CAG promotor"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     terminator      complement(9..105)
                     /label="AmpR Terminator"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
ORIGIN
        1 gcggccgcga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
       61 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
      121 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
      181 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
      241 gcgggaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
      301 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
      361 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
      421 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
      481 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
      541 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
      601 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
      661 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
      721 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
      781 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
      841 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
      901 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
      961 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
     1021 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
     1081 aaaagtgcca cctgtcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
     1141 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     1201 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     1261 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct
     1321 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     1381 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     1441 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     1501 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     1561 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
     1621 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     1681 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     1741 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
     1801 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtgc
     1861 ggccgcccct gaattcgcat ctagaataaa tcgcagccaa gtgagtgaat agatgacgca
     1921 ccacggtcag acacggccac atcgtatctc acaggagcaa gcgcgatagg agcactcaca
     1981 catagtacgg tgatccgctg actcctttgc ccaaataaga cgtgagcccg tctcagatgc
     2041 acacactggc ttaagatgac gggaccctgt aaacgtgtcc gccttgccac tttgtgcaat
     2101 atttagctcc ttcagtagag ggcgctgcag gaacatgtca ggagggggcc ttctcttccg
     2161 gattctggat tctggtatac acttggagcg tggcatttgg gggacagcca ctggtgcgca
     2221 gccccatcaa gttttggtgg catccctaac gCCATATATG GAGTTCCGCG TTACATAACT
     2281 TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA CGTCAATAAT
     2341 GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT GGGTGGAGTA
     2401 TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA GTACGCCCCC
     2461 TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA TGACCTTATG
     2521 GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA TGGTCGAGGT
     2581 GAGCCCCACG TTCTGCTTCA CTCTCCCCAT CTCCCCCCCC TCCCCACCCC CAATTTTGTA
     2641 TTTATTTATT TTTTAATTAT TTTGTGCAGC GATGGGGGCG GGGGGGGGGG GGGCGCGCGC
     2701 CAGGCGGGGC GGGGCGGGGC GAGGGGCGGG GCGGGGCGAG GCGGAGAGGT GCGGCGGCAG
     2761 CCAATCAGAG CGGCGCGCTC CGAAAGTTTC CTTTTATGGC GAGGCGGCGG CGGCGGCGGC
     2821 CCTATAAAAA GCGAAGCGCG CGGCGGGCGG GAGTCGCTGC GTTGCCTTCG CCCCGTGCCC
     2881 CGCTCCGCGC CGCCTCGCGC CGCCCGCCCC GGCTCTGACT GACCGCGTTA CTCCCACAGG
     2941 TGAGCGGGCG GGACGGCCCT TCTCCTCCGG GCTGTAATTA GCGCTTGGTT TAATGACGGC
     3001 TCGTTTCTTT TCTGTGGCTG CGTGAAAGCC TTAAAGGGCT CCGGGAGGGC CCTTTGTGCG
     3061 GGGGGGAGCG GCTCGGGGGG TGCGTGCGTG TGTGTGTGCG TGGGGAGCGC CGCGTGCGGC
     3121 CCGCGCTGCC CGGCGGCTGT GAGCGCTGCG GGCGCGGCGC GGGGCTTTGT GCGCTCCGCG
     3181 TGTGCGCGAG GGGAGCGCGG CCGGGGGCGG TGCCCCGCGG TGCGGGGGGG CTGCGAGGGG
     3241 AACAAAGGCT GCGTGCGGGG TGTGTGCGTG GGGGGGTGAG CAGGGGGTGT GGGCGCGGCG
     3301 GTCGGGCTGT AACCCCCCCC TGCACCCCCC TCCCCGAGTT GCTGAGCACG GCCCGGCTTC
     3361 GGGTGCGGGG CTCCGTaCGG GGCGTGGCGC GGGGCTCGCC GTGCCGGGCG GGGGGTGGCG
     3421 GCAGGTGGGG GTGCCGGGCG GGGCGGGGCC GCCTCGGGCC GGGGAGGGCT CGGGGGAGGG
     3481 GCGCGGCGGc CCCCGGAGcG CCGGCGGCTG TCGAGGCGCG GCGAGCCGCA GCCATTGCCT
     3541 TTTATGGTAA TCGTGCGAGA GGGCGCAGGG ACTTCCTTTG TCCCAAATCT GTGCGGAGCC
     3601 GAAATCTGGG AGGCGCCGCC GCACCCCCTC TAGCGGGCGC gGGGCGAAGC GGTGCGGCGC
     3661 CGGCAGGAAG GAAATGGGCG GGGAGGGCCT TCGTGCGTCG CCGCGCCGCC GTCCCCTTCT
     3721 CCcTCTCCAG CCTCGGGGCT GTCCGCgGGG GGACGGCTGC CTTCGGGGGG GACGGGGCAG
     3781 GGCGGGGTTC GGCTTCTGGC GTGTGACCGG CGGCTCTAGA GCCTCTGCTA ACCATGTTCA
     3841 TGCCTTCTTC TTTTTCCTAC AGCTCCTGGG CAACGTGCTG GTTATTGTGC TGTCTCATCA
     3901 TTTTGGCAAA Gaattcgcca cTATGggctg tatcaagagc aagaggaagg acgtcgagaa
     3961 cGGTTCTgtg tccaaaggag aggagttaat caaggaaaac atgagaatga aagttgtcat
     4021 ggagggctcc gttaatggtc accaattcaa gtgtacaggg gaaggtgaag gtaatcctta
     4081 catgggtaca caaactatga gaattaaagt aattgaaggc ggaccactac catttgcatt
     4141 tgacattctg gcaacgtcat tcatgtacgg atcacgaact ttcatcaagt accctaaagg
     4201 tataccagac tttttcaagc aatcttttcc agagggtttt acatgggaaa gggttacaag
     4261 atacgaagat gggggtgtcg tcacagttat gcaagatact tcattagaag atggctgcct
     4321 tgtctatcat gtgcaagtaa gaggggtgaa ttttccttct aacggacctg tgatgcagaa
     4381 aaagaccaaa ggttgggaac caaatactga aatgatgtac ccagctgatg gaggtttgag
     4441 aggctacaca cacatggcgc ttaaagttga tggtggaggt catttgtctt gtagttttgt
     4501 taccacttat cgttctaaaa agactgttgg caatatcaaa atgccaggaa tacatgctgt
     4561 agaccacaga ctagaaagac tcgaagagag cgataacgaa atgttcgttg tacagagaga
     4621 gcatgccgta gccaaatttg ctggcttagg cggtggtatg gatgaattgt ataagggatc
     4681 cTAACTCGAG gatccagaca tgataagata cattgatgag tttggacaaa ccacaactag
     4741 aatgcagtga aaaaaatgct ttatttgtga aatttgtgat gctattgctt tatttgtaac
     4801 cattataagc tgcaataaac GCTGagcatg agacggaaat ctgctcgtca gtggtgctca
     4861 cactgacgaa tcatgtacag atcataccga tgactgcctg gcgactcaca actaagcaag
     4921 acagccggaa ccagcgccgg cgaacaccac tgcatatatg gcatatcaca acagtccaac
     4981 tagtgcactg cagtaca
//