LOCUS       JPF0454a__pHR-PGK-CAS9 13897 bp ds-DNA     circular     07-MAY-2018
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature         784..789
                          /label="Bbs1"
                          /ApEinfo_revcolor=#b1ff67
                          /ApEinfo_fwdcolor=#b1ff67
     misc_feature         complement(13347..13366)
                          /label="sgrna"
                          /ApEinfo_revcolor=#ffef86
                          /ApEinfo_fwdcolor=#ffef86
     misc_feature         5518..5521
                          /label="Bbs1"
                          /ApEinfo_revcolor=#b1ff67
                          /ApEinfo_fwdcolor=#b1ff67
     misc_feature         8283..8298
                          /label="cPPt"
                          /ApEinfo_revcolor=#f58a5e
                          /ApEinfo_fwdcolor=#f58a5e
     misc_feature         111..686
                          /label="WPRE"
                          /ApEinfo_revcolor=#85dae9
                          /ApEinfo_fwdcolor=#85dae9
     misc_feature         6261..6266
                          /label="Bbs1"
                          /ApEinfo_revcolor=#b1ff67
                          /ApEinfo_fwdcolor=#b1ff67
     misc_feature         2601..2748
                          /label="KanR Promoter"
                          /ApEinfo_revcolor=#b1ff67
                          /ApEinfo_fwdcolor=#b1ff67
     misc_feature         743..758
                          /label="cPPT"
                          /ApEinfo_revcolor=#d6b295
                          /ApEinfo_fwdcolor=#d6b295
     misc_feature         2749..3564
                          /label="KanR"
                          /ApEinfo_revcolor=#008000
                          /ApEinfo_fwdcolor=#008000
     misc_feature         complement(638..641)
                          /label="Bbs1"
                          /ApEinfo_revcolor=#b1ff67
                          /ApEinfo_fwdcolor=#b1ff67
     Mitochondrial marker 13172..13843
                          /label="MTS-AzamiGreen"
                          /ApEinfo_revcolor=#b1ff67
                          /ApEinfo_fwdcolor=#b1ff67
     misc_feature         6981..7025
                          /label="psi packaging signal"
                          /ApEinfo_revcolor=#b4abac
                          /ApEinfo_fwdcolor=#b4abac
     misc_feature         813..993
                          /label="HIV LTR truncated (3')"
                          /ApEinfo_revcolor=#b1ff67
                          /ApEinfo_fwdcolor=#b1ff67
     promoter             8481..9003
                          /label="pgk1_promoter"
                          /ApEinfo_revcolor=#0080ff
                          /ApEinfo_fwdcolor=#0080ff
     misc_feature         6269..6272
                          /label="Bbs1"
                          /ApEinfo_revcolor=#b1ff67
                          /ApEinfo_fwdcolor=#b1ff67
     misc_feature         13853..13889
                          /label="Spacer"
                          /ApEinfo_revcolor=#c6c9d1
                          /ApEinfo_fwdcolor=#c6c9d1
     misc_feature         1837..2600
                          /label="ColE1"
                          /ApEinfo_revcolor=#7f7f7f
                          /ApEinfo_fwdcolor=#7f7f7f
     misc_feature         792..795
                          /label="Bbs1"
                          /ApEinfo_revcolor=#b1ff67
                          /ApEinfo_fwdcolor=#b1ff67
     misc_feature         3565..3591
                          /label="KanR Terminator"
                          /ApEinfo_revcolor=#b1ff67
                          /ApEinfo_fwdcolor=#b1ff67
     misc_feature         9008..13120
                          /label="Cas9"
                          /ApEinfo_revcolor=#75c6a9
                          /ApEinfo_fwdcolor=#75c6a9
     misc_feature         9008..13165
                          /label="Cas9-2xNLS"
                          /ApEinfo_revcolor=#f58a5e
                          /ApEinfo_fwdcolor=#f58a5e
     misc_feature         complement(644..649)
                          /label="Bbs1"
                          /ApEinfo_revcolor=#b1ff67
                          /ApEinfo_fwdcolor=#b1ff67
     misc_feature         5510..5515
                          /label="Bbs1"
                          /ApEinfo_revcolor=#b1ff67
                          /ApEinfo_fwdcolor=#b1ff67
     misc_feature         1679..1701
                          /label="pGEX"
                          /ApEinfo_revcolor=#85dae9
                          /ApEinfo_fwdcolor=#85dae9
     misc_feature         9008..13108
                          /label="Cas9"
                          /ApEinfo_revcolor=#84b0dc
                          /ApEinfo_fwdcolor=#84b0dc
     misc_feature         13118..13165
                          /label="2xNLS (DPKKKRKV)"
                          /ApEinfo_revcolor=#b1ff67
                          /ApEinfo_fwdcolor=#b1ff67
     misc_feature         3565..3591
                          /label="KanR Terminator"
                          /ApEinfo_revcolor=#b1ff67
                          /ApEinfo_fwdcolor=#b1ff67
     misc_feature         6690..6870
                          /label="HIV LTR (truncated 5')"
                          /ApEinfo_revcolor=#75c6a9
                          /ApEinfo_fwdcolor=#75c6a9
ORIGIN
        1 gttacagaga tgttacgaac cactagtgca ctgcagtaca tgactgactg aggccgcgac
       61 tctagagtcg acctgcaggc atgcaagctt gatatcaagc ttatcgataa tcaacctctg
      121 gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta
      181 tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt
      241 ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc
      301 aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt
      361 gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg
      421 gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac
      481 aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc
      541 acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac
      601 cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct
      661 cagacgagtc ggatctccct ttgggccgcc tccccgcatc gataccgtcg accaaggcag
      721 ctgtagatct tagccacttt ttaaaagaaa aggggggact ggaagggcta attcactccc
      781 aacgaagaca agatctgctt tttgcttgta ctgggtttct ctggttagac cagatctgag
      841 cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt
      901 gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca
      961 gaccctttta gtcagtgtgg aaaatctcta gcagcatcta gaattaattc cgtgtattct
     1021 atagtgtcac ctaaatcgta tgtgtatgat acataaggtt atgtattaat tgtagccgcg
     1081 ttctaacgac aatatgtaca agcctaattg tgtagcatct ggcttactga agcagaccct
     1141 atcatctctc tcgtaaactg ccgtcagagt cggtttggtt ggacgaacct tctgagtttc
     1201 tggtaacgcc gtcccgcacc cggaaatggt cagcgaacca atcagcaggg tcatcgctag
     1261 ccagatcctc tacgccggac gcatcgtggc cggcatcacc ggcgccacag gtgcggttgc
     1321 tggcgcctat atcgccgaca tcaccgatgg ggaagatcgg gctcgccact tcgggctcat
     1381 gagcgcttgt ttcggcgtgg gtatggtggc aggccccgtg gccgggggac tgttgggcgc
     1441 catctccttg catgcaccat tccttgcggc ggcggtgctc aacggcctca acctactact
     1501 gggctgcttc ctaatgcagg agtcgcataa gggagagcgt cgaatggtgc actctcagta
     1561 caatctagct ctgatgccgc atagttaagc cagccccgac acccgccaac acccgctgac
     1621 gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccatctcc
     1681 gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag atgaaagggc
     1741 ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca
     1801 ggtggcactt ttcggggaaa tgtgccgagc ggccgcacgg ttatccacag aatcagggga
     1861 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
     1921 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
     1981 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     2041 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
     2101 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
     2161 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
     2221 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     2281 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     2341 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct
     2401 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     2461 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     2521 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
     2581 ttaagggatt ttggtcatga ctgatccttc aactcagcaa aagttcgatt tattcaacaa
     2641 agccacgttg tgtctcaaaa tctctgatgt tacattgcac aagataaaaa tatatcatca
     2701 tgaacaataa aactgtctgc ttacataaac agtaatacaa ggggtgttat gagccatatt
     2761 caacgggaaa cgtcttgctc caggccgcga ttaaattcca acatggatgc tgatttatat
     2821 gggtataaat gggctcgcga taatgtcggg caatcaggtg cgacaatcta tcgattgtat
     2881 gggaagcccg atgcgccaga gttgtttctg aaacatggca aaggtagcgt tgccaatgat
     2941 gttacagatg agatggtcag actaaactgg ctgacggaat ttatgcctct tccgaccatc
     3001 aagcatttta tccgtactcc tgatgatgca tggttactca ccactgcgat ccccgggaaa
     3061 acagcattcc aggtattaga agaatatcct gattcaggtg aaaatattgt tgatgcgctg
     3121 gcagtgttcc tgcgccggtt gcattcgatt cctgtttgta attgtccttt taacagcgat
     3181 cgcgtatttc gcctcgctca ggcgcaatca cgaatgaata acggtttggt tgatgcgagt
     3241 gattttgatg acgagcgtaa tggctggcct gttgaacaag tctggaaaga aatgcataag
     3301 cttttgccat tctcaccgga ttcagtcgtc actcatggtg atttctcact tgataacctt
     3361 atttttgacg aggggaaatt aataggttgt attgatgttg gacgagtcgg aatcgcagac
     3421 cgataccagg atcttgccat cctatggaac tgcctcggtg agttttctcc ttcattacag
     3481 aaacggcttt ttcaaaaata tggtattgat aatcctgata tgaataaatt gcagtttcat
     3541 ttgatgctcg atgagttttt ctaatcagaa ttggttaatt ggttgtaaca cgcggccgcc
     3601 aattttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
     3661 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc
     3721 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc
     3781 aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctgtggaat gtgtgtcagt
     3841 tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca
     3901 attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa
     3961 gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc
     4021 taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg
     4081 cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg
     4141 gaggcctagg cttttgcaaa aagcttggac acaagacagg cttgcgagat atgtttgaga
     4201 ataccacttt atcccgcgtc agggagaggc agtgcgtaaa aagacgcgga ctcatgtgaa
     4261 atactggttt ttagtgcgcc agatctctat aatctcgcgc aacctatttt cccctcgaac
     4321 actttttaag ccgtagataa acaggctggg acacttcaca tgagcgaaaa atacatcgtc
     4381 acctgggaca tgttgcagat ccatgcacgt aaactcgcaa gccgactgat gccttctgaa
     4441 caatggaaag gcattattgc cgtaagccgt ggcggtctgt accgggtgcg ttactggcgc
     4501 gtgaactggg tattcgtcat gtcgataccg tttgtatttc cagctacgat cacgacaacc
     4561 agcgcgagct taaagtgctg aaacgcgcag aaggcgatgg cgaaggcttc atcgttattg
     4621 atgacctggt ggataccggt ggtactgcgg ttgcgattcg tgaaatgtat ccaaaagcgc
     4681 actttgtcac catcttcgca aaaccggctg gtcgtccgct ggttgatgac tatgttgttg
     4741 atatcccgca agatacctgg attgaacagc cgtgggatat gggcgtcgta ttcgtcccgc
     4801 caatctccgg tcgctaatct tttcaacgcc tggcactgcc gggcgttgtt ctttttaact
     4861 tcaggcgggt tacaatagtt tccagtaagt attctggagg ctgcatccat gacacaggca
     4921 aacctgagcg aaaccctgtt caaaccccgc tttaaacatc ctgaaacctc gacgctagtc
     4981 cgccgcttta atcacggcgc acaaccgcct gtgcagtcgg cccttgatgg taaaaccatc
     5041 cctcactggt atcgcatgat taaccgtctg atgtggatct ggcgcggcat tgacccacgc
     5101 gaaatcctcg acgtccaggc acgtattgtg atgagcgatg ccgaacgtac cgacgatgat
     5161 ttatacgata cggtgattgg ctaccgtggc ggcaactgga tttatgagtg ggccccggat
     5221 ctttgtgaag gaaccttact tctgtggtgt gacataattg gacaaactac ctacagagat
     5281 ttaaagctct aaggtaaata taaaattttt aagtgtataa tgtgttaaac tactgattct
     5341 aattgtttgt gtattttaga ttccaaccta tggaactgat gaatgggagc agtggtggaa
     5401 tgcctttaat gaggaaaacc tgttttgctc agaagaaatg ccatctagtg atgatgaggc
     5461 tactgctgac tctcaacatt ctactcctcc aaaaaagaag agaaaggtag aagaccccaa
     5521 ggactttcct tcagaattgc taagtttttt gagtcatgct gtgtttagta atagaactct
     5581 tgcttgcttt gctatttaca ccacaaagga aaaagctgca ctgctataca agaaaattat
     5641 ggaaaaatat tctgtaacct ttataagtag gcataacagt tataatcata acatactgtt
     5701 ttttcttact ccacacaggc atagagtgtc tgctattaat aactatgctc aaaaattgtg
     5761 tacctttagc tttttaattt gtaaaggggt taataaggaa tatttgatgt atagtgcctt
     5821 gactagagat cataatcagc cataccacat ttgtagaggt tttacttgct ttaaaaaacc
     5881 tcccacacct ccccctgaac ctgaaacata aaatgaatgc aattgttgtt gttaacttgt
     5941 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
     6001 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg
     6061 tctggatcaa ctggataact caagctaacc aaaatcatcc caaacttccc accccatacc
     6121 ctattaccac tgccaattac ctagtggttt catttactct aaacctgtga ttcctctgaa
     6181 ttattttcat tttaaagaaa ttgtatttgt taaatatgta ctacaaactt agtagttgga
     6241 agggctaatt cactcccaaa gaagacaaga tatccttgat ctgtggatct accacacaca
     6301 aggctacttc cctgattagc agaactacac accagggcca ggggtcagat atccactgac
     6361 ctttggatgg tgctacaagc tagtaccagt tgagccagat aaggtagaag aggccaataa
     6421 aggagagaac accagcttgt tacaccctgt gagcctgcat gggatggatg acccggagag
     6481 agaagtgtta gagtggaggt ttgacagccg cctagcattt catcacgtgg cccgagagct
     6541 gcatccggag tacttcaaga actgctgata tcgagcttgc tacaagggac tttccgctgg
     6601 ggactttcca gggaggcgtg gcctgggcgg gactggggag tggcgagccc tcagatcctg
     6661 catataagca gctgcttttt gcctgtactg ggactctctg gttagaccag atctgagcct
     6721 gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag
     6781 tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac
     6841 ccttttagtc agtgtggaaa atctctagca gtggcgcccg aacagggact tgaaagcgaa
     6901 agggaaacca gaggagctct ctcgacgcag gactcggctt gctgaagcgc gcacggcaag
     6961 aggcgagggg cggcgactgg tgagtacgcc aaaaattttg actagcggag gctagaagga
     7021 gagagatggg tgcgagagcg tcagtattaa gcgggggaga attagatcgc gatgggaaaa
     7081 aattcggtta aggccagggg gaaagaaaaa atataaatta aaacatatag tatgggcaag
     7141 cagggagcta gaacgattcg cagttaatcc tggcctgtta gaaacatcag aaggctgtag
     7201 acaaatactg ggacagctac aaccatccct tcagacagga tcagaagaac ttagatcatt
     7261 atataataca gtagcaaccc tctattgtgt gcatcaaagg atagagataa aagacaccaa
     7321 ggaagcttta gacaagatag aggaagagca aaacaaaagt aagaccaccg cacagcaagc
     7381 ggccggtgat cttcagacct ggacgatata tatgagggac aattggagaa gtgaattata
     7441 taaatataaa gtagtaaaaa ttgaaccatt aggagtagca cccaccaagg caaagagaag
     7501 agtggtgcag agagaaaaaa gagcagtggg aataggagct ttgttccttg ggttcttggg
     7561 agcagcagga agcactatgg gcgcagcgtc aatgacgctg acggtacagg ccagacaatt
     7621 attgtctggt atagtgcagc agcagaacaa tttgctgagg gctattgagg cgcaacagca
     7681 tctgttgcaa ctcacagtct ggggcatcaa gcagctccag gcaagaatcc tggctgtgga
     7741 aagataccta aaggatcaac agctcctggg gatttggggt tgctctggaa aactcatttg
     7801 caccactgct gtgccttgga atgctagttg gagtaataaa tctctggaac agatttggaa
     7861 tcacacgacc tggatggagt gggacagaga aattaacaat tacacaagct taatacactc
     7921 cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa caagaattat tggaattaga
     7981 taaatgggca agtttgtgga attggtttaa cataacaaat tggctgtggt atataaaatt
     8041 attcataatg atagtaggag gcttggtagg tttaagaata gtttttgctg tactttctat
     8101 agtgaataga gttaggcagg gatattcacc attatcgttt cagacccacc tcccaacccc
     8161 gaggggaccc gacaggcccg aaggaataga agaagaaggt ggagagagag acagagacag
     8221 atccattcga ttagtgaacg gatctcgacg gtcgccaaat ggcagtattc atccacaatt
     8281 ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag
     8341 caacagacat acaaactaaa gaattacaaa aacaaattac aaaaattcaa aattttcggg
     8401 tttattacag ggacagcaga gatccagttt ggccctgaat tcgcatctag actgaactgg
     8461 ccgataattg cagacgaacg tcgaattccc acggggttgg ggttgcgcct tttccaaggc
     8521 agccctgggt ttgcgcaggg acgcggctgc tctgggcgtg gttccgggaa acgcagcggc
     8581 gccgaccctg ggtctTgcac attcttcacg tccgttcgca gcgtcacccg gatcttcgcc
     8641 gctacccttg tgggcccccc ggcgacgctt cctgctccgc ccctaagtcg ggaaggttcc
     8701 ttgcggttcg cggcgtgccg gacgtgacaa acggaagccg cacgtGtcac tagtaccctc
     8761 gcagacggac agcgccaggg agcaatggca gcgcgccgac cgcgatgggc tgtggccaat
     8821 agcggctgct cagcAgggcg cgccgagagc agcggccggg aaggggcggt gcgggaggcg
     8881 gggtgtgggg cggtagtgtg ggccctgttc ctgcccgcgc ggtgttccgc attctgcaag
     8941 cctccggagc gcacgtcggc agtcggctcc ctcgttgacc gaatcaccga cctctctccc
     9001 cagTATGgac aagaagtatt ctatcggact ggatatcggg actaatagcg tcgggtgggc
     9061 cgtgatcact gacgagtaca aggtgccctc taagaagttc aaggtgctcg ggaacaccga
     9121 ccggcattcc atcaagaaaa atctgatcgg agctctcctc tttgattcag gggaaaccgc
     9181 tgaagcaacc cgcctcaagc ggactgctag acggcggtac accaggagga agaaccggat
     9241 ttgttacctt caagagatat tctccaacga aatggcaaag gtcgacgaca gcttcttcca
     9301 taggctggaa gaatcattcc tcgtggaaga ggataagaag catgaacggc atcccatctt
     9361 cggtaatatc gtcgacgagg tggcctatca cgagaaatac ccaaccatct accatcttcg
     9421 caaaaagctg gtggactcaa ccgacaaggc agacctccgg cttatctacc tggccctggc
     9481 ccacatgatc aagttcagag gccacttcct gatcgagggc gacctcaatc ctgacaatag
     9541 cgatgtggat aaactgttca tccagctggt gcagacttac aaccagctct ttgaagagaa
     9601 ccccatcaat gcaagcggag tcgatgccaa ggccattctg tcagcccggc tgtcaaagag
     9661 ccgcagactt gagaatctta tcgctcagct gccgggtgaa aagaaaaatg gactgttcgg
     9721 gaacctgatt gctctttcac ttgggctgac tcccaatttc aagtctaatt tcgacctggc
     9781 agaggatgcc aagctgcaac tgtccaagga cacctatgat gacgatctcg acaacctcct
     9841 ggcccagatc ggtgaccaat acgccgacct tttccttgct gctaagaatc tttctgacgc
     9901 catcctgctg tctgacattc tccgcgtgaa cactgaaatc accaaggccc ctctttcagc
     9961 ttcaatgatt aagcggtatg atgagcacca ccaggacctg accctgctta aggcactcgt
    10021 ccggcagcag cttccggaga agtacaagga aatcttcttt gaccagtcaa agaatggata
    10081 cgccggctac atcgacggag gtgcctccca agaggaattt tataagttta tcaaacctat
    10141 ccttgagaag atggacggca ccgaagagct cctcgtgaaa ctgaatcggg aggatctgct
    10201 gcggaagcag cgcactttcg acaatgggag cattccccac cagatccatc ttggggagct
    10261 tcacgccatc cttcggcgcc aagaggactt ctaccccttt cttaaggaca acagggagaa
    10321 gattgagaaa attctcactt tccgcatccc ctactacgtg ggacccctcg ccagaggaaa
    10381 tagccggttt gcttggatga ccagaaagtc agaagaaact atcactccct ggaacttcga
    10441 agaggtggtg gacaagggag ccagcgctca gtcattcatc gaacggatga ctaacttcga
    10501 taagaacctc cccaatgaga aggtcctgcc gaaacattcc ctgctctacg agtactttac
    10561 cgtgtacaac gagctgacca aggtgaaata tgtcaccgaa gggatgagga agcccgcatt
    10621 cctgtcaggc gaacaaaaga aggcaattgt ggaccttctg ttcaagacca atagaaaggt
    10681 gaccgtgaag cagctgaagg aggactattt caagaaaatt gaatgcttcg actctgtgga
    10741 gattagcggg gtcgaagatc ggttcaacgc aagcctgggt acttaccatg atctgcttaa
    10801 gatcatcaag gacaaggatt ttctggacaa tgaggagaac gaggacatcc ttgaggacat
    10861 tgtcctgact ctcactctgt tcgaggaccg ggaaatgatc gaggagaggc ttaagaccta
    10921 cgcccatctg ttcgacgata aagtgatgaa gcaacttaaa cggagaagat ataccggatg
    10981 gggacgcctt agccgcaaac tcatcaacgg aatccgggac aaacagagcg gaaagaccat
    11041 tcttgatttc cttaagagcg acggattcgc taatcgcaac ttcatgcaac ttatccatga
    11101 tgattccctg acctttaagg aggacatcca gaaggcccaa gtgtctggac aaggtgactc
    11161 actgcacgag catatcgcaa atctggctgg ttcacccgct attaagaagg gtattctcca
    11221 gaccgtgaaa gtcgtggacg agctggtcaa ggtgatgggt cgccataaac cagagaacat
    11281 tgtcatcgag atggccaggg aaaaccagac tacccagaag ggacagaaga acagcaggga
    11341 gcggatgaaa agaattgagg aagggattaa ggagctcggg tcacagatcc ttaaagagca
    11401 cccggtggaa aacacccagc ttcagaatga gaagctctat ctgtactacc ttcaaaatgg
    11461 acgcgatatg tatgtggacc aagagcttga tatcaacagg ctctcagact acgacgtgga
    11521 ccatatcgtc cctcagagct tcctcaaaga cgactcaatt gacaataagg tgctgactcg
    11581 ctcagacaag aaccggggaa agtcagataa cgtgccctca gaggaagtcg tgaaaaagat
    11641 gaagaactat tggcgccagc ttctgaacgc aaagctgatc actcagcgga agttcgacaa
    11701 tctcactaag gctgagaggg gcggactgag cgaactggac aaagcaggat tcattaaacg
    11761 gcaacttgtg gagactcggc agattactaa acatgtcgcc caaatccttg actcacgcat
    11821 gaataccaag tacgacgaaa acgacaaact tatccgcgag gtgaaggtga ttaccctgaa
    11881 gtccaagctg gtcagcgatt tcagaaagga ctttcaattc tacaaagtgc gggagatcaa
    11941 taactatcat catgctcatg acgcatatct gaatgccgtg gtgggaaccg ccctgatcaa
    12001 gaagtaccca aagctggaaa gcgagttcgt gtacggagac tacaaggtct acgacgtgcg
    12061 caagatgatt gccaaatctg agcaggagat cggaaaggcc accgcaaagt acttcttcta
    12121 cagcaacatc atgaatttct tcaagaccga aatcaccctt gcaaacggtg agatccggaa
    12181 gaggccgctc atcgagacta atggggagac tggcgaaatc gtgtgggaca agggcagaga
    12241 tttcgctacc gtgcgcaaag tgctttctat gcctcaagtg aacatcgtga agaaaaccga
    12301 ggtgcaaacc ggaggctttt ctaaggaatc aatcctcccc aagcgcaact ccgacaagct
    12361 cattgcaagg aagaaggatt gggaccctaa gaagtacggc ggattcgatt caccaactgt
    12421 ggcttattct gtcctggtcg tggctaaggt ggaaaaagga aagtctaaga agctcaagag
    12481 cgtgaaggaa ctgctgggta tcaccattat ggagcgcagc tccttcgaga agaacccaat
    12541 tgactttctc gaagccaaag gttacaagga agtcaagaag gaccttatca tcaagctccc
    12601 aaagtatagc ctgttcgaac tggagaatgg gcggaagcgg atgctcgcct ccgctggcga
    12661 acttcagaag ggtaatgagc tggctctccc ctccaagtac gtgaatttcc tctaccttgc
    12721 aagccattac gagaagctga aggggagccc cgaggacaac gagcaaaagc aactgtttgt
    12781 ggagcagcat aagcattatc tggacgagat cattgagcag atttccgagt tttctaaacg
    12841 cgtcattctc gctgatgcca acctcgataa agtccttagc gcatacaata agcacagaga
    12901 caaaccaatt cgggagcagg ctgagaatat catccacctg ttcaccctca ccaatcttgg
    12961 tgcccctgcc gcattcaagt acttcgacac caccatcgac cggaaacgct atacctccac
    13021 caaagaagtg ctggacgcca ccctcatcca ccagagcatc accggacttt acgaaactcg
    13081 gattgacctc tcacagctcg gaggggatga gggagctgat ccaaaaaaga agagaaaggt
    13141 agatccaaaa aagaagagaa aggtaggttc tgtgagcgtg atcaagcccg agatgaagat
    13201 caagctgtgc atgaggggca ccgtgaacgg ccacaacttc gtgatcgagg gcgagggcaa
    13261 gggcaacccc tacgagggca cccagatcct ggacctgaac gtgaccgagg gcgcccccct
    13321 gcccttcgcc tacgacatcc tgaccaccgt gttccagtac ggcaacaggg ccttcaccaa
    13381 gtaccccgcc gacatccagg actacttcaa gcagaccttc cccgagggct accactggga
    13441 gaggagcatg acctacgagg accagggcat ctgcaccgcc accagcaaca tcagcatgag
    13501 gggcgactgc ttcttctacg acatcaggtt cgacggcacc aacttccccc ccaacggccc
    13561 cgtgatgcag aagaagaccc tgaagtggga gcccagcacc gagaagatgt acgtggagga
    13621 cggcgtgctg aagggcgacg tgaacatgag gctgctgctg gagggcggcg gccactacag
    13681 gtgcgacttc aagaccacct acaaggccaa gaaggaggtg aggctgcccg acgcccacaa
    13741 gatcgaccac aggatcgaga tcctgaagca cgacaaggac tacaacaagg tgaagctgta
    13801 cgagaacgcc gtggccaggt actccatgct gcccagccag gccggatccT AAactagaga
    13861 tctatgtgag gatcctaact cgagatcgaG CTGagca
//