LOCUS       MTK3_028                1877 bp ds-DNA     circular     22-FEB-2019
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    1244..1247
                     /label="Part3"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     misc_feature    complement(1472..1580)
                     /label="CamR Terminator"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     CDS             complement(join(1581..1877,1..363))
                     /label="CamR"
                     /ApEinfo_revcolor=#0000ff
                     /ApEinfo_fwdcolor=#0000ff
     misc_feature    1273..1276
                     /label="Conserved"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    1254..1289
                     /label="Hammer Head Ribozyme"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    1311..1390
                     /label="gDNA scaffolding"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_feature    complement(1248..1253)
                     /label="sgRNA"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    1291..1310
                     /label="sgRNA"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    complement(1291..1310)
                     /label="tet3g promoter"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    1254..1260
                     /label="Conserved"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     promoter        complement(364..468)
                     /label="CamR Promoter"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    1391..1458
                     /label="HDV"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     misc_feature    complement(1296..1310)
                     /label="tetO"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     rep_origin      complement(469..1232)
                     /label="ColE1"
                     /ApEinfo_revcolor=#7f7f7f
                     /ApEinfo_fwdcolor=#7f7f7f
ORIGIN
        1 cgaatatatg tgtagaaact gccggaaatc gtcgtggtat tcactccaga gcgatgaaaa
       61 cgtttcagtt tgctcatgga aaacggtgta acaagggtga acactatccc atatcaccag
      121 ctcaccgtct ttcattgcca tacgaaattc cggatgagca ttcatcaggc gggcaagaat
      181 gtgaataaag gccggataaa acttgtgctt atttttcttt acggtcttta aaaaggccgt
      241 aatatccagc tgaacggtct ggttataggt acattgagca actgactgaa atgcctcaaa
      301 atgttcttta cgatgccatt gggatatatc aacggtggta tatccagtga tttttttctc
      361 cattttagct tccttagctc ctgaaaatct cgataactca aaaaatacgc ccggtagtga
      421 tcttatttca ttatggtgaa agttggaacc tcttacgtgc ccgatcaatc atgaccaaaa
      481 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
      541 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
      601 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
      661 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc
      721 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
      781 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
      841 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
      901 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
      961 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     1021 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     1081 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     1141 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
     1201 ctgcgttatc ccctgattct gtggataacc gtagtcggtc tcaTATGaac gtaCTGATGA
     1261 GTCCGTGAGG ACGAAACGAG TAAGCTCGTC tacgttctct atcactgata GTTTTAGAGC
     1321 TAGAAATAGC AAGTTAAAAT AAGGCTAGTC CGTTATCAAC TTGAAAAAGT GGCACCGAGT
     1381 CGGTGCTTTT GGCCGGCATG GTCCCAGCCT CCTCGCTGGC GCCGGCTGGG CAACATGCTT
     1441 CGGCATGGCG AATGGGACAT CCtgagacca gaccaataaa aaacgcccgg cggcaaccga
     1501 gcgttctgaa caaatccaga tggagttctg aggtcattac tggatctatc aacaggagtc
     1561 caagcgagct cgatatcaaa ttacgccccg ccctgccact catcgcagta ctgttgtaat
     1621 tcattaagca ttctgccgac atggaagcca tcacaaacgg catgatgaac ctgaatcgcc
     1681 agcggcatca gcaccttgtc gccttgcgta taatatttgc ccatggtgaa aacgggggcg
     1741 aagaagttgt ccatattggc cacgtttaaa tcaaaactgg tgaaactcac ccagggattg
     1801 gctgaaacga aaaacatatt ctcaataaac cctttaggga aataggccag gttttcaccg
     1861 taacacgcca catcttg
//