LOCUS       pEW3:_pET-32a_Trx-6xHis 7133 bp ds-DNA     circular     30-AUG-2018
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    1724..1813
                     /label="K-rich"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     rep_origin      2042..2348
                     /label="F1 ori"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     misc_feature    464..481
                     /label="Thrombin"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     affinity        437..454
                     /label="6x His Tag"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     rep_origin      3567..4249
                     /label="ColE1 origin"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     misc_feature    569..1813
                     /label="Dot1L"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    548..568
                     /label="TEV"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    485..532
                     /label="S-tag"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     affinity        1857..1874
                     /label="6x His Tag"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    89..415
                     /label="Trx-Tag"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    1646..1699
                     /label="Bat3_interacting"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     rep_origin      2025..2480
                     /label="M13 origin"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     misc_feature    578..1561
                     /label="Structured"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     CDS             2810..3469
                     /label="AmpR"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     primer_bind     1..20
                     /label="T7"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    569..1813
                     /label="Catalytic domain"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_binding    20..42
                     /label="LacO"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    75..80
                     /label="RBS"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     CDS             complement(5664..6620)
                     /label="LacI"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
ORIGIN
        1 taatacgact cactataggg gaattgtgag cggataacaa ttcccctcta gaaataattt
       61 tgtttaactt taagaaggag atatacatat gagcgataaa attattcacc tgactgacga
      121 cagttttgac acggatgtac tcaaagcgga cggggcgatc ctcgtcgatt tctgggcaga
      181 gtggtgcggt ccgtgcaaaa tgatcgcccc gattctggat gaaatcgctg acgaatatca
      241 gggcaaactg accgttgcaa aactgaacat cgatcaaaac cctggcactg cgccgaaata
      301 tggcatccgt ggtatcccga ctctgctgct gttcaaaaac ggtgaagtgg cggcaaccaa
      361 agtgggtgca ctgtctaaag gtcagttgaa agagttcctc gacgctaacc tggccggttc
      421 tggttctggc catatgcacc atcatcatca tcattcttct ggtctggtgc cacgcggttc
      481 tggtatgaaa gaaaccgctg ctgctaaatt cgaacgccag cacatggaca gcccagatct
      541 gggtaccGAA AACCTGTATT TTCAGGGCGG GGAGAAGCTG GAGCTGAGAC TGAAGTCGCC
      601 CGTGGGGGCT GAGCCCGCCG TCTACCCGTG GCCGCTGCCG GTCTACGATA AACATCACGA
      661 TGCTGCTCAT GAAATCATCG AGACCATCCG ATGGGTCTGT GAAGAAATCC CGGATCTCAA
      721 GCTCGCTATG GAGAATTACG TTTTAATTGA CTATGACACC AAAAGCTTCG AGAGCATGCA
      781 GAGGCTCTGC GACAAGTACA ACCGTGCCAT CGACAGCATC CACCAGCTGT GGAAGGGCAC
      841 CACGCAGCCC ATGAAGCTGA ACACGCGGCC GTCCACTGGA CTCCTGCGCC ATATCCTGCA
      901 GCAGGTCTAC AACCACTCGG TGACCGACCC CGAGAAGCTC AACAACTACG AGCCCTTCTC
      961 CCCCGAGGTG TACGGGGAGA CCTCCTTCGA CCTGGTGGCC CAGATGATTG ATGAGATCAA
     1021 GATGACCGAC GACGACCTGT TTGTGGACTT GGGGAGCGGT GTGGGCCAGG TCGTGCTCCA
     1081 GGTTGCTGCT GCCACCAACT GCAAACATCA CTATGGCGTC GAGAAAGCAG ACATCCCGGC
     1141 CAAGTATGCG GAGACCATGG ACCGCGAGTT CAGGAAGTGG ATGAAATGGT ATGGAAAAAA
     1201 GCATGCAGAA TACACATTGG AGAGAGGCGA TTTCCTCTCA GAAGAGTGGA GGGAGCGAAT
     1261 CGCCAACACG AGTGTTATAT TTGTGAATAA TTTTGCCTTT GGTCCTGAGG TGGATCACCA
     1321 GCTGAAGGAG CGGTTTGCAA ACATGAAGGA AGGTGGCAGA ATCGTGTCCT CGAAACCCTT
     1381 TGCACCTCTG AACTTCAGAA TAAACAGTAG AAACTTGAGT GACATCGGCA CCATCATGCG
     1441 CGTGGTGGAG CTCTCGCCCC TGAAGGGCTC GGTGTCGTGG ACGGGGAAGC CAGTCTCCTA
     1501 CTACCTGCAC ACTATCGACC GCACCATACT TGAAAACTAT TTTTCTAGTC TGAAAAACCC
     1561 AAAACTCAGG GAGGAACAGG AGGCAGCCCG GCGCCGCCAG CAGCGCGAGA GCAAGAGCAA
     1621 CGCGGCCACG CCCACTAAGG GCCCAGAGGG CAAGGTGGCC GGCCCCGCCG ACGCCCCCAT
     1681 GGACTCTGGT GCTGAGGAAG AGAAGGCGGG AGCAGCCACC GTGAAGAAGC CGTCTCCCTC
     1741 CAAAGCCCGC AAGAAGAAGC TAAACAAGAA GGGGAGGAAG ATGGCTGGCC GCAAGCGCGG
     1801 GCGCCCCAAG AAGtaagaat tcgagctccg tcgacaagct tgcggccgca ctcgagcacc
     1861 accaccacca ccactgagat ccggctgcta acaaagcccg aaaggaagct gagttggctg
     1921 ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg
     1981 gttttttgct gaaaggagga actatatccg gattggcgaa tgggacgcgc cctgtagcgg
     2041 cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc
     2101 cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc
     2161 ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct
     2221 cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac
     2281 ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac
     2341 tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat
     2401 ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa
     2461 aatattaacg tttacaattt caggtggcac ttttcgggga aatgtgcgcg gaacccctat
     2521 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata
     2581 aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct
     2641 tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa
     2701 agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa
     2761 cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt
     2821 taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg
     2881 tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca
     2941 tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa
     3001 cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt
     3061 gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc
     3121 cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa
     3181 actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga
     3241 ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc
     3301 tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga
     3361 tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga
     3421 acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga
     3481 ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat
     3541 ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt
     3601 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
     3661 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
     3721 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
     3781 aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
     3841 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
     3901 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
     3961 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
     4021 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
     4081 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
     4141 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
     4201 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
     4261 cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt
     4321 ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga
     4381 gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac
     4441 gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat
     4501 gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc
     4561 cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg
     4621 cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat
     4681 caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac
     4741 agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct
     4801 ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct
     4861 ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc
     4921 tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac
     4981 aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct
     5041 tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc
     5101 ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac
     5161 cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg
     5221 ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc
     5281 gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ggccgccatg ccggcgataa
     5341 tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg
     5401 cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc
     5461 ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa
     5521 agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga
     5581 ctgggttgaa ggctctcaag ggcatcggtc gagatcccgg tgcctaatga gtgagctaac
     5641 ttacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc
     5701 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgccagggtg
     5761 gtttttcttt tcaccagtga gacgggcaac agctgattgc ccttcaccgc ctggccctga
     5821 gagagttgca gcaagcggtc cacgctggtt tgccccagca ggcgaaaatc ctgtttgatg
     5881 gtggttaacg gcgggatata acatgagctg tcttcggtat cgtcgtatcc cactaccgag
     5941 atgtccgcac caacgcgcag cccggactcg gtaatggcgc gcattgcgcc cagcgccatc
     6001 tgatcgttgg caaccagcat cgcagtggga acgatgccct cattcagcat ttgcatggtt
     6061 tgttgaaaac cggacatggc actccagtcg ccttcccgtt ccgctatcgg ctgaatttga
     6121 ttgcgagtga gatatttatg ccagccagcc agacgcagac gcgccgagac agaacttaat
     6181 gggcccgcta acagcgcgat ttgctggtga cccaatgcga ccagatgctc cacgcccagt
     6241 cgcgtaccgt cttcatggga gaaaataata ctgttgatgg gtgtctggtc agagacatca
     6301 agaaataacg ccggaacatt agtgcaggca gcttccacag caatggcatc ctggtcatcc
     6361 agcggatagt taatgatcag cccactgacg cgttgcgcga gaagattgtg caccgccgct
     6421 ttacaggctt cgacgccgct tcgttctacc atcgacacca ccacgctggc acccagttga
     6481 tcggcgcgag atttaatcgc cgcgacaatt tgcgacggcg cgtgcagggc cagactggag
     6541 gtggcaacgc caatcagcaa cgactgtttg cccgccagtt gttgtgccac gcggttggga
     6601 atgtaattca gctccgccat cgccgcttcc actttttccc gcgttttcgc agaaacgtgg
     6661 ctggcctggt tcaccacgcg ggaaacggtc tgataagaga caccggcata ctctgcgaca
     6721 tcgtataacg ttactggttt cacattcacc accctgaatt gactctcttc cgggcgctat
     6781 catgccatac cgcgaaaggt tttgcgccat tcgatggtgt ccgggatctc gacgctctcc
     6841 cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc
     6901 cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc
     6961 tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc
     7021 cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc
     7081 ggccacgatg cgtccggcgt agaggatcga gatcgatctc gatcccgcga aat
//