LOCUS       pEW4:_pET-32a_Trx-6xHis 7043 bp ds-DNA     circular     08-FEB-2017
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    89..415
                     /label="Trx-Tag"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     affinity        437..454
                     /label="6x His Tag"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    548..568
                     /label="TEV"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    578..1561
                     /label="Structured"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    75..80
                     /label="RBS"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     misc_feature    464..481
                     /label="Thrombin"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     primer_bind     1..20
                     /label="T7"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     CDS             complement(5574..6530)
                     /label="LacI"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     misc_feature    1646..1699
                     /label="Bat3_interacting"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     rep_origin      3477..4159
                     /label="ColE1 origin"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     rep_origin      1935..2390
                     /label="M13 origin"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     CDS             2720..3379
                     /label="AmpR"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_binding    20..42
                     /label="LacO"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     affinity        1767..1784
                     /label="6x His Tag"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     rep_origin      1952..2258
                     /label="F1 ori"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     misc_feature    569..1723
                     /label="Dot1L"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    569..1723
                     /label="Catalytic domain"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    485..532
                     /label="S-tag"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
ORIGIN
        1 taatacgact cactataggg gaattgtgag cggataacaa ttcccctcta gaaataattt
       61 tgtttaactt taagaaggag atatacatat gagcgataaa attattcacc tgactgacga
      121 cagttttgac acggatgtac tcaaagcgga cggggcgatc ctcgtcgatt tctgggcaga
      181 gtggtgcggt ccgtgcaaaa tgatcgcccc gattctggat gaaatcgctg acgaatatca
      241 gggcaaactg accgttgcaa aactgaacat cgatcaaaac cctggcactg cgccgaaata
      301 tggcatccgt ggtatcccga ctctgctgct gttcaaaaac ggtgaagtgg cggcaaccaa
      361 agtgggtgca ctgtctaaag gtcagttgaa agagttcctc gacgctaacc tggccggttc
      421 tggttctggc catatgcacc atcatcatca tcattcttct ggtctggtgc cacgcggttc
      481 tggtatgaaa gaaaccgctg ctgctaaatt cgaacgccag cacatggaca gcccagatct
      541 gggtaccGAA AACCTGTATT TTCAGGGCGG GGAGAAGCTG GAGCTGAGAC TGAAGTCGCC
      601 CGTGGGGGCT GAGCCCGCCG TCTACCCGTG GCCGCTGCCG GTCTACGATA AACATCACGA
      661 TGCTGCTCAT GAAATCATCG AGACCATCCG ATGGGTCTGT GAAGAAATCC CGGATCTCAA
      721 GCTCGCTATG GAGAATTACG TTTTAATTGA CTATGACACC AAAAGCTTCG AGAGCATGCA
      781 GAGGCTCTGC GACAAGTACA ACCGTGCCAT CGACAGCATC CACCAGCTGT GGAAGGGCAC
      841 CACGCAGCCC ATGAAGCTGA ACACGCGGCC GTCCACTGGA CTCCTGCGCC ATATCCTGCA
      901 GCAGGTCTAC AACCACTCGG TGACCGACCC CGAGAAGCTC AACAACTACG AGCCCTTCTC
      961 CCCCGAGGTG TACGGGGAGA CCTCCTTCGA CCTGGTGGCC CAGATGATTG ATGAGATCAA
     1021 GATGACCGAC GACGACCTGT TTGTGGACTT GGGGAGCGGT GTGGGCCAGG TCGTGCTCCA
     1081 GGTTGCTGCT GCCACCAACT GCAAACATCA CTATGGCGTC GAGAAAGCAG ACATCCCGGC
     1141 CAAGTATGCG GAGACCATGG ACCGCGAGTT CAGGAAGTGG ATGAAATGGT ATGGAAAAAA
     1201 GCATGCAGAA TACACATTGG AGAGAGGCGA TTTCCTCTCA GAAGAGTGGA GGGAGCGAAT
     1261 CGCCAACACG AGTGTTATAT TTGTGAATAA TTTTGCCTTT GGTCCTGAGG TGGATCACCA
     1321 GCTGAAGGAG CGGTTTGCAA ACATGAAGGA AGGTGGCAGA ATCGTGTCCT CGAAACCCTT
     1381 TGCACCTCTG AACTTCAGAA TAAACAGTAG AAACTTGAGT GACATCGGCA CCATCATGCG
     1441 CGTGGTGGAG CTCTCGCCCC TGAAGGGCTC GGTGTCGTGG ACGGGGAAGC CAGTCTCCTA
     1501 CTACCTGCAC ACTATCGACC GCACCATACT TGAAAACTAT TTTTCTAGTC TGAAAAACCC
     1561 AAAACTCAGG GAGGAACAGG AGGCAGCCCG GCGCCGCCAG CAGCGCGAGA GCAAGAGCAA
     1621 CGCGGCCACG CCCACTAAGG GCCCAGAGGG CAAGGTGGCC GGCCCCGCCG ACGCCCCCAT
     1681 GGACTCTGGT GCTGAGGAAG AGAAGGCGGG AGCAGCCACC GTGtaagaat tcgagctccg
     1741 tcgacaagct tgcggccgca ctcgagcacc accaccacca ccactgagat ccggctgcta
     1801 acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac
     1861 cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg
     1921 gattggcgaa tgggacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac
     1981 gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc
     2041 ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt
     2101 agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg
     2161 ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac
     2221 gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta
     2281 ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat
     2341 ttaacaaaaa tttaacgcga attttaacaa aatattaacg tttacaattt caggtggcac
     2401 ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat
     2461 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag
     2521 tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc
     2581 tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc
     2641 acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc
     2701 cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc
     2761 ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt
     2821 ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt
     2881 atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat
     2941 cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct
     3001 tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat
     3061 gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc
     3121 ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg
     3181 ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc
     3241 tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta
     3301 cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc
     3361 ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga
     3421 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat
     3481 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
     3541 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     3601 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     3661 ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt
     3721 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     3781 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     3841 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
     3901 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
     3961 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
     4021 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
     4081 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
     4141 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
     4201 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc
     4261 tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga
     4321 agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata
     4381 tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc
     4441 tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc
     4501 cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga
     4561 gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa
     4621 gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct
     4681 cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg
     4741 cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg
     4801 taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg
     4861 cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga
     4921 gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg
     4981 gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc
     5041 gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg
     5101 cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct
     5161 aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc
     5221 gcacccgtgg ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg
     5281 cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca
     5341 ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg
     5401 ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag
     5461 tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc
     5521 gagatcccgg tgcctaatga gtgagctaac ttacattaat tgcgttgcgc tcactgcccg
     5581 ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga
     5641 gaggcggttt gcgtattggg cgccagggtg gtttttcttt tcaccagtga gacgggcaac
     5701 agctgattgc ccttcaccgc ctggccctga gagagttgca gcaagcggtc cacgctggtt
     5761 tgccccagca ggcgaaaatc ctgtttgatg gtggttaacg gcgggatata acatgagctg
     5821 tcttcggtat cgtcgtatcc cactaccgag atgtccgcac caacgcgcag cccggactcg
     5881 gtaatggcgc gcattgcgcc cagcgccatc tgatcgttgg caaccagcat cgcagtggga
     5941 acgatgccct cattcagcat ttgcatggtt tgttgaaaac cggacatggc actccagtcg
     6001 ccttcccgtt ccgctatcgg ctgaatttga ttgcgagtga gatatttatg ccagccagcc
     6061 agacgcagac gcgccgagac agaacttaat gggcccgcta acagcgcgat ttgctggtga
     6121 cccaatgcga ccagatgctc cacgcccagt cgcgtaccgt cttcatggga gaaaataata
     6181 ctgttgatgg gtgtctggtc agagacatca agaaataacg ccggaacatt agtgcaggca
     6241 gcttccacag caatggcatc ctggtcatcc agcggatagt taatgatcag cccactgacg
     6301 cgttgcgcga gaagattgtg caccgccgct ttacaggctt cgacgccgct tcgttctacc
     6361 atcgacacca ccacgctggc acccagttga tcggcgcgag atttaatcgc cgcgacaatt
     6421 tgcgacggcg cgtgcagggc cagactggag gtggcaacgc caatcagcaa cgactgtttg
     6481 cccgccagtt gttgtgccac gcggttggga atgtaattca gctccgccat cgccgcttcc
     6541 actttttccc gcgttttcgc agaaacgtgg ctggcctggt tcaccacgcg ggaaacggtc
     6601 tgataagaga caccggcata ctctgcgaca tcgtataacg ttactggttt cacattcacc
     6661 accctgaatt gactctcttc cgggcgctat catgccatac cgcgaaaggt tttgcgccat
     6721 tcgatggtgt ccgggatctc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc
     6781 cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat
     6841 ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct
     6901 catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc
     6961 agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga
     7021 gatcgatctc gatcccgcga aat
//