LOCUS       pQE30-His-AlaRS         6056 bp ds-DNA     circular     18-FEB-2019
DEFINITION  .
KEYWORDS    "YTK Part Number" "Resistance" "Bacterial Origin" "Yeast Origin"   
            "Yeast Marker" "Box" "Position" "Date Made" "creator:Barbora"
            "marker:AmpR, CmR"
FEATURES             Location/Qualifiers
     promoter        complement(5852..5956)
                     /label="AmpR promoter"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     CDS             complement(5783..5851)
                     /label="AmpR"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     CDS             127..144
                     /label="6xHis"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     rep_origin      complement(4232..4820)
                     /label="ori"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     promoter        10..24
                     /label="T5 promoter"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     promoter        31..47
                     /label="T5 promoter"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     terminator      2803..2897
                     /label="lambda t0 terminator"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     promoter        25..30
                     /label="T5 promoter"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     protein_bind    62..78
                     /label="lac operator"
                     /ApEinfo_revcolor=#31849b
                     /ApEinfo_fwdcolor=#31849b
     promoter        48..54
                     /label="T5 promoter"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    3906..4046
                     /label="bom"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    151..2782
                     /label="AlaRS"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     CDS             complement(4991..5782)
                     /label="AmpR"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     CDS             2941..3600
                     /label="CmR"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     RBS             97..108
                     /label="RBS"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     terminator      3665..3751
                     /label="rrnB T1 terminator"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
ORIGIN
        1 CTCGAGAAAT CATAAAAAAT TTATTTGCTT TGTGAGCGGA TAACAATTAT AATAGATTCA
       61 ATTGTGAGCG GATAACAATT TCACACAGAA TTCATTAAAG AGGAGAAATT AACTATGAGA
      121 GGATCGCATC ACCATCACCA TCACGGATCC ATGAGCAACA GCACCGCTGA GATCCGTCAG
      181 GCGTTTCTCG ACTTTTTCCA TAGTAAGGGA CATCAGGTAG TTGCCAGCAG CTCCCTGGTA
      241 CCCCATAACG ACCCAACTTT GTTGTTTACC AACGCCGGGA TGAACCAGTT CAAGGATGTG
      301 TTCCTTGGGC TCGACAAGCG TAATTATTCC CGCGCTACCA CTTCCCAACG CTGCGTGCGT
      361 GCGGGTGGTA AACACAACGA CCTGGAAAAC GTCGGTTACA CCGCGCGTCA CCATACCTTC
      421 TTCGAAATGC TGGGCAACTT CAGCTTCGGC GACTATTTCA AACACGATGC CATTCAGTTT
      481 GCATGGGAAC TGCTGACCAG CGAAAAATGG TTTGCCCTGC CGAAAGAGCG TCTGTGGGTT
      541 ACCGTCTATG AAAGCGACGA CGAAGCCTAC GAAATCTGGG AAAAAGAAGT AGGGATCCCG
      601 CGCGAACGTA TTATTCGCAT CGGCGATAAC AAAGGTGCGC CATACGCATC TGACAACTTC
      661 TGGCAGATGG GTGACACTGG TCCGTGCGGC CCGTGCACCG AAATCTTCTA CGATCACGGC
      721 GACCACATTT GGGGGGGCCC TCCGGGAAGC CCGGAAGAAG ACGGCGACCG CTACATTGAG
      781 ATCTGGAACA TCGTCTTCAT GCAGTTCAAC CGCCAGGCCG ATGGCACGAT GGAACCGCTG
      841 CCGAAGCCGT CTGTAGATAC CGGTATGGGT CTGGAGCGTA TTGCTGCGGT GCTGCAACAC
      901 GTTAACTCTA ACTATGACAT CGACCTGTTC CGCACGCTGA TCCAGGCGGT AGCGAAAGTC
      961 ACTGGCGCAA CCGATCTGAG CAATAAATCG CTGCGCGTAA TCGCTGACCA CATTCGTTCT
     1021 TGTGCGTTCC TGATCGCGGA TGGCGTAATG CCGTCCAATG AAAACCGTGG TTATGTACTG
     1081 CGTCGTATCA TTCGTCGCGC AGTGCGTCAC GGTAATATGC TCGGCGCGAA AGAAACCTTC
     1141 TTCTACAAAC TGGTTGGTCC GCTGATCGAC GTTATGGGCT CTGCGGGTGA AGACCTGAAA
     1201 CGCCAGCAGG CGCAGGTTGA GCAGGTGCTG AAGACTGAAG AAGAGCAGTT TGCTCGTACT
     1261 CTGGAGCGCG GTCTGGCGTT GCTGGATGAA GAGCTGGCAA AACTTTCTGG TGATACGCTG
     1321 GATGGTGAAA CTGCTTTCCG TCTGTACGAC ACCTATGGCT TCCCGGTTGA CCTGACGGCT
     1381 GATGTTTGTC GTGAGCGCAA CATCAAAGTT GACGAAGCTG GTTTTGAAGC TGCAATGGAA
     1441 GAGCAGCGTC GTCGCGCGCG CGAAGCCAGC GGCTTTGGTG CCGATTACAA CGCAATGATC
     1501 CGTGTTGACA GTGCATCTGA ATTTAAAGGC TATGACCATC TGGAACTGAA CGGCAAAGTG
     1561 ACTGCGCTGT TTGTTGATGG TAAAGCGGTT GATGCCATCA ATGCAGGCCA GGAAGCTGTG
     1621 GTCGTGCTGG ATCAAACGCC ATTCTATGCG GAATCCGGCG GTCAGGTTGG CGATAAAGGC
     1681 GAACTGAAAG GCGCTAACTT CTCCTTTGCG GTGGAAGATA CGCAGAAATA CGGCCAGGCG
     1741 ATTGGTCACA TCGGTAAACT TGCTGCGGGT TCTCTGAAAG TGGGCGACGC GGTGCAGGCT
     1801 GATGTTGATG AGGCTCGTCG CGCCCGTATT CGTCTGAATC ACTCCGCAAC GCACCTGATG
     1861 CACGCTGCGC TGCGCCAGGT TCTGGGTACT CATGTATCGC AGAAAGGTTC ACTGGTTAAC
     1921 GACAAGGTGC TGCGCTTCGA CTTCTCACAC AACGAAGCGA TGAAACCAGA AGAGATTCGT
     1981 GCGGTCGAAG ACCTGGTGAA CACACAGATT CGTCGCAATT TGCCGATCGA AACCAACATC
     2041 ATGGATCTCG AAGCGGCGAA AGCGAAAGGT GCGATGGCGC TGTTCGGCGA GAAGTATGAT
     2101 GAGCGCGTAC GCGTGCTGAG CATGGGCGAT TTCTCTACCG AGTTGTGTGG CGGTACTCAC
     2161 GCCAGCCGCA CTGGTGATAT TGGTCTGTTC CGCATCATCT CTGAATCGGG TACTGCTGCA
     2221 GGCGTTCGTC GTATCGAAGC GGTAACCGGA GAAGGTGCTA TCGCCACCGT TCATGCAGAC
     2281 AGCGATCGCT TAAGCGAAGT CGCGCATCTG CTGAAAGGCG ATAGCAATAA TCTGGCTGAT
     2341 AAAGTGCGCT CAGTACTGGA ACGTACGCGT CAGCTGGAAA AAGAGTTACA ACAGCTTAAA
     2401 GAACAAGCTG CCGCACAGGA GAGCGCAAAT CTTTCCAGTA AGGCAATTGA TGTTAATGGT
     2461 GTTAAGCTGT TGGTTAGCGA GCTTAGCGGT GTTGAGCCGA AAATGTTGCG TACCATGGTT
     2521 GACGATTTAA AAAATCAGCT GGGGTCGACA ATTATCGTGC TGGCAACGGT AGTCGAAGGT
     2581 AAGGTTTCTC TGATTGCAGG CGTATCTAAG GACGTCACAG ATCGTGTGAA AGCAGGGGAA
     2641 CTGATTGGTA TGGTCGCTCA GCAGGTGGGC GGCAAGGGTG GTGGACGTCC TGACATGGCG
     2701 CAAGCCGGTG GTACGGATGC TGCGGCCTTA CCTGCAGCGT TAGCCAGTGT GAAAGGCTGG
     2761 GTCAGCGCGA AATTGCAATA AAAGCTTAAT TAGCTGAGCT TGGACTCCTG TTGATAGATC
     2821 CAGTAATGAC CTCAGAACTC CATCTGGATT TGTTCAGAAC GCTCGGTTGC CGCCGGGCGT
     2881 TTTTTATTGG TGAGAATCCA AGCTAGCTTG GCGAGATTTT CAGGAGCTAA GGAAGCTAAA
     2941 ATGGAGAAAA AAATCACTGG ATATACCACC GTTGATATAT CCCAATGGCA TCGTAAAGAA
     3001 CATTTTGAGG CATTTCAGTC AGTTGCTCAA TGTACCTATA ACCAGACCGT TCAGCTGGAT
     3061 ATTACGGCCT TTTTAAAGAC CGTAAAGAAA AATAAGCACA AGTTTTATCC GGCCTTTATT
     3121 CACATTCTTG CCCGCCTGAT GAATGCTCAT CCGGAATTTC GTATGGCAAT GAAAGACGGT
     3181 GAGCTGGTGA TATGGGATAG TGTTCACCCT TGTTACACCG TTTTCCATGA GCAAACTGAA
     3241 ACGTTTTCAT CGCTCTGGAG TGAATACCAC GACGATTTCC GGCAGTTTCT ACACATATAT
     3301 TCGCAAGATG TGGCGTGTTA CGGTGAAAAC CTGGCCTATT TCCCTAAAGG GTTTATTGAG
     3361 AATATGTTTT TCGTCTCAGC CAATCCCTGG GTGAGTTTCA CCAGTTTTGA TTTAAACGTG
     3421 GCCAATATGG ACAACTTCTT CGCCCCCGTT TTCACCATGG GCAAATATTA TACGCAAGGC
     3481 GACAAGGTGC TGATGCCGCT GGCGATTCAG GTTCATCATG CCGTTTGTGA TGGCTTCCAT
     3541 GTCGGCAGAA TGCTTAATGA ATTACAACAG TACTGCGATG AGTGGCAGGG CGGGGCGTAA
     3601 TTTTTTTAAG GCAGTTATTG GTGCCCTTAA ACGCCTGGGG TAATGACTCT CTAGCTTGAG
     3661 GCATCAAATA AAACGAAAGG CTCAGTCGAA AGACTGGGCC TTTCGTTTTA TCTGTTGTTT
     3721 GTCGGTGAAC GCTCTCCTGA GTAGGACAAA TCCGCCCTCT AGAGCTGCCT CGCGCGTTTC
     3781 GGTGATGACG GTGAAAACCT CTGACACATG CAGCTCCCGG AGACGGTCAC AGCTTGTCTG
     3841 TAAGCGGATG CCGGGAGCAG ACAAGCCCGT CAGGGCGCGT CAGCGGGTGT TGGCGGGTGT
     3901 CGGGGCGCAG CCATGACCCA GTCACGTAGC GATAGCGGAG TGTATACTGG CTTAACTATG
     3961 CGGCATCAGA GCAGATTGTA CTGAGAGTGC ACCATATGCG GTGTGAAATA CCGCACAGAT
     4021 GCGTAAGGAG AAAATACCGC ATCAGGCGCT CTTCCGCTTC CTCGCTCACT GACTCGCTGC
     4081 GCTCGGTCGT TCGGCTGCGG CGAGCGGTAT CAGCTCACTC AAAGGCGGTA ATACGGTTAT
     4141 CCACAGAATC AGGGGATAAC GCAGGAAAGA ACATGTGAGC AAAAGGCCAG CAAAAGGCCA
     4201 GGAACCGTAA AAAGGCCGCG TTGCTGGCGT TTTTCCATAG GCTCCGCCCC CCTGACGAGC
     4261 ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC
     4321 AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG
     4381 GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC TCACGCTGTA
     4441 GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCG
     4501 TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC CCGGTAAGAC
     4561 ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG AGGTATGTAG
     4621 GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA AGGACAGTAT
     4681 TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT
     4741 CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG CAGATTACGC
     4801 GCAGAAAAAA AGGATCTCAA GAAGATCCTT TGATCTTTTC TACGGGGTCT GACGCTCAGT
     4861 GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGAGATT ATCAAAAAGG ATCTTCACCT
     4921 AGATCCTTTT AAATTAAAAA TGAAGTTTTA AATCAATCTA AAGTATATAT GAGTAAACTT
     4981 GGTCTGACAG TTACCAATGC TTAATCAGTG AGGCACCTAT CTCAGCGATC TGTCTATTTC
     5041 GTTCATCCAT AGTTGCCTGA CTCCCCGTCG TGTAGATAAC TACGATACGG GAGGGCTTAC
     5101 CATCTGGCCC CAGTGCTGCA ATGATACCGC GAGACCCACG CTCACCGGCT CCAGATTTAT
     5161 CAGCAATAAA CCAGCCAGCC GGAAGGGCCG AGCGCAGAAG TGGTCCTGCA ACTTTATCCG
     5221 CCTCCATCCA GTCTATTAAT TGTTGCCGGG AAGCTAGAGT AAGTAGTTCG CCAGTTAATA
     5281 GTTTGCGCAA CGTTGTTGCC ATTGCTACAG GCATCGTGGT GTCACGCTCG TCGTTTGGTA
     5341 TGGCTTCATT CAGCTCCGGT TCCCAACGAT CAAGGCGAGT TACATGATCC CCCATGTTGT
     5401 GCAAAAAAGC GGTTAGCTCC TTCGGTCCTC CGATCGTTGT CAGAAGTAAG TTGGCCGCAG
     5461 TGTTATCACT CATGGTTATG GCAGCACTGC ATAATTCTCT TACTGTCATG CCATCCGTAA
     5521 GATGCTTTTC TGTGACTGGT GAGTACTCAA CCAAGTCATT CTGAGAATAG TGTATGCGGC
     5581 GACCGAGTTG CTCTTGCCCG GCGTCAATAC GGGATAATAC CGCGCCACAT AGCAGAACTT
     5641 TAAAAGTGCT CATCATTGGA AAACGTTCTT CGGGGCGAAA ACTCTCAAGG ATCTTACCGC
     5701 TGTTGAGATC CAGTTCGATG TAACCCACTC GTGCACCCAA CTGATCTTCA GCATCTTTTA
     5761 CTTTCACCAG CGTTTCTGGG TGAGCAAAAA CAGGAAGGCA AAATGCCGCA AAAAAGGGAA
     5821 TAAGGGCGAC ACGGAAATGT TGAATACTCA TACTCTTCCT TTTTCAATAT TATTGAAGCA
     5881 TTTATCAGGG TTATTGTCTC ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC
     5941 AAATAGGGGT TCCGCGCACA TTTCCCCGAA AAGTGCCACC TGACGTCTAA GAAACCATTA
     6001 TTATCATGAC ATTAACCTAT AAAAATAGGC GTATCACGAG GCCCTTTCGT CTTCAC
//