LOCUS       pET16b-His-ArgRS        7827 bp ds-DNA     circular     19-FEB-2019
DEFINITION  .
KEYWORDS    "creator:Barbora" "marker:AmpR"                                    
FEATURES             Location/Qualifiers
     RBS             5220..5242
                     /label="RBS"
     misc_feature    complement(3417..5150)
                     /label="ArgRS"
     CDS             complement(5154..5165)
                     /label="Factor Xa site"
     promoter        5609..5686
                     /label="lacI promoter"
     rep_origin      complement(875..1463)
                     /label="ori"
     CDS             254..445
                     /label="rop"
     CDS             complement(1634..2425)
                     /label="AmpR"
     promoter        complement(5282..5300)
                     /label="T7 promoter"
     CDS             complement(2426..2494)
                     /label="AmpR"
     promoter        2718..2734
                     /label="tet promoter"
     misc_feature    547..689
                     /label="bom"
     terminator      2915..2962
                     /label="T7 terminator"
     promoter        2712..2717
                     /label="tet promoter"
     protein_bind    5257..5281
                     /label="lac operator"
     CDS             complement(1637..2296)
                     /label="AmpR"
     CDS             complement(5178..5207)
                     /label="10xHis"
     CDS             5687..6769
                     /label="lacI"
     rep_origin      complement(857..1539)
                     /label="ColE1 origin"
     primer_bind     complement(5281..5300)
                     /label="T7"
     promoter        complement(2495..2599)
                     /label="AmpR promoter"
     misc_binding    complement(5259..5281)
                     /label="LacO"
     promoter        2735..2740
                     /label="tet promoter"
ORIGIN
        1 gttccggatc tgcatcgcag gatgctgctg gctaccctgt ggaacaccta catctgtatt
       61 aacgaagcgc tggcattgac cctgagtgat ttttctctgg tcccgccgca tccataccgc
      121 cagttgttta ccctcacaac gttccagtaa ccgggcatgt tcatcatcag taacccgtat
      181 cgtgagcatc ctctctcgtt tcatcggtat cattaccccc atgaacagaa atccccctta
      241 cacggaggca tcagtgacca aacaggaaaa aaccgccctt aacatggccc gctttatcag
      301 aagccagaca ttaacgcttc tggagaaact caacgagctg gacgcggatg aacaggcaga
      361 catctgtgaa tcgcttcacg accacgctga tgagctttac cgcagctgcc tcgcgcgttt
      421 cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca cagcttgtct
      481 gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg
      541 tcggggcgca gccatgaccc agtcacgtag cgatagcgga gtgtatactg gcttaactat
      601 gcggcatcag agcagattgt actgagagtg caccatatat gcggtgtgaa ataccgcaca
      661 gatgcgtaag gagaaaatac cgcatcaggc gctcttccgc ttcctcgctc actgactcgc
      721 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
      781 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
      841 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
      901 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
      961 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
     1021 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
     1081 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
     1141 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
     1201 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
     1261 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag
     1321 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
     1381 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
     1441 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
     1501 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
     1561 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
     1621 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
     1681 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
     1741 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
     1801 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
     1861 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
     1921 atagtttgcg caacgttgtt gccattgctg caggcatcgt ggtgtcacgc tcgtcgtttg
     1981 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
     2041 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
     2101 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
     2161 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
     2221 ggcgaccgag ttgctcttgc ccggcgtcaa cacgggataa taccgcgcca catagcagaa
     2281 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
     2341 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
     2401 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
     2461 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
     2521 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
     2581 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc taagaaacca
     2641 ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtcttcaag
     2701 aattctcatg tttgacagct tatcatcgat aagctttaat gcggtagttt atcacagtta
     2761 aattgctaac gcagtcaggc accgtgtatg aaatctaaca atgcgctcat cgtcatcctc
     2821 ggcaccgtca ccctggatgc tgtaggcata ggcttggtta tgccggtact gccgggcctc
     2881 ttgcgggata tccggatata gttcctcctt tcagcaaaaa acccctcaag acccgtttag
     2941 aggccccaag gggttatgct agTTATTGCT CAGCGGTGGC AGCAGCCAAC TCAGCTTCCT
     3001 TTCGGGCTTT GTTAGCAGCC GGATCCAAGC TTCACCATAG GCTTTATTTA ATTGCGCATC
     3061 GACGAGATTG TATTCTTGTC GCAGGCATGA TTTGATCACG TTAACGTCAG TATCATCATC
     3121 TGAATTTTTT TGCCAACAAT CTTTGATGGC TGAAATGTCA TGGTCGTTTA CTATAACCGT
     3181 ATCTTCTGCG TAAGCCTGGC AAGAGATAAT GCCTAATGTT AATACAAGAA ATTTAAACAC
     3241 TGCGGTATTC CTTAAATTCA ACCGTTATCG TTTCCCTGGG GCCGGATATT ACGCTTCTGG
     3301 CCTCTTATCC AACAGTGAGA AAACAATGGC ATGTCCATTT TCAGAATACA TTTAGATGGC
     3361 AATAAAAAAG CCTGATCGGC TAACCCTGAT CAGGCTTCAC TCTCACGAAA AATCGATTAC
     3421 ATACGCTCTA CAGTCTCAAT ACCCAGCGTA TCCAGACCCA GCTTCAGCGT CTTCGCCGTC
     3481 AGTTGTGCCA GTTTTAGACG GCTGTTACGC ACTTCTTCGT TTTCTGCGCT GAGGATCGGG
     3541 CAGTGCTCGT AGAAGCCAGA GAACAGACCG GCCAGATCGT ACAGGTAAGC ACACATTACA
     3601 TGCGGCGTGC CTTCACGGGC AACCACGGTG AGGGTTTCTT CAAACTGCAG CAGGCGAGCT
     3661 GCCAGTTGCG CTTCACGATC TTCACGGATG ATAACCGGAG CTGCAGCCAG TTGCTCTTCG
     3721 TCAATTTCTG CTTTACGGAA CACGGACAAT ACACGCGTGT ATGCATACTG CATGTATGGC
     3781 GCGGTATTAC CCTCAAACGC CAGCATGTTG TCCCAGTCGA AGATGTAGTC CGTGGTGCGG
     3841 TTTTTGGAGA GATCCGCATA TTTCACCGCA CCAATACCAA CCGCGTTAGC CAGTTTTTCC
     3901 AGCTCGTCGG CTGGCATATC CGGGTTCTTT TCTGCCACCA GACGGCGTGC ACGTTCCAGG
     3961 GCTTCATCCA GCAGATCGGC CAGTTTCACT GTACCACCCG CGCGGGTTTT GAACGGTTTG
     4021 CCGTCTTTAC CCAGCATCAT GCCGAACATG TGGTGTTCCA GCGGTACGGA TTCCGGTACA
     4081 TAGCCTGCTT TACGGACGAT CGCCCATGCC TGCATCAGGT GTTGATGCTG ACGGGAGTCG
     4141 ATGTAATACA GCACGCGATC GGCATGCAGT GTTTCATAAC GATATTTCGC ACAGGCGATA
     4201 TCAGTGGTGG TGTAGAGATA GCCGCCATCT TTCTTCTGAA TGATCACGCC CATCGGTTCG
     4261 CCTTCCTTGT TTTTAAACTC ATCAAGGAAT ACGACGGTCG CCCCTTCGCT TTCTACTGCC
     4321 AGACCTTTGG CTTTGAGATC CGCCACAATT CCTGGCAGCA TCGGGTTGTA GAGGCTTTCG
     4381 CCCATCACGT CATCACGGGT CAGCGTCACG TTGAGACGAT CGTAGGTGAT CTGGTTCTGC
     4441 GTCATGGTGA TGTCGACCAG TTTGCGCCAC ATCTCGCGGA AATATTCGTC ACCGCTTTGC
     4501 AGTTTTACCA CGTAGTTACG TGCGCGCTCG GCGAACTCTT CATCTTCATC GTAATGCTTT
     4561 TTCGCATCGC GGTAGAAACC TTCAAGGTCA GCCAGCTCCA TTTCACCGGC GTTTTCCTGC
     4621 TGCTGCTTTT CCAGCCATGC AATCAGCATA CCGAACTGAG TGCCCCAGTC GCCGACGTGG
     4681 TTTGCGCGAA TCACTTTGTG ACCGAGGAAC TCCAGAGTAC GCACTGCTGC GTCACCAATA
     4741 ATGGTAGAGC GCAGGTGACC GACATGCATC TCTTTCGCCA CGTTTGGCGC AGAGTAGTCA
     4801 ACCACAATGG TCTGTTTTTC TGGCGTAGCA ACACCGAGAC GATCGGACGC CAGCGCCTGC
     4861 TGAACATGTT CAGCCAGGAA TGCCGGATCA AGGAAAATGT TGATAAAGCC TGGACCGGCG
     4921 ATCTCAACTT TGCTGGCGAT ACCGTTAAGA TCCAGATGAG TCAGCACCTG CTCTGCTAAT
     4981 TGTCGCGGTG CCATACCCAG TTTTTTAGCA ACTGCCATCA TGCCGTTAGC CTGATAGTCG
     5041 CCGAACTGAA CTTTTGCTGA CTGACGAACC TGCGGTTCGC AATCCGCAGG CGCGCCTGCC
     5101 GCAATCATGG CCTGACGGAC TTTTTCTGAG AGAAGAGCCT GAATATTCAt atgacgacct
     5161 tcgatatggc cgctgctgtg atgatgatga tgatgatgat gatgatggcc catggtatat
     5221 ctccttctta aagttaaaca aaattatttc tagaggggaa ttgttatccg ctcacaattc
     5281 ccctatagtg agtcgtatta atttcgcggg atcgagatct cgatcctcta cgccggacgc
     5341 atcgtggccg gcatcaccgg cgccacaggt gcggttgctg gcgcctatat cgccgacatc
     5401 accgatgggg aagatcgggc tcgccacttc gggctcatga gcgcttgttt cggcgtgggt
     5461 atggtggcag gccccgtggc cgggggactg ttgggcgcca tctccttgca tgcaccattc
     5521 cttgcggcgg cggtgctcaa cggcctcaac ctactactgg gctgcttcct aatgcaggag
     5581 tcgcataagg gagagcgtcg agatcccgga caccatcgaa tggcgcaaaa cctttcgcgg
     5641 tatggcatga tagcgcccgg aagagagtca attcagggtg gtgaatgtga aaccagtaac
     5701 gttatacgat gtcgcagagt atgccggtgt ctcttatcag accgtttccc gcgtggtgaa
     5761 ccaggccagc cacgtttctg cgaaaacgcg ggaaaaagtg gaagcggcga tggcggagct
     5821 gaattacatt cccaaccgcg tggcacaaca actggcgggc aaacagtcgt tgctgattgg
     5881 cgttgccacc tccagtctgg ccctgcacgc gccgtcgcaa attgtcgcgg cgattaaatc
     5941 tcgcgccgat caactgggtg ccagcgtggt ggtgtcgatg gtagaacgaa gcggcgtcga
     6001 agcctgtaaa gcggcggtgc acaatcttct cgcgcaacgc gtcagtgggc tgatcattaa
     6061 ctatccgctg gatgaccagg atgccattgc tgtggaagct gcctgcacta atgttccggc
     6121 gttatttctt gatgtctctg accagacacc catcaacagt attattttct cccatgaaga
     6181 cggtacgcga ctgggcgtgg agcatctggt cgcattgggt caccagcaaa tcgcgctgtt
     6241 agcgggccca ttaagttctg tctcggcgcg tctgcgtctg gctggctggc ataaatatct
     6301 cactcgcaat caaattcagc cgatagcgga acgggaaggc gactggagtg ccatgtccgg
     6361 ttttcaacaa accatgcaaa tgctgaatga gggcatcgtt cccactgcga tgctggttgc
     6421 caacgatcag atggcgctgg gcgcaatgcg cgccattacc gagtccgggc tgcgcgttgg
     6481 tgcggatatc tcggtagtgg gatacgacga taccgaagac agctcatgtt atatcccgcc
     6541 gttaaccacc atcaaacagg attttcgcct gctggggcaa accagcgtgg accgcttgct
     6601 gcaactctct cagggccagg cggtgaaggg caatcagctg ttgcccgtct cactggtgaa
     6661 aagaaaaacc accctggcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc
     6721 attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa
     6781 ttaatgtaag ttagctcact cattaggcac cgggatctcg accgatgccc ttgagagcct
     6841 tcaacccagt cagctccttc cggtgggcgc ggggcatgac tatcgtcgcc gcacttatga
     6901 ctgtcttctt tatcatgcaa ctcgtaggac aggtgccggc agcgctctgg gtcattttcg
     6961 gcgaggaccg ctttcgctgg agcgcgacga tgatcggcct gtcgcttgcg gtattcggaa
     7021 tcttgcacgc cctcgctcaa gccttcgtca ctggtcccgc caccaaacgt ttcggcgaga
     7081 agcaggccat tatcgccggc atggcggccg acgcgctggg ctacgtcttg ctggcgttcg
     7141 cgacgcgagg ctggatggcc ttccccatta tgattcttct cgcttccggc ggcatcggga
     7201 tgcccgcgtt gcaggccatg ctgtccaggc aggtagatga cgaccatcag ggacagcttc
     7261 aaggatcgct cgcggctctt accagcctaa cttcgatcac tggaccgctg atcgtcacgg
     7321 cgatttatgc cgcctcggcg agcacatgga acgggttggc atggattgta ggcgccgccc
     7381 tataccttgt ctgcctcccc gcgttgcgtc gcggtgcatg gagccgggcc acctcgacct
     7441 gaatggaagc cggcggcacc tcgctaacgg attcaccact ccaagaattg gagccaatca
     7501 attcttgcgg agaactgtga atgcgcaaac caacccttgg cagaacatat ccatcgcgtc
     7561 cgccatctcc agcagccgca cgcggcgcat ctcgggcagc gttgggtcct ggccacgggt
     7621 gcgcatgatc gtgctcctgt cgttgaggac ccggctaggc tggcggggtt gccttactgg
     7681 ttagcagaat gaatcaccga tacgcgagcg aacgtgaagc gactgctgct gcaaaacgtc
     7741 tgcgacctga gcaacaacat gaatggtctt cggtttccgt gtttcgtaaa gtctggaaac
     7801 gcggaagtca gcgccctgca ccattat
//