LOCUS       9_pET-21a(+)            6639 bp ds-DNA     circular     14-FEB-2019
DEFINITION  .
KEYWORDS    "creator:Novagen (EMD Millipore)" "marker:AmpR"                    
            "organism:Escherichia coli"
FEATURES             Location/Qualifiers
     protein_bind    1480..1504
                     /label="lac operator"
     rep_origin      complement(4422..5010)
                     /label="ori"
     CDS             complement(5181..5972)
                     /label="AmpR"
     terminator      26..73
                     /label="T7 terminator"
     RBS             1444..1449
                     /label="RBS"
     CDS             complement(140..157)
                     /label="6xHis"
     misc_feature    complement(159..1435)
                     /label="HisRS"
     misc_feature    158..158
                     /label="MCS"
     CDS             complement(5973..6041)
                     /label="AmpR"
     CDS             3801..3992
                     /label="rop"
     promoter        1832..1909
                     /label="lacI promoter"
     promoter        complement(1505..1523)
                     /label="T7 promoter"
     CDS             1910..2992
                     /label="lacI"
     rep_origin      complement(6173..6628)
                     /label="f1 ori"
     promoter        complement(6042..6146)
                     /label="AmpR promoter"
ORIGIN
        1 ATCCGGATAT AGTTCCTCCT TTCAGCAAAA AACCCCTCAA GACCCGTTTA GAGGCCCCAA
       61 GGGGTTATGC TAGTTATTGC TCAGCGGTGG CAGCAGCCAA CTCAGCTTCC TTTCGGGCTT
      121 TGTTAGCAGC CGGATCTCAG TGGTGGTGGT GGTGGTGCTC GAGACCCAGT AACGTGCGCA
      181 AATGCGCGGC TACGCTATCC TGCGCAACTG CCGTTTGCTC ACCAGAGCGC AAATCCTTCA
      241 CTACTGCTGT GCCGTTAGCC ACTTCAGACT CACCCAGCAC CACAGCAACG CGGGCACCCC
      301 ATTTATCAGC ACGGGCAAAC TGTTTCTTAA AGTTGCCGCC GCCGTGGTTG GTCATCAATT
      361 TCACGCCCGG TAATTCATCA CGCAGACGCT CAGCTAATGC CATAGCCGCA GATTGTGTAT
      421 CAGCACCTGA AGCCACCAGG TATATATCGA CAACAGGATC GGCTTTAAAT TCCGGATTAA
      481 CGGCCTGTAC TAACAATACA AGACGTTCGA GGCCCATAGC AAAACCGACA GCCGGTGTTG
      541 CACGACCGCC CAGTTGTTCC ACAAGACCGT CATAACGACC GCCTGCACAC ACGGTGCCCT
      601 GGGAGCCGAG ACTGTTAGTC ACCCACTCGA AAACGGTACG GTTGTAGTAA TCCAGACCAC
      661 GCACCAGACG CTGGTTTACG GTGTAAGCGA TCCCCGCGCT CTCCAGCAGT TTGCACAGAC
      721 CGGCAAAATG CTCACGAGAT TCCTCGTCCA GATAGTCACC TAATGCCGGA GCGTCGTTGA
      781 GAAGCGCCTG CACTTCCGGA TTTTTTGAAT CCAGCACGCG CAGCGGGTTA GTGTACATGC
      841 GGCGTTTGCA GTCTTCGTCC AGCTTTTCTT TATGCTGCTC AAGGAATGCC ACCAGCGCAT
      901 CGCGGTAATT GGCGCGTGCT TCCAGCGAAC CGATAGAGTT CAGCTCAAGA GTTACGTGCT
      961 CGGAAATACC CAGCGCGCGC CACCAGCGGG CAGTGAGCAT AATCAGTTCA GCGTCGATAT
     1021 CCGGACCTTG CAGACCGAAA ACTTCGCAGC CCAACTGATG GAACTGACGA TAACGCCCTT
     1081 TCTGCGGACG CTCGTGACGG AACATCGGCC CGATATACCA CAGACGCTGT TCCTGATTGT
     1141 ACAGAAGACC ATGCTCGATG CCGGCGCGTA CACAGCCCGC CGTCCCTTCA GGGCGCAGAG
     1201 TCAGGCTGTC GCCATTGCGA TCCTCAAAGG TGTACATCTC TTTTTCAACC ACGTCGGTGA
     1261 CTTCACCAAT CGCACGTTTG AATAGCGGGG TCTGCTCTAC AATCGGCAAG CGGATTTCAC
     1321 TGTAACCGTA GCTGCCGAGC ACGTTTTTCA GTGTGCCTTC AATGCGCTGC CAGATGGCCG
     1381 TTTCGCCAGG CAGGTAATCG TTCATGCCGC GAATGGCTTG AATGTTTTTT GCCATATGTA
     1441 TATCTCCTTC TTAAAGTTAA ACAAAATTAT TTCTAGAGGG GAATTGTTAT CCGCTCACAA
     1501 TTCCCCTATA GTGAGTCGTA TTAATTTCGC GGGATCGAGA TCTCGATCCT CTACGCCGGA
     1561 CGCATCGTGG CCGGCATCAC CGGCGCCACA GGTGCGGTTG CTGGCGCCTA TATCGCCGAC
     1621 ATCACCGATG GGGAAGATCG GGCTCGCCAC TTCGGGCTCA TGAGCGCTTG TTTCGGCGTG
     1681 GGTATGGTGG CAGGCCCCGT GGCCGGGGGA CTGTTGGGCG CCATCTCCTT GCATGCACCA
     1741 TTCCTTGCGG CGGCGGTGCT CAACGGCCTC AACCTACTAC TGGGCTGCTT CCTAATGCAG
     1801 GAGTCGCATA AGGGAGAGCG TCGAGATCCC GGACACCATC GAATGGCGCA AAACCTTTCG
     1861 CGGTATGGCA TGATAGCGCC CGGAAGAGAG TCAATTCAGG GTGGTGAATG TGAAACCAGT
     1921 AACGTTATAC GATGTCGCAG AGTATGCCGG TGTCTCTTAT CAGACCGTTT CCCGCGTGGT
     1981 GAACCAGGCC AGCCACGTTT CTGCGAAAAC GCGGGAAAAA GTGGAAGCGG CGATGGCGGA
     2041 GCTGAATTAC ATTCCCAACC GCGTGGCACA ACAACTGGCG GGCAAACAGT CGTTGCTGAT
     2101 TGGCGTTGCC ACCTCCAGTC TGGCCCTGCA CGCGCCGTCG CAAATTGTCG CGGCGATTAA
     2161 ATCTCGCGCC GATCAACTGG GTGCCAGCGT GGTGGTGTCG ATGGTAGAAC GAAGCGGCGT
     2221 CGAAGCCTGT AAAGCGGCGG TGCACAATCT TCTCGCGCAA CGCGTCAGTG GGCTGATCAT
     2281 TAACTATCCG CTGGATGACC AGGATGCCAT TGCTGTGGAA GCTGCCTGCA CTAATGTTCC
     2341 GGCGTTATTT CTTGATGTCT CTGACCAGAC ACCCATCAAC AGTATTATTT TCTCCCATGA
     2401 AGACGGTACG CGACTGGGCG TGGAGCATCT GGTCGCATTG GGTCACCAGC AAATCGCGCT
     2461 GTTAGCGGGC CCATTAAGTT CTGTCTCGGC GCGTCTGCGT CTGGCTGGCT GGCATAAATA
     2521 TCTCACTCGC AATCAAATTC AGCCGATAGC GGAACGGGAA GGCGACTGGA GTGCCATGTC
     2581 CGGTTTTCAA CAAACCATGC AAATGCTGAA TGAGGGCATC GTTCCCACTG CGATGCTGGT
     2641 TGCCAACGAT CAGATGGCGC TGGGCGCAAT GCGCGCCATT ACCGAGTCCG GGCTGCGCGT
     2701 TGGTGCGGAT ATCTCGGTAG TGGGATACGA CGATACCGAA GACAGCTCAT GTTATATCCC
     2761 GCCGTTAACC ACCATCAAAC AGGATTTTCG CCTGCTGGGG CAAACCAGCG TGGACCGCTT
     2821 GCTGCAACTC TCTCAGGGCC AGGCGGTGAA GGGCAATCAG CTGTTGCCCG TCTCACTGGT
     2881 GAAAAGAAAA ACCACCCTGG CGCCCAATAC GCAAACCGCC TCTCCCCGCG CGTTGGCCGA
     2941 TTCATTAATG CAGCTGGCAC GACAGGTTTC CCGACTGGAA AGCGGGCAGT GAGCGCAACG
     3001 CAATTAATGT AAGTTAGCTC ACTCATTAGG CACCGGGATC TCGACCGATG CCCTTGAGAG
     3061 CCTTCAACCC AGTCAGCTCC TTCCGGTGGG CGCGGGGCAT GACTATCGTC GCCGCACTTA
     3121 TGACTGTCTT CTTTATCATG CAACTCGTAG GACAGGTGCC GGCAGCGCTC TGGGTCATTT
     3181 TCGGCGAGGA CCGCTTTCGC TGGAGCGCGA CGATGATCGG CCTGTCGCTT GCGGTATTCG
     3241 GAATCTTGCA CGCCCTCGCT CAAGCCTTCG TCACTGGTCC CGCCACCAAA CGTTTCGGCG
     3301 AGAAGCAGGC CATTATCGCC GGCATGGCGG CCCCACGGGT GCGCATGATC GTGCTCCTGT
     3361 CGTTGAGGAC CCGGCTAGGC TGGCGGGGTT GCCTTACTGG TTAGCAGAAT GAATCACCGA
     3421 TACGCGAGCG AACGTGAAGC GACTGCTGCT GCAAAACGTC TGCGACCTGA GCAACAACAT
     3481 GAATGGTCTT CGGTTTCCGT GTTTCGTAAA GTCTGGAAAC GCGGAAGTCA GCGCCCTGCA
     3541 CCATTATGTT CCGGATCTGC ATCGCAGGAT GCTGCTGGCT ACCCTGTGGA ACACCTACAT
     3601 CTGTATTAAC GAAGCGCTGG CATTGACCCT GAGTGATTTT TCTCTGGTCC CGCCGCATCC
     3661 ATACCGCCAG TTGTTTACCC TCACAACGTT CCAGTAACCG GGCATGTTCA TCATCAGTAA
     3721 CCCGTATCGT GAGCATCCTC TCTCGTTTCA TCGGTATCAT TACCCCCATG AACAGAAATC
     3781 CCCCTTACAC GGAGGCATCA GTGACCAAAC AGGAAAAAAC CGCCCTTAAC ATGGCCCGCT
     3841 TTATCAGAAG CCAGACATTA ACGCTTCTGG AGAAACTCAA CGAGCTGGAC GCGGATGAAC
     3901 AGGCAGACAT CTGTGAATCG CTTCACGACC ACGCTGATGA GCTTTACCGC AGCTGCCTCG
     3961 CGCGTTTCGG TGATGACGGT GAAAACCTCT GACACATGCA GCTCCCGGAG ACGGTCACAG
     4021 CTTGTCTGTA AGCGGATGCC GGGAGCAGAC AAGCCCGTCA GGGCGCGTCA GCGGGTGTTG
     4081 GCGGGTGTCG GGGCGCAGCC ATGACCCAGT CACGTAGCGA TAGCGGAGTG TATACTGGCT
     4141 TAACTATGCG GCATCAGAGC AGATTGTACT GAGAGTGCAC CATATATGCG GTGTGAAATA
     4201 CCGCACAGAT GCGTAAGGAG AAAATACCGC ATCAGGCGCT CTTCCGCTTC CTCGCTCACT
     4261 GACTCGCTGC GCTCGGTCGT TCGGCTGCGG CGAGCGGTAT CAGCTCACTC AAAGGCGGTA
     4321 ATACGGTTAT CCACAGAATC AGGGGATAAC GCAGGAAAGA ACATGTGAGC AAAAGGCCAG
     4381 CAAAAGGCCA GGAACCGTAA AAAGGCCGCG TTGCTGGCGT TTTTCCATAG GCTCCGCCCC
     4441 CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA
     4501 TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG
     4561 CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC
     4621 TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC
     4681 GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC
     4741 CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG
     4801 AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA
     4861 AGGACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT
     4921 AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG
     4981 CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT TGATCTTTTC TACGGGGTCT
     5041 GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGAGATT ATCAAAAAGG
     5101 ATCTTCACCT AGATCCTTTT AAATTAAAAA TGAAGTTTTA AATCAATCTA AAGTATATAT
     5161 GAGTAAACTT GGTCTGACAG TTACCAATGC TTAATCAGTG AGGCACCTAT CTCAGCGATC
     5221 TGTCTATTTC GTTCATCCAT AGTTGCCTGA CTCCCCGTCG TGTAGATAAC TACGATACGG
     5281 GAGGGCTTAC CATCTGGCCC CAGTGCTGCA ATGATACCGC GAGACCCACG CTCACCGGCT
     5341 CCAGATTTAT CAGCAATAAA CCAGCCAGCC GGAAGGGCCG AGCGCAGAAG TGGTCCTGCA
     5401 ACTTTATCCG CCTCCATCCA GTCTATTAAT TGTTGCCGGG AAGCTAGAGT AAGTAGTTCG
     5461 CCAGTTAATA GTTTGCGCAA CGTTGTTGCC ATTGCTGCAG GCATCGTGGT GTCACGCTCG
     5521 TCGTTTGGTA TGGCTTCATT CAGCTCCGGT TCCCAACGAT CAAGGCGAGT TACATGATCC
     5581 CCCATGTTGT GCAAAAAAGC GGTTAGCTCC TTCGGTCCTC CGATCGTTGT CAGAAGTAAG
     5641 TTGGCCGCAG TGTTATCACT CATGGTTATG GCAGCACTGC ATAATTCTCT TACTGTCATG
     5701 CCATCCGTAA GATGCTTTTC TGTGACTGGT GAGTACTCAA CCAAGTCATT CTGAGAATAG
     5761 TGTATGCGGC GACCGAGTTG CTCTTGCCCG GCGTCAATAC GGGATAATAC CGCGCCACAT
     5821 AGCAGAACTT TAAAAGTGCT CATCATTGGA AAACGTTCTT CGGGGCGAAA ACTCTCAAGG
     5881 ATCTTACCGC TGTTGAGATC CAGTTCGATG TAACCCACTC GTGCACCCAA CTGATCTTCA
     5941 GCATCTTTTA CTTTCACCAG CGTTTCTGGG TGAGCAAAAA CAGGAAGGCA AAATGCCGCA
     6001 AAAAAGGGAA TAAGGGCGAC ACGGAAATGT TGAATACTCA TACTCTTCCT TTTTCAATAT
     6061 TATTGAAGCA TTTATCAGGG TTATTGTCTC ATGAGCGGAT ACATATTTGA ATGTATTTAG
     6121 AAAAATAAAC AAATAGGGGT TCCGCGCACA TTTCCCCGAA AAGTGCCACC TGAAATTGTA
     6181 AACGTTAATA TTTTGTTAAA ATTCGCGTTA AATTTTTGTT AAATCAGCTC ATTTTTTAAC
     6241 CAATAGGCCG AAATCGGCAA AATCCCTTAT AAATCAAAAG AATAGACCGA GATAGGGTTG
     6301 AGTGTTGTTC CAGTTTGGAA CAAGAGTCCA CTATTAAAGA ACGTGGACTC CAACGTCAAA
     6361 GGGCGAAAAA CCGTCTATCA GGGCGATGGC CCACTACGTG AACCATCACC CTAATCAAGT
     6421 TTTTTGGGGT CGAGGTGCCG TAAAGCACTA AATCGGAACC CTAAAGGGAG CCCCCGATTT
     6481 AGAGCTTGAC GGGGAAAGCC GGCGAACGTG GCGAGAAAGG AAGGGAAGAA AGCGAAAGGA
     6541 GCGGGCGCTA GGGCGCTGGC AAGTGTAGCG GTCACGCTGC GCGTAACCAC CACACCCGCC
     6601 GCGCTTAATG CGCCGCTACA GGGCGCGTCC CATTCGCCA
//