LOCUS       Exported                8217 bp ds-DNA     circular SYN 19-FEB-2019
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8217)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Feb 19, 2019 from SnapGene Viewer 4.3.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8217
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     terminator      26..73
                     /label=T7 terminator
     CDS             complement(140..157)
                     /codon_start=1
                     /label=6x His Tag
                     /translation="HHHHHH"
     misc_feature    158..173
                     /label=MCS
     misc_feature    complement(179..3013)
                     /label=IleRS
     CDS             complement(2993..3010)
                     /codon_start=1
                     /label=6x His Tag
                     /translation="HHHHHH"
     RBS             3022..3027
                     /label=RBS
     protein_bind    3058..3082
                     /label=lac operator
     misc_binding    complement(3060..3082)
                     /label=LacO
     primer_bind     complement(3082..3101)
                     /label=T7
     promoter        complement(3083..3101)
                     /label=T7 promoter
     promoter        3410..3487
                     /label=lacI promoter
     CDS             3488..4570
                     /codon_start=1
                     /label=lacI
                     /translation="VKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     CDS             5379..5570
                     /codon_start=1
                     /label=rop
                     /translation="VTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     rep_origin      complement(5982..6664)
                     /direction=LEFT
                     /label=ColE1 origin
     CDS             complement(6759..7550)
                     /codon_start=1
                     /label=AmpR
                     /translation="HPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMS
                     TFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITM
                     SDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPAAMAT
                     TLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIA
                     ALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW"
     CDS             complement(7551..7619)
                     /codon_start=1
                     /label=AmpR
                     /translation="MSIQHFRVALIPFFAAFCLPVFA"
     promoter        complement(7620..7724)
                     /label=AmpR promoter
     rep_origin      complement(7751..8206)
                     /direction=LEFT
                     /label=f1 ori
ORIGIN
        1 atccggatat agttcctcct ttcagcaaaa aacccctcaa gacccgttta gaggccccaa
       61 ggggttatgc tagttattgc tcagcggtgg cagcagccaa ctcagcttcc tttcgggctt
      121 tgttagcagc cggatctcag tggtggtggt ggtggtgctc gagtgcggcc gcaagctttc
      181 aggcaaactt acgtttttca ccgtcaccgg cgacgttgct gacacagcgg ccgcagattt
      241 ctgcgtgttc cgccaccttg ccgacatcct gggtgtagtg ccagcagcgt gggcacttct
      301 caccttcggc tttactcaac gcgactttca gccctttgag tacttcgctc tgctgagcat
      361 cagcaggtgc gtcgttatag tctgcaacgg tagcgccgga ggtcaacagg acaaatcgta
      421 attcatcgcc cagcgcggtc agtttcgccg acagttccgg ttctgcatac aaggttactg
      481 ccgcttccag cgagccaccc actttcttgt cggcacgcgc ttgctcaatg actttgttca
      541 cttcgccacg cactttcaac agctcgtccc agaacgcatc gttcatcgct tcactgtctg
      601 ccaggccaaa caggccttcg taccactcac cggtgaagac gtatttttca cgttcgcccg
      661 gcaggtagcc ccacacttca tcagcggtga aggagaggat tggtgccatc cagcgcacca
      721 gcgcttctgc gatgtgatat agcgcagtct ggcagctacg acgcgccaca ctgtccgctt
      781 tggcggtgta ctgacggtct ttgatgatgt cgaggtagaa ggaacccatc tcaacggagc
      841 agaagcgcat cagacgctgt accacttcgt ggaaatcgta tgcttcgtac gccttgagga
      901 tgtcttcctg tgccgctttc gcacaaccta cggcccagcg atccagtacc accatctctt
      961 ccggtttcac catatctttt gctggatcaa aaccgttcag gtttgccagc aggaagcgcg
     1021 cggtgttacg gatacgacga tagctatcgg cagcacgttt caggatctcg tcagaaacgg
     1081 ccatttcacc ggtgtagtcg gttgatgcca cccacagacg cagaatatcc gcgcccagtt
     1141 tgttcatcac atcctgcggc gaaacggtat tgccgatgga tttagacatc ttgcggccct
     1201 gaccatccac ggtaaagccg tgggtcagta cctgacgata cggcgcttta cccttcatcg
     1261 cggtggagat cattagggaa gacatgaacc agccgcggtg ttggtcagaa ccttccagat
     1321 acatgtccgc tgcgtgaccg gcaaattccg gacgcacgtc aacaacagaa gagtgggtag
     1381 atccggagtc aaaccataca tccaatgtgt ccggcacttt cacgtactga tcagcttcgt
     1441 cgccgaggat ctctttcgca tcgagatccc accacgcctg gatgccatcg acttcaacgc
     1501 gttttgccac ttcttccatc agttcaaggg tacgcggatg cagctcttcc gtgtctttgt
     1561 gcacgaacag tgacatcggt acaccccagg tgcgctgacg ggagatacac cagtcaggac
     1621 ggttagcaac catcgactcg atacgcgcct ggccccagtc cgggatccac tgcacgcctt
     1681 tgatctcttt cagtgactgc gcacgcagac ctttctgatc catgctgacg aaccactgcg
     1741 gcgtcgcgcg gaagatgatc ggcgttttgt gacgccagca gcacggatag ctgtgctgca
     1801 ttttctcaac gtgcagcagc gcgccttttt cctgcagcag cgcaacgacg atgtcgttcg
     1861 ctttgaagac gttcacgcca tccagcgtcg gataagtgcc cggcagataa gtgccgtccg
     1921 ggccaaccgg gttagcggtt tccaggccgt atttctgacc gatcacatag tcgtccgggc
     1981 cgtggccagg cgcggtgtga acggcaccgg taccggcatc cagggtaacg tgatcgccga
     2041 ggattgccgg aacgtcgaag cccataaacg gatgggtaaa gcgcagcagc tcaagctccg
     2101 caccttttac cgtgccgaga atggtgtaat cggtcacgcc gatacgctgc attacgcttt
     2161 caaccagatc tttcgccaga atcacggcct gaccgtcgat ctgcaccagc gcatagtcga
     2221 aatctggtgc aatagagatt gcgcggttgg caggcagagt ccacggcgtg gtggtccaga
     2281 ttaccagcga gattgggccg ttaacgttgc ttacggcaaa ttttgctttc agtgcatcct
     2341 gatcgactgc ctggaaagca acgtcgatgg acggagaagt tttgtcgtaa tactcaactt
     2401 ccgcttccgc cagcgcagaa cggcagtcaa cgcaccagtg aactggcttc gcgcctttgt
     2461 gcaggtgacc gttgccgatg attttgccca gcgcgcggat gatgttggct tcagttttga
     2521 agtccatggt caggtacggg tgcgaccagt cgcccagcac gcccagacgg ataaagtctt
     2581 tgcgttgacc gtcaacctgg gtcgccgcgt attcgcggca cttggcgcgg aactcggcgg
     2641 cggtgaattt ctcacccggc ttaccgtatt cttgctcgac tttcagctcg atcggcagac
     2701 cgtggcagtc ccagccaggc acatacggcg agtcataacc ggaaagccct ttggacttca
     2761 cgataatgtc tttcagaatc ttgttaaccg agtgaccaat atgaatgctg ccattcgcat
     2821 aaggagggcc atcatgcaga atgaaggttt ttttgccttt tttagccgca cggatgatgc
     2881 cgtacagatc atcatcagtc caacgcgcca gcattccggg ttcgcgcttg gcgagatcgc
     2941 cacgcatcgg gaaccctgtt tccggcaaat tcagggttga tttatagtca ctgtggtggt
     3001 ggtggtggtg catatgtata tctccttctt aaagttaaac aaaattattt ctagagggga
     3061 attgttatcc gctcacaatt cccctatagt gagtcgtatt aatttcgcgg gatcgagatc
     3121 tcgatcctct acgccggacg catcgtggcc ggcatcaccg gcgccacagg tgcggttgct
     3181 ggcgcctata tcgccgacat caccgatggg gaagatcggg ctcgccactt cgggctcatg
     3241 agcgcttgtt tcggcgtggg tatggtggca ggccccgtgg ccgggggact gttgggcgcc
     3301 atctccttgc atgcaccatt ccttgcggcg gcggtgctca acggcctcaa cctactactg
     3361 ggctgcttcc taatgcagga gtcgcataag ggagagcgtc gagatcccgg acaccatcga
     3421 atggcgcaaa acctttcgcg gtatggcatg atagcgcccg gaagagagtc aattcagggt
     3481 ggtgaatgtg aaaccagtaa cgttatacga tgtcgcagag tatgccggtg tctcttatca
     3541 gaccgtttcc cgcgtggtga accaggccag ccacgtttct gcgaaaacgc gggaaaaagt
     3601 ggaagcggcg atggcggagc tgaattacat tcccaaccgc gtggcacaac aactggcggg
     3661 caaacagtcg ttgctgattg gcgttgccac ctccagtctg gccctgcacg cgccgtcgca
     3721 aattgtcgcg gcgattaaat ctcgcgccga tcaactgggt gccagcgtgg tggtgtcgat
     3781 ggtagaacga agcggcgtcg aagcctgtaa agcggcggtg cacaatcttc tcgcgcaacg
     3841 cgtcagtggg ctgatcatta actatccgct ggatgaccag gatgccattg ctgtggaagc
     3901 tgcctgcact aatgttccgg cgttatttct tgatgtctct gaccagacac ccatcaacag
     3961 tattattttc tcccatgaag acggtacgcg actgggcgtg gagcatctgg tcgcattggg
     4021 tcaccagcaa atcgcgctgt tagcgggccc attaagttct gtctcggcgc gtctgcgtct
     4081 ggctggctgg cataaatatc tcactcgcaa tcaaattcag ccgatagcgg aacgggaagg
     4141 cgactggagt gccatgtccg gttttcaaca aaccatgcaa atgctgaatg agggcatcgt
     4201 tcccactgcg atgctggttg ccaacgatca gatggcgctg ggcgcaatgc gcgccattac
     4261 cgagtccggg ctgcgcgttg gtgcggatat ctcggtagtg ggatacgacg ataccgaaga
     4321 cagctcatgt tatatcccgc cgttaaccac catcaaacag gattttcgcc tgctggggca
     4381 aaccagcgtg gaccgcttgc tgcaactctc tcagggccag gcggtgaagg gcaatcagct
     4441 gttgcccgtc tcactggtga aaagaaaaac caccctggcg cccaatacgc aaaccgcctc
     4501 tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag
     4561 cgggcagtga gcgcaacgca attaatgtaa gttagctcac tcattaggca ccgggatctc
     4621 gaccgatgcc cttgagagcc ttcaacccag tcagctcctt ccggtgggcg cggggcatga
     4681 ctatcgtcgc cgcacttatg actgtcttct ttatcatgca actcgtagga caggtgccgg
     4741 cagcgctctg ggtcattttc ggcgaggacc gctttcgctg gagcgcgacg atgatcggcc
     4801 tgtcgcttgc ggtattcgga atcttgcacg ccctcgctca agccttcgtc actggtcccg
     4861 ccaccaaacg tttcggcgag aagcaggcca ttatcgccgg catggcggcc ccacgggtgc
     4921 gcatgatcgt gctcctgtcg ttgaggaccc ggctaggctg gcggggttgc cttactggtt
     4981 agcagaatga atcaccgata cgcgagcgaa cgtgaagcga ctgctgctgc aaaacgtctg
     5041 cgacctgagc aacaacatga atggtcttcg gtttccgtgt ttcgtaaagt ctggaaacgc
     5101 ggaagtcagc gccctgcacc attatgttcc ggatctgcat cgcaggatgc tgctggctac
     5161 cctgtggaac acctacatct gtattaacga agcgctggca ttgaccctga gtgatttttc
     5221 tctggtcccg ccgcatccat accgccagtt gtttaccctc acaacgttcc agtaaccggg
     5281 catgttcatc atcagtaacc cgtatcgtga gcatcctctc tcgtttcatc ggtatcatta
     5341 cccccatgaa cagaaatccc ccttacacgg aggcatcagt gaccaaacag gaaaaaaccg
     5401 cccttaacat ggcccgcttt atcagaagcc agacattaac gcttctggag aaactcaacg
     5461 agctggacgc ggatgaacag gcagacatct gtgaatcgct tcacgaccac gctgatgagc
     5521 tttaccgcag ctgcctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc
     5581 tcccggagac ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg
     5641 gcgcgtcagc gggtgttggc gggtgtcggg gcgcagccat gacccagtca cgtagcgata
     5701 gcggagtgta tactggctta actatgcggc atcagagcag attgtactga gagtgcacca
     5761 tatatgcggt gtgaaatacc gcacagatgc gtaaggagaa aataccgcat caggcgctct
     5821 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
     5881 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
     5941 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
     6001 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
     6061 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
     6121 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
     6181 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
     6241 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
     6301 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
     6361 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
     6421 aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc
     6481 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
     6541 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
     6601 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
     6661 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
     6721 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag
     6781 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg
     6841 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga
     6901 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag
     6961 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa
     7021 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctgcaggc
     7081 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca
     7141 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg
     7201 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat
     7261 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc
     7321 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg
     7381 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg
     7441 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt
     7501 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca
     7561 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata
     7621 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac
     7681 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa
     7741 gtgccacctg aaattgtaaa cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa
     7801 atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa
     7861 tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac
     7921 gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa
     7981 ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct
     8041 aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa
     8101 gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc
     8161 gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtccca ttcgcca
//
