LOCUS       Exported                7924 bp ds-DNA     circular SYN 19-FEB-2019
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7924)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Feb 19, 2019 from SnapGene Viewer 4.3.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7924
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     terminator      26..73
                     /label=T7 terminator
     CDS             complement(140..157)
                     /codon_start=1
                     /label=6x His Tag
                     /translation="HHHHHH"
     misc_feature    158
                     /label=MCS
     misc_feature    complement(159..2743)
                     /label=LeuRS
     protein_bind    2765..2789
                     /label=lac operator
     misc_binding    complement(2767..2789)
                     /label=LacO
     primer_bind     complement(2789..2808)
                     /label=T7
     promoter        complement(2790..2808)
                     /label=T7 promoter
     promoter        3117..3194
                     /label=lacI promoter
     CDS             3195..4277
                     /codon_start=1
                     /label=lacI
                     /translation="VKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     CDS             5086..5277
                     /codon_start=1
                     /label=rop
                     /translation="VTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     rep_origin      complement(5689..6371)
                     /direction=LEFT
                     /label=ColE1 origin
     CDS             complement(6466..7257)
                     /codon_start=1
                     /label=AmpR
                     /translation="HPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMS
                     TFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITM
                     SDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPAAMAT
                     TLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIA
                     ALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW"
     CDS             complement(7258..7326)
                     /codon_start=1
                     /label=AmpR
                     /translation="MSIQHFRVALIPFFAAFCLPVFA"
     promoter        complement(7327..7431)
                     /label=AmpR promoter
     rep_origin      complement(7458..7913)
                     /direction=LEFT
                     /label=f1 ori
ORIGIN
        1 atccggatat agttcctcct ttcagcaaaa aacccctcaa gacccgttta gaggccccaa
       61 ggggttatgc tagttattgc tcagcggtgg cagcagccaa ctcagcttcc tttcgggctt
      121 tgttagcagc cggatctcag tggtggtggt ggtggtgctc gaggccaacg accagattga
      181 ggagtttacc tggtacgtaa atcactttac gtacagtaac gccatcaaga tattttgcta
      241 ccagatgttc ctggccagca cgttcgcgaa cctgttcttc cgttgcgtcc accggaacgg
      301 tgattttggc acggacttta ccgttaacct gcaccacgac cagcgtggag tcttccacca
      361 tcgctttttc gtcagcaacc ggccacggcg cgttgtcgat atcgccttcg cctttcagtt
      421 cctgccacag cgtgaagcag atgtgcgggg tgaacgggtt aagcatacgg acaacggcca
      481 gcagtgcttc ctgcatcaga gcgcgatcct gctcgccatc ggttggtgct ttcgccagtt
      541 tgttcatcag ctccataatc gccgcaattg cggtgttgaa ggtctgacga cggccgatat
      601 catcggtcac tttagcgatc gttttatgca catcgcgacg cagcgctttc tgattttcag
      661 tcagcgcatc aacgttcagt gccgcaacat caccttttgc tgtgtgctcg taaaccagtt
      721 tccagacacg tttcaggaag cggttagccc cttccacacc ggattcctgc cattcgagag
      781 tcatatcagc cggagaagca aacatcataa acagacgaac ggtgtccgcg ccgtaacgtt
      841 caaccatcac ctgcgggtcg ataccgttgt tcttcgactt ggacattttg ctcatgccgg
      901 tataaaccag ttcatggcct gccgcatctt tcgctttcac gatacggcct ttctcgtcac
      961 gttcaacgat agcatcaacc ggggaaaccc agttacgttc gccgttttcg ccaacatagt
     1021 agaaggcatc tgccagcacc ataccctgac acagcaactg tttcgctggt tcgtcagagt
     1081 tcaccatgcc tgcatcacgc atcagtttgt ggaagaagcg gaagtagagc aggtgcataa
     1141 tggcgtgttc aataccacca atgtagatat ccaccggcag ccagtagtta gccgcttcgg
     1201 aatccagcat accttctttg tactgcgggc aagtgtagcg cgcatagtac caggaggact
     1261 ccataaaggt gtcgaaagtg tcggtttcac gcagtgctgg cataccgtta acggtagttt
     1321 tcgcccactc cggatctgct ttaatcgggc tggtaatgcc gtccattacc acatcttccg
     1381 gcaggatcac cggcagctgg tcgtccgggg tcggcattac ggtaccgtct tccagcgtca
     1441 ccatcggaat cggcgcgccc cagtaacgct gacgggaaac accccagtcg cgcaggcggt
     1501 agttcacttt acgctcgcca acgcccatcg cagtcagttt atcggcgatg gcgttgaagg
     1561 ccgcttcatg gtcaagaccg ttgaactcgc cagagttgaa cagcacgcct ttttcagtca
     1621 gggcttgctg agaaagatct ggctcagagc cgtcagctgc caggataacc ggtttgatgt
     1681 tcaggccgta tttagaggca aactcgtagt cgcgctggtc gtgccccggt accgccataa
     1741 ctgcgcccgt gccgtactcc atcaatacga agtttgctgc ccaaacggga atttcttcgc
     1801 ccgttaatgg gtgaaccgct ttaaagccag tatcgacgcc ttttttctcc atcgtcgcca
     1861 tttcagcttc ggcaactttg gtgttacggc attcgtcaat aaaggccgcc agttcaggat
     1921 tattttccgc cgctttctgc gccagcggat gacccgcagc taccgccagg taggtacaac
     1981 ccataaaggt gtccgggcgg gtagtgtaaa cggtcagcgt gttgtcatag tcgttaacgt
     2041 tgaaggtgat ctccacgcct tcggaacgac cgatccagtt acgctgcatg gttttaacgg
     2101 tgtctggcca gtgatccagt ttatccagat cgttgagcag ctcgtcagcg taagcagtga
     2161 ttttgataaa ccactgcggg atctctttac gttcaacttt ggtatcgcag cgccagcagc
     2221 agccgtcgat aacttgttcg ttcgccagta cggtctggtc gttcgggcac cagttgaccg
     2281 cagaagtctt cttatatacc aggccttttt tatacagctc ggtgaagaat ttctgttccc
     2341 aacggtagta ttccggcgta caggttgcca gctcgcggct ccagtcataa ccaaagccca
     2401 gcattttgag ctggtttttc atatacgcga tgttgtcgta cgtccacggt gccggagcgg
     2461 tgttgttttt caccgccgcg ccttccgcag gcagaccaaa cgcgtcccag ccgatcggct
     2521 gcaggacgtt tttgcccagc atacgctggt agcgggcgat cacgtcaccg atggtgtagt
     2581 tacgtacgtg gcccatgtgt agtcgaccag aaggataggg aagcatagac aggcagtaat
     2641 acttctcttt gctctcgtct tcggttactt caaatgtgcg cttctcatcc caatgaagct
     2701 gtactttgga ttctatctct tccgggcggt attgctcttg catggcagca tctccttcta
     2761 gaggggaatt gttatccgct cacaattccc ctatagtgag tcgtattaat ttcgcgggat
     2821 cgagatctcg atcctctacg ccggacgcat cgtggccggc atcaccggcg ccacaggtgc
     2881 ggttgctggc gcctatatcg ccgacatcac cgatggggaa gatcgggctc gccacttcgg
     2941 gctcatgagc gcttgtttcg gcgtgggtat ggtggcaggc cccgtggccg ggggactgtt
     3001 gggcgccatc tccttgcatg caccattcct tgcggcggcg gtgctcaacg gcctcaacct
     3061 actactgggc tgcttcctaa tgcaggagtc gcataaggga gagcgtcgag atcccggaca
     3121 ccatcgaatg gcgcaaaacc tttcgcggta tggcatgata gcgcccggaa gagagtcaat
     3181 tcagggtggt gaatgtgaaa ccagtaacgt tatacgatgt cgcagagtat gccggtgtct
     3241 cttatcagac cgtttcccgc gtggtgaacc aggccagcca cgtttctgcg aaaacgcggg
     3301 aaaaagtgga agcggcgatg gcggagctga attacattcc caaccgcgtg gcacaacaac
     3361 tggcgggcaa acagtcgttg ctgattggcg ttgccacctc cagtctggcc ctgcacgcgc
     3421 cgtcgcaaat tgtcgcggcg attaaatctc gcgccgatca actgggtgcc agcgtggtgg
     3481 tgtcgatggt agaacgaagc ggcgtcgaag cctgtaaagc ggcggtgcac aatcttctcg
     3541 cgcaacgcgt cagtgggctg atcattaact atccgctgga tgaccaggat gccattgctg
     3601 tggaagctgc ctgcactaat gttccggcgt tatttcttga tgtctctgac cagacaccca
     3661 tcaacagtat tattttctcc catgaagacg gtacgcgact gggcgtggag catctggtcg
     3721 cattgggtca ccagcaaatc gcgctgttag cgggcccatt aagttctgtc tcggcgcgtc
     3781 tgcgtctggc tggctggcat aaatatctca ctcgcaatca aattcagccg atagcggaac
     3841 gggaaggcga ctggagtgcc atgtccggtt ttcaacaaac catgcaaatg ctgaatgagg
     3901 gcatcgttcc cactgcgatg ctggttgcca acgatcagat ggcgctgggc gcaatgcgcg
     3961 ccattaccga gtccgggctg cgcgttggtg cggatatctc ggtagtggga tacgacgata
     4021 ccgaagacag ctcatgttat atcccgccgt taaccaccat caaacaggat tttcgcctgc
     4081 tggggcaaac cagcgtggac cgcttgctgc aactctctca gggccaggcg gtgaagggca
     4141 atcagctgtt gcccgtctca ctggtgaaaa gaaaaaccac cctggcgccc aatacgcaaa
     4201 ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac
     4261 tggaaagcgg gcagtgagcg caacgcaatt aatgtaagtt agctcactca ttaggcaccg
     4321 ggatctcgac cgatgccctt gagagccttc aacccagtca gctccttccg gtgggcgcgg
     4381 ggcatgacta tcgtcgccgc acttatgact gtcttcttta tcatgcaact cgtaggacag
     4441 gtgccggcag cgctctgggt cattttcggc gaggaccgct ttcgctggag cgcgacgatg
     4501 atcggcctgt cgcttgcggt attcggaatc ttgcacgccc tcgctcaagc cttcgtcact
     4561 ggtcccgcca ccaaacgttt cggcgagaag caggccatta tcgccggcat ggcggcccca
     4621 cgggtgcgca tgatcgtgct cctgtcgttg aggacccggc taggctggcg gggttgcctt
     4681 actggttagc agaatgaatc accgatacgc gagcgaacgt gaagcgactg ctgctgcaaa
     4741 acgtctgcga cctgagcaac aacatgaatg gtcttcggtt tccgtgtttc gtaaagtctg
     4801 gaaacgcgga agtcagcgcc ctgcaccatt atgttccgga tctgcatcgc aggatgctgc
     4861 tggctaccct gtggaacacc tacatctgta ttaacgaagc gctggcattg accctgagtg
     4921 atttttctct ggtcccgccg catccatacc gccagttgtt taccctcaca acgttccagt
     4981 aaccgggcat gttcatcatc agtaacccgt atcgtgagca tcctctctcg tttcatcggt
     5041 atcattaccc ccatgaacag aaatccccct tacacggagg catcagtgac caaacaggaa
     5101 aaaaccgccc ttaacatggc ccgctttatc agaagccaga cattaacgct tctggagaaa
     5161 ctcaacgagc tggacgcgga tgaacaggca gacatctgtg aatcgcttca cgaccacgct
     5221 gatgagcttt accgcagctg cctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac
     5281 atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag cagacaagcc
     5341 cgtcagggcg cgtcagcggg tgttggcggg tgtcggggcg cagccatgac ccagtcacgt
     5401 agcgatagcg gagtgtatac tggcttaact atgcggcatc agagcagatt gtactgagag
     5461 tgcaccatat atgcggtgtg aaataccgca cagatgcgta aggagaaaat accgcatcag
     5521 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
     5581 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
     5641 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
     5701 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
     5761 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
     5821 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
     5881 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
     5941 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
     6001 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
     6061 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
     6121 gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc
     6181 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
     6241 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
     6301 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
     6361 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
     6421 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
     6481 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
     6541 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
     6601 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
     6661 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
     6721 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
     6781 tgcaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
     6841 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
     6901 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
     6961 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
     7021 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
     7081 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
     7141 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
     7201 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
     7261 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
     7321 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
     7381 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
     7441 ccgaaaagtg ccacctgaaa ttgtaaacgt taatattttg ttaaaattcg cgttaaattt
     7501 ttgttaaatc agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc
     7561 aaaagaatag accgagatag ggttgagtgt tgttccagtt tggaacaaga gtccactatt
     7621 aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg atggcccact
     7681 acgtgaacca tcaccctaat caagtttttt ggggtcgagg tgccgtaaag cactaaatcg
     7741 gaaccctaaa gggagccccc gatttagagc ttgacgggga aagccggcga acgtggcgag
     7801 aaaggaaggg aagaaagcga aaggagcggg cgctagggcg ctggcaagtg tagcggtcac
     7861 gctgcgcgta accaccacac ccgccgcgct taatgcgccg ctacagggcg cgtcccattc
     7921 gcca
//
