LOCUS       Exported                6882 bp ds-DNA     circular SYN 19-FEB-2019
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6882)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Feb 19, 2019 from SnapGene Viewer 4.3.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6882
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     terminator      26..73
                     /label=T7 terminator
     CDS             complement(137..1678)
                     /codon_start=1
                     /label=LysRS
                     /translation="MSEQETRGANEAIDFNDELRNRREKLAALRQQGVAFPNDFRRDHT
                     SDQLHEEFDAKDNQELESLNIEVSVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARDSL
                     PEGVYNDQFKKWDLGDIIGARGTLFKTQTGELSIHCTELRLLTKALRPLPDKFHGLQDQ
                     EVRYRQRYLDLIANDKSRQTFVVRSKILAAIRQFMVARGFMEVETPMMQVIPGGASARP
                     FITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFTMMELY
                     MAYADYHDLIELTESLFRTLAQEVLGTTKVTYGEHVFDFGKPFEKLTMREAIKKYRPET
                     DMADLDNFDAAKALAESIGITVEKSWGLGRIVTEIFDEVAEAHLIQPTFITEYPAEVSP
                     LARRNDVNPEITDRFEFFIGGREIGNGFSELNDAEDQAERFQEQVNAKAAGDDEAMFYD
                     EDYVTALEYGLPPTAGLGIGIDRMIMLFTNSHTIRDVILFPAMRPQKLEHHHHHH"
     CDS             complement(140..157)
                     /codon_start=1
                     /label=6xHis
                     /translation="HHHHHH"
     misc_feature    158
                     /label=MCS
     RBS             1687..1692
                     /label=RBS
     protein_bind    1723..1747
                     /label=lac operator
     promoter        complement(1748..1766)
                     /label=T7 promoter
     promoter        2075..2152
                     /label=lacI promoter
     CDS             2153..3235
                     /codon_start=1
                     /label=lacI
                     /translation="VKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     CDS             4044..4235
                     /codon_start=1
                     /label=rop
                     /translation="VTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     rep_origin      complement(4665..5253)
                     /direction=LEFT
                     /label=ori
     CDS             complement(5424..6215)
                     /codon_start=1
                     /label=AmpR
                     /translation="HPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMS
                     TFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITM
                     SDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPAAMAT
                     TLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIA
                     ALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW"
     CDS             complement(6216..6284)
                     /codon_start=1
                     /label=AmpR
                     /translation="MSIQHFRVALIPFFAAFCLPVFA"
     promoter        complement(6285..6389)
                     /label=AmpR promoter
     rep_origin      complement(6416..6871)
                     /direction=LEFT
                     /label=f1 ori
ORIGIN
        1 atccggatat agttcctcct ttcagcaaaa aacccctcaa gacccgttta gaggccccaa
       61 ggggttatgc tagttattgc tcagcggtgg cagcagccaa ctcagcttcc tttcgggctt
      121 tgttagcagc cggatctcag tggtggtggt ggtggtgctc gagtttctgt gggcgcatcg
      181 ccgggaagag aataacgtcg cgaatagtat ggctgttagt aaacagcata atcattcggt
      241 cgataccaat acccagacca gcggttggcg gcagaccata ttccagcgca gtcacgtaat
      301 cttcgtcata gaacatggct tcgtcgtcac ctgcagcttt agcattaacc tgttcctgga
      361 aacgttcagc ctgatcttct gcgtcgttta attcgctaaa accattaccg atttcacgac
      421 caccgatgaa gaattcaaaa cggtcggtga tttccgggtt aacatcatta cggcgtgcca
      481 gcggggacac ttctgccgga tattccgtaa taaaggttgg ctgaatcaga tgtgcttctg
      541 ccacttcatc aaagatctct gtgacaatac gtcccaaccc ccagcttttc tctaccgtaa
      601 taccgataga ttcagctaat gctttagcag catcaaaatt atccaggtcg gccatatcgg
      661 tttctggacg atattttttg attgcttcgc gcatggtgag tttttcaaac ggtttgccga
      721 aatcaaacac atgctcgcca taagtgactt tagtggtacc cagaacctct tgtgccaggg
      781 tgcggaacag tgactctgtc agttcaatca aatcgtggta atccgcatac gccatgtaga
      841 gttccatcat tgtgaactca ggattatggc gaacagaaat accttcatta cggaagttac
      901 ggttgatttc gaatacccgt tcaaaaccgc ctacaaccag acgtttcaga tacagctccg
      961 gcgcgatacg cagatacata tctaaatcca gagcattatg atgggtaata aacgggcgag
     1021 cagatgcccc acctggaatt acctgcatca tcggggtttc tacttccata aagccgcgcg
     1081 cgaccatgaa ttgacggata gcggccagaa tttttgaacg gacaacaaac gtttgacggg
     1141 atttatcgtt agcgatgagg tccagataac gttgacgata acggacttcc tgatcctgca
     1201 gaccatggaa tttatctggt aaaggacgta gtgctttagt cagcaggcgc agctcagtac
     1261 agtgaatgga aagctcaccc gtttgcgtct taaacagcgt accgcgggca ccgataatgt
     1321 cacccagatc ccatttttta aactgatcgt tataaacacc ttctggcagg ctatctcttg
     1381 caacgtacag ttgaatacgg ccaccgacat cctgcaacgt tacaaaggag gctttcccca
     1441 tgatacgacg ggtcatcatt cggccagcaa ccgagacttc aatgtttaag gattccagtt
     1501 cctggttatc cttcgcatca aactcttcgt gcaactggtc agaggtatgg tcgcggcgaa
     1561 aatcattggg aaacgccaca ccttgctgac gtagtgccgc cagtttttcg cggcgatttc
     1621 tcagttcatc gttaaaatca atagcctcat tggctccccg tgtttcttgt tcagacatat
     1681 gtatatctcc ttcttaaagt taaacaaaat tatttctaga ggggaattgt tatccgctca
     1741 caattcccct atagtgagtc gtattaattt cgcgggatcg agatctcgat cctctacgcc
     1801 ggacgcatcg tggccggcat caccggcgcc acaggtgcgg ttgctggcgc ctatatcgcc
     1861 gacatcaccg atggggaaga tcgggctcgc cacttcgggc tcatgagcgc ttgtttcggc
     1921 gtgggtatgg tggcaggccc cgtggccggg ggactgttgg gcgccatctc cttgcatgca
     1981 ccattccttg cggcggcggt gctcaacggc ctcaacctac tactgggctg cttcctaatg
     2041 caggagtcgc ataagggaga gcgtcgagat cccggacacc atcgaatggc gcaaaacctt
     2101 tcgcggtatg gcatgatagc gcccggaaga gagtcaattc agggtggtga atgtgaaacc
     2161 agtaacgtta tacgatgtcg cagagtatgc cggtgtctct tatcagaccg tttcccgcgt
     2221 ggtgaaccag gccagccacg tttctgcgaa aacgcgggaa aaagtggaag cggcgatggc
     2281 ggagctgaat tacattccca accgcgtggc acaacaactg gcgggcaaac agtcgttgct
     2341 gattggcgtt gccacctcca gtctggccct gcacgcgccg tcgcaaattg tcgcggcgat
     2401 taaatctcgc gccgatcaac tgggtgccag cgtggtggtg tcgatggtag aacgaagcgg
     2461 cgtcgaagcc tgtaaagcgg cggtgcacaa tcttctcgcg caacgcgtca gtgggctgat
     2521 cattaactat ccgctggatg accaggatgc cattgctgtg gaagctgcct gcactaatgt
     2581 tccggcgtta tttcttgatg tctctgacca gacacccatc aacagtatta ttttctccca
     2641 tgaagacggt acgcgactgg gcgtggagca tctggtcgca ttgggtcacc agcaaatcgc
     2701 gctgttagcg ggcccattaa gttctgtctc ggcgcgtctg cgtctggctg gctggcataa
     2761 atatctcact cgcaatcaaa ttcagccgat agcggaacgg gaaggcgact ggagtgccat
     2821 gtccggtttt caacaaacca tgcaaatgct gaatgagggc atcgttccca ctgcgatgct
     2881 ggttgccaac gatcagatgg cgctgggcgc aatgcgcgcc attaccgagt ccgggctgcg
     2941 cgttggtgcg gatatctcgg tagtgggata cgacgatacc gaagacagct catgttatat
     3001 cccgccgtta accaccatca aacaggattt tcgcctgctg gggcaaacca gcgtggaccg
     3061 cttgctgcaa ctctctcagg gccaggcggt gaagggcaat cagctgttgc ccgtctcact
     3121 ggtgaaaaga aaaaccaccc tggcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     3181 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
     3241 acgcaattaa tgtaagttag ctcactcatt aggcaccggg atctcgaccg atgcccttga
     3301 gagccttcaa cccagtcagc tccttccggt gggcgcgggg catgactatc gtcgccgcac
     3361 ttatgactgt cttctttatc atgcaactcg taggacaggt gccggcagcg ctctgggtca
     3421 ttttcggcga ggaccgcttt cgctggagcg cgacgatgat cggcctgtcg cttgcggtat
     3481 tcggaatctt gcacgccctc gctcaagcct tcgtcactgg tcccgccacc aaacgtttcg
     3541 gcgagaagca ggccattatc gccggcatgg cggccccacg ggtgcgcatg atcgtgctcc
     3601 tgtcgttgag gacccggcta ggctggcggg gttgccttac tggttagcag aatgaatcac
     3661 cgatacgcga gcgaacgtga agcgactgct gctgcaaaac gtctgcgacc tgagcaacaa
     3721 catgaatggt cttcggtttc cgtgtttcgt aaagtctgga aacgcggaag tcagcgccct
     3781 gcaccattat gttccggatc tgcatcgcag gatgctgctg gctaccctgt ggaacaccta
     3841 catctgtatt aacgaagcgc tggcattgac cctgagtgat ttttctctgg tcccgccgca
     3901 tccataccgc cagttgttta ccctcacaac gttccagtaa ccgggcatgt tcatcatcag
     3961 taacccgtat cgtgagcatc ctctctcgtt tcatcggtat cattaccccc atgaacagaa
     4021 atccccctta cacggaggca tcagtgacca aacaggaaaa aaccgccctt aacatggccc
     4081 gctttatcag aagccagaca ttaacgcttc tggagaaact caacgagctg gacgcggatg
     4141 aacaggcaga catctgtgaa tcgcttcacg accacgctga tgagctttac cgcagctgcc
     4201 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
     4261 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
     4321 ttggcgggtg tcggggcgca gccatgaccc agtcacgtag cgatagcgga gtgtatactg
     4381 gcttaactat gcggcatcag agcagattgt actgagagtg caccatatat gcggtgtgaa
     4441 ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc gctcttccgc ttcctcgctc
     4501 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
     4561 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
     4621 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
     4681 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
     4741 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
     4801 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
     4861 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
     4921 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
     4981 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
     5041 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
     5101 agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
     5161 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
     5221 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
     5281 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
     5341 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
     5401 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
     5461 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
     5521 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg
     5581 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
     5641 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
     5701 tcgccagtta atagtttgcg caacgttgtt gccattgctg caggcatcgt ggtgtcacgc
     5761 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
     5821 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
     5881 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
     5941 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
     6001 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca
     6061 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
     6121 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
     6181 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
     6241 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
     6301 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
     6361 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgaaatt
     6421 gtaaacgtta atattttgtt aaaattcgcg ttaaattttt gttaaatcag ctcatttttt
     6481 aaccaatagg ccgaaatcgg caaaatccct tataaatcaa aagaatagac cgagataggg
     6541 ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga ctccaacgtc
     6601 aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc accctaatca
     6661 agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg gagcccccga
     6721 tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa
     6781 ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc
     6841 gccgcgctta atgcgccgct acagggcgcg tcccattcgc ca
//
