LOCUS       15_pET-21a(+)           7083 bp ds-DNA     circular     15-FEB-2019
DEFINITION  .
KEYWORDS    "creator:Novagen (EMD Millipore)" "marker:AmpR"                    
            "organism:Escherichia coli"
FEATURES             Location/Qualifiers
     rep_origin      complement(4866..5454)
                     /label="ori"
     misc_feature    join(7069..7083,1..25)
                     /label="Feature 16"
     promoter        2276..2353
                     /label="lacI promoter"
     protein_bind    1924..1948
                     /label="lac operator"
     misc_feature    complement(159..1879)
                     /label="ProRS"
     terminator      26..73
                     /label="T7 terminator"
     RBS             1888..1893
                     /label="RBS"
     CDS             4245..4436
                     /label="rop"
     promoter        complement(6486..6590)
                     /label="AmpR promoter"
     CDS             complement(140..157)
                     /label="6xHis"
     CDS             complement(6417..6485)
                     /label="AmpR"
     CDS             2354..3436
                     /label="lacI"
     rep_origin      complement(6617..7072)
                     /label="f1 ori"
     misc_feature    158..158
                     /label="MCS"
     CDS             complement(5625..6416)
                     /label="AmpR"
     promoter        complement(1949..1967)
                     /label="T7 promoter"
     misc_feature    join(7069..7083,1..23)
                     /label="Feature 15"
ORIGIN
        1 ATCCGGATAT AGTTCCTCCT TTCAGCAAAA AACCCCTCAA GACCCGTTTA GAGGCCCCAA
       61 GGGGTTATGC TAGTTATTGC TCAGCGGTGG CAGCAGCCAA CTCAGCTTCC TTTCGGGCTT
      121 TGTTAGCAGC CGGATCTCAG TGGTGGTGGT GGTGGTGCTC GAGGCCTTTA ATCTGTTTCA
      181 CCAGATATTC GACGATGTCA CCAGTCTTAA TTAACTGTTT CTCGCCGTTG CGACGATATT
      241 TATATTCGAT ATCGTCGTTG TCGAGGTTAC GGTCGCCCAG CACAATAGTG TGCGGAATAC
      301 CGATCAGTTC CATATCAGCA AACATCACGC CCGGACGCTC TTTGCGGTCA TCCAGCAGCA
      361 CTTCGATACC TTGTGCACGC AGTTCGCTGT ACAGTTTCTC AGCAAGCTCT TGTACGCGGA
      421 AGGATTTGTG CATGTTCATC GGCAGAATCG CCACCTGGAA CGGCGCGATA GCGTCAGGCC
      481 ATACGATGCC TCGTTCGTCG TAGTTCTGCT CAATCGCCGC AGCTACCACA CGCGTTACCC
      541 CGATACCGTA GCAACCCATC GTCAGGATTT GGTTACGGCC ATCTTCACCC TGTACGGAGG
      601 CTTTCAGTGC TTCGGAGTAC TTGGTACCCA GCTGGAAGAT GTGACCAACT TCGATACCAC
      661 GTTTGATCAG CAGCCTACCC TGGCCATCCG GGCTTGGATC GCCAGCCACC ACGTTACGGA
      721 TATCTGCAAC TTCCGGGGTA GCGACATCGC GATCCCAGTT GATGCCGAAG TAGTGTTTAC
      781 CATCGATGTT AGCACCAGCA GCGAAATCAC TCATCGCCGC AACGGTACGG TCAATCACCA
      841 CCGGAATCGG CATGTTTACC GGACCCAGTG AACCCGGACC GGCTTTAACC ACGGCACGAA
      901 TTTCTTCTTC GGTCGCGAAA GTCAGCGGGC TTGCAACCTG CGGCAGTTTT TCTGCTTTAA
      961 CTTCGTTCAG CTCGTGATCA CCGCGCACCA GCAGCGCAAC CTGCGGGAAG CTGCTGCCTT
     1021 CAACCGCTTT AACCAGCAGA GTCTTAACCG TTTTCTCAAT CGGCAGATTG AACTGTTCAA
     1081 CCAGTTCCGC GATGGTTTTC GCGTTCGGCG TATCAACCAG CGTCATTTCC TGGGTAGCAG
     1141 CAGCGCGCGG TTCTTTCGGC GCGATAGCTT CTGCCAGTTC AATGTTCGCT GCATAGTCAG
     1201 AGGTGTCGGA GAAGACCACA TCGTCTTCAC CGCTCTGCGC CAGCACCTGG AATTCGTGAG
     1261 AGGCGCTGCC GCCGATAGAA CCGGTGTCGG CTTGTACGGC GCGGAAATCC AGCCCCATGC
     1321 GGCTGAAGAT TTTGCTGTAG GCCGCATACA TTGCATCGTA GGTTTCCTGC AGGGATTCCT
     1381 GAGAAGTATG GAAAGAGTAA GCATCTTTCA TCAGGAATTC GCGGGAACGC ATGACGCCGA
     1441 AACGCGGACG CACTTCGTCG CGGAACTTGG TCTGGATCTG ATAGAAGTTC AGCGGCAGCT
     1501 GTTTGTAAGA GCTAAGCTCG TTACGAATCA GGTCAGTGAT AACTTCTTCA TGAGTTGGGC
     1561 CGAGTACGAA CGGACGCTCG CCACGGTCAA CAAAACGCAG CAGTTCCGGA CCGTACTGTT
     1621 CCCAACGACC ACTCTCTTGC CACAAATCGG CTGGCTGAAC CACCGGCATC GACACCTCGA
     1681 TCGCACCGGC GTTGTTCATC TCTTCACGCA CGATGTTTTC GACTTTTTTC AGAACGCGCA
     1741 CGCCGGTCGG CAGCCAGGTA TATAACCCGG AGGCCAGCTT GCGGATCATC CCGGCGCGCA
     1801 GCATCAGCTG ATGGCTGATC ACCTCGGCGT CGGCAGGTGT CTCCTTGAGA GTGGAGAGCA
     1861 GGTATTGGCT AGTACGCATA TGTATATCTC CTTCTTAAAG TTAAACAAAA TTATTTCTAG
     1921 AGGGGAATTG TTATCCGCTC ACAATTCCCC TATAGTGAGT CGTATTAATT TCGCGGGATC
     1981 GAGATCTCGA TCCTCTACGC CGGACGCATC GTGGCCGGCA TCACCGGCGC CACAGGTGCG
     2041 GTTGCTGGCG CCTATATCGC CGACATCACC GATGGGGAAG ATCGGGCTCG CCACTTCGGG
     2101 CTCATGAGCG CTTGTTTCGG CGTGGGTATG GTGGCAGGCC CCGTGGCCGG GGGACTGTTG
     2161 GGCGCCATCT CCTTGCATGC ACCATTCCTT GCGGCGGCGG TGCTCAACGG CCTCAACCTA
     2221 CTACTGGGCT GCTTCCTAAT GCAGGAGTCG CATAAGGGAG AGCGTCGAGA TCCCGGACAC
     2281 CATCGAATGG CGCAAAACCT TTCGCGGTAT GGCATGATAG CGCCCGGAAG AGAGTCAATT
     2341 CAGGGTGGTG AATGTGAAAC CAGTAACGTT ATACGATGTC GCAGAGTATG CCGGTGTCTC
     2401 TTATCAGACC GTTTCCCGCG TGGTGAACCA GGCCAGCCAC GTTTCTGCGA AAACGCGGGA
     2461 AAAAGTGGAA GCGGCGATGG CGGAGCTGAA TTACATTCCC AACCGCGTGG CACAACAACT
     2521 GGCGGGCAAA CAGTCGTTGC TGATTGGCGT TGCCACCTCC AGTCTGGCCC TGCACGCGCC
     2581 GTCGCAAATT GTCGCGGCGA TTAAATCTCG CGCCGATCAA CTGGGTGCCA GCGTGGTGGT
     2641 GTCGATGGTA GAACGAAGCG GCGTCGAAGC CTGTAAAGCG GCGGTGCACA ATCTTCTCGC
     2701 GCAACGCGTC AGTGGGCTGA TCATTAACTA TCCGCTGGAT GACCAGGATG CCATTGCTGT
     2761 GGAAGCTGCC TGCACTAATG TTCCGGCGTT ATTTCTTGAT GTCTCTGACC AGACACCCAT
     2821 CAACAGTATT ATTTTCTCCC ATGAAGACGG TACGCGACTG GGCGTGGAGC ATCTGGTCGC
     2881 ATTGGGTCAC CAGCAAATCG CGCTGTTAGC GGGCCCATTA AGTTCTGTCT CGGCGCGTCT
     2941 GCGTCTGGCT GGCTGGCATA AATATCTCAC TCGCAATCAA ATTCAGCCGA TAGCGGAACG
     3001 GGAAGGCGAC TGGAGTGCCA TGTCCGGTTT TCAACAAACC ATGCAAATGC TGAATGAGGG
     3061 CATCGTTCCC ACTGCGATGC TGGTTGCCAA CGATCAGATG GCGCTGGGCG CAATGCGCGC
     3121 CATTACCGAG TCCGGGCTGC GCGTTGGTGC GGATATCTCG GTAGTGGGAT ACGACGATAC
     3181 CGAAGACAGC TCATGTTATA TCCCGCCGTT AACCACCATC AAACAGGATT TTCGCCTGCT
     3241 GGGGCAAACC AGCGTGGACC GCTTGCTGCA ACTCTCTCAG GGCCAGGCGG TGAAGGGCAA
     3301 TCAGCTGTTG CCCGTCTCAC TGGTGAAAAG AAAAACCACC CTGGCGCCCA ATACGCAAAC
     3361 CGCCTCTCCC CGCGCGTTGG CCGATTCATT AATGCAGCTG GCACGACAGG TTTCCCGACT
     3421 GGAAAGCGGG CAGTGAGCGC AACGCAATTA ATGTAAGTTA GCTCACTCAT TAGGCACCGG
     3481 GATCTCGACC GATGCCCTTG AGAGCCTTCA ACCCAGTCAG CTCCTTCCGG TGGGCGCGGG
     3541 GCATGACTAT CGTCGCCGCA CTTATGACTG TCTTCTTTAT CATGCAACTC GTAGGACAGG
     3601 TGCCGGCAGC GCTCTGGGTC ATTTTCGGCG AGGACCGCTT TCGCTGGAGC GCGACGATGA
     3661 TCGGCCTGTC GCTTGCGGTA TTCGGAATCT TGCACGCCCT CGCTCAAGCC TTCGTCACTG
     3721 GTCCCGCCAC CAAACGTTTC GGCGAGAAGC AGGCCATTAT CGCCGGCATG GCGGCCCCAC
     3781 GGGTGCGCAT GATCGTGCTC CTGTCGTTGA GGACCCGGCT AGGCTGGCGG GGTTGCCTTA
     3841 CTGGTTAGCA GAATGAATCA CCGATACGCG AGCGAACGTG AAGCGACTGC TGCTGCAAAA
     3901 CGTCTGCGAC CTGAGCAACA ACATGAATGG TCTTCGGTTT CCGTGTTTCG TAAAGTCTGG
     3961 AAACGCGGAA GTCAGCGCCC TGCACCATTA TGTTCCGGAT CTGCATCGCA GGATGCTGCT
     4021 GGCTACCCTG TGGAACACCT ACATCTGTAT TAACGAAGCG CTGGCATTGA CCCTGAGTGA
     4081 TTTTTCTCTG GTCCCGCCGC ATCCATACCG CCAGTTGTTT ACCCTCACAA CGTTCCAGTA
     4141 ACCGGGCATG TTCATCATCA GTAACCCGTA TCGTGAGCAT CCTCTCTCGT TTCATCGGTA
     4201 TCATTACCCC CATGAACAGA AATCCCCCTT ACACGGAGGC ATCAGTGACC AAACAGGAAA
     4261 AAACCGCCCT TAACATGGCC CGCTTTATCA GAAGCCAGAC ATTAACGCTT CTGGAGAAAC
     4321 TCAACGAGCT GGACGCGGAT GAACAGGCAG ACATCTGTGA ATCGCTTCAC GACCACGCTG
     4381 ATGAGCTTTA CCGCAGCTGC CTCGCGCGTT TCGGTGATGA CGGTGAAAAC CTCTGACACA
     4441 TGCAGCTCCC GGAGACGGTC ACAGCTTGTC TGTAAGCGGA TGCCGGGAGC AGACAAGCCC
     4501 GTCAGGGCGC GTCAGCGGGT GTTGGCGGGT GTCGGGGCGC AGCCATGACC CAGTCACGTA
     4561 GCGATAGCGG AGTGTATACT GGCTTAACTA TGCGGCATCA GAGCAGATTG TACTGAGAGT
     4621 GCACCATATA TGCGGTGTGA AATACCGCAC AGATGCGTAA GGAGAAAATA CCGCATCAGG
     4681 CGCTCTTCCG CTTCCTCGCT CACTGACTCG CTGCGCTCGG TCGTTCGGCT GCGGCGAGCG
     4741 GTATCAGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG AATCAGGGGA TAACGCAGGA
     4801 AAGAACATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC CGCGTTGCTG
     4861 GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG
     4921 AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC
     4981 GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG
     5041 GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT
     5101 CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC
     5161 GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC
     5221 ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG
     5281 TGGCCTAACT ACGGCTACAC TAGAAGGACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA
     5341 GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC
     5401 GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT
     5461 CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT
     5521 TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT
     5581 TTTAAATCAA TCTAAAGTAT ATATGAGTAA ACTTGGTCTG ACAGTTACCA ATGCTTAATC
     5641 AGTGAGGCAC CTATCTCAGC GATCTGTCTA TTTCGTTCAT CCATAGTTGC CTGACTCCCC
     5701 GTCGTGTAGA TAACTACGAT ACGGGAGGGC TTACCATCTG GCCCCAGTGC TGCAATGATA
     5761 CCGCGAGACC CACGCTCACC GGCTCCAGAT TTATCAGCAA TAAACCAGCC AGCCGGAAGG
     5821 GCCGAGCGCA GAAGTGGTCC TGCAACTTTA TCCGCCTCCA TCCAGTCTAT TAATTGTTGC
     5881 CGGGAAGCTA GAGTAAGTAG TTCGCCAGTT AATAGTTTGC GCAACGTTGT TGCCATTGCT
     5941 GCAGGCATCG TGGTGTCACG CTCGTCGTTT GGTATGGCTT CATTCAGCTC CGGTTCCCAA
     6001 CGATCAAGGC GAGTTACATG ATCCCCCATG TTGTGCAAAA AAGCGGTTAG CTCCTTCGGT
     6061 CCTCCGATCG TTGTCAGAAG TAAGTTGGCC GCAGTGTTAT CACTCATGGT TATGGCAGCA
     6121 CTGCATAATT CTCTTACTGT CATGCCATCC GTAAGATGCT TTTCTGTGAC TGGTGAGTAC
     6181 TCAACCAAGT CATTCTGAGA ATAGTGTATG CGGCGACCGA GTTGCTCTTG CCCGGCGTCA
     6241 ATACGGGATA ATACCGCGCC ACATAGCAGA ACTTTAAAAG TGCTCATCAT TGGAAAACGT
     6301 TCTTCGGGGC GAAAACTCTC AAGGATCTTA CCGCTGTTGA GATCCAGTTC GATGTAACCC
     6361 ACTCGTGCAC CCAACTGATC TTCAGCATCT TTTACTTTCA CCAGCGTTTC TGGGTGAGCA
     6421 AAAACAGGAA GGCAAAATGC CGCAAAAAAG GGAATAAGGG CGACACGGAA ATGTTGAATA
     6481 CTCATACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG TCTCATGAGC
     6541 GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACATTTCCC
     6601 CGAAAAGTGC CACCTGAAAT TGTAAACGTT AATATTTTGT TAAAATTCGC GTTAAATTTT
     6661 TGTTAAATCA GCTCATTTTT TAACCAATAG GCCGAAATCG GCAAAATCCC TTATAAATCA
     6721 AAAGAATAGA CCGAGATAGG GTTGAGTGTT GTTCCAGTTT GGAACAAGAG TCCACTATTA
     6781 AAGAACGTGG ACTCCAACGT CAAAGGGCGA AAAACCGTCT ATCAGGGCGA TGGCCCACTA
     6841 CGTGAACCAT CACCCTAATC AAGTTTTTTG GGGTCGAGGT GCCGTAAAGC ACTAAATCGG
     6901 AACCCTAAAG GGAGCCCCCG ATTTAGAGCT TGACGGGGAA AGCCGGCGAA CGTGGCGAGA
     6961 AAGGAAGGGA AGAAAGCGAA AGGAGCGGGC GCTAGGGCGC TGGCAAGTGT AGCGGTCACG
     7021 CTGCGCGTAA CCACCACACC CGCCGCGCTT AATGCGCCGC TACAGGGCGC GTCCCATTCG
     7081 CCA
//