LOCUS       16_pET-21a(+)           6634 bp ds-DNA     circular     15-FEB-2019
DEFINITION  .
KEYWORDS    "creator:Novagen (EMD Millipore)" "marker:AmpR"                    
            "organism:Escherichia coli"
FEATURES             Location/Qualifiers
     rep_origin      complement(4417..5005)
                     /label="ori"
     promoter        complement(6037..6141)
                     /label="AmpR promoter"
     CDS             1905..2987
                     /label="lacI"
     CDS             complement(5176..5967)
                     /label="AmpR"
     protein_bind    1475..1499
                     /label="lac operator"
     rep_origin      complement(6168..6623)
                     /label="f1 ori"
     CDS             3796..3987
                     /label="rop"
     promoter        complement(1500..1518)
                     /label="T7 promoter"
     misc_feature    complement(159..1453)
                     /label="SerRS"
     terminator      26..73
                     /label="T7 terminator"
     misc_feature    join(6620..6634,1..23)
                     /label="Feature 15"
     promoter        1827..1904
                     /label="lacI promoter"
     misc_feature    join(6620..6634,1..25)
                     /label="Feature 16"
     CDS             complement(140..157)
                     /label="6xHis"
     misc_feature    158..158
                     /label="MCS"
     CDS             complement(5968..6036)
                     /label="AmpR"
ORIGIN
        1 ATCCGGATAT AGTTCCTCCT TTCAGCAAAA AACCCCTCAA GACCCGTTTA GAGGCCCCAA
       61 GGGGTTATGC TAGTTATTGC TCAGCGGTGG CAGCAGCCAA CTCAGCTTCC TTTCGGGCTT
      121 TGTTAGCAGC CGGATCTCAG TGGTGGTGGT GGTGGTGCTC GAGGCCAATA TATTCCAGTC
      181 CGTTCATATA CGGACGCAGA ACTTCTGGTA CTTCAATACG ACCATCAGCC TGCTGATAGT
      241 TTTCCATTAC TGCAACCAGC GTACGACCAA CAGCCAGACC AGAACCGTTC AGGGTATGAA
      301 CCAGACGGGT TTTCTTGTCC GACTTGCTGC GGCAACGTGC CTGCATACGA CGTGCCTGGA
      361 AATCCCAAAC GTTGGAGCAG GAAGAGATCT CACGGTAGGT GTTCTGTGCC GGGATCCATA
      421 CTTCCAGGTC GTAAGTTTTG CAAGCGCCAA AGCCCATGTC GCCAGTGCAA AGGATGATTT
      481 TACGGTACGG CAGGCCCAGC AACTGCAGGA CTTTTTCTGC ATGACCAGTC ATCTCTTCCA
      541 GCGCCGCCAT TGAGTCTTCT GGGCGCACGA TCTGCACCAT TTCAACTTTG TCGAACTGGT
      601 GCATACGGAT CAGACCACGG GTGTCACGAC CATATGAACC GGCTTCAGAA CGGAAGCATG
      661 GGGTGTGGGC GGTCATCTTA ATTGGCAGAT CATCTTCATC GATGATTTCA CCGCGCACCA
      721 GGTTAGTCAG CGGAACTTCT GCCGTTGGGA TCAGCGCATA GTTACTGGTG TCTGCTTCTT
      781 CTTCCAGCGG ACGAGTATGG AACAGATCGC CAGCAAATTT CGGCAGTTGA CCCGTACCGT
      841 ACAGCGTGTC CTGGTTAACC AGGTACGGAA CATAGTTCTC ACTGTAGCCA TGCTGTTCGG
      901 TATGCAGATC CAGCATAAAC TGCGACAGTG CGCGGTGCAT GCGAGCAATC TGCCCTTTCA
      961 TTACCACAAA GCGGGAACCA GTCAGCTTAA CTGCAGCTGC AAAGTCGAGG CCAGAGTGCA
     1021 TTTCACCCAG CGTCACGTGG TCACGAACTT CAAAGTCAAA CTCACGCGGG GTACCCCAGC
     1081 GGCTGACTTC AACGTTGTCA TTTTCGTCTT TACCTACCGG CACTTCATCT GCAGGCAGGT
     1141 TAGGGATGGT CAGCGCGATA TCGCGAATTT CAGCCTGTAA AGCATCCAGC TCGGCTTTTG
     1201 CTGCATCCAG CTCTTCGCCC AGTTTGTTCA CTTCCAGACG TAAAGGCTCG ATATCTTCCC
     1261 CGCGCGCTTT CGCCTGGCCA ATGGATTTCG ATCGGGAGTT ACGCTCCGCT TGCAGGTTTT
     1321 CCGTTTTGAC CTGCAATACT TTACGACGCT CTTCAAGAGC GCCCAGCTTA TCTACATCCA
     1381 GCTTAAAGCC CCGGCGTGCC AGTTTTTCAG CGACTGCGTC TGGCTCATTA CGCAGCAGAT
     1441 TGGGATCGAG CATGCTTATA TCTCCTTCTA GAGGGGAATT GTTATCCGCT CACAATTCCC
     1501 CTATAGTGAG TCGTATTAAT TTCGCGGGAT CGAGATCTCG ATCCTCTACG CCGGACGCAT
     1561 CGTGGCCGGC ATCACCGGCG CCACAGGTGC GGTTGCTGGC GCCTATATCG CCGACATCAC
     1621 CGATGGGGAA GATCGGGCTC GCCACTTCGG GCTCATGAGC GCTTGTTTCG GCGTGGGTAT
     1681 GGTGGCAGGC CCCGTGGCCG GGGGACTGTT GGGCGCCATC TCCTTGCATG CACCATTCCT
     1741 TGCGGCGGCG GTGCTCAACG GCCTCAACCT ACTACTGGGC TGCTTCCTAA TGCAGGAGTC
     1801 GCATAAGGGA GAGCGTCGAG ATCCCGGACA CCATCGAATG GCGCAAAACC TTTCGCGGTA
     1861 TGGCATGATA GCGCCCGGAA GAGAGTCAAT TCAGGGTGGT GAATGTGAAA CCAGTAACGT
     1921 TATACGATGT CGCAGAGTAT GCCGGTGTCT CTTATCAGAC CGTTTCCCGC GTGGTGAACC
     1981 AGGCCAGCCA CGTTTCTGCG AAAACGCGGG AAAAAGTGGA AGCGGCGATG GCGGAGCTGA
     2041 ATTACATTCC CAACCGCGTG GCACAACAAC TGGCGGGCAA ACAGTCGTTG CTGATTGGCG
     2101 TTGCCACCTC CAGTCTGGCC CTGCACGCGC CGTCGCAAAT TGTCGCGGCG ATTAAATCTC
     2161 GCGCCGATCA ACTGGGTGCC AGCGTGGTGG TGTCGATGGT AGAACGAAGC GGCGTCGAAG
     2221 CCTGTAAAGC GGCGGTGCAC AATCTTCTCG CGCAACGCGT CAGTGGGCTG ATCATTAACT
     2281 ATCCGCTGGA TGACCAGGAT GCCATTGCTG TGGAAGCTGC CTGCACTAAT GTTCCGGCGT
     2341 TATTTCTTGA TGTCTCTGAC CAGACACCCA TCAACAGTAT TATTTTCTCC CATGAAGACG
     2401 GTACGCGACT GGGCGTGGAG CATCTGGTCG CATTGGGTCA CCAGCAAATC GCGCTGTTAG
     2461 CGGGCCCATT AAGTTCTGTC TCGGCGCGTC TGCGTCTGGC TGGCTGGCAT AAATATCTCA
     2521 CTCGCAATCA AATTCAGCCG ATAGCGGAAC GGGAAGGCGA CTGGAGTGCC ATGTCCGGTT
     2581 TTCAACAAAC CATGCAAATG CTGAATGAGG GCATCGTTCC CACTGCGATG CTGGTTGCCA
     2641 ACGATCAGAT GGCGCTGGGC GCAATGCGCG CCATTACCGA GTCCGGGCTG CGCGTTGGTG
     2701 CGGATATCTC GGTAGTGGGA TACGACGATA CCGAAGACAG CTCATGTTAT ATCCCGCCGT
     2761 TAACCACCAT CAAACAGGAT TTTCGCCTGC TGGGGCAAAC CAGCGTGGAC CGCTTGCTGC
     2821 AACTCTCTCA GGGCCAGGCG GTGAAGGGCA ATCAGCTGTT GCCCGTCTCA CTGGTGAAAA
     2881 GAAAAACCAC CCTGGCGCCC AATACGCAAA CCGCCTCTCC CCGCGCGTTG GCCGATTCAT
     2941 TAATGCAGCT GGCACGACAG GTTTCCCGAC TGGAAAGCGG GCAGTGAGCG CAACGCAATT
     3001 AATGTAAGTT AGCTCACTCA TTAGGCACCG GGATCTCGAC CGATGCCCTT GAGAGCCTTC
     3061 AACCCAGTCA GCTCCTTCCG GTGGGCGCGG GGCATGACTA TCGTCGCCGC ACTTATGACT
     3121 GTCTTCTTTA TCATGCAACT CGTAGGACAG GTGCCGGCAG CGCTCTGGGT CATTTTCGGC
     3181 GAGGACCGCT TTCGCTGGAG CGCGACGATG ATCGGCCTGT CGCTTGCGGT ATTCGGAATC
     3241 TTGCACGCCC TCGCTCAAGC CTTCGTCACT GGTCCCGCCA CCAAACGTTT CGGCGAGAAG
     3301 CAGGCCATTA TCGCCGGCAT GGCGGCCCCA CGGGTGCGCA TGATCGTGCT CCTGTCGTTG
     3361 AGGACCCGGC TAGGCTGGCG GGGTTGCCTT ACTGGTTAGC AGAATGAATC ACCGATACGC
     3421 GAGCGAACGT GAAGCGACTG CTGCTGCAAA ACGTCTGCGA CCTGAGCAAC AACATGAATG
     3481 GTCTTCGGTT TCCGTGTTTC GTAAAGTCTG GAAACGCGGA AGTCAGCGCC CTGCACCATT
     3541 ATGTTCCGGA TCTGCATCGC AGGATGCTGC TGGCTACCCT GTGGAACACC TACATCTGTA
     3601 TTAACGAAGC GCTGGCATTG ACCCTGAGTG ATTTTTCTCT GGTCCCGCCG CATCCATACC
     3661 GCCAGTTGTT TACCCTCACA ACGTTCCAGT AACCGGGCAT GTTCATCATC AGTAACCCGT
     3721 ATCGTGAGCA TCCTCTCTCG TTTCATCGGT ATCATTACCC CCATGAACAG AAATCCCCCT
     3781 TACACGGAGG CATCAGTGAC CAAACAGGAA AAAACCGCCC TTAACATGGC CCGCTTTATC
     3841 AGAAGCCAGA CATTAACGCT TCTGGAGAAA CTCAACGAGC TGGACGCGGA TGAACAGGCA
     3901 GACATCTGTG AATCGCTTCA CGACCACGCT GATGAGCTTT ACCGCAGCTG CCTCGCGCGT
     3961 TTCGGTGATG ACGGTGAAAA CCTCTGACAC ATGCAGCTCC CGGAGACGGT CACAGCTTGT
     4021 CTGTAAGCGG ATGCCGGGAG CAGACAAGCC CGTCAGGGCG CGTCAGCGGG TGTTGGCGGG
     4081 TGTCGGGGCG CAGCCATGAC CCAGTCACGT AGCGATAGCG GAGTGTATAC TGGCTTAACT
     4141 ATGCGGCATC AGAGCAGATT GTACTGAGAG TGCACCATAT ATGCGGTGTG AAATACCGCA
     4201 CAGATGCGTA AGGAGAAAAT ACCGCATCAG GCGCTCTTCC GCTTCCTCGC TCACTGACTC
     4261 GCTGCGCTCG GTCGTTCGGC TGCGGCGAGC GGTATCAGCT CACTCAAAGG CGGTAATACG
     4321 GTTATCCACA GAATCAGGGG ATAACGCAGG AAAGAACATG TGAGCAAAAG GCCAGCAAAA
     4381 GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC GCCCCCCTGA
     4441 CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG
     4501 ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT
     4561 TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC ATAGCTCACG
     4621 CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC
     4681 CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT
     4741 AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA
     4801 TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGGAC
     4861 AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC
     4921 TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT
     4981 TACGCGCAGA AAAAAAGGAT CTCAAGAAGA TCCTTTGATC TTTTCTACGG GGTCTGACGC
     5041 TCAGTGGAAC GAAAACTCAC GTTAAGGGAT TTTGGTCATG AGATTATCAA AAAGGATCTT
     5101 CACCTAGATC CTTTTAAATT AAAAATGAAG TTTTAAATCA ATCTAAAGTA TATATGAGTA
     5161 AACTTGGTCT GACAGTTACC AATGCTTAAT CAGTGAGGCA CCTATCTCAG CGATCTGTCT
     5221 ATTTCGTTCA TCCATAGTTG CCTGACTCCC CGTCGTGTAG ATAACTACGA TACGGGAGGG
     5281 CTTACCATCT GGCCCCAGTG CTGCAATGAT ACCGCGAGAC CCACGCTCAC CGGCTCCAGA
     5341 TTTATCAGCA ATAAACCAGC CAGCCGGAAG GGCCGAGCGC AGAAGTGGTC CTGCAACTTT
     5401 ATCCGCCTCC ATCCAGTCTA TTAATTGTTG CCGGGAAGCT AGAGTAAGTA GTTCGCCAGT
     5461 TAATAGTTTG CGCAACGTTG TTGCCATTGC TGCAGGCATC GTGGTGTCAC GCTCGTCGTT
     5521 TGGTATGGCT TCATTCAGCT CCGGTTCCCA ACGATCAAGG CGAGTTACAT GATCCCCCAT
     5581 GTTGTGCAAA AAAGCGGTTA GCTCCTTCGG TCCTCCGATC GTTGTCAGAA GTAAGTTGGC
     5641 CGCAGTGTTA TCACTCATGG TTATGGCAGC ACTGCATAAT TCTCTTACTG TCATGCCATC
     5701 CGTAAGATGC TTTTCTGTGA CTGGTGAGTA CTCAACCAAG TCATTCTGAG AATAGTGTAT
     5761 GCGGCGACCG AGTTGCTCTT GCCCGGCGTC AATACGGGAT AATACCGCGC CACATAGCAG
     5821 AACTTTAAAA GTGCTCATCA TTGGAAAACG TTCTTCGGGG CGAAAACTCT CAAGGATCTT
     5881 ACCGCTGTTG AGATCCAGTT CGATGTAACC CACTCGTGCA CCCAACTGAT CTTCAGCATC
     5941 TTTTACTTTC ACCAGCGTTT CTGGGTGAGC AAAAACAGGA AGGCAAAATG CCGCAAAAAA
     6001 GGGAATAAGG GCGACACGGA AATGTTGAAT ACTCATACTC TTCCTTTTTC AATATTATTG
     6061 AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA TTTGAATGTA TTTAGAAAAA
     6121 TAAACAAATA GGGGTTCCGC GCACATTTCC CCGAAAAGTG CCACCTGAAA TTGTAAACGT
     6181 TAATATTTTG TTAAAATTCG CGTTAAATTT TTGTTAAATC AGCTCATTTT TTAACCAATA
     6241 GGCCGAAATC GGCAAAATCC CTTATAAATC AAAAGAATAG ACCGAGATAG GGTTGAGTGT
     6301 TGTTCCAGTT TGGAACAAGA GTCCACTATT AAAGAACGTG GACTCCAACG TCAAAGGGCG
     6361 AAAAACCGTC TATCAGGGCG ATGGCCCACT ACGTGAACCA TCACCCTAAT CAAGTTTTTT
     6421 GGGGTCGAGG TGCCGTAAAG CACTAAATCG GAACCCTAAA GGGAGCCCCC GATTTAGAGC
     6481 TTGACGGGGA AAGCCGGCGA ACGTGGCGAG AAAGGAAGGG AAGAAAGCGA AAGGAGCGGG
     6541 CGCTAGGGCG CTGGCAAGTG TAGCGGTCAC GCTGCGCGTA ACCACCACAC CCGCCGCGCT
     6601 TAATGCGCCG CTACAGGGCG CGTCCCATTC GCCA
//