LOCUS       21_pQE30_IF1            3642 bp ds-DNA     circular     18-FEB-2019
DEFINITION  .
KEYWORDS    "creator:Barbora Lavickova" "marker:CmR, AmpR"                     
FEATURES             Location/Qualifiers
     rep_origin      complement(1818..2406)
                     /label="ori"
     CDS             complement(3369..3437)
                     /label="AmpR"
     misc_feature    1492..1632
                     /label="bom"
     CDS             127..144
                     /label="6xHis"
     gene            151..369
                     /label="IF1"
     protein_bind    30..46
                     /label="lac operator"
     promoter        31..47
                     /label="T5 promoter"
     promoter        48..54
                     /label="T5 promoter"
     CDS             527..1186
                     /label="CmR"
     promoter        complement(3438..3542)
                     /label="AmpR promoter"
     RBS             97..108
                     /label="RBS"
     terminator      389..483
                     /label="lambda t0 terminator"
     promoter        10..24
                     /label="T5 promoter"
     terminator      1251..1337
                     /label="rrnB T1 terminator"
     promoter        25..30
                     /label="T5 promoter"
     protein_bind    62..78
                     /label="lac operator"
     CDS             complement(2577..3368)
                     /label="AmpR"
ORIGIN
        1 CTCGAGAAAT CATAAAAAAT TTATTTGCTT TGTGAGCGGA TAACAATTAT AATAGATTCA
       61 ATTGTGAGCG GATAACAATT TCACACAGAA TTCATTAAAG AGGAGAAATT AACTATGAGA
      121 GGATCGCATC ACCATCACCA TCACGGATCC ATGGCCAAAG AAGACAATAT TGAAATGCAA
      181 GGTACCGTTC TTGAAACGTT GCCTAATACC ATGTTCCGCG TAGAGTTAGA AAACGGTCAC
      241 GTGGTTACTG CACACATCTC CGGTAAAATG CGCAAAAACT ACATCCGCAT CCTGACGGGC
      301 GACAAAGTGA CTGTTGAACT GACCCCGTAC GACCTGAGCA AAGGCCGCAT TGTCTTCCGT
      361 AGTCGCTGAA GCTAATTAGC TGAGCTTGGA CTCCTGTTGA TAGATCCAGT AATGACCTCA
      421 GAACTCCATC TGGATTTGTT CAGAACGCTC GGTTGCCGCC GGGCGTTTTT TATTGGTGAG
      481 AATCCAAGCT AGCTTGGCGA GATTTTCAGG AGCTAAGGAA GCTAAAATGG AGAAAAAAAT
      541 CACTGGATAT ACCACCGTTG ATATATCCCA ATGGCATCGT AAAGAACATT TTGAGGCATT
      601 TCAGTCAGTT GCTCAATGTA CCTATAACCA GACCGTTCAG CTGGATATTA CGGCCTTTTT
      661 AAAGACCGTA AAGAAAAATA AGCACAAGTT TTATCCGGCC TTTATTCACA TTCTTGCCCG
      721 CCTGATGAAT GCTCATCCGG AATTTCGTAT GGCAATGAAA GACGGTGAGC TGGTGATATG
      781 GGATAGTGTT CACCCTTGTT ACACCGTTTT CCATGAGCAA ACTGAAACGT TTTCATCGCT
      841 CTGGAGTGAA TACCACGACG ATTTCCGGCA GTTTCTACAC ATATATTCGC AAGATGTGGC
      901 GTGTTACGGT GAAAACCTGG CCTATTTCCC TAAAGGGTTT ATTGAGAATA TGTTTTTCGT
      961 CTCAGCCAAT CCCTGGGTGA GTTTCACCAG TTTTGATTTA AACGTGGCCA ATATGGACAA
     1021 CTTCTTCGCC CCCGTTTTCA CCATGGGCAA ATATTATACG CAAGGCGACA AGGTGCTGAT
     1081 GCCGCTGGCG ATTCAGGTTC ATCATGCCGT TTGTGATGGC TTCCATGTCG GCAGAATGCT
     1141 TAATGAATTA CAACAGTACT GCGATGAGTG GCAGGGCGGG GCGTAATTTT TTTAAGGCAG
     1201 TTATTGGTGC CCTTAAACGC CTGGGGTAAT GACTCTCTAG CTTGAGGCAT CAAATAAAAC
     1261 GAAAGGCTCA GTCGAAAGAC TGGGCCTTTC GTTTTATCTG TTGTTTGTCG GTGAACGCTC
     1321 TCCTGAGTAG GACAAATCCG CCCTCTAGAG CTGCCTCGCG CGTTTCGGTG ATGACGGTGA
     1381 AAACCTCTGA CACATGCAGC TCCCGGAGAC GGTCACAGCT TGTCTGTAAG CGGATGCCGG
     1441 GAGCAGACAA GCCCGTCAGG GCGCGTCAGC GGGTGTTGGC GGGTGTCGGG GCGCAGCCAT
     1501 GACCCAGTCA CGTAGCGATA GCGGAGTGTA TACTGGCTTA ACTATGCGGC ATCAGAGCAG
     1561 ATTGTACTGA GAGTGCACCA TATGCGGTGT GAAATACCGC ACAGATGCGT AAGGAGAAAA
     1621 TACCGCATCA GGCGCTCTTC CGCTTCCTCG CTCACTGACT CGCTGCGCTC GGTCGTTCGG
     1681 CTGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC AGAATCAGGG
     1741 GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG
     1801 GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA
     1861 CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT
     1921 GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC
     1981 TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA TCTCAGTTCG
     2041 GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA GCCCGACCGC
     2101 TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA
     2161 CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG TGCTACAGAG
     2221 TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGGA CAGTATTTGG TATCTGCGCT
     2281 CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG CAAACAAACC
     2341 ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG AAAAAAAGGA
     2401 TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA CGAAAACTCA
     2461 CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT CCTTTTAAAT
     2521 TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT AAACTTGGTC TGACAGTTAC
     2581 CAATGCTTAA TCAGTGAGGC ACCTATCTCA GCGATCTGTC TATTTCGTTC ATCCATAGTT
     2641 GCCTGACTCC CCGTCGTGTA GATAACTACG ATACGGGAGG GCTTACCATC TGGCCCCAGT
     2701 GCTGCAATGA TACCGCGAGA CCCACGCTCA CCGGCTCCAG ATTTATCAGC AATAAACCAG
     2761 CCAGCCGGAA GGGCCGAGCG CAGAAGTGGT CCTGCAACTT TATCCGCCTC CATCCAGTCT
     2821 ATTAATTGTT GCCGGGAAGC TAGAGTAAGT AGTTCGCCAG TTAATAGTTT GCGCAACGTT
     2881 GTTGCCATTG CTACAGGCAT CGTGGTGTCA CGCTCGTCGT TTGGTATGGC TTCATTCAGC
     2941 TCCGGTTCCC AACGATCAAG GCGAGTTACA TGATCCCCCA TGTTGTGCAA AAAAGCGGTT
     3001 AGCTCCTTCG GTCCTCCGAT CGTTGTCAGA AGTAAGTTGG CCGCAGTGTT ATCACTCATG
     3061 GTTATGGCAG CACTGCATAA TTCTCTTACT GTCATGCCAT CCGTAAGATG CTTTTCTGTG
     3121 ACTGGTGAGT ACTCAACCAA GTCATTCTGA GAATAGTGTA TGCGGCGACC GAGTTGCTCT
     3181 TGCCCGGCGT CAATACGGGA TAATACCGCG CCACATAGCA GAACTTTAAA AGTGCTCATC
     3241 ATTGGAAAAC GTTCTTCGGG GCGAAAACTC TCAAGGATCT TACCGCTGTT GAGATCCAGT
     3301 TCGATGTAAC CCACTCGTGC ACCCAACTGA TCTTCAGCAT CTTTTACTTT CACCAGCGTT
     3361 TCTGGGTGAG CAAAAACAGG AAGGCAAAAT GCCGCAAAAA AGGGAATAAG GGCGACACGG
     3421 AAATGTTGAA TACTCATACT CTTCCTTTTT CAATATTATT GAAGCATTTA TCAGGGTTAT
     3481 TGTCTCATGA GCGGATACAT ATTTGAATGT ATTTAGAAAA ATAAACAAAT AGGGGTTCCG
     3541 CGCACATTTC CCCGAAAAGT GCCACCTGAC GTCTAAGAAA CCATTATTAT CATGACATTA
     3601 ACCTATAAAA ATAGGCGTAT CACGAGGCCC TTTCGTCTTC AC
//