LOCUS       25-pQE-60               4597 bp ds-DNA     circular     18-FEB-2019
DEFINITION  .
KEYWORDS    "creator:Barbora" "marker:AmpR"                                    
FEATURES             Location/Qualifiers
     promoter        1347..1352
                     /label="T5 promoter"
     protein_bind    1384..1400
                     /label="lac operator"
     CDS             2621..2638
                     /label="6xHis"
     CDS             complement(257..1048)
                     /label="AmpR"
     terminator      2661..2755
                     /label="lambda t0 terminator"
     rep_origin      complement(join(4095..4597,1..86))
                     /label="ori"
     misc_feature    1433..2615
                     /label="EF-Tu"
     protein_bind    1352..1368
                     /label="lac operator"
     promoter        1370..1376
                     /label="T5 promoter"
     promoter        1353..1369
                     /label="T5 promoter"
     promoter        1332..1346
                     /label="T5 promoter"
     CDS             complement(1049..1117)
                     /label="AmpR"
     misc_feature    3769..3909
                     /label="bom"
     promoter        complement(1118..1222)
                     /label="AmpR promoter"
     terminator      3527..3613
                     /label="rrnB T1 terminator"
ORIGIN
        1 CTTGATCCGG CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA
       61 TTACGCGCAG AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG
      121 CTCAGTGGAA CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT
      181 TCACCTAGAT CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT
      241 AAACTTGGTC TGACAGTTAC CAATGCTTAA TCAGTGAGGC ACCTATCTCA GCGATCTGTC
      301 TATTTCGTTC ATCCATAGTT GCCTGACTCC CCGTCGTGTA GATAACTACG ATACGGGAGG
      361 GCTTACCATC TGGCCCCAGT GCTGCAATGA TACCGCGAGA CCCACGCTCA CCGGCTCCAG
      421 ATTTATCAGC AATAAACCAG CCAGCCGGAA GGGCCGAGCG CAGAAGTGGT CCTGCAACTT
      481 TATCCGCCTC CATCCAGTCT ATTAATTGTT GCCGGGAAGC TAGAGTAAGT AGTTCGCCAG
      541 TTAATAGTTT GCGCAACGTT GTTGCCATTG CTACAGGCAT CGTGGTGTCA CGCTCGTCGT
      601 TTGGTATGGC TTCATTCAGC TCCGGTTCCC AACGATCAAG GCGAGTTACA TGATCCCCCA
      661 TGTTGTGCAA AAAAGCGGTT AGCTCCTTCG GTCCTCCGAT CGTTGTCAGA AGTAAGTTGG
      721 CCGCAGTGTT ATCACTCATG GTTATGGCAG CACTGCATAA TTCTCTTACT GTCATGCCAT
      781 CCGTAAGATG CTTTTCTGTG ACTGGTGAGT ACTCAACCAA GTCATTCTGA GAATAGTGTA
      841 TGCGGCGACC GAGTTGCTCT TGCCCGGCGT CAATACGGGA TAATACCGCG CCACATAGCA
      901 GAACTTTAAA AGTGCTCATC ATTGGAAAAC GTTCTTCGGG GCGAAAACTC TCAAGGATCT
      961 TACCGCTGTT GAGATCCAGT TCGATGTAAC CCACTCGTGC ACCCAACTGA TCTTCAGCAT
     1021 CTTTTACTTT CACCAGCGTT TCTGGGTGAG CAAAAACAGG AAGGCAAAAT GCCGCAAAAA
     1081 AGGGAATAAG GGCGACACGG AAATGTTGAA TACTCATACT CTTCCTTTTT CAATATTATT
     1141 GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT ATTTAGAAAA
     1201 ATAAACAAAT AGGGGTTCCG CGCACATTTC CCCGAAAAGT GCCACCTGAC GTCTAAGAAA
     1261 CCATTATTAT CATGACATTA ACCTATAAAA ATAGGCGTAT CACGAGGCCC TTTCGTCTTC
     1321 ACCTCGAGAA ATCATAAAAA ATTTATTTGC TTTGTGAGCG GATAACAATT ATAATAGATT
     1381 CAATTGTGAG CGGATAACAA TTTCACACAG AATTCTCTTA GAGGGACAAT CGATGTCTAA
     1441 AGAAAAGTTT GAACGTACAA AACCGCACGT TAACGTCGGT ACTATCGGCC ACGTTGACCA
     1501 TGGTAAAACA ACGCTGACCG CTGCAATCAC TACCGTACTG GCTAAAACCT ACGGCGGTGC
     1561 TGCTCGCGCA TTCGACCAGA TCGATAACGC GCCGGAAGAA AAAGCTCGTG GTATCACCAT
     1621 CAACACTTCT CACGTTGAAT ACGACACCCC GACCCGTCAC TACGCACACG TAGACTGCCC
     1681 GGGGCACGCC GACTATGTTA AAAACATGAT CACCGGTGCT GCGCAGATGG ACGGCGCGAT
     1741 CCTGGTAGTT GCTGCGACTG ACGGCCCGAT GCCGCAGACT CGTGAGCACA TCCTGCTGGG
     1801 TCGTCAGGTA GGCGTTCCGT ACATCATCGT GTTCCTGAAC AAATGCGACA TGGTTGATGA
     1861 CGAAGAGCTG CTGGAACTGG TTGAAATGGA AGTTCGTGAA CTTCTGTCTC AGTACGACTT
     1921 CCCGGGCGAC GACACTCCGA TCGTTCGTGG TTCTGCTCTG AAAGCGCTGG AAGGCGACGC
     1981 AGAGTGGGAA GCGAAAATCC TGGAACTGGC TGGCTTCCTG GATTCTTACA TTCCGGAACC
     2041 AGAGCGTGCG ATTGACAAGC CGTTCCTGCT GCCGATCGAA GACGTATTCT CCATCTCCGG
     2101 TCGTGGTACC GTTGTTACCG GTCGTGTAGA ACGCGGTATC ATCAAAGTTG GTGAAGAAGT
     2161 TGAAATCGTT GGTATCAAAG AGACTCAGAA GTCTACCTGT ACTGGCGTTG AAATGTTCCG
     2221 CAAACTGCTG GACGAAGGCC GTGCTGGTGA GAACGTAGGT GTTCTGCTGC GTGGTATCAA
     2281 ACGTGAAGAA ATCGAACGTG GTCAGGTACT GGCTAAGCCG GGCACCATCA AGCCGCACAC
     2341 CAAGTTCGAA TCTGAAGTGT ACATTCTGTC CAAAGATGAA GGCGGCCGTC ATACTCCGTT
     2401 CTTCAAAGGC TACCGTCCGC AGTTCTACTT CCGTACTACT GACGTGACTG GTACCATCGA
     2461 ACTGCCGGAA GGCGTAGAGA TGGTAATGCC GGGCGACAAC ATCAAAATGG TTGTTACCCT
     2521 GATCCACCCG ATCGCGATGG ACGACGGTCT GCGTTTCGCA ATCCGTGAAG GCGGCCGTAC
     2581 CGTTGGCGCG GGCGTTGTAG CAAAAGTTCT GGGCAGATCT CATCACCATC ACCATCACTA
     2641 AGCTTAATTA GCTGAGCTTG GACTCCTGTT GATAGATCCA GTAATGACCT CAGAACTCCA
     2701 TCTGGATTTG TTCAGAACGC TCGGTTGCCG CCGGGCGTTT TTTATTGGTG AGAATCCAAG
     2761 CTAGCTTGGC GAGATTTTCA GGAGCTAAGG AAGCTAAAAT GGAGAAAAAA ATCACTGGAT
     2821 ATACCACCGT TGATATATCC CAATGGCATC GTAAAGAACA TTTTGAGGCA TTTCAGTCAG
     2881 TTGCTCAATG TACCTATAAC CAGACCGTTC AGCTGGATAT TACGGCCTTT TTAAAGACCG
     2941 TAAAGAAAAA TAAGCACAAG TTTTATCCGG CCTTTATTCA CATTCTTGCC CGCCTGATGA
     3001 ATGCTCATCC GGAATTTCGT ATGGCAATGA AAGACGGTGA GCTGGTGATA TGGGATAGTG
     3061 TTCACCCTTG TTACACCGTT TTCCATGAGC AAACTGAAAC GTTTTCATCG CTCTGGAGTG
     3121 AATACCACGA CGATTTCCGG CAGTTTCTAC ACATATATTC GCAAGATGTG GCGTGTTACG
     3181 GTGAAAACCT GGCCTATTTC CCTAAAGGGT TTATTGAGAA TATGTTTTTC GTCTCAGCCA
     3241 ATCCCTGGGT GAGTTTCACC AGTTTTGATT TAAACGTGGC CAATATGGAC AACTTCTTCG
     3301 CCCCCGTTTT CACCATGCAT GGGCAAATAT TATACGCAAG GCGACAAGGT GCTGATGCCG
     3361 CTGGCGATTC AGGTTCATCA TGCCGTCTGT GATGGCTTCC ATGTCGGCAG AATGCTTAAT
     3421 GAATTACAAC AGTACTGCGA TGAGTGGCAG GGCGGGGCGT AATTTTTTTA AGGCAGTTAT
     3481 TGGTGCCCTT AAACGCCTGG GGTAATGACT CTCTAGCTTG AGGCATCAAA TAAAACGAAA
     3541 GGCTCAGTCG AAAGACTGGG CCTTTCGTTT TATCTGTTGT TTGTCGGTGA ACGCTCTCCT
     3601 GAGTAGGACA AATCCGCCGC TCTAGAGCTG CCTCGCGCGT TTCGGTGATG ACGGTGAAAA
     3661 CCTCTGACAC ATGCAGCTCC CGGAGACGGT CACAGCTTGT CTGTAAGCGG ATGCCGGGAG
     3721 CAGACAAGCC CGTCAGGGCG CGTCAGCGGG TGTTGGCGGG TGTCGGGGCG CAGCCATGAC
     3781 CCAGTCACGT AGCGATAGCG GAGTGTATAC TGGCTTAACT ATGCGGCATC AGAGCAGATT
     3841 GTACTGAGAG TGCACCATAT GCGGTGTGAA ATACCGCACA GATGCGTAAG GAGAAAATAC
     3901 CGCATCAGGC GCTCTTCCGC TTCCTCGCTC ACTGACTCGC TGCGCTCGGT CGTTCGGCTG
     3961 CGGCGAGCGG TATCAGCTCA CTCAAAGGCG GTAATACGGT TATCCACAGA ATCAGGGGAT
     4021 AACGCAGGAA AGAACATGTG AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC
     4081 GCGTTGCTGG CGTTTTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC
     4141 TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA
     4201 AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT
     4261 CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT AGCTCACGCT GTAGGTATCT CAGTTCGGTG
     4321 TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC
     4381 GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG
     4441 GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC
     4501 TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGGACAG TATTTGGTAT CTGCGCTCTG
     4561 CTGAAGCCAG TTACCTTCGG AAAAAGAGTT GGTAGCT
//