LOCUS       27_pQE-60               4513 bp ds-DNA     circular     18-FEB-2019
DEFINITION  .
KEYWORDS    "creator:Barbora" "marker:AmpR"                                    
FEATURES             Location/Qualifiers
     CDS             complement(4240..4308)
                     /label="AmpR"
     gene            151..1233
                     /label="RF1"
     rep_origin      complement(2689..3277)
                     /label="ori"
     promoter        31..47
                     /label="T5 promoter"
     terminator      2121..2207
                     /label="rrnB T1 terminator"
     promoter        25..30
                     /label="T5 promoter"
     misc_feature    2363..2503
                     /label="bom"
     CDS             complement(3448..4239)
                     /label="AmpR"
     promoter        48..54
                     /label="T5 promoter"
     RBS             97..108
                     /label="RBS"
     promoter        10..24
                     /label="T5 promoter"
     promoter        complement(4309..4413)
                     /label="AmpR promoter"
     protein_bind    62..78
                     /label="lac operator"
     protein_bind    30..46
                     /label="lac operator"
     terminator      1255..1349
                     /label="lambda t0 terminator"
ORIGIN
        1 CTCGAGAAAT CATAAAAAAT TTATTTGCTT TGTGAGCGGA TAACAATTAT AATAGATTCA
       61 ATTGTGAGCG GATAACAATT TCACACAGAA TTCATTAAAG AGGAGAAATT AACTATGAGA
      121 GGATCGCATC ACCATCACCA TCACGGATCC ATGAAGCCTT CTATCGTTGC CAAACTGGAA
      181 GCCCTGCATG AACGCCATGA AGAAGTTCAG GCGTTGCTGG GTGACGCGCA AACTATCGCC
      241 GACCAGGAAC GTTTTCGCGC ATTATCACGC GAATATGCGC AGTTAAGTGA TGTTTCGCGC
      301 TGTTTTACCG ACTGGCAACA GGTTCAGGAA GATATCGAAA CCGCACAGAT GATGCTCGAT
      361 GATCCTGAAA TGCGTGAGAT GGCGCAGGAT GAACTGCGCG AAGCTAAAGA AAAAAGCGAG
      421 CAACTGGAAC AGCAATTACA GGTTCTGTTA CTGCCAAAAG ATCCTGATGA CGAACGTAAC
      481 GCCTTCCTCG AAGTCCGAGC CGGAACCGGC GGCGACGAAG CGGCGCTGTT CGCGGGCGAT
      541 CTGTTCCGTA TGTACAGCCG TTATGCCGAA GCCCGCCGCT GGCGGGTAGA AATCATGAGC
      601 GCCAGCGAGG GTGAACATGG TGGTTATAAA GAGATCATCG CCAAAATTAG CGGTGATGGT
      661 GTGTATGGTC GTCTGAAATT TGAATCCGGC GGTCATCGCG TGCAACGTGT TCCTGCTACG
      721 GAATCGCAGG GTCGTATTCA TACTTCTGCT TGTACCGTTG CGGTAATGCC AGAACTGCCT
      781 GACGCAGAAC TGCCGGACAT CAACCCAGCA GATTTACGCA TTGATACTTT CCGCTCGTCA
      841 GGGGCGGGTG GTCAGCACGT TAACACCACC GATTCGGCAA TTCGTATTAC TCACTTGCCG
      901 ACCGGGATTG TTGTTGAATG TCAGGACGAA CGTTCACAAC ATAAAAACAA AGCTAAAGCA
      961 CTTTCTGTTC TCGGTGCTCG CATCCACGCT GCTGAAATGG CAAAACGCCA ACAGGCCGAA
     1021 GCGTCTACCC GTCGTAACCT GCTGGGGAGT GGCGATCGCA GCGACCGTAA CCGTACTTAC
     1081 AACTTCCCGC AGGGGCGCGT TACCGATCAC CGCATCAACC TGACGCTCTA CCGCCTGGAT
     1141 GAAGTGATGG AAGGTAAGCT GGATATGCTG ATTGAACCGA TTATCCAGGA ACATCAGGCC
     1201 GACCAACTGG CGGCGTTGTC CGAGCAGGAA TAAAAGCTTA ATTAGCTGAG CTTGGACTCC
     1261 TGTTGATAGA TCCAGTAATG ACCTCAGAAC TCCATCTGGA TTTGTTCAGA ACGCTCGGTT
     1321 GCCGCCGGGC GTTTTTTATT GGTGAGAATC CAAGCTAGCT TGGCGAGATT TTCAGGAGCT
     1381 AAGGAAGCTA AAATGGAGAA AAAAATCACT GGATATACCA CCGTTGATAT ATCCCAATGG
     1441 CATCGTAAAG AACATTTTGA GGCATTTCAG TCAGTTGCTC AATGTACCTA TAACCAGACC
     1501 GTTCAGCTGG ATATTACGGC CTTTTTAAAG ACCGTAAAGA AAAATAAGCA CAAGTTTTAT
     1561 CCGGCCTTTA TTCACATTCT TGCCCGCCTG ATGAATGCTC ATCCGGAATT TCGTATGGCA
     1621 ATGAAAGACG GTGAGCTGGT GATATGGGAT AGTGTTCACC CTTGTTACAC CGTTTTCCAT
     1681 GAGCAAACTG AAACGTTTTC ATCGCTCTGG AGTGAATACC ACGACGATTT CCGGCAGTTT
     1741 CTACACATAT ATTCGCAAGA TGTGGCGTGT TACGGTGAAA ACCTGGCCTA TTTCCCTAAA
     1801 GGGTTTATTG AGAATATGTT TTTCGTCTCA GCCAATCCCT GGGTGAGTTT CACCAGTTTT
     1861 GATTTAAACG TGGCCAATAT GGACAACTTC TTCGCCCCCG TTTTCACCAT GCATGGGCAA
     1921 ATATTATACG CAAGGCGACA AGGTGCTGAT GCCGCTGGCG ATTCAGGTTC ATCATGCCGT
     1981 CTGTGATGGC TTCCATGTCG GCAGAATGCT TAATGAATTA CAACAGTACT GCGATGAGTG
     2041 GCAGGGCGGG GCGTAATTTT TTTAAGGCAG TTATTGGTGC CCTTAAACGC CTGGGGTAAT
     2101 GACTCTCTAG CTTGAGGCAT CAAATAAAAC GAAAGGCTCA GTCGAAAGAC TGGGCCTTTC
     2161 GTTTTATCTG TTGTTTGTCG GTGAACGCTC TCCTGAGTAG GACAAATCCG CCGCTCTAGA
     2221 GCTGCCTCGC GCGTTTCGGT GATGACGGTG AAAACCTCTG ACACATGCAG CTCCCGGAGA
     2281 CGGTCACAGC TTGTCTGTAA GCGGATGCCG GGAGCAGACA AGCCCGTCAG GGCGCGTCAG
     2341 CGGGTGTTGG CGGGTGTCGG GGCGCAGCCA TGACCCAGTC ACGTAGCGAT AGCGGAGTGT
     2401 ATACTGGCTT AACTATGCGG CATCAGAGCA GATTGTACTG AGAGTGCACC ATATGCGGTG
     2461 TGAAATACCG CACAGATGCG TAAGGAGAAA ATACCGCATC AGGCGCTCTT CCGCTTCCTC
     2521 GCTCACTGAC TCGCTGCGCT CGGTCGTTCG GCTGCGGCGA GCGGTATCAG CTCACTCAAA
     2581 GGCGGTAATA CGGTTATCCA CAGAATCAGG GGATAACGCA GGAAAGAACA TGTGAGCAAA
     2641 AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT
     2701 CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC
     2761 AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC
     2821 GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC
     2881 TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG
     2941 TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA
     3001 GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG
     3061 CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA
     3121 CACTAGAAGG ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG
     3181 AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG
     3241 CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GATCCTTTGA TCTTTTCTAC
     3301 GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGAGATTATC
     3361 AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA AGTTTTAAAT CAATCTAAAG
     3421 TATATATGAG TAAACTTGGT CTGACAGTTA CCAATGCTTA ATCAGTGAGG CACCTATCTC
     3481 AGCGATCTGT CTATTTCGTT CATCCATAGT TGCCTGACTC CCCGTCGTGT AGATAACTAC
     3541 GATACGGGAG GGCTTACCAT CTGGCCCCAG TGCTGCAATG ATACCGCGAG ACCCACGCTC
     3601 ACCGGCTCCA GATTTATCAG CAATAAACCA GCCAGCCGGA AGGGCCGAGC GCAGAAGTGG
     3661 TCCTGCAACT TTATCCGCCT CCATCCAGTC TATTAATTGT TGCCGGGAAG CTAGAGTAAG
     3721 TAGTTCGCCA GTTAATAGTT TGCGCAACGT TGTTGCCATT GCTACAGGCA TCGTGGTGTC
     3781 ACGCTCGTCG TTTGGTATGG CTTCATTCAG CTCCGGTTCC CAACGATCAA GGCGAGTTAC
     3841 ATGATCCCCC ATGTTGTGCA AAAAAGCGGT TAGCTCCTTC GGTCCTCCGA TCGTTGTCAG
     3901 AAGTAAGTTG GCCGCAGTGT TATCACTCAT GGTTATGGCA GCACTGCATA ATTCTCTTAC
     3961 TGTCATGCCA TCCGTAAGAT GCTTTTCTGT GACTGGTGAG TACTCAACCA AGTCATTCTG
     4021 AGAATAGTGT ATGCGGCGAC CGAGTTGCTC TTGCCCGGCG TCAATACGGG ATAATACCGC
     4081 GCCACATAGC AGAACTTTAA AAGTGCTCAT CATTGGAAAA CGTTCTTCGG GGCGAAAACT
     4141 CTCAAGGATC TTACCGCTGT TGAGATCCAG TTCGATGTAA CCCACTCGTG CACCCAACTG
     4201 ATCTTCAGCA TCTTTTACTT TCACCAGCGT TTCTGGGTGA GCAAAAACAG GAAGGCAAAA
     4261 TGCCGCAAAA AAGGGAATAA GGGCGACACG GAAATGTTGA ATACTCATAC TCTTCCTTTT
     4321 TCAATATTAT TGAAGCATTT ATCAGGGTTA TTGTCTCATG AGCGGATACA TATTTGAATG
     4381 TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCACATTT CCCCGAAAAG TGCCACCTGA
     4441 CGTCTAAGAA ACCATTATTA TCATGACATT AACCTATAAA AATAGGCGTA TCACGAGGCC
     4501 CTTTCGTCTT CAC
//