LOCUS       28_pET-15b              6798 bp ds-DNA     circular     20-FEB-2019
DEFINITION  .
KEYWORDS    "creator:Barbora" "marker:AmpR"                                    
FEATURES             Location/Qualifiers
     protein_bind    1526..1550
                     /label="lac operator"
     misc_feature    4643..4785
                     /label="bom"
     CDS             4350..4541
                     /label="rop"
     gene            complement(325..1421)
                     /label="RF2"
     promoter        33..38
                     /label="tet promoter"
     CDS             complement(5730..6521)
                     /label="AmpR"
     misc_difference 685..687
                     /label="Mutation A246T"
     CDS             complement(1453..1470)
                     /label="6xHis"
     rep_origin      complement(4971..5559)
                     /label="ori"
     RBS             1489..1511
                     /label="RBS"
     promoter        10..15
                     /label="tet promoter"
     promoter        16..32
                     /label="tet promoter"
     CDS             complement(6522..6590)
                     /label="AmpR"
     CDS             complement(1426..1443)
                     /label="thrombin site"
     promoter        complement(1551..1569)
                     /label="T7 promoter"
     promoter        complement(6591..6695)
                     /label="AmpR promoter"
     promoter        1878..1955
                     /label="lacI promoter"
     terminator      213..260
                     /label="T7 terminator"
     CDS             1956..3038
                     /label="lacI"
     D-loop          667..675
                     /label="GGQ motif"
ORIGIN
        1 ttctcatgtt tgacagctta tcatcgataa gctttaatgc ggtagtttat cacagttaaa
       61 ttgctaacgc agtcaggcac cgtgtatgaa atctaacaat gcgctcatcg tcatcctcgg
      121 caccgtcacc ctggatgctg taggcatagg cttggttatg ccggtactgc cgggcctctt
      181 gcgggatatc cggatatagt tcctcctttc agcaaaaaac ccctcaagac ccgtttagag
      241 gccccaaggg gttatgctag ttattgctca gcggtggcag cagccaactc agcttccttt
      301 cgggctttgt tagcagccgG ATCCTCATAA CCCTGCTTTC AAACTTGCTT CGATAAATTG
      361 ATCCAGGCTG CCGTCCAGCA CGGCCTGCGT GTTGCGGGTT TCTACCCCGG TGCGCAGATC
      421 TTTAATGCGG GAGTCATCAA GGACATAAGA ACGAATCTGG CTGCCCCAGC CGATGTCGGA
      481 TTTGTTATCT TCCATCGCCT GTTTCTCGGC ATTTTTCTTC TGCATCTCCA GTTCATAAAG
      541 CTTCGCTTTC ATCTGCTTCA TGGCCTGATC TTTGTTCTTG TGCTGGGAAC GGTCGTTCTG
      601 GCACTGGGTC ACGATCCCGG TCGGGATGTG GGTAATACGC ACCGCAGATT CGGTACGGTT
      661 AACGTGCTGA CCGCCCGCGC CGGACGTGCG ATAAACGTCA ATGCGCAGAT CCGCCGGGTT
      721 GATTTCGATA TCAATATCAT CATCAACTTC CGGATAAACA AACGCGGAGC TGAACGACGT
      781 GTGGCGACGA CCGCCGGAGT CAAACGGGCT TTTACGCACC AGGCGGTGAA CGCCGGTTTC
      841 TGTACGCAGC CAGCCGTAAG CGTAATCGCC GGAGATTTTG ATCGTCACGG ATTTAATACC
      901 CGCCACTTCA CCTTCCGACT CTTCGATGAT TTCAGTTTTG AAACCACGCG ATTCTGCCCA
      961 GCGCAGATAC ATACGCTCAA GCATGCTCGC CCAGTCCTGT GCTTCCGTAC CGCCAGACCC
     1021 CGCCTGAATA TCGAGGTAGC AGTCGGCGCT GTCATATTCG CCAGAGAACA TACGGCGGAA
     1081 CTCAAGCTGC GCCAGTTTTT CTTCCAGGGC GTCGAGTTCA GCAACGGCTT CGTTAAAGGT
     1141 TTCTTCGTCG TCAGCTTCTA CAGCCAGTTC CAGCAGACCA GAAACATCTT CCAGCCCCTG
     1201 TTTCATTTGG TCGAGGGTGT CGACAACGGC TTCGAGGGAG GAACGCTCTT TACCCAGCGC
     1261 CTGTGCGCGT TCGGGTTCGT TCCAGACATC CGGCTGTTCC AGCTCGGCGT TTACTTCTTC
     1321 CAGACGCTCT TTCTTGGCGT CGTAGTCAAG ATACCCCCTA AGAACGTCGG AGCGTTCCGT
     1381 GAGGTCCTGA ATGCGATTAT TTACCGGATT AATTTCAAAC Atatggctgc cgcgcggcac
     1441 caggccgctg ctgtgatgat gatgatgatg gctgctgccc atggtatatc tccttcttaa
     1501 agttaaacaa aattatttct agaggggaat tgttatccgc tcacaattcc cctatagtga
     1561 gtcgtattaa tttcgcggga tcgagatctc gatcctctac gccggacgca tcgtggccgg
     1621 catcaccggc gccacaggtg cggttgctgg cgcctatatc gccgacatca ccgatgggga
     1681 agatcgggct cgccacttcg ggctcatgag cgcttgtttc ggcgtgggta tggtggcagg
     1741 ccccgtggcc gggggactgt tgggcgccat ctccttgcat gcaccattcc ttgcggcggc
     1801 ggtgctcaac ggcctcaacc tactactggg ctgcttccta atgcaggagt cgcataaggg
     1861 agagcgtcga gatcccggac accatcgaat ggcgcaaaac ctttcgcggt atggcatgat
     1921 agcgcccgga agagagtcaa ttcagggtgg tgaatgtgaa accagtaacg ttatacgatg
     1981 tcgcagagta tgccggtgtc tcttatcaga ccgtttcccg cgtggtgaac caggccagcc
     2041 acgtttctgc gaaaacgcgg gaaaaagtgg aagcggcgat ggcggagctg aattacattc
     2101 ccaaccgcgt ggcacaacaa ctggcgggca aacagtcgtt gctgattggc gttgccacct
     2161 ccagtctggc cctgcacgcg ccgtcgcaaa ttgtcgcggc gattaaatct cgcgccgatc
     2221 aactgggtgc cagcgtggtg gtgtcgatgg tagaacgaag cggcgtcgaa gcctgtaaag
     2281 cggcggtgca caatcttctc gcgcaacgcg tcagtgggct gatcattaac tatccgctgg
     2341 atgaccagga tgccattgct gtggaagctg cctgcactaa tgttccggcg ttatttcttg
     2401 atgtctctga ccagacaccc atcaacagta ttattttctc ccatgaagac ggtacgcgac
     2461 tgggcgtgga gcatctggtc gcattgggtc accagcaaat cgcgctgtta gcgggcccat
     2521 taagttctgt ctcggcgcgt ctgcgtctgg ctggctggca taaatatctc actcgcaatc
     2581 aaattcagcc gatagcggaa cgggaaggcg actggagtgc catgtccggt tttcaacaaa
     2641 ccatgcaaat gctgaatgag ggcatcgttc ccactgcgat gctggttgcc aacgatcaga
     2701 tggcgctggg cgcaatgcgc gccattaccg agtccgggct gcgcgttggt gcggatatct
     2761 cggtagtggg atacgacgat accgaagaca gctcatgtta tatcccgccg ttaaccacca
     2821 tcaaacagga ttttcgcctg ctggggcaaa ccagcgtgga ccgcttgctg caactctctc
     2881 agggccaggc ggtgaagggc aatcagctgt tgcccgtctc actggtgaaa agaaaaacca
     2941 ccctggcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc
     3001 tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtaagt
     3061 tagctcactc attaggcacc gggatctcga ccgatgccct tgagagcctt caacccagtc
     3121 agctccttcc ggtgggcgcg gggcatgact atcgtcgccg cacttatgac tgtcttcttt
     3181 atcatgcaac tcgtaggaca ggtgccggca gcgctctggg tcattttcgg cgaggaccgc
     3241 tttcgctgga gcgcgacgat gatcggcctg tcgcttgcgg tattcggaat cttgcacgcc
     3301 ctcgctcaag ccttcgtcac tggtcccgcc accaaacgtt tcggcgagaa gcaggccatt
     3361 atcgccggca tggcggccga cgcgctgggc tacgtcttgc tggcgttcgc gacgcgaggc
     3421 tggatggcct tccccattat gattcttctc gcttccggcg gcatcgggat gcccgcgttg
     3481 caggccatgc tgtccaggca ggtagatgac gaccatcagg gacagcttca aggatcgctc
     3541 gcggctctta ccagcctaac ttcgatcact ggaccgctga tcgtcacggc gatttatgcc
     3601 gcctcggcga gcacatggaa cgggttggca tggattgtag gcgccgccct ataccttgtc
     3661 tgcctccccg cgttgcgtcg cggtgcatgg agccgggcca cctcgacctg aatggaagcc
     3721 ggcggcacct cgctaacgga ttcaccactc caagaattgg agccaatcaa ttcttgcgga
     3781 gaactgtgaa tgcgcaaacc aacccttggc agaacatatc catcgcgtcc gccatctcca
     3841 gcagccgcac gcggcgcatc tcgggcagcg ttgggtcctg gccacgggtg cgcatgatcg
     3901 tgctcctgtc gttgaggacc cggctaggct ggcggggttg ccttactggt tagcagaatg
     3961 aatcaccgat acgcgagcga acgtgaagcg actgctgctg caaaacgtct gcgacctgag
     4021 caacaacatg aatggtcttc ggtttccgtg tttcgtaaag tctggaaacg cggaagtcag
     4081 cgccctgcac cattatgttc cggatctgca tcgcaggatg ctgctggcta ccctgtggaa
     4141 cacctacatc tgtattaacg aagcgctggc attgaccctg agtgattttt ctctggtccc
     4201 gccgcatcca taccgccagt tgtttaccct cacaacgttc cagtaaccgg gcatgttcat
     4261 catcagtaac ccgtatcgtg agcatcctct ctcgtttcat cggtatcatt acccccatga
     4321 acagaaatcc cccttacacg gaggcatcag tgaccaaaca ggaaaaaacc gcccttaaca
     4381 tggcccgctt tatcagaagc cagacattaa cgcttctgga gaaactcaac gagctggacg
     4441 cggatgaaca ggcagacatc tgtgaatcgc ttcacgacca cgctgatgag ctttaccgca
     4501 gctgcctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga
     4561 cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag
     4621 cgggtgttgg cgggtgtcgg ggcgcagcca tgacccagtc acgtagcgat agcggagtgt
     4681 atactggctt aactatgcgg catcagagca gattgtactg agagtgcacc atatatgcgg
     4741 tgtgaaatac cgcacagatg cgtaaggaga aaataccgca tcaggcgctc ttccgcttcc
     4801 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
     4861 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
     4921 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     4981 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     5041 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     5101 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     5161 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     5221 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     5281 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     5341 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     5401 tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     5461 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     5521 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     5581 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
     5641 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
     5701 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
     5761 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
     5821 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
     5881 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
     5941 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
     6001 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctgcagg catcgtggtg
     6061 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
     6121 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
     6181 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
     6241 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
     6301 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaacacg ggataatacc
     6361 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
     6421 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
     6481 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
     6541 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
     6601 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
     6661 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
     6721 gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg
     6781 ccctttcgtc ttcaagaa
//