LOCUS       30_pQE-60               3978 bp ds-DNA     circular     18-FEB-2019
DEFINITION  .
KEYWORDS    "creator:Barbora" "marker:AmpR"                                    
FEATURES             Location/Qualifiers
     CDS             2280..2297
                     /label="6xHis"
     CDS             complement(535..1326)
                     /label="AmpR"
     protein_bind    1662..1678
                     /label="lac operator"
     promoter        1625..1630
                     /label="T5 promoter"
     promoter        1610..1624
                     /label="T5 promoter"
     rep_origin      complement(join(3754..3978,1..364))
                     /label="ori"
     terminator      3186..3272
                     /label="rrnB T1 terminator"
     promoter        1631..1647
                     /label="T5 promoter"
     CDS             complement(1327..1395)
                     /label="AmpR"
     promoter        1648..1654
                     /label="T5 promoter"
     promoter        complement(1396..1500)
                     /label="AmpR promoter"
     protein_bind    1630..1646
                     /label="lac operator"
     misc_feature    3428..3568
                     /label="bom"
     terminator      2320..2414
                     /label="lambda t0 terminator"
     misc_feature    1713..2268
                     /label="RRF"
ORIGIN
        1 GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG
       61 CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT
      121 GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT
      181 CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGGACA GTATTTGGTA TCTGCGCTCT
      241 GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC
      301 CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC
      361 TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG
      421 TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA
      481 AAAATGAAGT TTTAAATCAA TCTAAAGTAT ATATGAGTAA ACTTGGTCTG ACAGTTACCA
      541 ATGCTTAATC AGTGAGGCAC CTATCTCAGC GATCTGTCTA TTTCGTTCAT CCATAGTTGC
      601 CTGACTCCCC GTCGTGTAGA TAACTACGAT ACGGGAGGGC TTACCATCTG GCCCCAGTGC
      661 TGCAATGATA CCGCGAGACC CACGCTCACC GGCTCCAGAT TTATCAGCAA TAAACCAGCC
      721 AGCCGGAAGG GCCGAGCGCA GAAGTGGTCC TGCAACTTTA TCCGCCTCCA TCCAGTCTAT
      781 TAATTGTTGC CGGGAAGCTA GAGTAAGTAG TTCGCCAGTT AATAGTTTGC GCAACGTTGT
      841 TGCCATTGCT ACAGGCATCG TGGTGTCACG CTCGTCGTTT GGTATGGCTT CATTCAGCTC
      901 CGGTTCCCAA CGATCAAGGC GAGTTACATG ATCCCCCATG TTGTGCAAAA AAGCGGTTAG
      961 CTCCTTCGGT CCTCCGATCG TTGTCAGAAG TAAGTTGGCC GCAGTGTTAT CACTCATGGT
     1021 TATGGCAGCA CTGCATAATT CTCTTACTGT CATGCCATCC GTAAGATGCT TTTCTGTGAC
     1081 TGGTGAGTAC TCAACCAAGT CATTCTGAGA ATAGTGTATG CGGCGACCGA GTTGCTCTTG
     1141 CCCGGCGTCA ATACGGGATA ATACCGCGCC ACATAGCAGA ACTTTAAAAG TGCTCATCAT
     1201 TGGAAAACGT TCTTCGGGGC GAAAACTCTC AAGGATCTTA CCGCTGTTGA GATCCAGTTC
     1261 GATGTAACCC ACTCGTGCAC CCAACTGATC TTCAGCATCT TTTACTTTCA CCAGCGTTTC
     1321 TGGGTGAGCA AAAACAGGAA GGCAAAATGC CGCAAAAAAG GGAATAAGGG CGACACGGAA
     1381 ATGTTGAATA CTCATACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG
     1441 TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG
     1501 CACATTTCCC CGAAAAGTGC CACCTGACGT CTAAGAAACC ATTATTATCA TGACATTAAC
     1561 CTATAAAAAT AGGCGTATCA CGAGGCCCTT TCGTCTTCAC CTCGAGAAAT CATAAAAAAT
     1621 TTATTTGCTT TGTGAGCGGA TAACAATTAT AATAGATTCA ATTGTGAGCG GATAACAATT
     1681 TCACACAGAA TTCGTTTTCA AGGATTCGTA ACATGATTAG CGATATCAGA AAAGATGCTG
     1741 AAGTACGCAT GGACAAATGC GTAGAAGCGT TCAAAACCCA AATCAGCAAA ATACGCACGG
     1801 GTCGTGCTTC TCCCAGCCTG CTGGATGGCA TTGTCGTGGA ATATTACGGC ACGCCGACGC
     1861 CGCTGCGTCA GCTGGCAAGC GTAACGGTAG AAGATTCCCG TACACTGAAA ATCAACGTGT
     1921 TTGATCGTTC AATGTCTCCG GCCGTTGAAA AAGCGATTAT GGCGTCCGAT CTTGGCCTGA
     1981 ACCCGAACTC TGCGGGTAGC GACATCCGTG TTCCGCTGCC GCCGCTGACG GAAGAACGTC
     2041 GTAAAGATCT GACCAAAATC GTTCGTGGTG AAGCAGAACA AGCGCGTGTT GCAGTACGTA
     2101 ACGTGCGTCG TGACGCGAAC GACAAAGTGA AAGCACTGTT GAAAGATAAA GAGATCAGCG
     2161 AAGACGACGA TCGCCGTTCT CAGGACGATG TACAGAAACT GACTGATGCT GCAATCAAGA
     2221 AAATTGAAGC GGCGCTGGCA GACAAAGAAG CAGAACTGAT GCAGTTCGGA TCCAGATCTC
     2281 ATCACCATCA CCATCACTAA GCTTAATTAG CTGAGCTTGG ACTCCTGTTG ATAGATCCAG
     2341 TAATGACCTC AGAACTCCAT CTGGATTTGT TCAGAACGCT CGGTTGCCGC CGGGCGTTTT
     2401 TTATTGGTGA GAATCCAAGC TAGCTTGGCG AGATTTTCAG GAGCTAAGGA AGCTAAAATG
     2461 GAGAAAAAAA TCACTGGATA TACCACCGTT GATATATCCC AATGGCATCG TAAAGAACAT
     2521 TTTGAGGCAT TTCAGTCAGT TGCTCAATGT ACCTATAACC AGACCGTTCA GCTGGATATT
     2581 ACGGCCTTTT TAAAGACCGT AAAGAAAAAT AAGCACAAGT TTTATCCGGC CTTTATTCAC
     2641 ATTCTTGCCC GCCTGATGAA TGCTCATCCG GAATTTCGTA TGGCAATGAA AGACGGTGAG
     2701 CTGGTGATAT GGGATAGTGT TCACCCTTGT TACACCGTTT TCCATGAGCA AACTGAAACG
     2761 TTTTCATCGC TCTGGAGTGA ATACCACGAC GATTTCCGGC AGTTTCTACA CATATATTCG
     2821 CAAGATGTGG CGTGTTACGG TGAAAACCTG GCCTATTTCC CTAAAGGGTT TATTGAGAAT
     2881 ATGTTTTTCG TCTCAGCCAA TCCCTGGGTG AGTTTCACCA GTTTTGATTT AAACGTGGCC
     2941 AATATGGACA ACTTCTTCGC CCCCGTTTTC ACCATGCATG GGCAAATATT ATACGCAAGG
     3001 CGACAAGGTG CTGATGCCGC TGGCGATTCA GGTTCATCAT GCCGTCTGTG ATGGCTTCCA
     3061 TGTCGGCAGA ATGCTTAATG AATTACAACA GTACTGCGAT GAGTGGCAGG GCGGGGCGTA
     3121 ATTTTTTTAA GGCAGTTATT GGTGCCCTTA AACGCCTGGG GTAATGACTC TCTAGCTTGA
     3181 GGCATCAAAT AAAACGAAAG GCTCAGTCGA AAGACTGGGC CTTTCGTTTT ATCTGTTGTT
     3241 TGTCGGTGAA CGCTCTCCTG AGTAGGACAA ATCCGCCGCT CTAGAGCTGC CTCGCGCGTT
     3301 TCGGTGATGA CGGTGAAAAC CTCTGACACA TGCAGCTCCC GGAGACGGTC ACAGCTTGTC
     3361 TGTAAGCGGA TGCCGGGAGC AGACAAGCCC GTCAGGGCGC GTCAGCGGGT GTTGGCGGGT
     3421 GTCGGGGCGC AGCCATGACC CAGTCACGTA GCGATAGCGG AGTGTATACT GGCTTAACTA
     3481 TGCGGCATCA GAGCAGATTG TACTGAGAGT GCACCATATG CGGTGTGAAA TACCGCACAG
     3541 ATGCGTAAGG AGAAAATACC GCATCAGGCG CTCTTCCGCT TCCTCGCTCA CTGACTCGCT
     3601 GCGCTCGGTC GTTCGGCTGC GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG TAATACGGTT
     3661 ATCCACAGAA TCAGGGGATA ACGCAGGAAA GAACATGTGA GCAAAAGGCC AGCAAAAGGC
     3721 CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA
     3781 GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA
     3841 CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC
     3901 CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG
     3961 TAGGTATCTC AGTTCGGT
//